• Journal of Microbiology and Biotechnology
• /
• 제22권4호
• /
• pp.501-509
• /
• 2012

A 990 bp full-length gene (xynAHJ2) encoding a 329-residue polypeptide (XynAHJ2) with a calculated mass of 38.4 kDa was cloned from Bacillus sp. HJ2 harbored in a saline soil. XynAHJ2 showed no signal peptide, distinct amino acid stretches of glycoside hydrolase (GH) family 10 intracellular endoxylanases, and the highest amino acid sequence identity of 65.3% with the identified GH 10 intracellular mesophilic endoxylanase iM-KRICT PX1-Ps from Paenibacillus sp. HPL-001 (ACJ06666). The recombinant enzyme (rXynAHJ2) was expressed in Escherichia coli and displayed the typical characteristics of low-temperature-active enzyme (exhibiting optimum activity at $35^{\circ}C$, 62% at $20^{\circ}C$, and 38% at $10^{\circ}C$; thermolability at ${\geq}45^{\circ}C$). Compared with the reported GH 10 low-temperature-active endoxylanases, which are all extracellular, rXynAHJ2 showed low amino acid sequence identities (<45%), low homology (different phylogenetic cluster), and difference of structure (decreased amount of total accessible surface area and exposed nonpolar accessible surface area). Compared with the reported GH 10 intracellular endoxylanases, which are all mesophilic and thermophilic, rXynAHJ2 has decreased numbers of arginine residues and salt bridges, and showed resistance to $Ni^{2+}$, $Ca^{2+}$, or EDTA at 10 mM final concentration. The above mechanism of structural adaptation for low-temperature activity of intracellular endoxylanase rXynAHJ2 is different from that of GH 10 extracellular low-temperature-active endoxylanases. This is the first report of the molecular and biochemical characterizations of a novel intracellular low-temperature-active xylanase.

• Parasites, Hosts and Diseases
• /
• 제58권2호
• /
• pp.153-159
• /
• 2020

The chigger mite Leptotrombidium sialkotense is one of the 6 main vectors of scrub typhus in China. Before present study, L. sialkotense was found in some parts of Hunan province, China with a narrow geographical distribution. During field investigation 2016-2017, we found L. sialkotense in Jingha, southern Yunnan, China. Of 15 small mammal host species, L. sialkotense were collected from 6 species of the hosts. Rattus brunneusculus was a dominant host of L. sialkotense, from which 98.3% of the mites were collected. The chigger mite showed a relatively high infestation prevalence (P_M =11.7%) and mean abundance (MA=0.5) in comparison with the rest 5 host species. These results reveal a certain host specificity of L. sialkotense to a rat R. brunneusculus. The mite L. sialkotense showed an aggregated distribution on the host (P<0.05). A positive correlation observed between L. sialkotense and the body length of hosts. There was a positive interspecific association between L. sialkotense and 2 other dominant vectors, L. deliense and L. scutellare.

• Parasites, Hosts and Diseases
• /
• 제59권6호
• /
• pp.625-634
• /
• 2021

Based on the field investigations in 91 investigation sites (counties) in southwest China between 2001 and 2019, the present paper reported the chigger mites on A. agrarius mice in southwest China for the first time by using a series of statistical methods. From 715 striped field mice captured in 28 of 91 investigated sites, only 255 chiggers were collected, and they were identified as 14 species, 6 genera in 3 subfamilies under 2 families. Of 715 A. agrarius mice, only 24 of them were infested with chigger mites with low overall prevalence (P_M=3.4%), overall mean abundance (M_A=0.36 mites/host) and overall mean intensity (M_I=10.63 mites/host). The species diversity and infestation of chiggers on A. agrarius were much lower than those previously reported on some other rodents in southwest China. On a certain species of rodent, A. agrarius mouse in southwest China seems to have a very low susceptibility to chigger infestations than in other geographical regions. Of 14 chigger species, there were 3 dominant species, Leptotrombidium sialkotense, L. rupestre and Schoengastiella novoconfuciana, which were of aggregated distribution among different individuals of A. agrarius hosts. L. sialkotense, one of 6 main vectors of scrub typhus in China, was the first dominant on A. agrarius. The species similarity of chigger mites on male and female hosts was low with C_SS=0.25, and this reflects the sex-bias of different genders of A. agrarius mice in harboring different chigger species.

• Parasites, Hosts and Diseases
• /
• 제61권3호
• /
• pp.272-281
• /
• 2023

Chigger mites are the vector of scrub typhus. This study estimates the infestation status and ecological characteristics of chiggers on the chestnut white-bellied rat Niviventer fulvescens in Southwest China between 2001 and 2019. Chiggers were identified under the microscope, and infestation indices were calculated. The Preston's log-normal model was used to fit the curve of species abundance distribution. A total of 6,557 chiggers were collected in 136 of 342 N. fulvescens rats, showing high overall infestation indices (prevalence=39.8%, mean abundance=19.2, mean intensity=48.2) and high species diversity (S=100, H'=3.0). Leptotrombidium cangjiangense, Neotrombicula japonica, and Ascoschoengastia sifanga were the three dominant chigger species (constituent ratio=42.9%; 2,736/6,384) and exhibited an aggregated distribution among different rat individuals. We identified 100 chigger species, with 3 of them (Leptotrombidium scutellare, Leptotrombidium wenense, and Leptotrombidium deliense) as the main vectors of scrub typhus in China and nine species as potential vectors of this disease. Disease vector occurrence on N. fulvescens may increase the risk of spreading scrub typhus from rats to humans. Chigger infestation on N. fulvescens varied significantly in different environments. The species abundance distribution showed a log-normal distribution pattern. The estimated number of chigger species on N. fulvescens was 126 species.

• 천문학회지
• /
• 제47권3호
• /
• pp.105-113
• /
• 2014

We apply differential affine velocity estimator (DAVE) to the Solar Dynamics Observatory (SDO)/Helioseismic and Magnetic Imager (HMI) 12-min line-of-sight magnetograms, and separately calculate the injected magnetic helicity for the leading and the following polarities of nine emerging bipolar active regions (ARs). Comparing magnetic helicity flux of the leading polarity with the following polarity, we find that six ARs studied in this paper have the following polarity that injected more magnetic helicity flux than that of the leading polarity. We also measure the mean area of each polarity in all the nine ARs, and find that the compact polarity tend to possess more magnetic helicity flux than the fragmented one. Our results confirm the previous studies on asymmetry of magnetic helicity that emerging bipolar ARs have a polarity preference in injecting magnetic helicity. Based on the changes of unsigned magnetic flux, we divide the emergence process into two evolutionary stages: (1) an increasing stage before the peak flux and (2) a constant or decreasing stage after the peak flux. Obvious changes on magnetic helicity flux can be seen during transition from one stage to another. Seven ARs have one or both polarity that changed the sign of magnetic helicity flux. Additionally, the prevailing polarity of the two ARs, which injects more magnetic helicity, changes form the following polarity to the leading one.

• Journal of Information Processing Systems
• /
• 제15권6호
• /
• pp.1365-1377
• /
• 2019

Summarizing the differences in Chinese-Vietnamese bilingual news plays an important supporting role in the comparative analysis of news views between China and Vietnam. Aiming at cross-language problems in the analysis of the differences between Chinese and Vietnamese bilingual news, we propose a new method of summarizing the differences based on an undirected graph model. The method extracts elements to represent the sentences, and builds a bridge between different languages based on Wikipedia's multilingual concept description page. Firstly, we calculate the similarity between Chinese and Vietnamese news sentences, and filter the bilingual sentences accordingly. Then we use the filtered sentences as nodes and the similarity grade as the weight of the edge to construct an undirected graph model. Finally, combining the random walk algorithm, the weight of the node is calculated according to the weight of the edge, and sentences with highest weight can be extracted as the difference summary. The experiment results show that our proposed approach achieved the highest score of 0.1837 on the annotated test set, which outperforms the state-of-the-art summarization models.

• 복식
• /
• 제60권9호
• /
• pp.41-57
• /
• 2010

Yunnan, where twenty-six minor ethnics live, is located in Southwest part of China. They have preserved and developed their folk costume, artcraft, music and dance in their peculiar way with natural and cultural surroundings. So, Yunnan is known as a living folk museum. It's worth examining how climate and circumstances change has affected certain differences of dress shape in same ethnic. Research into costumes of minor ethnics can contribute to the study of origin of the trousers, as this group of people have well succeeded the basic form of trousers through their cultural history and everyday life. This study, at first, investigates geographical surroundings of each minor ethnic group, and figures out the style of all trousers by measuring the each size, then compares design color pattern and fabric. The origin of trousers examined by formative characteristics has been classified and comparatively studied. The study materials are twenty-three trousers selected from in Yunnan Folk Museum as well as other relevant literature records. This study suggests that one can examine the type of human culture through the forms of costume which reflects folk custom geography economy etc. This, in turn, can be used as a basic information for other studies, and in particular, for the study of origin and structure of the trousers.

• Journal of Microbiology and Biotechnology
• /
• 제31권6호
• /
• pp.840-846
• /
• 2021

To improve the bile salt and acid tolerance of probiotics against gastrointestinal stresses, we investigated the effects of soybean lecithin and whey protein concentrate (WPC) 80 on the bile salt tolerance of Lacticaseibacillus paracasei L9 using a single-factor methodology, which was optimized using response surface methodology (RSM). The survival rate of L. paracasei L9 treated with 0.3% (w/v) bile salt for 2.5 h, and combined with soybean lecithin or WPC 80, was lower than 1%. After optimization, the survival rate of L. paracasei L9 incubated in 0.3% bile salt for 2.5 h reached 52.5% at a ratio of 0.74% soybean lecithin and 2.54% WPC 80. Moreover, this optimized method improved the survival rate of L. paracasei L9 in low pH condition and can be applied to other lactic acid bacteria (LAB) strains. Conclusively, the combination of soybean lecithin and WPC 80 significantly improved the bile salt and acid tolerance of LAB. Our study provides a novel approach for enhancing the gastrointestinal tolerance of LAB by combining food-derived components that have different properties.

• Journal of Animal Science and Technology
• /
• 제64권1호
• /
• pp.183-186
• /
• 2022

Lactiplantibacillus plantarum (L. plantarum) ST was isolated from De'ang pickled tea in Yunnan Province, China. The genomes of strain ST were fully sequenced and analyzed using the PacBio RS II sequencing system. Our previous study has shown that L. plantarum ST is a potential probiotic strain. It had strong tolerance in the simulated artificial gastrointestinal tract, and in the antagonism tests, this strain showed strong antibacterial activity. Therefore, as a probiotic, it may be used in animal breeding. L. plantarum ST genome was composed of 1 circular chromosome and 7 plasmids. The length of the whole genome was 3320817 bp, and the annular chromosome size was 3058984 bp, guanine + cytosine (G ± C) content (%) was 44.76%, which contained 2945 protein-coding sequences (CDS). This study will contribute to a further comprehensive understanding of L. Plantarum ST at the genomic level and provide a theoretical basis for its future application in animal breeding.

• Journal of Information Processing Systems
• /
• 제18권4호
• /
• pp.535-548
• /
• 2022

Using a rich resource language to classify sentiments in a language with few resources is a popular subject of research in natural language processing. Burmese is a low-resource language. In light of the scarcity of labeled training data for sentiment classification in Burmese, in this study, we propose a method of transfer learning for sentiment analysis of a language that uses the feature transfer technique on sentiments in English. This method generates a cross-language word-embedding representation of Burmese vocabulary to map Burmese text to the semantic space of English text. A model to classify sentiments in English is then pre-trained using a convolutional neural network and an attention mechanism, where the network shares the model for sentiment analysis of English. The parameters of the network layer are used to learn the cross-language features of the sentiments, which are then transferred to the model to classify sentiments in Burmese. Finally, the model was tuned using the labeled Burmese data. The results of the experiments show that the proposed method can significantly improve the classification of sentiments in Burmese compared to a model trained using only a Burmese corpus.