• Journal of the Korean Wood Science and Technology
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• v.26 no.4
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• pp.61-70
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• 1998

Xylem, bark and flower buds of Magnolia kobus DC. var. borealis Sarg. were extracted with ethanol. And then the ethanol extracts were fractionated with diethyl ether. The lignans structure of the diethyl ether fractions were determined by spectroscopic methods. The results are summarized as follows : 1. Three compounds, aschantinn(X-I), yangambin(X-II), (-)syringaresinol(X-III), tetrahydrofurofuranoid lignans were isolated from the xylem. 2. Five compounds were isolated from the bark. These are all tetrahydrofurofuranoid lignans, aschantin(B-I), yangambin(B-II), medioresinol(B-III), (+)syringaresinol(B-IV), and tetrahydrofuranoid, kobusinol B(B-V). 3. Three tetrahydrofurofuranoid lignans, kobusin(F-I), aschantin(F-II), yangambin(F-III) were isolated from the flower buds. 4. Isolated lignans from the each tissue were two types of tetrahydrofurofuran type and tetrahydrofuran type. 5. The tetrahydrofurofuranoid lignans were five compounds such as kobusin, aschantin, yangambin, medioresinol, and syringaresinol, while the tetrahydrofuranoid lignans was kobusinol B. 6. Most of these lignans were substituted with the methylenedioxy or methoxyl groups.

• Journal of Plant Biology
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• v.29 no.3
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• pp.185-196
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• 1986

Anatomical characteristics of the secondary xylem in the roots and the stems of genus Pinus including nine species growing naturally in Korea have been studied to elucidate the structural differences between two organs. The results, comparing the root woods with the stem woods, are as follows; The transition from the early-wood to the late-wood occurs more gradually in the root woods. In the root woods, the bordered pits on the radial wall of tracheid are frequent in the two rows. The spiral thickening on the radial and tangential wall of the tracheid can be seen in the only stem woods of four species such as P. koraiensis, P. rigida, P. rigitaeda and P. banksiana. In the majority of the species studied, the length of the tracheid is longer in the root woods, but in some species such as P. strobus, P. densiflora, P. rigida and P. sylvestris, this length is almost sam ein both organs. The diameter of the tracheid in the root woods is wider. The wall thickness of the tracheid in the early wood is thicker in the root woods, but vice versa in the late woods. More rays per unit area can be seen in the root woods.

• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2071-2078
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• 2018

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight, which results in severe economic damage to rice farms. Xoo produces biofilms for pathogenesis and survival both inside and outside the host. Biofilms, which are important virulence factors, play a key role in causing the symptoms of Xoo infection. In the present study, we investigated the nutritional conditions for biofilm formation by Xoo. Although Xoo biofilm formation may be initiated by interactions with the host, Xoo biofilm cannot mature without the support of favorable nutritional conditions. Nitrogen sources inhibited Xoo biofilm formation by overwhelming the positive effect that cell growth has on it. However, limited nutrients with low amino acid concentration supported biofilm formation by Xoo in the xylem sap rather than in the phloem sap of rice.

• Korean Journal of Pharmacognosy
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• v.1 no.3
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• pp.81-92
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• 1970

So far, the cultivated peony is known to be originated from an indigenous species, Paeonia albiflora $P_{ALLAS}$ var. trichocarpa $B_{UNGE}$ (PAT). In this study, these two species were morphologically examined in the external and internal feature and in the pattern of callus formation by tissue culture. Also, they were compared with another indigenous species, P. japonica $M_{IYABE}$ et $T_{AKEDA}$ var. pilosa $N_{AKAI}$ (PJ), which were regarded as being scarcely related to them. The root of the cultivated peony is massive consisting with several storage roots, each of them is a hypotrophic and fusiform. The root of PAT consists of several storage roots, each of them is branching and slender. And the storage root of PJ is short, bended buried horizontally, protruding a number of corpulent lateral root. The secondary xylem of the cultivated peony is small clusters of vessels and xylem fibres are arranged in scalariform and among these cluster, single vessel is joined, but that of PAT is small clusters of vessels are arranged in separate scalariform but are not connected with each other and that of PJ is vessels and xylem fibers are grouped together in elongated clusters that radiate outward from the center. Protoxylem of the cultivated peony is surrounded by four large metaxylem, but that of PAT and PJ by seven. On the other hand, the callus formation patterns of these peonies were different; the cultivated peony callus is formed in an orderly fashion by the mammalate meristematic cell groups, PAT callus is in disorder by the meristematic cells arranged in linear, and PJ callus is in order by the meristematic cells arranged in linear. By the comparison of three different plants in the anatomical appearance and the callus formation pattern, it is evident that the cultivated peony is not derived from PAT.

• The Plant Pathology Journal
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• v.20 no.2
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• pp.81-84
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• 2004

Branched broomrape (Orobanche ramosa) is a holo-parasitic flowering plant that attaches to the root of its host, green plant, by means of a specialized structure known as haustorium. Following successful contact and penetration on susceptible plant root, complex tissue of Orobanche cells is formed which is known as the tubercle. Newly formed tubercles contain high activity ofindole-3-acetic acid (IAA). Triiodobenzoic acid (TIBA), as an inhibitor of IAA polar transport, was utilized to investigate the supply and requirement of auxin to the developing O. ramosa on tomato plant. There was no significant reduction in the incidence of O. ramosa per pot of different TIBA treatments. However, infection severity in terms of the number of O. ramosa shoots that emerged per plant and number of attachments per plant root system were significantly reduced by 60 % and 45 % on TIBA treated plants, respectively. Histo-logical studies revealed conspicuous delay in the initiation of xylem vessel differentiation inside tubercles of the TIBA treated tomato plants. Also, differentiated vessels showed thinner secondary wall deposition, and improper alignment within bundles inside those tubercles. They were wider and shorter in diameter in comparison to those of untreated plants. These findings were attri-buted to the short supply of IAA required for normal development, and to the xylem vessel differentiation of O. ramosa tubercles on infected tomato. Hence, this parasitic flowering plant seems to depend upon its host in its requirements for IAA, in a source to sink relation-ship.

• Journal of Plant Biology
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• v.3 no.2
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• pp.29-34
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• 1960

From the view point of phytopathological anatomy, the author has tried to study the effect of the shoot cluster disease virus on the internal structure of vascular tissues of chinese date tree (Ziziphus jujuba var. inermis Rehd.) comparing healthy checks and diseased plants. The materials were collected at the several sites, Kumgock-Ri, Masuc-Ri, Kyungi-Do, and near the campus of Korea University and around the area of Chongam-Dong, Seoul City, from August 15th to September 5th 1959. The leaf materials of healthy and diseased plants are fixed and aspirated in two kinds of killing solutions, formalin-acetic acid alcohol solution and Craf III solution. Sections were cut at 5-10$\mu$ thickness and stained with the double staining reagents of safranin and fast green. In this experiment the author has observed that there are marked structural changes in the infected plants in contrast of healthy checks. As figures 3-7 show that the following characteric changes have taken place on infected plants: 1) the arrangement of irregularly developed sieve elements in phloem, 2) the degeneration of phloem elements, 3) the irregular arrangement of epidermis in mid-vein, 4) more necrosis is observed among the parenchymatous cells, 5) abundant accumulatin of starch grains in parenchymatous cells, . In contrast to the above irregularities caused by the virus disease, the healthy checks appear normal structures as shown in figures 1 and 2. In adding to the all features noted above, the author could also observe an interesting feature that the xylem elements in mid-vein vascular bundle tissues are considerably disorganized to show the unspecialized vessel elements, the irregularly arranged xylem elements. However, this kind of irregularities which occur in xylem under the virus infection has not been reported previously. The features noted on the internal structure of vascular bundle under the condition of infection by the shoot cluster disease on chinese date trees appear to be more or less closely similar to the symptoms of the bunchy-top of banana and the yellow dwarf disease of barley in respect to the fact that whether phloem necrosis takes place as a primary symptom or a secondary symptom. In all these disease, primary histological changes of hypoplasia and hypertrophy are preceeded by the necrosis of phloem.

• Journal of the Korean Wood Science and Technology
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• v.42 no.3
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• pp.223-230
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• 2014

This study examined emission characteristics of far-infrared ray, formaldehyde, volatile organic compounds and deodorization rate of particleboard prepared at different target board densities and resin content levels, using the xylem part of Broussonetia Kazinoki Sieb. The deodorization rate increased, as the target density of board and resin content increased. Emission rates of the far-infrared ray and total volatile organic compounds (TVOCs) were 0.892~0.899, and $0.074mg/m^2{\cdot}h$ for particleboard prepared with the resin content of 13% and target density of $0.6g/cm^3$ while the deodorization rate was 80~90% for the same particleboard. The formaldehyde emission factor ranged from $0.004mg/m^2{\cdot}h$ to $0.006mg/m^2{\cdot}h$ for all particleboard fabricated at different resin content and target densities. These results indicate that particleboard prepared from the xylem part of Broussonetia Kazinoki Sieb have a potential as a building construction material.

• Journal of the Korean Wood Science and Technology
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• v.34 no.3
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• pp.1-7
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• 2006

Anatomical and physical properties of kenaf grown in Chuncheon, Korea were investigated using light and scanning electron microscopy and X-ray diffraction method. Bast fiber, phloem ray, cortex parenchyma cell and sieve tube member were observed in phloem, and vessel element, fiber and ray in xylem. Solitary and multiple radial pores in xylem existed. The cell types of ray parenchyma in radial section were procumbent, upright and square cells. The length of bast fiber increased with increasing the growth period. The length of wood fiber was 0.74~0.82 mm, but was not significantly different between the growth period and stem height. Relative crystallinity was 53~74% in phloem and 43~58% in xylem. Cellulose crystallinity width was 2.68~3.24 nm in phloem and 2.46~2.95 nm in xylem. The green moisture content and green density increased but basic density decreased with increasing the stem height.

• Korean journal of applied entomology
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• v.11 no.2
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• pp.55-60
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• 1972

The study has been carried to investigate the nature of resistance in rice varieties against bacterial leaf blight caused by Xanthomonas oryzae (Uyeda et. Ishiyama) Dowson. In this study, differences on several morphological histological and bacterial growth in rice leaf extract from different varieties were examined. Shirogane and Norm 6 as resistant varieties, Paldal as moderately resistant, and Jinheung, Kimmaze and Suwon 213 as susceptible varieties were used throughout the experiment. 1. Susceptible varieties Jinheung, Kimmaze have more hydathods in flag leaf than resistant varieties but there were no difference between resistant varieties and moderatly resistant varieties. 2. Average length of xylem vessel elements were 50r longer in leaf veins of susceptible varieties Jinheung, Kimnaze and Suwon 213 than those of resistant varieties, but there were no difference among Paldal, Norm 6 and Shirogane. 3. Kimmaze and Suwon 213 have larger diameter of xylem vessel elements than those of other varieties examined. However, it did not differ significantly between resistant varieries and susceptible varieties statistically. 4. Jinheung and Kimmaze have more secondary xylem vessels in root of 6-7th leaf stage than those of Shirogane and Norm 6. Suwon 213, however, showed least number of secondary xylem vessels exceptionally. 5. Leaf extract from resistnat variety Shirogane, suppressed bacterial growth significantly when compared with those from other varieties. 6. Bacterial growth in autoclaved leaf extract from resistant varieties and susceptible varieties did not show any noticeable difference. In general, fresh extract maintained more bacteria than in autoclaved extract after 48 hours of incubation period.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.6
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• pp.910-914
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• 2005

This study was to determine the inhibitory effect of ethanol extract of leaf, bark and xylem of Quercus aliena Blume and, consecutively, of organic solvent subfractions against food borne pathogens. The ethanol extracts of leaf, bark and xylem of Quercus aliena Blume were shown to have antimicrobial effect against Gram-positive and Gram-negative bacteria. Listeria monocytogenes and Salmonella Typhimurium were more resistant to ethanol extracts of the Quercus aliena Blume tissues compared with Bacillus cereus and Escherichia coli O157:H7. The ethanol extract of Quercus aliena Blume leaf showed the strongest antimicrobial activity against food borne pathogens, followed by those of xylem and bark. The ethanol extract treatment $(500\~1,000\mu g/mL)$ of Quercus aliena Blume leaf completely reduced the growth of B. cereus and L. monocytogenes within 24 hour whereas $2,000\mu g/mL$ of ethanol extract was needed for complete growth inhibition of E. coli O157:H7 and S. Typhimurium. Among organic solvent subfractions obtained from the ethanol extract of leaf, bark and xylem of Quercus aliena Blume, the ethyl acetate fraction showed the strongest antimicrobial activity, but no antimicrobial activity was observed in chloroform, hexane and water fractions.