• Parasites, Hosts and Diseases
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• 제42권1호
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• pp.19-26
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• 2004

We investigated the induction of resistance to Clonorchis sinensis infection by prior infection in rat and hamster models. Animals were challenged with C. sinensis metacercariae, then treated with praziquantel and reinfected. Worm recovery rate in reinfected animals was used to estimate resistance to reinfection. The determined resistance rates to reinfection in rats and hamsters were 97.7% and 10.3%, respectively. In rats, cure from the primary infection of C. sinensis increased resistant to reinfection, and the greatert the worm burden and the longer the duration of primary infection, the higher was the resistance rate. For primary infection doses of 10, 40 and 100 metacercariae per rat, the resistance rates were 87.4%, 93.8% and 98.4%, respectively. The resistance rates in rats after 2 or 8-week primary infection were 78.7% and 95.3%, respectively. All worms recovered from reinfected rats were immature. When cured rats were administered with methylprednisolone, resistance to reinfection became impaired. These findings indicate that rats develop a high degree of resistance to reinfection by C. sinensis after cure. The growths and maturations of reinfected worms were also impaired.

• Parasites, Hosts and Diseases
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• 제30권2호
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• pp.141-146
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• 1992

Kato후층도말변법 (M.C.T.S.)과 Stoll 회석충란계산법(D.E.C.T)으로 회충, 괸충, 간흡 충란 양성자 197명에 대하여 충란 정량검사를 실시하고 그 결과를 비교하여 상관함수식을 도출하였다. M.C.T.S.법에 소요된 검경시간은 표본 1매당 평균 12.6분으로 D.E.C.T.법의 14.6분보다 짧았으며, 층계부하가 낮은 감염자에 있어서도 위음성이 적었다. 각 윤충류 충란에 있어서 M.C.T.S.으로 얻은 결과를 대변 1 g당 충란수(E.P.G.)로 바꾸어 주는 전환함수식은 회충의 경우 E.P.G.=47.86×100.87 logM.C.T.S., 편충은 E.P.G.=41.69×100.83 logM.C.T.S., 간흡충은 E.P.G=63.10×100.55 logM.C.T.S.이었다. 장내 윤충류 감염에 대한 정량적 대변검사법으로 Kato후층도말변법이 Stoll희석충란계산 법보다 더 유용하다고 생각되었다.

• Parasites, Hosts and Diseases
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• 제45권2호
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• pp.157-161
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• 2007

A total of 1,496 rodents and insectivores were live-trapped at Yeoncheon-gun (n = 351), Paju-shi (804), and Pocheon-gun (343), Gyeonggi-do (Province), and examined for intestinal helminths, including Neodiplostomum seoulense, seasonally from December 2004 to September 2005. Six species of rodents, including Apodemus agrarius (1,366), Mus musculus (32), Micronytus fortis (28), Eothenomys regulus (9), Micronys minutus (6), and Cricetulus triton (3), and 1 species of insectivores Crocidura lasiura (54) were collected. A total of 321 adult N. seoulense were collected from 19 (1.4%) A. agrarius. The worm burden ranged from 1 to 101 per A. agrarius (mean; 16.9). No N. seoulense was observed in other rodent or insectivore species examined. The infection rate during autumn (4.5%) was higher than those during spring (0.8%), summer (0.8%), and winter (0.5%). The average number of N. seoulense in infected A. agrarius was the highest in spring (66.0 specimens), followed by autumn (15.2), winter (4.5), and summer (3.3). This study first confirms that A. agrarius is a natural definitive host for N. seoulense, and demonstrates that the infection rates and intensities vary seasonally and geographically.

• Journal of Animal Science and Technology
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• 제63권3호
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• pp.545-562
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• 2021

A 3 yr experiment was conducted to evaluate the influence of diet and feeding location on animal performance, carcass characteristics, whole blood counts, and internal parasite burden of lambs assigned to 1 of 4 treatments: 1) confinement fed 71% alfalfa, 18% barley pellet, 5% molasses, 0.013% Bovatec, 6.1% vitamin/mineral package diet (CALF), 2) confinement fed 60% barley, 26% alfalfa pellet, 4% molasses, 2.5% soybean-hi pro, 0.016% Bovatec, 7.4% vitamin/mineral package diet (CBAR), 3) field fed 71% alfalfa, 18% barley pellet, 5% molasses, 0.013% Bovatec, 6.1% vitamin/mineral package diet (FALF), and 4) field fed 60% barley, 26% alfalfa pellet, 4% molasses, 2.5% soybean-hi pro, 0.016% Bovatec, 7.4% vitamin/mineral package diet (FBAR). A year × location interaction was detected for ending body weight (BW), average daily gain (ADG), and dry matter intake (DMI); therefore results are presented by year. In all years, cost of gain and DMI were greater for CALF and FALF than for CBAR and FBAR feed treatments (p ≤ 0.03). In yr 2 and 3 field treatments had greater ending BW and ADG than confinement treatments. For all years, diet did not affect ending BW or ADG. In yr 1 dressing percent and rib eye area were greater for field finished lambs than confinement finished (p ≤ 0.02) and Warner-Bratzler shear force was greater for CALF and FALF (p = 0.03). In yr 2 lambs in FALF and FBAR treatments had greater leg scores and conformation than CALF and CBAR (p = 0.09). In yr 1, FALF had a greater small intestine total worm count than all other treatments. In yr 1, ending Trichostrongyle type egg counts were greater for FALF (p = 0.05). In yr 2, ending Nematodirus spp. egg counts were greater for FALF and lowest for CBAR (p < 0.01). Abomasum Teladorsagia circumcinta worm burden was greater in CALF than all other treatments (p = 0.07) in yr 2. While field finishing lambs with a grain- or forage-based diet we conclude that it is possible to produce a quality lamb product without adverse effects to animal performance, carcass quality or increasing parasite burdens.

• 대한약리학회지
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• 제10권2호
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• pp.25-29
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• 1974

The present clinical trial was performed to evaluate the anthelminthic effect and tolerance of Oxantel pamoate, a new antiwhipworm agent, in the treatment of T. trichiurus infection. Oxantel pamoate oral suspension was administered as a sing1e dose of 10 mg per kg. body weight to a total of 46 T. trichiurus infected cases selected out of 64 positives, by examining 89 fecal specimens collected from the soldiers and in-patients of xx Armed Forces General Hospital. For the evaluation, the negative conversion rate (cure rate) and the egg reduction rate were assessed 22 days after the treatment. The result obtained were as follows: 1. The prevalence rate of T. trichiurus infection in this study was 71.9%, while the mean E.P.G. of 46 treated cases was 126. 2. The egg negative conversion rate was 91.3%, and 98.4% of egg reduction rate was obtained. 3. From the observation of 107 worms expelled during 3 consecutive days after the treatment, it was known that sex ratio of male and female was about 1 : 2 and that average worm burden per capita was 2.3. 4. In all treated cases, the medication was readily accepted and well tolerated. On the hemograms, urinalysis and liver function tests, no significant differences were noted before and after the drug administration. There was also no detectable objective and subjective side effect.

• Asian-Australasian Journal of Animal Sciences
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• 제31권2호
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• pp.208-217
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• 2018

Objective: Examine the effects of supplementing bahiagrass hay (BG) with potentially anthelmintic quantities of hays of perennial peanut (PEA) or sericea lespedeza (LES) or seeds of velvet bean (Mucuna pruriens L.; MUC) or papaya (PAP) on the intake and nutritive value (Experiment 1), and the performance and parasite burden (Experiment 2) of goats. Methods: In Experiment 1, 38 male goats ($27.4{\pm}5.7kg$ body weight) were randomly assigned to each of 5 treatments: i) BG alone and BG plus; ii) PEA; iii) LES; iv) MUC; and v) PAP. Goats were fed for ad libitum consumption and adapted to the diets for 14 d followed by 7 d of measurement. The PEA, LES, MUC (50%, 50%, and 10% of the diet dry matter [DM], respectively), and PAP (forced-fed at 10 g/d) were fed at rates that would elicit anthelmintic effects. In Experiment 2, goats remained in the same treatments but were allocated to 15 pens (3 pens per treatment) from d 22 to 63. All goats were infected with parasites by grazing an infected bahiagrass pasture from 0800 to 1500 h daily and then returned to the pens. Results: Dry matter intake tended to be greater in goats fed PEA and LES than those fed BG (757 and 745 vs 612 g/d, respectively). Digestibility of DM (59.5% vs 54.9%) and organic matter (60.8% vs 56.0%) were greater in goats fed MUC vs BG, respectively. In Experiment 2, feeding PAP, LES, and PEA to goats reduced nematode fecal egg counts by 72%, 52%, and 32%, reduced abomasal adult worm counts by 78%, 52%, and 42%, and decreased plasma haptoglobin concentrations by 42%, 40%, and 45% relative to feeding BG alone, respectively. Conclusion: Supplementation with PEA, LES, and PAP decreased the parasite burden of goats but did not increase their performance. PAP was the most effective anthelmintic supplement.

• Archives of Pharmacal Research
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• 제28권9호
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• pp.1002-1012
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• 2005

5-Substituted 4-oxo-2-thioxo-1,,2,3,4-tetrahydropyrimidine were synthesized by interaction of 4­oxo-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-sulfonylhydrazide with some aldehydes to give the corresponding Schiff-bases, which after cyclization gave corresponding thiazolidinones. For some of the thiazolidinones, Mannich bases reaction was carried out. All the derivatives were tested for their possible inhibitory effect on Schistosoma mansoni cercarial elastase (CE). Only, N'-(4-methylbenzyledine)-4-oxo-2-thioxo-1,2 ,3,4-tetrahydropyrimidine-5-sulfonylhydrazide was found to have potent inhibitory effect on the CE activity with $IC_{50} = 264{\mu}M.$ Upon its use as a paint for mice tails before infection with S. mansoni cercariae, the compound formulated in jojoba oil caused a significant reduction ($93\%$; P-value = 0.0002) in the worm burden. IgG & IgM in mice sera were measured by using several S. mansoni antigens by ELISA. Sera from treated infected mice (TIM) 2, 4, and 6 weeks (W) post infection (PI) showed 1.2 folds lower, 1.2 folds higher, 1.7 folds lower IgM reactivity against soluble cercarial antigenic preparation (CAP), respectively, when compared with sera collected from infected untreated mice (IUM). Sera from TIM 2, 4, and 6WPI showed 1.3, 1.6, and 1.7 folds higher IgG reactivity, respectively against CAP than the IgG reactivity from IUM. Sera from TIM 2, 4 and 6WPI showed 1.5, 1.2 folds lower and 1.4 folds higher IgM reactivity, respectively against soluble worm antigenic preparation (SWAP) when compared with sera collected from IUM. Sera from TIM 2, 4, and 6WPI showed 1.4, 1 folds lower and 1 fold higher IgG reactivity, respectivley to SWAP when compared with sera from IUM. Sera from TIM 2, 4, and 6WPI had generaly lower IgM and IgG reactivities against soluble egg antigen (SEA) when compared with sera from IUM.

• Parasites, Hosts and Diseases
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• 제46권4호
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• pp.289-291
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• 2008

The Tamjin River which flows from Jangheung-gun via Gangjin-gun to the South Sea was reported to be a highly endemic area of Metagonimus yokogawai infection in 1977 and 1985. However, there were no recent studies demonstrating how much change occurred in the endemicity, in terms of prevalence and worm burden, of metagonimiasis in this river basin. Thus, a small-scale epidemiological survey was carried out on some residents along the Tamjin River basin in order to determine the current status of M. yokogawai infection. A total of 48 fecal samples were collected and examined by the Kato-Katz thick smear and formalin-ether sedimentation techniques. The egg positive rate of all helminths was 50.0%, and that of M. yokogawai was 37.5%, followed by C. sinensis 22.9% and G. seoi 4.2%. To obtain the adult flukes of M. yokogawai, 6 egg positive cases were treated with praziquantel 10 mg/kg in a single dose and purged with magnesium sulfate. A total of 5,225 adult flukes (average 871 specimens per person) of M. yokogawai were collected from their diarrheic stools. Compared with the data reported in 1977 and 1985, the individual worm burdens appeared to have decreased remarkably, although the prevalence did not decrease at all. It is suggested that the endemicity of M. yokogawai infection along the Tamjin River has been reduced. To confirm this suggestion, the status of infection in snail and fish intermediate hosts should be investigated.

• 정보처리학회논문지C
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• 제15C권5호
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• pp.351-358
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• 2008

DoS/DDoS로 대표되는 트래픽 폭주 공격은 대상 시스템뿐만 아니라 네트워크 대역폭 및 프로세서 처리능력, 시스템 자원 등을 고갈시킴으로써 네트워크에 심각한 장애를 유발하기 때문에, 신속한 트래픽 폭주 공격의 탐지는 안정적인 서비스의 제공 및 시스템의 운영에 필수요건이다. 전통적인 패킷 수집을 통한 DoS/DDoS의 탐지방법은 공격에 대한 상세한 분석은 가능하나 설치의 확장성 부족, 고가의 고성능 분석시스템의 요구, 신속한 탐지를 보장하지 못하는 문제점을 갖고 있다. 본 논문에서는 MIB 정보 갱신 시점 단위로 수집된 SNMP MIB 객체 정보를 바탕으로 Support Vector Data Description(SVDD)을 이용하여 보다 빠르고 정확한 침입탐지와 쉬운 확장성, 저비용탐지 및 정확한 공격유형별 분류를 가능케 하는 새로운 시스템을 설계 및 구현하였다. 실험을 통하여 만족스러운 침입 탐지율과 안전한 False Negative Rate(FNR), 공격유형별 분류율 수치 등을 확인함으로써 제안된 시스템의 성능을 검증하였다.

• Parasites, Hosts and Diseases
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• 제38권4호
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• pp.279-281
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• 2000

Pre-school children were examined for the presence of Enterobius vermicularis egg by perianal swab method. We visited 67 nurseries and 25 kindergartens between January, 1999 and April, 1999 in Chunchon, Korea. Of the 4,711 children examined, 434 (9.2%) were found to be positive for E. vermicularis egg. The egg positive rates of boys and girls were 10.1% and 8.1%, respectively. The rates were 7.8% in kindergartens and 9.7% in nurseries. Positive rate of 50 institutions was less than 10%. Rate in 35 institutions was from 10% to less than 20%. Rate in 7 institutions was equal to or over 20%. Out of 1,113 children examined twice, 28 (2.5%) children were positive consecutively, 53 (4.8%) were positive with negative conversion, 47 (4.2%) were negative with positive conversion, and 985 (88.5%) were consecutively negative. It means that in the low endemic area of enterobiasis with around 10% positive rate, the two consecutive examinations may increase the egg detection rate of 4.2-4.8%. The small number of consecutive egg positive children also suggests that the worm burden of the positive children might be low. Since the egg positive rate of that a9e group in this City increased from 1.85% (1997), and 3.0% (1998), the more intensive regular control should be executed.