• Title/Summary/Keyword: yoghurt

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Lactase activity in yoghurt and lactic acid bacteria (요구르트와 유산균에서의 Lactase Activity)

  • Lee, Kwang-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.1
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    • pp.60-63
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    • 1992
  • Individual starter culture were inoculated into liquid medium and incubated at $40^{\circ}C$ for 16 hours. Whole cell were obtained and evaluated for ${\beta}-galactosidase$ activity using orthonitrophenyl-${\beta}-D-galactopyranoside$ (ONPG) as substrate. S. thermophilus had more ${\beta}-galactosidase$ activity than other Lactobacilli did. To study the effect of storage temprature on enzyme activity of yoghurt, some samples of cultured yoghurt were stored under refrigeration $(4^{\circ}C)$, and the others under room temperature $(23^{\circ}C)$. At $4^{\circ}C$, yoghurt had ${\beta}-galactosidase$ activity and many viable bacteria in 1 month. After 20 days, yoghurt had maximum ${\beta}-galactosidase$ activity. At $23^{\circ}C$, yoghurt had ${\beta}-galactosidase$ activity by 5 days. As this experiment shown ${\beta}-galactosidase$ activity was ascribed to viable bacteria, especially S. thermophillus. Commercial yoghurt had lower ${\beta}-galactosidase$ activity. There were considerable variations with regard to the lactose hydrolyzing capabilities of commercial yoghurt samples.

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Effect of Heating Temperature on Viable Yoghurt Culture and $eta$-Galactosidase in Yoghurt (Yoghurt의 가열처리가 젖산균 생균수 및 $eta$-galactosidase의 활성에 미치는 영향)

  • 이광희
    • The Korean Journal of Food And Nutrition
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    • v.6 no.3
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    • pp.208-210
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    • 1993
  • In order to know the influences of heat treatment of yoghurt on pH, $\beta$-galactosidase and viable cells, yoghurt sample was made by general method with Lactobacillus bulgaricus and Streptococcus thermophilus, and the changes in pH, $\beta$-galactosidase-activity and viable cell-count were determined during heating at 55$^{\circ}C$ and 7$0^{\circ}C$. The pH of yoghurt was not changed when the yoghurt was heated at 7$0^{\circ}C$, but at 55$^{\circ}C$ it decreased slightly. The stability of $\beta$-galactosidase was not affected markedly by heat treatment at 55$^{\circ}C$, but was rapidly inactivated at 7$0^{\circ}C$. The heat treatment of yoghurt at 55$^{\circ}C$ had the halb of viable cell in 1 hour, but the heat treatment at 7$0^{\circ}C$ had considerable effect on viable cell in 5 minutes.

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Changes of Rheological Properties of Yoghurt by Different Kinds Stabilizers (Yoghurt의 안정제 종류에 따른 물성 변화)

  • 최순호;장운기;정종국;오동규;이부웅
    • Food Science of Animal Resources
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    • v.18 no.1
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    • pp.35-41
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    • 1998
  • The objective of this study was to examine the changes of physical properties by additions of different kinds of stabilizers milk proteins concentration, when stored at 4$^{\circ}C$ or 20$^{\circ}C$ for yoghurt. the results were summarized as follows: 1. Addition of 2% carboxyl methyl cellulose and carrageenan, gelation 0.4%, pectin and starch 0.6%, and carrageenan & pectin 0.8% in the manufacture of yoghurt increased the viscosity, water-holding capacity and protein hydration of yoghurt. 2. Addition of 3% skim milk powder, Ca-caseinate or Na-caseinate 0.6% increased the viscosity, water-holding capacity and protein hydration of yoghurt. 3. Twenty five percent of evaporation of milk promoted to build up the optimal structure of the micelles of yoghurt and improved viscosity, water-holding capacity and protein hydration of yoghurt. 4. Addition of stabilizers to yoghurt showed an increase of viscosity, water-holding capacity and protein hydration when compared with non-addition of stabilizers to yoghurt at 4$^{\circ}C$, 20$^{\circ}C$ storage for 12hrs, 96hrs followed by the decrease of it.

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Studies on the Characteristics of Liquid Yoghurt from Milk Added with Ginseng (인삼을 첨가 제조한 액상요구르트의 특성에 관한 연구)

  • Kim, Jong Woo
    • Korean Journal of Agricultural Science
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    • v.23 no.2
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    • pp.219-226
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    • 1996
  • Liquid yoghurts were made with ginseng extract, white ginseng powder and tail ginseng powder, which have pharmacological effects and are palatable by Korean. The acidity, number of lactic acid bacteria and viscosity of yoghurts were examined and taste, flavor and characteristics of preservation of yoghurts were also tested. The results obtained were summarized as follows; l. The acidity of yoghurt was high for ginseng yoghurts, especially tail ginseng yoghurt(1.15%) was higher than that of control yoghurt(0.76%). 2. The number of lactic acid bacteria in yoghurt was higher for ginseng yoghurt than that of control yoghurt($1.5{\times}10^8/m{\ell}$), especially tail ginseng yoghurt($4.3{\times}10^8/m{\ell}$) showed the highest number of lactic acid bacteria. 3. The viscosity of yoghurt was high for ginseng yoghurt, especially white ginseng powder yoghurt was higher than that of control. 4. The score of yoghurts for tastes, flavor and overall acceptability were higher for tail ginseng yoghurt than those of control yoghurt. 5. When yoghurts were stored at $5^{\circ}C$ for 10 days, the pH, acidity, number of lactic acid bacteria of all yoghurt were not changed significantly.

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Utilization of Ginseng Products in the Manufacture of Curd Yoghurt (호상 요구르트 제조시의 인삼제제의 이용)

  • Kim, Jong Woo
    • Korean Journal of Agricultural Science
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    • v.26 no.1
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    • pp.31-38
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    • 1999
  • Curd yoghurts were made with ginseng products, ginseng extract, white ginseng powder and tail ginseng powder, which have pharmacological effects and are palatable by Korean. The acidity, number of lactic acid bacteria, and viscosity of yoghurts were examined and taste, flavor and preservation of yoghurts were also tested. The results obtained were summarized as follows. 1. The acidity of yoghurts was higher for ginseng yoghurts, especially for tail ginseng yoghurt(1.13%) than for control Yoghurt(0.95%). 2. The number of lactic acid bacteria was higher for ginseng yoghurts, especially for tail ginseng yoghurt($9.8{\times}10^8/ml$) than for control yoghurt($6.5{\times}10^8/ml$). 3. The viscosity of yoghurt was higher for ginseng yoghurt, especially for white ginseng powder yoghurt than for control Yoghurt. 4. The score of taste and flavor of yoghurt were higher for ginseng yoghurt, whereas those of texture of yoghurt were lower than control yoghurt. However, the score of total sensory test was higher for tail ginseng yogurt than other yoghurts. 5. When yoghurts were stored at $5^{\circ}C$ for 10 days, the pH, acidity and number of lactic acid bacteria in yoghurts were not changed significantly.

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Preparation of Stirred Yoghurt from Milk Added with Korean Loquat (Eriobotrya japonica Lindley) (국내산 비파(Eriobotrya japonica Lindley)를 첨가한 Stirred Yoghurt의 제조)

  • Go Jin-Kyoung;Park Shin-In
    • The Korean Journal of Food And Nutrition
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    • v.18 no.3
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    • pp.200-206
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    • 2005
  • This study was performed to develop a functional stirred yoghurt prepared from skim milk added with loquat(Eriobotrya japonica Lindley). Skim milk containing $15\%$ loquat extract was fermented by the mixed strains of Streptococcus thermophilus and Lactobacillus acidophilus, and then loquat flesh and oligosaccharide were added to the yoghurt base. Sensory scores of the stirred yoghurt containing $15\%$ loquat flesh and $20\%$ oligosaccharide were higher than other groups in sweet taste, sour taste and overall acceptability. When the levels of oligosaccharide of the stirred yoghurts added with $15\%$ loquat flesh were increased, the L value(brightness) and a value (redness) of the stirred yoghurt decreased, but the b value(yellowness) increased. The hardness, springiness, cohesiveness, gumminess and resilience of the $15\%$ loquat flesh stirred yoghurt added with $20\%$ oligosaccharide were higher than others.

Effects of Safflower Seed Yoghurt on the Osteoporosis in Ovariectomized Rat (홍화씨 Youghurt 급여가 난소절제 Rat의 골다공증에 미치는 영향)

  • Kim, K.Y.;Kim, S.B.;Lim, J.W.
    • Journal of Animal Science and Technology
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    • v.46 no.1
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    • pp.69-76
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    • 2004
  • This study was performed to investigate the effect of satllower(Carthamus tinctorius L.) seed yoghurt on osteoporosis. Sham-operated rats(control) were fed basal diet for 6 weeks. Ovariectomized rats were divided into 6 different experimental groups including basal diet group(G I), yoghurt diet group(G II) and 1% to 4% safflower seed yoghurt diet group(GIII to GVI). and fed with the experimental diet for 6 weeks, respectively. The feed intake, feed efficiency ratio and body weight gains of rats fed with the experimental diet for 6 weeks were analyzed, respectively, and the weight. length, strength, mineral content and density in left femur of the animals were determined, respectively. Also, the right femur tissues were observed by Scanning Electron Microscope(SEM). The level of feed intake was not different among all experimental groups. The feed efficiency ratio and body weight gain were high in safflower seed yoghurt groups, and GV and GVI appeared high specially. Density, strength, ash content and minerals content in left femur were similar between control and G I, and increased with increasing the amount of satllower seed in yoghurt. On the other hand, weight and length in left femur showed no difference among all experimental groups. From the results of SEM observation of right femur tissue, GVI showed high healing effects. From the results of this experiment the healing effects of satllower seed yoghurt diet group were higher than those of control and G I. Among the treatments, GVI showed the highest healing effects on the osteoporosis in ovariectomized rats.

Studies on the Manufacture of Adlay Yoghurt II. The Volatile Flavour Compounds and the Sensory Properties of Adlay Yoghurt (율무 Yoghurt 제조에 관한 연구 II. 율무 Yoghurt의 휘발성 향미성분 및 관능적 특성)

  • 김상범;임종우
    • Food Science of Animal Resources
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    • v.20 no.1
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    • pp.64-71
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    • 2000
  • The volatile flavour compounds such as acetaldehyde, acetone, ethanol, diacetyl and acetoin were detected by gas chromatography in adlay yoghurt during fermentation and storage period at 4 $pm$1$^{circ}C$, but acetoin was trace. The contents of acetaldehyde of adlay yoghurt showed maximum immediately after fermentation and decreased significantly(P<0.05) with storage period and were in order of adlay yoghurt fermented with YC-380, ABT-D and ABT-4. The contents of acetone were shown similar trend to acetaldehyde and those of adlay yoghurt fermented with YC-380, ABT-4 and ABT-D, decreased rapidly from 6 days, 9 days and 12 days of storage, respectively. The contents of ethanol increased during the storage period and those were significant differences(p<0.05) between 6 days and 9 days of storage period in control and treatments. The contents of diacetyl were detected in control and treatments at 9 days of storage and increased slightly during the storage period. The contents of volatile flavour compounds of T1 showed similar to that of control and slightly high compared to those of treatments, and decreased gradually with increasing the level of addition of adlay in treatments. The taste, flavour and texture of T1 immediately after fermentation and during the storage period at 4$\pm$1$^{\circ}C$ were slightly higher than those of control and treatments. The scores of sensory evaluation of treatments except T1 were lowered significantly(p<0.05) with increasing level of addition of adlay. Adlay yoghurt fermented with YC-380 and ABT-4 and ABT-D were superior to both of taste and flavour, and texture, respectively. From the results mentioned above, the addition of taste and flavour, and texture, respectively. From the results mentioned above, the addition of adlay at 1% (w/v) level in skim milk substrate were suitable for quality of adlay yoghurt such as volatile flavour compounds and sensory property.

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Fermentation Characteristics of Set-Type Yoghurt from Milk Added with Mugwort Extract (쑥 추출물이 첨가된 Set-Type Yoghurt의 발효 특성)

  • 배인휴;홍기룡;오동환;박정로;최성희
    • Food Science of Animal Resources
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    • v.20 no.1
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    • pp.21-29
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    • 2000
  • This study was carried out to investigate the fermentation characteristics and storage of set-type yoghurt added mugwort extracts(AME) such as pH, growth of lactic acid bacteria, number of viable cells, viscosity, and sensory characteristics during 24 hours fermentation and 15 days storage. Addition of mugwort extracts was grown rapidly of lactic acid bacteria rather than that of control and also 4 or 8% AME groups were grown similar to control. The drop of AME pH of broth was less compared with control during incubation of lactic acid bacteria. The growth of lactic acid bacteria during incubation of AME yoghurt was not different of viable cell count between AME group and control in beginning time, but the viable cell count of AME groups were increased depended opon addition quantity of AME in ending time. Addition of mugwort extracts was not affect on pH change during yoghurt fermentation and increased a lactic acid bacteria number as well as no effect of yoghurt fermentation in ending time. The viscosity of yoghurt was almost not changed 3 hours after yoghurt mix and increased rapidly 6 hours after yoghurt mix. Although control and 0.5% AME group showed maximum viscosity at 18 hours of fermentation, 1 and 2% AME group showed linear increase until 24 hours of fermentation. Mugwort did not affect pH and viable cel number of lactic acid bacteria during 15 days storage 24 hours after fermentation. Sensory evaluation of the AME yoghurt showed that flavour, texture and acid taste were not affected by addition of mugwort. However, the appearance and taste were dropped by addition of mugwort.

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Effect of Single or Mixed Culture of Lactobacillus bulgaricus and Streptococcus thermophilus on Fermentation Characteristics of Buckwheat Sprout-added Yoghurt (Lactobacillus bulgaricus 와 Streptococcus thermophilus 의 단독 또는 혼합배양한 메밀싹 첨가 요구르트의 발효 특성)

  • Kang, Ha-Ni;Kim, Chul-Jai
    • Journal of the Korean Society of Food Culture
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    • v.25 no.1
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    • pp.76-81
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    • 2010
  • This study was conducted to evaluate the influence of Lactobacillus bulgaricus and/or Sterptococcus thermophilus on the fermentation of yoghurt containing 5% (w/v) buckwheat sprouts. The results revealed that after 12 hours of fermentation the appropriate pH, titratable acidity and number of viable cells were attained. At that time, the rutin content of the buckwheat sprout-added yoghurt prepared by the mixed culture method was not changed, but the quercetin content increased greatly. Conversely, the rutin content of yoghurt that only contained Streptococcus thermophilus was decreased while the quercetin content was increased. The total phenol contents as well as the DPPH radical scavenging activities of both the mixed culture and Streptococcus thermophilus yoghurt did not differ significantly. Taken together, the results revealed that the use of a mixed culture of Lactobacillus bulgaricus and Streptococcus thermophilus during the preparation of buchwheat sprout-added yoghurt was desirable due to the decrease in pH and increase in titratable acidity and viable cells that occurred after 12 hr of fermentation. Moreover, phytochemicals in buckwheat sprouts such as rutin, quercetin and phenol compounds were comparatively increased during fermentation and influenced the antioxidant activity in buckwheat sprout-added yoghurt.