• 한국작물학회지
• /
• 제50권5호
• /
• pp.325-331
• /
• 2005

Seedlings of two rice genotyopes, cvs. Ilpumbyeo and Gancheokbyeo, were exposed to 0, 50 and 100 mM NaCl in nutrient solution for nine days. Plants were collected at the interval of 3 days and organic and inorganic solutes in leaves and roots and antioxidative enzyme activity in leaves were determined. Under salinity, the accumulation of soluble sugars occurred considerably in the older leaves of stressed seedlings compared to younger leaves and roots. The endogenous Na+ contents markedly increased at higher NaCl concentration in leaves and roots of seedlings, though it was higher accumulated in roots. Salinity resulted in an excessive proline accumulation in the stressed plants. A more pronounced increase was observed in Gancheokbyeo leaves. SOD activity in Impumbyeo cannot found any remarkable change, whereas, in Gancheokbyeo, its activity was rapidly decreased. CAT and POD activities increased with an increase in NaCl concentration in both genotypes. In sum­mary, the high capacity of rice seedlings to overcome an unfavorable growth condition such salt stress appears to be related to an adequate partition of organic solutes between shoots and roots and to changes in absorption, transport and re-translocation of salts.

• 한국농림기상학회지
• /
• 제7권3호
• /
• pp.220-226
• /
• 2005

오존 노출이 버즘나무의 광합성 특성에 미치는 영향을 조사하기 위하여, 2년생 버즘나무 유묘에 하루에 8시간씩 100 ppb의 오존을 처리하였다. 오존 처리를 진행하는 동안, 3주마다 버즘나무의 엽내 엽록소 함량과 광합성 특성을 측정하였다. 오존에 의한 잎의 가시적 피해는 나타나지 않았으며, 또한 엽내 엽록소 함량은 대조구와 뚜렷한 차이를 보이지 않았다. 그러나 광합성량, 기공전도도, 수분이용효율은 오존 처리 6주 후에 대조구보다 감소하였다. 한편 탄소고정효율과 광화학 효율은 오존 처리 3주와 6주 후에서 감소한 것으로 나타났다. 위의 결과를 토대로 볼 때, 탄소고정효율은 오존 스트레스에 가장 민감한 파라미터로 나타났으며, 이것은 오존 민감성을 평가하기 위한 매우 적당한 지표로 생각되었다.

• 농업과학연구
• /
• 제46권4호
• /
• pp.781-790
• /
• 2019

Changes in the physical and chemical properties of soil materials during the nursing process have a great influence on the quality of containerized seedlings and on growth and survival after planting. In this study, the effect of biomaterials and their mixed ratios on the growth of Zelkova serrata seedlings in a containerized seedling production system was investigated. Mushroom sawdust, pine bark, and carbonized rice husk were used as biomaterials. The mixed ratios were 10% and 20% volume ratio of the growing medium volume, including the untreated controls. There was no significant difference in the height growth of the Zelkova serrata seedlings according to the biomaterials. The root collar diameter was the highest with the 20% carbonized rice husk and the lowest with the mushroom sawdust. The difference between the highest quality index and the lowest quality index was 30% in the order of the carbonized rice husk, pine bark, control, and mushroom sawdust, but there was no statistical significance. In this study, if the growing medium mixed with biomaterials does not reduce the seedling growth compared with the control, it is considered that the biomaterial can replace a part of the growing media. Therefore, the results show that some of the growing media can be replaced with carbonized rice husk or pine bark when producing Zelkova serrata seedlings.

• 한국잡초학회지
• /
• 제15권4호
• /
• pp.247-253
• /
• 1995

The herbicide butachlor [N-(butoxymethyl)-2-chloro-N-(2,6-di-methylphenyl) acetamide] is widely used by farmers as a tool for weed management of transplanted rice(Oryza sativa L.) in Taiwan. The herbicide did not stop germination of rice and weed seeds, but strongly inhibited the subsequent growth of young shoots and roots. The inhibition was also strong on established seedlings. However, they could recover to normal growth after the herbicide effect disappeared. Butachlor greatly decreased the endogenous indole-3-acetic acid (IAA) but increased the endogenous abscisic acid (ABA) contents of rice seedlings. Addition of lAA into growth medium (Hoagland's solution) partly relieved growth inhibition. Pretreatment of both gibberellic acid ($GA_3$) and IAA 24 hours before butachlor treatment almost completely alleviated the butachlor-interfere with GA and/or IAA metabolism or their action resulting in the growth inhibition of rice. Butachlor was readily absorbed by rice roots. During 24 hours of uptake experiment, 32% of the applied herbicide was absorbed. Pretreatment of the herbicide for 2 days did ncx affect the absorption. Of the absorbed herbicide, 80% remained in roots, only 20% transported into shoots, and more than 50% was metabolized to water soluble substances. Thin-layer chromatographic (TLC) analysis indicated that the Rf value of the most abundant metabolite was butachlor-glutathione conjugate. Rice, barnyardgrass (Echinochloa crus-galli (L.) Beauv.), and monochoria (Monochoria vaginalis Presl) seedlings contained relatively high level of non-protein thiols, while the glutathione S-transferase (GST) activity was found highest in rice, barnyardgrass the next, monochoria the lowest. The difference in GST activity among these species might be related to their sensitivity to butachlor.

• 한국농림기상학회지
• /
• 제4권1호
• /
• pp.12-17
• /
• 2002

여수산업단지 같은 대기오염지역에서 채취한 사방오리나무의 질소고정 뿌리혹의 접종 효과를 보기 위해서 오염에 대해서 저항성 개체와 민감한 개체를 선발하고 종자를 채취한 후 파종을 하고 발아한 후 3주 후의 유묘를 같은 지역에서 채취한 뿌리혹으로 12개의 조합으로 접종을 하였다. 접종효과를 비교하기 위해서 광합성 능력을 측정하였다. 대기오염에 대해 저항성을 보이는 모수에서 채취한 종자가 민감한 개체에서 채취한 것보다 광합성 능력이 높은 경향을 나타냈다. 대기오염에 민감한 모수에서 채취한 뿌리혹으로 접종한 조합이 일반적으로 생리활성이 낮았다. 그러나 접종효과는 뿌리혹을 채취한 모수보다는 종자를 채취한 모수가 어떤 상태인가에 따라서 광합성 능력이 더 영향을 많이 받는 것으로 나타났다. 그렇기 때문에 생리적인 특성에 대한 영향은 뿌리혹을 채취한 모수보다는 종자의 모수가 더 중요한 요인 같다.

• 한국환경생태학회지
• /
• 제37권1호
• /
• pp.13-34
• /
• 2023

Many ecologists try to understand plant communities and societies' changing processes by interacting with environmental factors. Plant communities formed in the sidings of streams, which are more dynamic than forest vegetation, are closely related to the disturbance regime of streams. U. pumila, which forms communities at the edges of streams, is distributed over the central and northern regions of the Korean Peninsula. In this study, we intended to explain the regeneration of U. pumila communities in the upstream region of the Namhangang River. Heights from the stream surface to the mid-high points of communities increased when the age class of U. pumila communities increased. On the other hand, the height of U. pumila tended to increase and decrease as the age class increased. The richness (S), evenness (J'), diversity (H'), and maximum diversity (H'max) also showed a tendency to increase and then decrease as the age class increased. On the other hand, the number of stems per individual and density (D) decreased as the age class increased. Seedlings of U. pumila failed to regenerate in the communities at the intermediate or higher stage of the age class. Regeneration of seedlings occurred in the spaces formed by the flooding, which occurred periodically in streams. After regeneration, U. pumila communities grew like a single organism. On the other hand, the plant species consisting of U. pumila communities exhibited a process in which the S and H' increased and then decreased as they developed. In other words, we determined that the changes in the species compositions were associated with changes in each species' environment and community. A space where seeds can regenerate seedlings should be provided for the regeneration of U. pumila communities. In the mid/upstream region of the Namhangang River, where the present study focused, newly formed communities were rare because the space required to regenerate U. pumila ceased to exist. Accordingly, we suggest securing an appropriate space for the regeneration of seedlings to maintain the U. pumila communities in the upstream region of the Namhangang River.

• 생물환경조절학회지
• /
• 제10권3호
• /
• pp.159-164
• /
• 2001

겨울철 시설내에서 정전 등으로 인하여 수시간 동안 작물체가 생육한계 온도로 경과시 이오묘에 나타나는 피해와 피해발생의 생리적 요인 및 경감대책을 구명하기 위하여 저온 스트레스가 이이묘의 광합성 및 생리반응에 미치는 영향을 연구한 결과는 다음과 같다. 냉온처리($0^{\circ}C$)는 무처리에 비한 오이묘의 광합성량이 10시간 처리는 52.8%, 24시간 처리에서는 67.6% 감소시켰으나, 5시간처리는 무처리와 큰 차이가 없었다. 냉온처리 기간중의(3$^{\circ}C$, 15시간)광조사는 암상태에서 보다 광합성을 크게 저해시켰다. 냉온처리 후 회복기간 중에 암조건을 주면 광합성량이 다소 회복되었으나 광조사시에는 회복되지 못하였다. 0~6$^{\circ}C$ 범위내의 냉온처리는 무처리에 비해 뿌리활력을 유의하게 감소시켰고, 냉온처리 40일후 채취된 오이줄기의 일비액량은 처리간 유의차를 보이지 않았다. 냉온처리(3$^{\circ}C$)시 전당 함량은 무처리에 비해 24시간 처리에서는 12%, 48시간 처리에서는 23% 증가되었다. 한편 냉온처리에 의하여, 불포화지방산인 linolenic acid와 oleic acid는 증가되었으나, 포화지방산인 palmitic acid는 감소되었다.

• Journal of Ecology and Environment
• /
• 제41권6호
• /
• pp.152-164
• /
• 2017

Background: Natural regeneration of seedlings as well as saplings of Korean fir has been significantly impacted by the browsing from the early stages of their development, potentially, by roe deer for the last two to three decades at the study site since late 1980s. This study was carried out to investigate current status of the disturbance in the seedling development of Korean fir (Abies koreana) on Mt. Hallasan, Jeju Island, Korea. Methods: Field survey was carried out during June and August in 2016 to measure the characteristics of study site and understory vegetation by applying systematic sampling to 125 plots of $5m{\times}5m$ quadrat located on eastern slope of the mountain. Correlation and regression analyses were applied to the variables quantified from the data sets using the SAS software. Results: No saplings with their diameters at breast heights smaller than 5.0 cm were found at the study site indicating the serious disturbance in the natural regeneration of Korean fir at the study site. No seedlings with their heights taller than 36.0 cm were found at the study site indicating even more serious disturbance during earlier stage of the natural regeneration of Korean fir at the study site. A total of 616 individuals of the seedlings of Korean fir were found at 54 out of 125 sampling plots. One hundred thirty-eight seedlings (22.4%) out of 616 individual seedlings have the vestiges for being grazed, potentially, by roe deer. Conclusions: Due considerations should be given to the effects of browsing of the seedlings by roe deer to promote the natural regeneration of Korean fir, ultimately to restore Korean fir. It is needed for the managers of the forest to install fences around the forest area. Exclosure experiments as well as enclosure experiments of different densities of browsing should be carried out. In addition, treatment with different densities of Jeju dwarf bamboo should also be included in the experiment on Mt. Hallasan National Park, Jeju Island, Korea.

• 한국식품저장유통학회지
• /
• 제10권3호
• /
• pp.365-369
• /
• 2003

배추의 품종별 종자 및 성장 시기에 따른 3-butenyl isothiocyanate 및 total glucosinolates를 분석하기 위하여 동풍배추(Winter Pride) 및 55일 배추(55 Days) 품종의 종자, 발아 1일, 3일, 5일, 7일 된 어린 배추를 떡잎(cotyledon), 배축(hypocotyl), 뿌리(root) 부분으로 나누고 anion-exchange column(Dowex 1${\times}$2-100)을 통과시키고 아세톤 침전법을 사용하여 제조한 crude myrosinase로 반응시켜 함량 변화를 조사하였다. 종자에서는 55일 배추의 3-butenyl isothiocyanate의 함량이 동풍 배추 종자보다 다소 높았다. 55일 배추의 3-butenyl isothiocyanate 함량은 3-day-old seedlings의 떡잎과 배축에서 가장 높았으며 동풍 배추의 떡잎에서는 3-day-old seedlings까지 함량이 증가한 후 감소하였으며 배축은 3-day-old seedlings까지 함량이 증가하였다. Total glucosinolates를 분석한 결과 종자에서는 동풍 배추보다 35일 배추의 함량이 다소 높게 조사되었으며 55일 배추의 떡잎은 3-day-old seedlings에서 가장 높은 함량을 보였다. 배축 부분은 7-day-old seedlings까지 증가하였으며 뿌리 부분의 glucosinolates는 성장 기간동안 점차 감소하는 경향을 나타내었다. 동풍 배추의 떡잎과 배축 부분은 -day-old seedlings까지 증가하다가 감소하였다.

• BMB Reports
• /
• 제38권6호
• /
• pp.650-660
• /
• 2005

Proteins accumulated in dry, stratified Arabidopsis seeds or young seedlings, totaled 1100 to 1300 depending on the time of sampling, were analyzed by using immobilized pH gradient 2-DE gel electrophoresis. The molecular identities of 437 polypeptides, encoded by 355 independent genes, were determined by MALDI-TOF or TOF-TOF mass spectrometry. In the sum, 293 were present at all stages and 95 were accumulated during the time of radicle protrusion while another 18 appeared in later stages. Further analysis showed that 226 of the identified polypeptides could be located in different metabolic pathways. Proteins involved in carbohydrate, energy and amino acid metabolism constituted to about 1/4, and those involved in metabolism of vitamins and cofactors constituted for about 3% of the total signal intensity in gels prepared from 72 h seedlings. Enzymes related to genetic information processing increased very quickly during early imbibition and reached highest level around 30 h of germination.