• Journal of Periodontal and Implant Science
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• 제26권4호
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• pp.873-887
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• 1996

Platelet-derived growth factor(PDGF) is one of the polypeptide growth fators. PDGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. Recent studies indicated that demineralized root surface as the primary site for growth factor application has advantages over other application method, especially due to binding capacity of growth factor for exposed matrix component of deminera1ized dentin surface. The purpose of this study is to evaluate optimal application time of PDGF-BB on proliferation of human gingival fibroblasts using deminera1ized dentin surface as primary application site. Human gingival fibroblasts and dentin slabs were prepared from the first premolar tooth extracted for the orthodontic treatment, cells were cultured in DMEM/I0% FBS at the $37^{\circ}C$, 5% CO2 incubator. All of the dentin slabs were preconditioned with Tetracycline HCI(100mg/ml) solution and rinsed in PBS. In the cell proliferation experiment, experimental group was immersed in DMEM containing 10% FBS, 50ng/rnl PDGF-BB during different time(30sec, 1, 2, 4, 8 minutes) and dried. Cells at concentration of $1{\times}10^5$cells/ml were seeded in each culture well which contained dentin slabs and incubated for 6 hours. Then, all of the dentin slabs were moved into new 24 well culture dish and incubated for 24, 48, 72 hours. The cell counting was done by hemocytometer with inverted phase contrast microscope after trypsinization. The results were as follows : The application of PDGF-BB for 1, 2 min slightly increased the number of gingival fibroblasts, and the application of PDGF-BB for 4, 8 min prominently increased the number of gingival fibroblasts. The application of PDGF-BB for 4 min showed maximum proliferation rate of gingival fibroblasts at 24, 48, 72 hours, and the application of PDGF-BB for 8 min showed less proliferation rate of gingival fibroblasts compared to the application of PDGF-BB for 4 min at 24, 48, 72 hours. In conclusion, the application of PDGF-BB for 4 min appeared to be optimal to obtain maximum proliferation of gingival fibroblasts using demineralized dentin surface as primary applicaton site of PDGF-BB.

• Journal of Periodontal and Implant Science
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• 제26권4호
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• pp.841-858
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• 1996

Epidermal growth factor(EGF) is one of polypeptide growth factors. EGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. The purposes of this study is to evaluate the effects of EGF on the human periodontal ligament cells and human gingival fibroblast cells that promote regeneration of periodntal tissue. The mitogenic effects of epidermal growth factor on human periodontal ligament cells and human gingival fibroblasts were evaluated by determining the incorporation of 5-Bromo-2'-deoxy-uridine into DNA of the cells in a dose dependent manner. The prepared cells were the primary cultured gingival fibroblast and periodontal ligament cells from humans, the fourth or sixth subpassages were used in the experiments. Cells were seeded in DMEM containing 10% FBS. 1, 10, 50, 100, $200{\eta}g/ml$ and epidermal growth factor were added to the quiescent cells for 24 hours, 48 hours and 72 hours. They were labeled with $10\{mu}l/200{\mu}l$ 5-Bromo-2'-deoxy-uridine for the last 6 hours of each culture. The results of the five determinants were presented as mean and S.D.. The results were as follows : The DNA synthetic activity of human gingival fibroblasts were increased dose dependently by epidermal growth factor at 24 hours, 48 hours and 72 hours. The mitogenic effects were similar at the 24 and 48 hours of epidermal growth factor, but the DNA synthetic activity of human gingival fibroblasts generally decreased at 72 hours. The DNA synthetic activity of human periodontal ligament cells were increased dose dependently by epidermal growth factor at 24 hours but the DNA synthetic activity decreased at $200{\eta}g/ml$ of each hour. Generally the maximum mitogenic effects were observed at the 48 hours application of epidermal growth factor. The DNA synthetic activity of human periodontal ligament cells generally decreased lower at 24, 72 hours than at 48 hours the application of epidermal growth factor. In the comparison of DNA synthetic activity between human gingival fibroblasts and human periodontal ligament cells, human periodontal ligament cells had slightly higher proliferation activity than human gingival fibroblasts for a longer time at the high dosage of the epidermal growth factor. In conclusion, epidermal growth factor have important roles in the stimulation of DNA synthesis in human periodontal ligament cells and human gingival fibroblasts, and thus may be useful for clinical applications in periodontal regenerative procedures.

• 한방안이비인후피부과학회지
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• 제16권3호
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• pp.38-67
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• 2003

The burn is acute skin injury caused by fire, hot water. steam. hot oil, sour and salty. It is occurred frequently in the daily life as well as oriental therapy like moxibustion therapy, physical therapy. Nevertheless, medical treatment of the burn is almost dependent on western cure. So we chose the oriental medicine textbooks and the oriental medicine journals that were dealing with the drugs, processing the drugs. peculiar treatment put first external cure. The results were as follows; 1. The burn is acute skin injury caused by fire, hot water, steam, hot oil, sour and salty. 2. The burn cause blisters, irritability and restlessness, nausea, dryness of mouth, constipation, in case of serious, coma, dyspnea and death. The early stage of the burn, blisters form by skin damage and they burst into skin ulceration from which pus issues, the latter term, the wound form scab and healed up. 3. In a light case, medical treatment of the burn was used external treatment by medicine for externalism use, in a serious case, it was used both as an internal remedy and medicine for outward application. Also in the early stage, it was careful of using the cold and cool medicine, as the process of healing, it was used alleviating pain, detoxicating, moistening the skin, growing muscle and skin, convergence, evacuating pus, regeneration of the tissue, strengthen the spleen and nourishing the stomach. 4. The external treatment medication is Herba Ephedrae Oil(麻油), Radix ET Rhizoma Rhei(大黃), Glauberitum(寒水石), Water(水), Pig OiI(猪油), Pig Fat(猪脂), Radix Angelicae Gigantis(當歸), Rhizoma Coptidis(黃連), Cortex Phellodindri(黃栢). The White of an Egg(鷄子淸), Raw Honey(生蜜), Honey(蜜), Wine(酒), Etc. It is mostly the cold and cool medications. 5. Soft extracted and powered dosage form in external treatment is much used. The soft extracted form(32times used) are mostly Chung Ryang paste(淸凉膏) and Fructus Papaveris paste(罌粟膏). The powered form(30times used) are mostly Bingsang Powder(氷霜散), Bosaenggugo Powder(保生救苦散), Sahwang Powder(四黃散). The others is much a various powder adding solvent. 6. If varicella stage, erosion after varicella stage, oozing stage and extreme pain stage, the powder adding solvent is much used. If little oozing stage. ulcering stage, scabing stage and a chronic stage, Soft extracted dosage form is much used. 7. The most many(26.65%) used method is that apply each medication power mixed water(水), wine(酒), honey(蜜) in a wounded part.

• Journal of Periodontal and Implant Science
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• 제26권2호
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• pp.429-447
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• 1996

Transforming growth factor $-{\beta}$ is one of the polypeptide growth factors that mediate the activity of mesenchymal cells and regulate wound healing process via cell proliferation, migration and extracellular matrix formation. The purposes of this study is to evaluate the effects of transforming growth factor $-{\beta}$ on the protein synthetic activity of human periodontal ligament cells and human gingival fibroblasts. The cells which were prepared were primary cultured gingival fibroblasts and periodontal ligament cells from humans, and the fourth or sixth subpassage were used in the experiments. Cells were seeded and at a confluent state, 0, 0.5, I, 2.5, 5, 10 ng/ml $TGF-{\beta}$ and $2{\mu]Ci/ml\;[^3H]$ proline were added to the cells and cultured for 24 hours. Then, 1 and 5 ng/ml concentrations were selected and added to confluent cells and cultured for 24 and 48 hours. They were labeled with $2{\mu}Ci/ml\;[^3H]$ proline for 24 hours and a collagen assay was done by the Peterkofsky and Diegelman method. The results were presented as the mean disintegration per minute (dpm) per well and S.D. of four determinations, The results were as follows. : The total protein, collagen and noncollagenous protein synthesis in periodontal ligament cells and gingival fibroblasts were increased dose- dependently by transforming growth factor-p to 2.5-5 ng/ml concentration and decreased at 10 ng/ml concentration. The percent of collagen was slightly changed according to the concentration of transforming growth factor-po The effect of transforming growth $factor-{\beta}$ was not specific for collagen synthesis since it increased the total, noncollagenous and collagenous protein, simultaneously. In the comparison of protein synthetic activity between the human periodontal ligament cells and human gingival fibroblasts, the human gingival fibroblasts had higher activities than the human periodontal ligament cells at all times and concentrations of $TGF-{\beta}$. In the comparison of protein synthetic activity between the 24 hour effect and the 48 hour effect of $TGF-{\beta}$, the 48 hour cultured cells' synthetic activity decreased more than the 24 hour cultured cells at human periodontal ligament cells and human gingival fibroblasts. In conclusion, $TGF-{\beta}$ has important roles in the stimulation of protein synthesis in human periodontal ligament cells and human gingival fibroblasts. Thus, it may be useful for clinical application in periodontal regenerative procedures.

• The Korean Journal of Physiology and Pharmacology
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• 제23권2호
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• pp.151-159
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• 2019

Pruritus (itching) is classically defined as an unpleasant cutaneous sensation that leads to scratching behavior. Although the scientific criteria of classification for pruritic diseases are not clear, it can be divided as acute or chronic by duration of symptoms. In this study, we investigated whether skin injury caused by chemical (contact hypersensitivity, CHS) or physical (skin-scratching stimulation, SSS) stimuli causes initial pruritus and analyzed gene expression profiles systemically to determine how changes in skin gene expression in the affected area are related to itching. In both CHS and SSS, we ranked the Gene Ontology Biological Process terms that are generally associated with changes. The factors associated with upregulation were keratinization, inflammatory response and neutrophil chemotaxis. The Kyoto Encyclopedia of Genes and Genomes pathway shows the difference of immune system, cell growth and death, signaling molecules and interactions, and signal transduction pathways. Il1a, Il1b and Il22 were upregulated in the CHS, and Tnf, Tnfrsf1b, Il1b, Il1r1 and Il6 were upregulated in the SSS. Trpc1 channel genes were observed in representative itching-related candidate genes. By comparing and analyzing RNA-sequencing data obtained from the skin tissue of each animal model in these characteristic stages, it is possible to find useful diagnostic markers for the treatment of itching, to diagnose itching causes and to apply customized treatment.

• 생명과학회지
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• 제25권5호
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• pp.523-532
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• 2015

맥문동(Liriope platyphylla)은 염증(inflammation), 당뇨(diabetes), 신경퇴행성질환(neurodegenerative disorder), 비만(obesity), 변비(constipation), 아토피질환(atopic dermatitis) 등의 다양한 임상질환에 매우 우수한 치료효과를 나타내는 것으로 알려져왔다. 또한, 하이드로콜로이드막(hydrocolloid membranes, HCM)은 피부경화증 피부궤양(scleroderma skin ulcers), 피부궤양(cutaneous ulcers), 영구적 고막천공(permanent tympanic membrane perforations), 욕창(pressure sores), 욕창궤양(decubitus ulcers)과 같은 피부질환 치료에 많이 사용되고 있다. 따라서 본 연구에서는 기능성이 우수한 맥문동 추출물을 HCM에 혼합하여 맥문동 혼합 하이드로콜로이드막(HCM-LP)을 제조하고, 물리화학적 특성을 분석한 뒤 2도 화상을 유발한 SD 랫드에 14일 동안 처리하여 치료효과를 분석하였다. 그 결과, 일반 하이드로콜로이드막(HCM)에 비하여 HCM-LP에서 인장강도와 흡수성은 각각 38.4%, 46.3% 감소하였으나 표면거칠기는 38.1% 증가하였다. 화상을 유발한 SD 랫드에서 HCM-LP를 처리한 결과, 화상 유발 14일 후에 HCM-LP처리그룹은 GZ처리그룹에 비하여 유의적으로 화상크기 감소를 나타내었을 뿐만 아니라 흉터감소, 재상피화, 신생혈관형성 그리고 세포외기질의 침적을 유도하였다. 따라서 이러한 결과는 HCM-LP가 신생혈관형성과 연결조직형성 조절을 통해 SD 랫드에서의 화상 치료를 향상시킴을 의미한다. 또한, 본 연구는 HCM-LP가 피부상처의 치료에 적용할 수 있는 다른 기능성 물질을 포함하는 HCM의 개발에 대한 가능성을 제시하고 있다.

• Journal of Plant Biotechnology
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• 제50권
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• pp.1-10
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• 2023

고구마(Ipomoea batatas L. Lam)는 영양소, 가공 식품, 동물 사료 및 색소 재료의 유용한 공급원으로 이용 가능한 경제적으로 중요한 대표적인 뿌리 작물이다. 일반적으로 고구마의 저장 뿌리는 수확 후 저장 기간 동안 다양한 미생물과 질병에 의한 부패에 노출되기 쉽다. 수확 후 큐어링은 저장기간 동안 상처를 치유하고 미생물에 의한 부패를 방지하기 위한 가장 적합한 수단으로 알려져 있다. 본 연구에서는 큐어링과 연관된 분자적 기작에 관여하는 단백질들을 확인하기 위해, 큐어링 처리 후 저장기간 동안 단백질체의 변화를 분석하였다. 33℃ (큐어링) 및 15℃ (대조군)에서 3일 동안 처리하고 8주의 저장 기간이 지난 후 2D 전기영동 분석을 통해 단백질 spot의 변화를 확인한 결과, 31개 단백질 spot의 발현량이 차이 나는 것을 확인하였으며, 이들 중 15개의 단백질 spot을 동정하여 그 특성을 분석하였다. 동정된 단백질 중 alphaamylase (spot 1)는 큐어링 처리구에서만 발현량이 증가하였으며, probable aldo-keto reductase 2-like (spot 3) 및 hypothetical protein CHGG_01724 (spot 4)는 큐어링 및 대조구에서 동시에 발현량이 증가하였으나, sporamin A (spot 10)는 큐어링 및 대조구에서 발현량이 감소하였다. 한편, 대조구에서 enolase (spot 14)는 발현량이 증가하였으나, chain A of actinidin-E-64 complex+ (spot 19), ascorbate peroxidase (spot 22) 및 여러 sporamin 단백질들(spot 20, 21, 23, 24, 27, 29, 30 및 31)은 발현량이 감소하였다. 본 연구의 결과는 고구마 저장 뿌리에서 큐어링 처리와 관련된 단백질의 동정 및 수확 후 저장 기간동안 병 저항성과 관련된 기작에 대한 이해를 높이며, 향후 저온 저장 능력이 향상된 신품종 개발을 위한 후보 유전자의 도출에도 기여할 수 있을 것이다.

• 한국미생물·생명공학회지
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• 제38권4호
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• pp.420-427
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• 2010

바이러스 안전성이 보증된 무세포 소 양막 생물창상피복재 제조공정을 확립하고자 하였다. 기질세포를 제거하기 위해 효소(트립신)를 처리하는 공정과 바이러스를 불활화하기 위해 70% 에탄올, 0.05% sodium hypochlorite, 25 kGy 감마선 처리 공정을 포함하는 무세포 소 양막 제조공정을 확립하였다. 무세포 소 양막의 조직학적 분석과 전자현미경 분석 결과 면역거부반응을 일으킬 수 있는 상피층과 기질세포들이 잘 제거되었으며, 소 양막 콜라겐 섬유의 3차원적 구조가 잘 유지되어 있음을 확인하였다. 또한 상처치유효과가 있는 EGF, KGF, FGF와 같은 성장인자를 포함하고 있었다. 바이러스 불활화 효과를 검증하기 위해 국제적 가이드에 따라 4종의 바이러스(BHV, BVDV, BPIV-3, BPV)를 생물학적 지표로 사용하여 소 양막에 각 생물학적 지표를 첨가한 후각 바이러스 불활화 공정을 실시한 다음 각 바이러스를 회수하여 정량한 후 불활화 정도를 비교하였다. 24시간 70% 에탄올 처리 공정에서 BHV, BVDV, BPIV-3, BPV 모두 처리 시간 1시간 안에 검출한계 이하로 완벽하게 불활화되었다. 30분 0.05% sodium hypochlorite 처리 공정에서 BHV, BVDV, BPIV-3 같은 외피 바이러스는 BPV 같은 비-외피 바이러스에 비해 효과적으로 불활화되었다. 25 kGy 감마선 조사에 의해 BHV, BVDV, BPIV-3는 검출한계 이하로 완벽하게 불활화되었고, BPV도 효과적으로 불활화되었다. 3가지 바이러스 불활화 공정에서 BHV, BVDV, BPIV-3, BPV에 대한 log 바이러스 감소인수 합은 각각 ${\geq}$13.30, ${\geq}$14.32, ${\geq}$15.22, ${\geq}$7.57이었다. 이와 같은 결과 본 연구를 통해 확립된 무세포 소 양막 제조공정은 바이러스 안전성을 보증할 수 있는 충분한 바이러스 불활화 능력을 갖고 있는 것으로 판단된다.

• Journal of Periodontal and Implant Science
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• 제24권2호
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• pp.303-320
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• 1994

치주조직의 재생을 위하여 다양한 방법이 제시되어 왔으나 최근에는 치주인대세포를 선택적으로 유도하여 증식시키는 방법으로 성장인자에 대한 연구가 진행되고 있다. 중배엽세포를 조절하는 인자 중의 하나인 혈소판유래성장인자(Platelet-Derived Growth Factor, 이하 PDGF-AA, BB로 표기)는 폴리펩타이드계 성장인자로써 다양한 세포들에 대해 증식, 이주 및 기질합성에 촉진효과가 있다고 보고된 바 있다. 본 연구는 배양된 치주인대세포에 혈소판유 래성장인자를 농도별로 주입해서 세포의 증식능, 단백질 및 교원질 합성능을 측정해 보고, 골형성세포로의 분화에 대한 표식인자로 알칼린인산효소활성도를 알아보므로서 혈소판유래성장인자가 치주인대세포에 미치는 영향을 규명하고자 하였다. 교정치료를 위해 내원한 환자로 부터 건강한 제일소구치를 발거하여 치주인대세포를 분리, 배양하여 PDGF-AA, BB를 주입시키지 않은 군을 대조군으로 하고, PDGF -AA, BB를 각각 0.1, 1, 10, 100ng/ml로 주입시킨 군을 실험군으로하여 DNA 합성능, 총단백질과 교원질 합성능 및 알칼린인산효소활성도를 측정하여 다음과 같은 결과를 얻었다. DNA 합성능에 미치는 PDGF -AA, BB의 효과는 양군 공히 농도가 증가함에 따라 증가하는 경향을 보였으나 100ng/ml의 PDGF-BB를 투여한 군에서는 대조군과 유사한 정도를 나타내었다. 치주인대세포의 총단백질 합성양에 미치는 PDGF -AA, BB의 효과는 PDGF-AA, BB투여군 공히 농도가 증가함에 따라 총단백질 합성양이 증가하는 경향을 보였으며, 총단백질 합성양에 대한 PDGF-BB의 효과가 PDGF-AA보다 100ng/ml 투여군에서 현저히 높게 나타났다. 총단백질을 교원질(collagenase digestible protein : CDP)과 비교원성 단백질(noncollagenous protein : NCP)로 분류하여 비교하였을때 PDGF-AA, BB 투여군 공히 농도가 증가함에 따라 비교원성 단백질 합성양과 교원질 합성양이증가하는 경향을 보였으며, 양군 모두에서 비교원성 단백질 합성양이 교원질 합성양보다 높게 나타났다. 총단백질에 대한 교원질의 상대적 비율은 양군 공히 농도가 증가함에 따라 감소하는 경향을 나타내므로써 PDGF-AA, BB는 교원질에 특이하게 합성을 증가시키는 효과는 없음올 나타내었다. 알칼린인산효소활성도는 7, 14일째에서 PDGF-AA, BB 투여군 모두 대조군과 별 차이를 보이지 않았다.

• Journal of Periodontal and Implant Science
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• 제27권4호
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• pp.785-804
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• 1997

It was well known that calcium sulfate was biocompatible, resorbed rapidly in the body, had potential as a good barrier membrane. Platelet-derived growth factor(PDGF) was one of polypeptide growth factor that had been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. The purpose of this study was to evaluate the effects of a combination of calcium sulfate and PDGF on periodontal ligament cells in vitro to use as a regeneration promoting agent of periodontal tissue. Human periodontal ligament cells were prepared from the premolar tooth extracted for the orthodontic treatment. Cells were cultured in ${\alpha}-MEM$ contained with 20% FBS, at the $37^{\circ}C$, 100% of humidity, 5% $Co_2$ incubator. Cells were inoculated and cultured into 96 well culture plate with $1{\times}10^4cells/well$ of ${\alpha}-MEM$ for 1 day. After discarding the medium, those cells were cultured in ${\alpha}-MEM$ contained with 10% FBS alone(control group), in calcium sulfate(calcium sulfate group), in calcium sulfate treated with 15ng/ml of PDGF-BB(calcium sulfate+PDGF group), in ${\alpha}-MEM$ contained with 10% FBS treated with 15ng/ml of PDGF-BB(PDGF group) for 1, 2, 3 day respectively. And then each group was characterized by examining of the cell counting, MTT assay, collagen synthesis. The results were as follows. 1. In the analysis of cell proliferation by cell counting, both calcium sulfate group and calcium sulfate plus PDGF group showed no stastically significant difference compared to control group, but there was stastically significant difference between PDGF group and calcium sulfate group at 1, 2 day(P<0.05). 2. In the analysis of cell proliferation by MTT assay in calcium sulfate extracts, both calcium sulfate group and calcium sulfate plus PDGF group showed no stastically significant difference compared to control group, but there was stastically significant difference between PDGF group and calcium sulfate group at 2, 3 day, and between calcium sulfate plus PDGF group and calcium sulfate group at 2 day(P