• Journal of Korean Society of Forest Science
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• v.90 no.4
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• pp.565-572
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• 2001

The spatial genetic structure of Needle fir(Abies holophylla Max.) seedlings on forest gap within a Needle fir forest at Mt. Odae in Korea was analyzed on the basis of ISSR(inter-simple sequence repeats) marker analysis. The gap size was $1,500m^2(50m{\times}30m)$, and we sampled 416 one- or two-year-old seedlings by 2m intervals. Some trees at the upper crown layer except Needle firs and all trees at the middle and lower crown layers were removed, and Needle firs at the upper crown layer showed very weak growth strength or to be withering to death. The results of spatial autocorrelation using 31 polymorphic ISSR markers revealed that it was genetically homogeneous within spatial distance of 15.6m and the randomness of genetic distribution was from 15.6m to 31.2m. The genetic patch size of seedlings in forest gap might be restricted by the density of mother trees, making allow for the average height of adult Needle firs, the seed dispersal area, and the average distance between adults. For the directionality of seedling distribution, we investigated the variography using 'genetic configuration' which was the value of configuration in Multidimensional Scaling by genetic distance. In directional variogram, the increment of spatial distance from East to West direction was inversely proportional to genetic homogeneity. We presumed that this anisotrophy of seedling distribution at this forest gap resulted from the directionality of seed dispersal rather than the difference of fecundity between mother trees or the microhabitat variation, taking the evenness of forest floor condition, a vast seed production and the random distribution of seedlings at the studied site into consideration.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.3
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• pp.341-346
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• 1987

Seven barley varieties were tested under three different vernalization durations for observing the effects of vernalization duration and different growth habit on shoot apex development and heading date. The final leaf number per main stem in 3 and 6 weeks vernalized seedlings did not vary among varieties, but ranged 7 to 14 leaves in non-vernalized seedlings. The winter types had more leaves than the spring types. Days for each leaf emergence in non-vernalization were retarded 1. 3 to 1. 5 days in comparison with 3 or 6 weeks vernalized seedlings. In general, the leaf emergence speed of spring types was faster than that of winter types. The VI stage whose double ridge formed, did not vary in 6 weeks vernalization, but spring (Gangbori & Dongbori 2) and facultative or winter types showed two conspicuous difference patterns. The differences of days to X stage were great among different vernalization duration and varieties; the stage of spring types was reached faster than that of winter types. The early varieties within the same growth habit were reached to X stage faster, and the time of flag leaf emergence showed the similar tendency to the differentiation of X stage. The time of the first rapid stem internode elongation became late as for incompletely vernalized seedlings. The time within the same vernalization duration became later in winter types than in spring types, and even within the same growth habit, the time of early varieties became faster than that of late varieties. The growth habit in especially non-vernalized seedlings had highly significant correlation coefficients with the times of leaf development speed, leaf number per main stem, the first rapid stem internode and young spike elongations, X stage, and flag leaf emergence. However, the relationship between growth habit and time of heading in the field was not close.

• Journal of Korean Society of Forest Science
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• v.102 no.1
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• pp.66-73
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• 2013

This study was conducted to determine the effects of rice hull cover on seed germination and tray types, soil types, shading conditions for seedling growth of Codonopsis pilosuala. We also examined the feasibility of cultivation of small seedlings transplanted in the Gyeonggi-do area for a month. Under control condition, the seed germination was 8% whereas it dramatically increased to 78% under rice hull cover treatment. Under the different conditions (tray types, soil types, and shading conditions), young seedlings showed the best quality without shading in TKS soil of 50 plug cell tray, with the growth characteristics of plant height (11.9 cm), number of leaves (71), leaf width (3.1 cm), leaf length (2.6 cm), and root length (14.3 cm). Seedling quality was the best without any shading in TKS+perlite, with the physiological characteristics of evaporation (3.9 $mmol{\cdot}m^{-2}s^{-1}$), carbon assimilation (9.1 ${\mu}mol{\cdot}m^{-2}s^{-1}$), and water use efficiency (2.2 ${\mu}mol{\cdot}m^{-2}s^{-1}$). Considering the economical, morphological, physiological and survival rate of the seedlings, it was an ideal method for transplanting seedlings in the field after they have been grown for 30-45 days in TKS and TKS+perlite of 200 plug cell tray in 0% or 30% shaded conditions. As the results of 5 months examination on the possibility to cultivate Codonopsis pilosuala in the Gyeonggi-do area, 88% to 96% of survival rate was observed with normal induced flowers. Therefore, cultivation of Codonopsis pilosuala was possible in the Gyeonggi-do area. While there were symptoms of etiolation and wilting under no shading condition, they did not appear in 30% and 70% shading conditions. Therefore, cultivation of Codonopsis pilosuala is considered to be necessary 30% or 70% shading.

• Journal of Korean Society of Forest Science
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• v.94 no.6
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• pp.402-409
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• 2005

This study was undertaken to compare and estimate the severity of pitch canker of individual trees of Pinus rigida and Pinus rigida ${\times}$ P. taeda in two seed orchards in Jeju island, in which the orchards have been damaged by the pitch canker for seven years. Wind-pollinated two-year-old seedlings of P. rigida and P. rigida ${\times}$ P. taeda, in which the seedlings of P. rigida ${\times}$ P. taeda were from seeds of phenotypically selected, uninfected(but untested) trees, were inoculated with the pathogenic fungus, Fusarium circinatum, isolated from P. rigida and P. thunbergii. The virulence of the isolates was also identified. Statistically significant difference was found in 'stem cankers'(SC; ${\chi}^2=7.76$, P=0.05) among 4 plantations of P. rigida ${\times}$ P. taeda of two seed orchards. P. rigida was higher in 'top kill' (TK) and 'branch tip symptoms' (BT) than those of P. rigida ${\times}$ P. taeda. In artificial inoculation tests, mortality of the seedlings from the resistant candidates was 14% higher than that of the seedlings from the susceptible candidates. This result may becaused by unknown pollen trees and/or candidate tree selection based only on phenotype. Two of five fungal isolates, C-6-L(9) and C-6-L(19), showed significantly higher mortality (68% and 60%, respectively) than others, suggesting that these isolates can be used as virulent isolates for a mass artificial inoculation. Resistance candidate seedlings that were selected from this study can be utilized as useful materials for fundamental studies of genetics and biochemistry to breed resistance varieties to pitch canker.

• Journal of Bio-Environment Control
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• v.27 no.4
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• pp.302-311
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• 2018

Using cylindrical paper pot nursery method, three kinds of commercial tomatoes 'Dafnis', 'DOTAERANG DIA' and 'Maescala' were grafted onto a commercial rootstock 'B blocking'. From 10 to 40 days after graft-take, growth traits of seedlings were investigated by 0.5, 1.0 and 2.0S treatments of standard nutrient solution(S) for seedling growth, and top to root ratio(TRR), compactness(CP) and Dickson Quality Index(DQI) were calculated. Two weeks after transplanting of the seedlings under three different night temperature targeting to 10, 15, and $25^{\circ}C$, which were not precisely controlled, the relative growth rate (RGR) was investigated. The quantitative growth traits of grafted seedlings increased with increasing fertilizer concentration, and various range of seedling size could be produced. Compactness and DQI were significantly regressed (Adj $R^2=0.9480$). Short-term RGR after transplanting was higher at 1.0S treatment of standard nutrient solution at the seedling age of 30 days and 40 days after graft-take(DAGT). DQI and RGR were significantly regressed linearly at respective fertigation strength. Specially the diminishing slope of RGR was lower at 1.0S fertigation strength with the increase of DQI than others. The results indicate that DQI could be applied as a quality index of grafted tomato seedlings and the relation analysis between DQI and short-term RGR also could be used to determine the good quality seedlings of grafted tomato grown in cylindrical paper pot.

• Journal of Korean Society of Forest Science
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• v.73 no.1
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• pp.43-54
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• 1986

This study was carried out to investigate the influence of light intensity on the growth of Korean white pine (Pinus koraiensis) seedlings. The seedlings were grown under four different relative light intensities: 100%, 63%, 37%, and 19% of full sunlight by covering with saran screen on seed bed. The results obtained were summarized as follows; 1. The highest shoot elongation was found at 37% in relative light intensity (RLI) plot, and the best growth in root, seedling length and diameter increment appeared at 100% on RLI plot. Contribution rates of shading to growth in shoot elongation, root growth, seedling length, and diameter increment were 9.3%, 26.2%, 21.0%, and 48.7%, respectively. 2. The greatest number of one-year-old needle fascicle was found at 100% in RLI plot, whereas that of two-year-old needle fascicle appeared at 37% in RLI plot. The contribution rate of the light intensity to number of needle fascicle was 3.9% and factor of leaf age contributed to number of needle fascicle in 27.8%. The length of needles grown under different light intensities varied with needle ages. The longest length of new needles appeared at 37% in RLI plot, but old needles were not clear in influence of light intensity. The contribution rate to needle length by the light intensity was 2.5%. 3. The heaviest value of the fresh and dry weight of seedlings appeared at 100% of RLI plot then decreased with light intensity. The contribution rates to fresh and dry weight by the shading were 38% and 7.6% respectively. 4. The largest value of the dry weight of needles appeared at 100% of RLI plot and then decreased with light intensity. The contribution rates to dry weight of needles by the light intensity was 13.18%. 5. The values of T/R ratio increased with decreased light intensity and the contribution rate was 7.0%. 6. Positive correlation and linear regressions were recognized between dry weight of leaf and other factors (dry weight of shoot, root, seedling and diameter increment).

• Journal of Korean Society of Forest Science
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• v.113 no.1
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• pp.88-96
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• 2024

This study was conducted to determine the optimal light conditions for the in situ and ex situ conservation and restoration of Osmanthus insularis, a rare plant species in South Korea. Evaluations included the growth performance, leaf morphological features, photosynthetic characteristics, and photosynthetic pigment contents of seedlings grown from April to November under different light conditions (100%, 55%, 20%, and 10% relative light intensity). The shoot lengths and root collar diameters did not differ significantly with relative light intensity. The dry weights of leaves, stems, and roots and the leaf number were highest at 55% relative light intensity. The leaf shape showed morphological acclimation to light intensity, with leaf area decreasing and thickness increasing as the relative light intensity increased. Several leaf parameters, including photosynthetic rate and stomatal conductance at light saturation point, net apparent quantum yield, and dark respiration, as well as chlorophyll a, chlorophyll b, and carotenoid contents, were all highest at 55% relative light intensity. Under full light conditions, the leaves were the smallest and thickest, but the chlorophyll content was lower than at 55% relative light intensity, resulting in lower photosynthetic ability. Plants grown at 10% and 20% relative light intensity showed lower chlorophyll a, chlorophyll b, and carotenoid contents, as well as decreased photosynthetic and dark respiration rates. In conclusion, O. insularis seedlings exhibited morphological adaptations in response to light intensity; however, no physiological responses indicating enhanced photosynthetic efficiency in shade were evident. The most favorable light condition for vigorous photosynthesis and maximum biomass production in O. insularis seedlings appeared to be 55% relative light intensity. Therefore, shading to approximately 55% of full light is suggested for the growth of O. insularis seedlings.

• Korean Journal of Environment and Ecology
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• v.21 no.6
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• pp.559-564
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• 2007

This research is aimed at finding out the ecological change of Seunghwanglim through the examination of vegetational change of tree seedlings of old giant trees growing at Seunghwanglim [Seunghwang Forest designated as a Natural Monument No.93] after the installation of protective iron fence. The survey results are as follows: First, as a result of keeping out people by installing a protective iron fence in 1989, soil hardness of the plane forest was greatly improved except the area used as roads. The seedlings of the plane forest, whose damage index is 2,3,4, were found to have the highest 1,202 $individuals/400m^2$, and those of the plane forest, whose damage index is 1, were revealed to have 565 $individuals/400m^2$, and those of the slope forest were found to have 403 $individuals/400m^2$. The number of the young sapling[taller than 2 meters] individuals of the plane forest whose damage index is 1 was found to be the most $48/400m^2$, and that of the plane forest whose damage index is 2, 3, 4- was $31/400m^2$, and that of the slope forest proved to be $14/400m^2$. In the plane forest whose damage index is 2, 3, 4, the saplings of the Ulmus davidiana var. japonica and Acer triflorum are relatively much distributed, and in the plane forest whose damage index is 1, many saplings of the Prunus padus and Styrax obassia, were found to grow and in the slope forest, many saplings of the Acer pictum subsp. mono and Acer pseudo-sieboldianum. were found to grow. Many seedlings of Rubus spp. - R. oldhamii, R. coreanus and R. crataegifolius. - and Akebia quinata were growing vigorously on plane forest, but they might decrease in number with the increasing number of the tree saplings.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.1
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• pp.34-39
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• 1990

The experiment was conducted in the pots of flooded saline paddy soil to evaluate influence of rice straw and gypsum application on germination and early growth status of directly sowed rice seedlings. 1. Germination percentage of rice seedlings were higher in treatment sowing at 1 day after submergence than that of treatment sowing at 21 days after submergence, and was severely interrupted by rice straw application. 2. Application of rice straw promoted the release of bicarbonate and volatile fatty acid of submerged water, while the amount of sulfate and soluble cations were decreased as compared to control. 3. Germination percentage of rice seedling had significant negative correlations with chemical characters of submerged water, and was highly affected by submerged water at 7 days after sowing. 4. Rice straw application interrupted germination of rice seedling by increasing pH of submerged water over critical level, and gypsum application depressed the early growth of young seedling dues to high salt concentration. 5. The relaease of bicarbonate was remarkedly increased with increasing pH over 7.5 of submerged water.

• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.335-340
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• 2013

This research was conducted to know the appropriate leaf number of scion for tube grafting in context with survival rate and quality of seedlings, and early yield of $1^{st}$ and $2^{nd}$ cluster of cherry tomato plants. Scions were grafted when having leaves 1~2 (LF-2), 2~3 (LF-3), or 3~4 (LF-4). The treatment LF-3 showed the highest survival rates and the best results in 6 factors among 12 seedling quality factors. In case of early yield, the treatment LF-2 showed higher yield than others with the big difference. Economic analysis showed that LF-3 is desirable in case of nursery while LF-2 is better in case of farmers. The compromising solution is that farmers buy a little expensive seedlings grafted at the leaf stage of 1~2 from nurseries in terms of win/win strategy.