• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.484-493
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• 2012

BolA protein homologs are widely distributed in nature. In this report, we have studied for the first time YrbA, the only BolA homolog present in Escherichia coli, which we have renamed ibaG. We have constructed single and multiple ibaG mutants, and overexpressed ibaG in wild-type strains, in order to characterize this gene. The ibaG phenotypes are different from the bolA-associated round morphologies or growth profiles. Interestingly, ibaG and bolA single-and double-deletion mutants grow faster and have higher viabilities in rich media, whereas the overexpressed strains are significantly growth impaired. However, the mutant strains have lower viabilities than the wild type in the late stationary phase, indicating that both bolA and ibaG are important for survival in difficult growth conditions. bolA, as a transcription factor, binds to some promoters, but ibaG does not interact with the same DNA regions. We have determined that ibaG is transcribed in an operon with the murA gene, involved in the synthesis of peptidoglycan precursors. ibaG was also seen to change its mRNA expression pattern in response to acidic stress. ibaG may thus represent a new gene involved in cell resistance against acid stress.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.6
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• pp.597-602
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• 2015

CrMo alloy steel was nitrided using two types of processing methods, ion-nitriding processing and nitrocarburizing. Both processes were conducted for a duration of 30 min. To compare the surface hardness of the alloys created by the different processes, microhardness tests were conducted, and fatigue tests of each material were performed by a cantilever rotary bending fatigue test machine (Yamamoto, YRB 200) in the very high cycle regime ($N>10^7cycle$). Fractography of the fractured surfaces was conducted by scanning electron microscopy - to observe the fracture mechanisms of very high cycle fatigue and the effect of the nitriding process on the fatigue characteristics.