• Title/Summary/Keyword: yield rate

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A Study on Minimizing the Residual $^{18}F$-FDG in the Tubing Using Nitrogen Gas (FDG 합성 후 질소가스를 이용한 튜빙의 잔류 $^{18}F$-FDG 최소화를 위한 방법의 유용성)

  • Kim, Dong-Il;Park, Hoon;Seok, Jae-Dong
    • The Korean Journal of Nuclear Medicine Technology
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    • v.14 no.1
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    • pp.8-12
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    • 2010
  • Purpose: In $^{18}F$-FDG automated synthesizer, deliver is done in automated mode after synthesis until the dispenser. After the delivery, the yield is calculated from the radioactivity which was read by the dose calibrator located in the dispenser. However, when the distance between the automated synthesizer and the dispenser is far, there are $^{18}F$-FDG residues, which results in loss of the amount of $^{18}F$-FDG. This study investigated the usefulness of a method that minimizes $^{18}F$-FDG residues. Materials and Methods: The structure of the tubing between the (TRACERlab Mx FDG; GE.) and the dispenser is that the distance is 8 m and the internal diameter is 1/16 inch. The synthesis process of The module goes through the synthesis process of trap, synthesis, delivery in the automated module. The time taken for synthesis is about 25 to 26 minutes, after which rinsing is done. However, after rinsing, as the distance of the tubing increased, there were 10~13% of $^{18}F$-FDG residues. Therefore, a method of using push syringe and $N_2$ gas in manual mode to minimize $^{18}F$-FDG residues is analyzed. Results: In manual mode, there were $^{18}F$-FDG residues of 4~5% for the push syringe, and there were $^{18}F$-FDG residues of less than 1% for the $N_2$ gas, which showed that the method using $N_2$ gas had superior usefulness. Also, there were no $^{18}F$-FDG residues in the cleaning the next day. Conclusion: The distance between the synthesizer and the dispenser needs to be reduced as much as possible, to reduce the rate of loss of $^{18}F$-FDG resulting from the distance of the tubing. However, in case the distance between the synthesizer and the dispenser has to be increased due to the system structure, using push syringe and $N_2$ gas simultaneously is a useful method for minimizing $^{18}F$-FDG residues.

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Identification of Mycobacterium Tuberculosis in Pleural Effusion by Polymerase Chain Reaction (PCR) (흉막삼출액에서 Polymerase Chain Reaction (PCR)을 이용한 결핵균의 검출에 관한 연구)

  • Kim, Ho-Joong;Kim, Young-Whan;Han, Sung-Koo;Shim, Young-Soo;Kim, Keun-Youl;Han, Yong-Chol
    • Tuberculosis and Respiratory Diseases
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    • v.40 no.5
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    • pp.509-518
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    • 1993
  • Background: By amplifying small amount of DNA, polymerase chain reaction (PCR) can be used for the detection of very small amount of microbial agent, and may be especially useful in certain cases which are difficult to be diagnosed microbiologically or serologically. Tuberculous pleurisy is a disease that can be diagnosed in only 70% of cases by conventional diagnostic tools, and PCR would be a very rapid, easy, and sensitive diagnostic method. Method: The specificity and sensitivity of PCR to detect Mycobacterium tuberculosis DNA were evaluated using various strains of Mycobacteria. To evaluate the diagnostic usefulness of PCR in tuberculous pleurisy, we used PCR to detect Mycobacterium tuberculosis DNA in pleural fluid. The amplification target was 123 base pair DNA, a part of IS6110 fragment, 10~16 copies of which are known to exist per genome. The diagnostic yield of PCR was compared with conventional methods, including pleural fluid adenosine deaminase (ADA) activity. Also, the significance of PCR in undiagnosed pleural effusion was evaluated prospectively with antituberculosis treatment. Results: 1) Using cultured Mycobacterium tuberculosis and other strains, PCR could detect upto 1 fg DNA and specific for only Mycobacterium tuberculosis and Mycobacterium bovis. 2) Using pleural effusions of proven tuberculosis cases, the sensitivity of PCR was 80.0% (16/20), and the specificity 95.0% (19/20). 3) Among 13 undiagnosed, but suspected tuberculous effusion, the positive rate was 60% in 10 improved cases after antituberculosis medications, and 0% in 3 cases of proven malignancy later. 4) Adenosine deaminase level of proven and clinically diagnosed tuberculous pleurisy patients was significantly higher than that of excluded patients, and correlated well with PCR results. Conclusion: We can conclude that PCR detection of Mycobacterium tuberculosis in pleural effusion has acceptable sensitivity and specificity, and could be an additional diagnostic tool for the diagnosis of tuberculous pleurisy.

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Fully Automated Liquid Culture System Compared with Lowenstein-Jensen Solid Medium for Rapid Recovery of Mycobacteria in Sputums (완전 자동화된 액체배양법과 기존의 고체배양법을 이용한 객담 내 mycobacterium의 신속검출에 대한 비교)

  • Park, Seung-Kyu;Kim, Seung-Chul;Kim, Deuk-Mi;Lee, Chang-Woon;Kim, Young;Cho, Sang-Nae
    • Tuberculosis and Respiratory Diseases
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    • v.53 no.6
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    • pp.635-643
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    • 2002
  • Background : The Aim of this study was to compare the recovery of mycobacteria from sputum samples of pulmonary tuberculosis patients using the MB/BacT rapid culture system(Organon Teknika, USA) with that obtained using Lowenstein-Jensen solid medium. Methods : The two culture systems were compared using sputum samples of 99 pulmonary tuberculosis patients. Culture media were incubated at $35-37^{\circ}C$ for six weeks in the MB/BacT system and for 12 weeks in Lowenstein-Jensen solid medium. Solid media were examined macroscopically once a week, and the MB/BacT system positive vials were unloaded from the machine as soon as possible after positive signal from the connected computer was detected Confirmation of growth for mycobacteria was done by Ziehl-Neelson stained smears. Isolates were identified to differentiate Mycobacterium tuberculosis from mycobacterium other than tuberculosis(MOTT) by phenotypic and molecular methods. Results : Of the sputum samples of the 99 patients, 58 samples were smear positive and 41 in negative smear. Mycobacteria were recovered from 67(67.7%) samples by using both culture systems. The yield with MB/BacT was higher than that with Lowenstein-Jensen [67(67.7%) vs. 52(52.5%), p<0.001]. Moreover, 15(15.2%) samples were positive only in the MB/BacT, whereas none of samples was positive only in Lowenstein-Jensen. In smear-positive and smear-negative samples, the recovery rate with MB/BacT was also higher than that with Lowenstein-Jensen [sputum-positive; 56/58(96.6%) vs. 46/58(79.3%), p=0.005, sputum-negative; 6/41(14.6%) vs. 5/41(12.2%), p<0.001]. The mean times to detection of Mycobacteria were 13.3 and 27.2 days with MB/BacT and Lowenstein-Jensen respectively(p<0.001). Conclusion : This results indicate that the the MB/BacT is more efficient and faster than Lowenstein-Jensen for the recovery of mycobacteria.

Consideration of the Quantitative Nut Characteristics in Chestnut Hybrids (밤나무 교잡종(交雜種) 과실(果實)의 양적특성(量的特性)에 관한 고찰(考察))

  • Lee, Uk;Kim, Mahn-Jo;Lee, Moon-Ho;Hwang, Myoung-Soo;Hwang, Suk-In
    • Journal of Korean Society of Forest Science
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    • v.94 no.1 s.158
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    • pp.34-38
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    • 2005
  • This study, basic research for releasing new chestnut cultivar, is conducted to consider quantitative nut characteristics according to combinations and individuals In 11 chestnut hybrids. Number of bur on bearing branch(NBB) and nut yield(NY), which showed large difference among combinations, was superior in JO ($Joook{\times}Otanba$) and KO ($Kwangeun{\times}Otanba$) combinations, respectively. Average nut weight (NW) was 21.1g, the highest in EO ($Eunsan{\times}Otanba$) combination. Nut shape which was expressed to the rate of nut height and width, was investigated to the range of 1.13-1.23 in the all combinations, so nut shape of all combinations showed oval type. EO and JO combinations, which were measured to 30.0% and 27.5%, respectively in the percentage with the pericarp split(PPS), were produced more bad fruits than the others. OK ($Otanba{\times}Kwangeun$) combination, 1.7%, was the most excellent in the percentage of polyembryonic nuts(PPN), and the superior combinations which was measured to the below 5%, could be included five combinations. ER and RK combinations, were the highest values, 16.3% and $10.0kg/cm^2$, in soluble solids content(SSC) and kernel hardness (KH), respectively. NBB showed highly positive correlation with NY, but showed highly negative correlation with SSC and NW. NW showed highly positive correlation with PPS, but showed highly negative correlation with SSC and NH. PPS showed highly positive correlation with PPN. Eight individuals such as superior individuals could be selected by selection criteria.

Optimal Configuration of the Truss Structures by Using Decomposition Method of Three-Phases (3단계(段階) 분할기법(分割技法)에 의한 평면(平面)트러스 구조물(構造物)의 형상(形狀) 최적화(最適化)에 관한 연구(硏究))

  • Lee, Gyu Won;Song, Gi Beom
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.12 no.3
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    • pp.39-55
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    • 1992
  • In this research, a Three Level Decomposition technique has been developed for configuration design optimization of truss structures. In the first level, as design variables, behavior variables are used and the strain energy has been treated as the cost function to be maximized so that the truss structure can absorb maximum energy. For design constraint of the optimal design problem, allowable stress, buckling stress, and displacement under multi-loading conditions are considered. In the second level, design problem is formulated using the cross-sectional area as the design variable and the weight of the truss structure as the cost function. As for the design constraint, the equilibrium equation with the optimal displacement obtained in the first level is used. In the third level, the nodal point coordinates of the truss structure are used as coordinating variable and the weight has been taken as the cost function. An advantage of the Three Level Decomposition technique is that the first and second level design problems are simple because they are linear programming problems. Moreover, the method is efficient because it is not necessary to carry out time consuming structural analysis and techniques for sensitivity analysis during the design optimization process. By treating the nodal point coordinates as design variables, the third level becomes unconstrained optimal design problems which is easier to solve. Moreover, by using different convergence criteria at each level of design problem, improved convergence can be obtained. The proposed technique has been tested using four different truss structures to yield almost identical optimum designs in the literature with efficient convergence rate regardless of constraint types and configuration of truss structures.

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Studies on the Salt Tolerance of Rice and Other Crops in Reclaimed Soil Areas -1. On the Salt Tolerance of Rice Varieties (간척지에서 수도 및 기타작물의 내염성에 관한 연구 -1. 수도품종의 내염성에 관하여-)

  • Hyong-Bin Im;Jai-Wook Shim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.8 no.1
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    • pp.47-68
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    • 1970
  • Experiments were conducted by moderate season culture of each of 7 early, medium and late maturing varieties which were considered to be of strong salt tolerance in non-salty, low- and high-salty reclaimed areas (salt content: 0.5% and 1% at the end of April). The results of the experiments revealed that the early maturing varieties were suffered greatly from salt damage in such reclaimed salty area which is the "fall better type" in the year-round changes of soil salt concentration in Korea. Better yielding varieties in the salty area showed generally a high yield ratio in the salty area compared with the non-salty area. This indicates that they are strong salt tolerant varieties. It seemed that the salt tolerance could be measured by the number of delaying days in heading, the growth rate of culm length, number of panicles and number of grains per panicle in the salty area to compare with the non-salty area. The effects or salt damage on the yields of each variety at maturing stage and agronomic characteristics in the salty area were compared and discussed with the non-salty area.alty area.

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Effect of Whole or Steam-flaked Corn Based Diet on Ruminal fermentation Characteristics In Vitro and Ruminal Metabolism in Korean Native Goat In Vivo (통옥수수 및 Steam-flaked 옥수수 기초사료가 반추위미생물 발효성상과 한국재래산양 반추위대사 특성에 미치는 영향)

  • Bae, G.S.;Bae, J.H.;Yun, S.J.;Chang, M.B.;Ko, J.Y.;Ha, Jong-K.
    • Journal of Animal Science and Technology
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    • v.44 no.6
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    • pp.757-768
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    • 2002
  • These study were conducted to determine the effects of a whole or steam-flaked corn based diet on rumen microbial fermentation in vitro and ruminal metabolism in the Korean Native Goat(KNG) in vivo. The experiments consisted of two dietary treatments: control, steam-flaked corn(SFC) based diet(80%) + rice straw mixed(20%)(SFCR); 100% whole corn based diet(WC). The first experiment was conducted to investigate the effect of whole corn on ruminal metabolism in vitro for 0 to 48 h. pH values were optimally maintained during incubation time, and were not significantly different between treatments. Gas production of SFCR was significantly higher than WC(p<0.01). $NH_3$-N concentration tended to increase for WC, but not significantly different between treatments. The mean value of total volatile fatty acid concentration of WC was significantly lower than SFCR(p<0.01), but SFCR and WC linearly increased as the time of incubation approached 48 h. Mean value of acetate concentration of SFCR was significantly higher than WC(p<0.01). Propionate concentration of WC for the total incubation time was significantly higher than SFCR(p<0.01). The digestibility of dry matter was not significantly different between treatments, but SFCR was somewhat higher than WC. The second experiment was conducted to effect of whole shelled corn based diet on rumen metabolism in KNG. pH values tended to decrease through all treatments. There was not a significantly difference between treatments. Microbial protein yield of SFCR was significantly higher than WC(p<0.01). $NH_3$-N concentration of WC was significantly (p<0.01) higher than SFCR. Total VFA and propionate concentration of WC was significantly higher than SFCR(p<0.01), but acetate concentrate of WC was not significantly higher than SFCR. The mean value of total lactate concentration was significantly(p<0.01) different but the value of SFCR and WC were lower than the average concentration of acidosis. In sacco DM disappearance rate of SFC was significantly(p<0.01) higher than WC.

Food Waste Composting by Using an Inoculum-Mixture Containing New Facultative Anaerobic Bacteria (신규 통성혐기성 세균으로 제조한 발효흙에 의한 음식물 쓰레기의 퇴비화)

  • Hwang, Kyo-Yeol;Lee, Jae-Yeon;Kim, Keun;Sung, Su-Il;Han, Sung-Ho
    • Journal of the Korea Organic Resources Recycling Association
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    • v.9 no.1
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    • pp.65-72
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    • 2001
  • Four newly isolated bacteria from soil were used to manufacture microbial inoculum to compost food waste. The bacteria, GM103, V25, V31, and V35, were identified as Bacillus licheniformis, B. subtilis, B. stearothermophilius, and B, subtilis, respectively. The bacterial strains were efficient to degrade protein and starch and also able to inhibit the growth of plant pathogenic fungus Rhizopus stronifer. The GM103 showed distinct capability in degrading starch, but grow only aerobically. The other three bacterial strains. V25, V31, and V35, could grow both aerobically as well as anaerobically, in 10%(w/v) salt, at $50^{\circ}C$, and had good viability and survival rate in soil. These characteristics of the bacterial strains are very adquate in Korean food composting containing high concentration of salt, especially at home. By mixing the 4 bacterial culture broth with molasses, beet pulp, zeolite, The bacterial inoculum for food waste composting-BIOTOP-CLEAN-was made. The performance of food waste composting by the BIOTOP-CLEAN was compared with that by control(not treated) and HS(other demestic company's inoculum product for food waste composting). The maximum temperature of the food waste during the composting with the BIOTOP-CLEAN was $50^{\circ}C$, while those of the control and HS were $30^{\circ}C$ and $35^{\circ}C$, respectively. The BIOTOP-CLEAN gave the good smell and showed dark brown color, while the control gave bad smell and HS gave less bad smell. These indicates that the food waste composting by the BIOTOP-CLEAN had been well accomplished. The culture broth of V25, V31, V35 were sparyed to the plants of tomato, chinese cabbage, raddish, red pepper every month and the spraying the culture broth to these plant significantly improved the production yield of the crops, due to the control effect of the bacterial strains against the plant pathogens.

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Anaerobic Biodegradability of Leachates Generated at Landfill Age (매립년한에 따른 침출수의 혐기성 생분해 특성)

  • Shin, Hang-Sik;Lee, Chae-young;Kang, Ki-hoon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.8 no.1
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    • pp.90-96
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    • 2000
  • The composition of leachates varies depending on the waste characteristics, landfill age and landfilling method. Generally, leachates contain high dissolved organic substance and ammonia nitrogen whereas phosphorus concentration was very low. Leachate A produced from young landfill is characterized by high BOD5/COD ratio (0.8) whereas leachate C produced from old landfill has lower BOD5/COD ratio (0.1). Maximum biochemical methane potential of leachate A, B (from medium landfill) and C were 271,106 and 4 ml CH4/g-COD, respectively. On the other hand, the maximum biodegradability of leachate A, B, and C were 75,30, and 1%, respectively. These results indicated that anaerobic treatment of leachate from young landfill was effective in removing organic pollutants. In case of leachate C, carbon might reside in the form of large molecular weight organic compounds such as lignins, humic acids and other polymerized compounds of soils, which are resistant to biodegradation. The lag-phase period increased with the increasing organic concentration in leachate. In case of leachate A of concentration greater than 25%, the lag-phase period increased sharply. This implied that the start-up period of anaerobic process using an unacclimated inoculum could be extended due to the higher concentration of leachate. This relatively long lag-phase is probably related to the fact that most of the inhibitory compounds have been diluted beyond their inhibitory concentrations of less than 50%. Furthermore, the ultimate methane yield and methane production rate decreased as leachate concentration increased. It was anticipated the potential inhibition was related with the steady-state inhibition as well as the initial shock load.

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Effects of Far-infrared Irradiance at Night on Quality of Sunlight Dried Red Pepper (Capsicum annuum L.) in Plastic Houses (비닐하우스 이용 고추 건조 시 야간 원적외선등 조사량이 품질에 미치는 영향)

  • Lee, Guang-Jae;Kim, Si-Dong;Yoon, Jung-Beom;Lee, Ki-Yeol;Choi, Kyu-Hong
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.819-826
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    • 2014
  • This study was carried out to investigate the effects of night-time far-infrared irradiance quality of red pepper dried in greenhouses. This study involved 4 treatments: sunlight alone (control), or sunlight plus nightly far-infrared irradiation at $250W{\cdot}6.6m^{-2}$ ($250W{\cdot}6.6m^{-2}$), far-infrared irradiation at $250W{\cdot}3.3m^{-2}$ ($250W{\cdot}3.3m^{-2}$), or far-infrared irradiation $500W{\cdot}3.3m^{-2}$ ($500W{\cdot}3.3m^{-2}$). The drying periods were 12 days in $500W{\cdot}3.3m^{-2}$ and $250W{\cdot}3.3m^{-2}$, and 14 days in $250W{\cdot}6.6m^{-2}$, and 15 days in the control. The daytime temperature was same among the treatments. The lowest temperature was at $23.8^{\circ}C$ in control, and $29.5-37.2^{\circ}C$ in far-infrared irradiation treatments. The marketable yield was 7-14% higher in far-infrared irradiation treatments compared to the control. The rate of marketability was higher in far-infrared irradiation treatments (93.6-96.3%) than in the control (87.0-87.5%). The American Spice Trade Association (ASTA) value was greatest in the $250W{\cdot}3.3m^{-2}$ treatment, followed by $250W{\cdot}6.6m^{-2}$, then $500W{\cdot}3.3m^{-2}$, and finally the control. Capsaicinoid content showed no regular trend among the treatments. Our results provide an optimized method for reducing drying time of red pepper under sunlight, and improving the quality of dried red pepper.