• Title/Summary/Keyword: zinc database

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Mechanical characteristic of ACSR Transmission lines by forest fires (산불에 열화된 ACSR 전선의 기계적 특성 검토)

  • Kang, J.W.;Jang, T.I.;Hong, D.S.;Park, C.G.;Bang, H.K.;Kim, Y.D.
    • Proceedings of the KIEE Conference
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    • 2003.07a
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    • pp.506-508
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    • 2003
  • In the case of a fire in the mountains, steel wires coated with zinc and aluminum wires of those power cables exposed to the fire or near around will be deteriorated by the blaze and the high temperature. This paper shows the experimental results that apply to a new wire by an artificial flame-maker and results including the intensity of extension and wrench for a conductor. In addition, there's been an experiment and analysis about the mechanical characteristics of the wire of ACSR $480mm^2$ which was removed from A T/L by a forest fire. Then, the database will be made to predict the state of deteriorated wires by a forest fire using those two data, and data necessary to diagnose the life state of an ACSR wire affected by a forest fire will be given.

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Pharmacophore Based Screening and Molecular Docking Study of PI3K Inhibitors

  • Rupa, Mottadi;Madhavan, Thirumurthy
    • Journal of Integrative Natural Science
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    • v.9 no.1
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    • pp.41-61
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    • 2016
  • Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related mortality worldwide. Phosphoinositide 3-kinases (PI3Ks) play important role in Non-Small Cell Lung Cancer. PI3Ks constitute a lipid kinase family which modulates the function of numerous substrates involved in the regulation of cell survival, cell cycle progression and cellular growth. Herein, we describe the ligand based pharmacophore combined with molecular docking studies methods to identify new potent PI3K inhibitors. Several pharmacophore models were generated and validated by Guner-Henry scoring Method. The best models were utilized as 3D pharmacophore query to screen against ZINC database (Chemical and Natural) and the retrieved hits were further validated by fitness score, Lipinski's rule of five. Finally four compounds were found to have good potential and they may act as novel lead compounds for PI3K inhibitor designing.

Characterization of a Stress-Responsive Ankyrin Repeat-Containing Zinc Finger Protein of Capsicum annuum (CaKR1)

  • Seong, Eun-Soo;Choi, Do-Il;Cho, Hye-Sun;Lim, Chun-Keum;Cho, Hye-Jeong;Wang, Myeong-Hyeon
    • BMB Reports
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    • v.40 no.6
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    • pp.952-958
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    • 2007
  • We isolated many genes induced from pepper cDNA microarray data following their infection with the soybean pustule pathogen Xanthomonas axonopodis pv. glycines 8ra. A full-length cDNA clone of the Capsicum annuum ankyrin-repeat domain $C_3H_1$ zinc finger protein (CaKR1) was identified in a chili pepper using the expressed sequence tag (EST) database. The deduced amino acid sequence of CaKR1 showed a significant sequence similarity (46%) to the ankyrin-repeat protein in very diverse family of proteins of Arabidopsis. The gene was induced in response to various biotic and abiotic stresses in the pepper leaves, as well as by an incompatible pathogen, such as salicylic acid (SA) and ethephon. CaKR1 expression was highest in the root and flower, and its expression was induced by treatment with agents such as NaCl and methyl viologen, as well as by cold stresses. These results showed that CaKR1 fusion with soluble, modified green fluorescent protein (smGFP) was localized to the cytosol in Arabidopsis protoplasts, suggesting that CaKR1 might be involved in responses to both biotic and abiotic stresses in pepper plants.

Virtual Screening for Potential Inhibitors of NS3 Protein of Zika Virus

  • Sahoo, Maheswata;Jena, Lingaraja;Daf, Sangeeta;Kumar, Satish
    • Genomics & Informatics
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    • v.14 no.3
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    • pp.104-111
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    • 2016
  • Zika virus (ZIKV) is a mosquito borne pathogen, belongs to Flaviviridae family having a positive-sense single-stranded RNA genome, currently known for causing large epidemics in Brazil. Its infection can cause microcephaly, a serious birth defect during pregnancy. The recent outbreak of ZIKV in February 2016 in Brazil realized it as a major health risk, demands an enhanced surveillance and a need to develop novel drugs against ZIKV. Amodiaquine, prochlorperazine, quinacrine, and berberine are few promising drugs approved by Food and Drug Administration against dengue virus which also belong to Flaviviridae family. In this study, we performed molecular docking analysis of these drugs against nonstructural 3 (NS3) protein of ZIKV. The protease activity of NS3 is necessary for viral replication and its prohibition could be considered as a strategy for treatment of ZIKV infection. Amongst these four drugs, berberine has shown highest binding affinity of -5.8 kcal/mol and it is binding around the active site region of the receptor. Based on the properties of berberine, more similar compounds were retrieved from ZINC database and a structure-based virtual screening was carried out by AutoDock Vina in PyRx 0.8. Best 10 novel drug-like compounds were identified and amongst them ZINC53047591 (2-(benzylsulfanyl)-3-cyclohexyl-3H-spiro[benzo[h]quinazoline-5,1'-cyclopentan]-4(6H)-one) was found to interact with NS3 protein with binding energy of -7.1 kcal/mol and formed H-bonds with Ser135 and Asn152 amino acid residues. Observations made in this study may extend an assuring platform for developing anti-viral competitive inhibitors against ZIKV infection.

Mathematical model for reactive transport of heavy metals in soil column: Based on PHREEQC and HP1 simulators

  • Tameh, Fatemeh Izadi;Asadollahfardi, Gholamreza;Darban, Ahmad Khodadadi
    • Advances in environmental research
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    • v.6 no.1
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    • pp.67-81
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    • 2017
  • Mining activities play a significant role in environmental pollution by producing large amounts of tailings which comprise heavy metals. The impressive increase in mining activities in recent decades, due to their high influence on the industry of developing countries, duplicates the need for a substantial effort to develop and apply efficient measures of pollution control, mitigation, and abatement. In this study, our objective was to investigate the effect of simulation of the leachate, pH and inflow intensity of transport of $Pb^{2+}$, $Zn^{2+}$, and $Cd^{2+}$ through Lakan lead and zinc plant tailings, in Iran, and to evaluate the modeling efficiency by comparing the modeling results and the results obtained from previous column studies. We used the HP1 model and the PHREEQC database to simulate metals transport through a saturated soil column during a 15 day time period. The simulations assumed local equilibrium. As expected, a lower pH and inflow intensity increased metal transport. The retardation of heavy metals followed the order $Zn^{2+}$ > $Pb^{2+}$ > $Cd^{2+}$ and the removal concentrations of Cd, Pb, and Zn at the inflow intensity critical scenario, and Cd and Pb at inflow acidity critical scenario exceeded the allowable EPA and Iranian's 1053 standard thresholds. However, although the simulation results generally agreed well with the results of the column study, improvements are expected by using multi-dimensional models and a kinetic modeling approach for the reactions involved. The results of such investigations will be highly useful for designing preventative strategies to control reactive transport of hazardous metals and minimize their environmental effects.

Reanalysis of 2007 Korean National Health and Nutrition Examination Survey (2007 KNHANES) Results by CAN-Pro 3.0 Nutrient Database (2007년도 국민건강영양조사 결과 재분석 : CAN-Pro 3.0 식품영양가표의 활용)

  • Shim, Youn-Jeong;Paik, Hee-Young
    • Journal of Nutrition and Health
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    • v.42 no.6
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    • pp.577-595
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    • 2009
  • This study aimed to reanalyze energy and nutrient intakes of 2007 Korean Nutrition and Health Examination Survey (KNHANES) using CAN-Pro 3.0, a commonly used nutrient analysis software in Korea. Food items and their codes were selected from 2007 KNHANES dietary intake file and converted to food codes of CAN-Pro 3.0 nutrient database (NDB). Of the 1,324 total food items, 1,155 items were converted by direct matching, 123 items were matched using other items in CAN-Pro 3.0 NDB and 42 items were matched using external sources. Consumption frequencies of items converted by direct matching contributed 94.5% of total consumption. Nutrient intakes of 4,091 participants of 2007 KNHANES, over 1 year old, were recalculated using CAN-Pro 3.0 NDB and compared with intakes in 2007 KNHANES dietary intake file. Intakes for energy and all nutrients except protein and Vitamin C calculated by two NDBs were significantly different by paired t-test (p < 0.001), but significantly correlated by Pearson' correlation coefficients (p < 0.001). Percent differences between the NDBs ranged from 0.3% to 15.1%, low for protein, energy, vitamin C, iron, vitamin B$_2$ (below 5%) but high for phosphorus, retinol, vitamin A, and $\beta$-carotene (over 10%). Age group, sex, and their interactions significantly influenced six nutrients (p < 0.05). Intake levels of zinc, vitamin B6, vitamin E, folate and cholesterol were not available in 2007 KNHANES but were calculated by CAN-Pro 3.0. Mean intake levels of zinc, vitamin B$_6$, vitamin E, and folate by age and sex groups revealed that some groups had mean levels below RI (Recommended Intake) or AI (Adequate Intake) levels. Intake level of cholesterol was higher than the recommended level (below 300 mg/day) in some groups, especially males. Results of the present study indicate the need for comparable and more comprehensive NDB to be used for dietary assessment of KNHANES and other researches. More rigorous evaluation of nutrients which have not been reported in KNHANES is needed.

Issues pertaining to Mg, Zn and Cu in the 2020 Dietary Reference Intakes for Koreans

  • Chung, Hae-Yun;Lee, Mi-Kyung;Kim, Wookyoung;Choi, Mi-Kyeong;Kim, Se-Hong;Kim, Eunmee;Kim, Mi-Hyun;Ha, Jung-Heun;Lee, Hongmie;Bae, Yun-Jung;Kwun, In-Sook
    • Nutrition Research and Practice
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    • v.16 no.sup1
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    • pp.113-125
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    • 2022
  • In the current years, it has now become necessary to establish standards for micronutrient intake based on scientific evidence. This review discusses issues related to the development of the 2020 Dietary Reference Intakes for Koreans (KDRI) for magnesium (Mg), zinc (Zn), and copper (Cu), and future research directions. Following issues were encountered when establishing the KDRI for these minerals. First, characteristics of Korean subjects need to be applied to estimate nutrient requirements. When calculating the estimated average requirement (EAR), the KDRI used the results of balance studies for Mg absorption and factorial analysis for Zn, which is defined as the minimum amount to offset endogenous losses for Zn and Mg. For Cu, a combination of indicators, such as depletion/repletion studies, were applied, wherein all reference values were based on data obtained from other countries. Second, there was a limitation in that it was difficult to determine whether reference values of Mg, Zn, and Cu intakes in the 2020 KDRI were achievable. This might be due to the lack of representative previous studies on intakes of these nutrients, and an insufficient database for Mg, Zn, and Cu contents in foods. This lack of database for mineral content in food poses a problem when evaluating the appropriateness of intake. Third, data was insufficient to assess the adequacy of Mg, Zn, and Cu intakes from supplements when calculating reference values, considering the rise in both demand and intake of mineral supplements. Mg is more likely to be consumed as a multi-nutrient supplement in combination with other minerals than as a single supplement. Moreover, Zn-Cu interactions in the body need to be considered when determining the reference intake values of Zn and Cu. It is recommended to discuss these issues present in the 2020 KDRI development for Mg, Zn, and Cu intakes in a systematic way, and to find relevant solutions.

Evaluation of the Nutritional Value of Traditional Korean Noodles through Energy Density and Diversity (에너지 밀도 및 다양성 측면의 전통 면류의 영양적 가치 평가에 관한 연구)

  • Yang, YoonKyoung;Kim, SungOk;Kim, Juhyeon
    • The Korean Journal of Food And Nutrition
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    • v.27 no.4
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    • pp.732-741
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    • 2014
  • Korean foods have the strengths to addresssome of the health problems of modern man. To assess the properties of Korean noodles, daily value %, DVS, DDS and energy density were compared between many kinds of noodles from around the world. Using a variety of reference materials, a nutritional database of noodles was built for this study. For carbohydrate, lipid, vitamin A, vitamin K, vitamin C, niacin, zinc, and copper, the daily values % of western noodles were significantly higher. Also, the serving size of Korean noodles was significantly small. Comparing the average energy density of the noodles, they showed $1.87{\pm}0.93kcal/g$ (Korean noodles), $2.42{\pm}1.08kcal/g$ (western noodles) and $1.84{\pm}0.84kcal/g$ (other noodles). The dietary fiber, polyphenols, and flavonoids content of the noodles showed no significant difference. Neither DVS nor DDS showed a statistically significant difference. In the Korean noodles, the GMDFV pattern showed a diverse choice of food groups. Korean noodles show a lower energy density, and the small serving size to have favorable for the prevention of obesity. Thus, Korean noodles are an excellent choice in terms of diversity and energy density.

Expression of Metallothionein mRNA in Cadmium Treated Leydig Cells (테스토스테론생성 레이디히세포(Leydig)에서의 메탈로치오닌 유전자 발현특성연구)

  • Park Kwangsik
    • Environmental Analysis Health and Toxicology
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    • v.19 no.3
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    • pp.261-269
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    • 2004
  • Although the biological functions of metallothioneins (MTs) are still being investigated, they have been suggested to be involved in detoxification of heavy metals, scavenging of free radicals, and protection against alkylating agents. MTs have been reported to be induced in most of animal tissues by heavy metals such as zinc, copper, mercury and cadmium, and the proteins have binding affinities to the metals. However, the presence or induction of MTs was reported not to be clear in leydig cells, which produce testosterone for the maturation of spermatozoa in male testes. In this study, we investigated the inducibility of metallothionein isomers by cadmium in cultured mouse leydig cells. Total RNA was extracted from the near confluent grown leydig cells and RT-PCR was Performed using the Primers which were synthesized on the basis of MT-1, 2, 3 and 4 cDNA from GenBank database. As results, MT-1 and MT-2 mRNA were found to be expressed in cadmium non-treated control cells and MT 1 mRNA expression was dose-dependent when leydig cells were treated with cadmium chloride. But MT-3 which is known to be brain specific and MT-4 which is another isoform of metallothionein, were not expressed. Other genes induced or depressed in cadmium treated leydig cells were also identified by microarray techniques.

CaWRKY2, a Chili Pepper Transcription Factor, Is Rapidly Induced by Incompatible Plant Pathogens

  • Oh, Sang-Keun;Yi, So Young;Yu, Seung Hun;Moon, Jae Sun;Park, Jeong Mee;Choi, Doil
    • Molecules and Cells
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    • v.22 no.1
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    • pp.58-64
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    • 2006
  • WRKY family proteins are a class of plant-specific transcription factors involved in stress response signaling pathways. In this study a gene encoding a putative WRKY protein was isolated from a pepper EST database (http://genepool.kribb.re.kr). The cDNA, named Capsicum annuum WRKY2 (CaWRKY2), encodes a putative polypeptide of 548 amino acids, containing two WRKY domains with zinc finger motifs and two potential nuclear localization signals. Northern blot analyses showed that CaWRKY2 mRNA was preferentially induced during incompatible interactions of pepper plants with PMMoV, Pseudomonas syringae pv. syringae 61, and Xanthomonas axonopodis pv. vesicatoria race 3. Furthermore, CaWRKY2 transcripts were strongly induced by wounding and ethephon treatment, whereas only moderate expression was detected following treatment with salicylic acid and jasmonic acid. CaWRKY2 was translocated to the nucleus when a CaWRKY2-smGFP fusion construct was expressed in onion epidermal cells. CaWRKY2 also had transcriptional activation activity in yeast. Taken together our data suggest that CaWRKY2 is a pathogen-inducible transcription factor that may have a role in early defense responses to biotic and abiotic stresses.