• Microbiology and Biotechnology Letters
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• v.20 no.1
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• pp.61-67
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• 1992

The synthesis of N-benzyloxycarbonyl-L-aspartyl-L-phenylalanine methyl ester(ZAPM), a precursor of aspartame, from N-benzyloxycarbonyl-L-aspartic acid(Z-Asp) and L-phenylalanine methyl ester hydrochloride(L-PM-HCl) was investigated in ethylacetate-MES buffer two-phase system using thermolysin. In organic two-phase system, the degree of spontaneous hydrolysis of L-PM. HCl was significantly reduced with increasing the volume ratio of organic to aqueous phase. Stability of thermolysin in organic two-phase system was found to be higher than that in MES buffer solution. More than 90% of initial enzyme activity was maintained after 10 days of incubation in case that the volume of organic phase was equal to that of buffer phase, while the half life of thermolysin was about 2 days in aqueous buffer solution. The results of partitioning of substrates and product in organic two-phase system showed that the difference in partition coefficients between substrates and product was maximum at pH 5.5. The optimal pH for 2-APM synthesis in organic two-phase system was found to be 5.5-5.8, which is consistent with the value expected from the partition experiments. As the concentration of substrates was increased the conversion yield of Z-APM was increased with concomitant reduction of L-PMqHC1 hydrolysis. In case that the concentration of L-PM-HCl and Z-Asp were 160 mM and 80 mM respectively, the conversion yield of Z-APM reached 90% after 28 hrs of reaction. The yield obtained at different volume ratio of organic phase compares well with the predicted equilibrium constant in biphasic system.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1053-1056
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• 2008

An application of CSG (Constructive Solid Geometry) modeling technique in Machining Simulation is introduced in this paper. The current CSG model is based on z-buffer CSG Rendering Algorithm. In order to build a CSG model, frame buffers of VGA (Video Graphic Accelerator) should be used in term of color buffer, depth buffer, and stencil buffer. In addition to using CSG model in machine simulation Stock and Cutter Swept Surface (CSS) should be solid. Method to create a solid Cuboid stock and Ball-end mill CSS are included in the present paper. Boolean operations are used to produce the after-cut part, especially the Difference operation between Stock and CSS as the cutter remove materials form stock. Finally, a small program called MaSim which simulates one simple cut using this method was created.

• Proceedings of the IEEK Conference
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• 2000.07b
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• pp.1088-1091
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• 2000

Superquadrics can represent various and complex 3D objects with only some parameters(size, position, deformation etc.). So if we use both superquadrics and deformed superquadrics, we can also represent more realistic 3D objects which are existed in real world. In this paper we use the z-buffer algorithm and stencil buffer together because this is very useful when the superquadric primitives are combined. The fundamental ideas are illustrated with a number of tables and figures.

• 한국신재생에너지학회:학술대회논문집
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• 2007.06a
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• pp.172-175
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• 2007

The Performance of a PEMFC stack is strongly dependent on the uniform reactants distribution on MEA. The uniform distribution can be achieved by flow-field pattern and manifold design optimized to satisfy operating conditions. This paper investigates uniform reactants distribution in channels by changing manifold shape and inlet mass flow rate. Typical U and Z shape and modified U and Z shape manifolds with buffer zone were designed. To check the uniform reactants distribution, standard deviation of mass flow rate was compared. The numerical results show that the inlet mass flow rate, inlet shape, and manifolds shape are critical factor for uniform distribution.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1993.10a
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• pp.423-427
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• 1993

An efficient 3-axis NC milling simulator is presented. The geometric simulation of milling is based on z-map structure and voxels. For the graphic simulation,dimetric projection is adopted. As a result, two faces of a voxel are exposed and they are overlapped. Visible faces are determined by using z-butter method. The simulation system is developed in IBM-PC compatible with satisfactory result.

• Proceedings of the Korea Multimedia Society Conference
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• 2002.11b
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• pp.497-502
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• 2002

레이 트레이싱이나 텍스쳐 맵핑에 의한 Implicit 곡면에서의 앤티 앨리어싱을 위한 방법으로 일반적으로 stochastic 샘플링 방법이 많이 사용되고 있다. 하지만 이 방법은 복잡한 함수로 표현된 Implicit 곡면에 더 많은 복잡한 계산을 요구하며 이에 따라 처리 시간과 비용이 많이 든다. 본 논문에서는 복셀(voxel)로 표현되는 Implicit 곡면에서 효율적인 방법 및 계산으로 앨리어싱을 감소시키기 위하여 shifted 더블 Z-버퍼를 이용하였으며, 또한 box-filter와 tent-filter를 적용함으로써 양질의 Implicit 곡면 이미지를 표현하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.4
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• pp.304-308
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• 2005

The response of IPTG induction was investigated through the monitoring of the alkali consumption rate and buffer capacity during the cultivation of recombinant E. coli BL21 (DE3) harboring the plasmid pRSET-LacZ under the control of lac promoter. The rate of alkali consumption increased along with cell growth, but declined suddenly after approximately 0.2 h of IPTG induction. The buffer capacity also declined after 0.9 h of IPTG induction. The profile of buffer capacity seems to correlate with the level of acetate production. The IPTG response was monitored only when introduced into the mid-exponential phase of bacterial cell growth. The minimum concentration of IPTG for induction, which was found out to be 0.1 mM, can also be monitored on-line and in-situ. Therefore, the on-line monitoring of alkali consumption rate and buffer capacity can be an indicator of the metabolic shift initiated by IPTG supplement, as well as for the physiological state of cell growth.

• Journal of Korea Multimedia Society
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• v.2 no.1
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• pp.80-88
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• 1999

In this paper, we propose a Special Memory for Graphics(SMGRA) which accelerates memory access time for graphics operations. The SMGRA has a rectangular array memory architecture which has already proposed by Whelan to process pixels in the rectangle area simultaneously, but the SMGRA should improve address decoding time and reduce the number of address pins by using address multiplexing scheme. The SMGRA has a Z-value comparator in the DRAM which is to convert read-modify-write Z buffer into single-write only operation that improves approximately 50% frame buffer access bandwidth.

• Korean Journal of Food Science and Technology
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• v.23 no.6
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• pp.695-701
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• 1991

Spent layer breast meat and leg meat samples washed with 0.05 M sodium bicarbonate solution and 0.04 M phosphate buffer(pH 8.3) showed decreases in heat denaturation temperature indicating the destabilization of myofibrillar proteins. The destabilization was attributed to the solubilization of 95 Kdalton and 55 kdalton proteins from the myofibrils observed in gel-electrophoretograms. Transmission electron microscopy further indicated the breakage of Z-lines.

• Proceedings of the KIEE Conference
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• 2000.11d
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• pp.775-777
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• 2000

본 논문에서는 buffer를 장착한 양 방향성 $a{\times}b$ switch들로 구성된 fat-tree network의 성능 분석 기법을 제안하고, 분석 모형의 타당성을 검증하였다. 제안한 분석 기법은 먼저 스위치 내부의 데이터 이동 패턴을 확률식으로 표현하고. 나아가서 buffer를 장착한 $a{\times}b$ switch의 buffer 크기에 따른 정상상태 throughput을 간단한 수식으로 구할 수 있도록 하였다. 이를 토대로 buffer를 장착한 $a{\times}b$ switch로 구성된 fat-tree network의 성능을 분석하고, 제안한 분석모형의 실효성 입증을 위하여 simulation을 시행한 후 결과를 비교 분석하였다.