• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2009년도 춘계학술대회 논문집
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• pp.652-655
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• 2009

The bimodal porous carbons were synthesized by using imprinting method with templates of SBA-15 particle and silica sphere and applied as supporting materials for the electro-catalyst of polymer electrolyte fuel cell (PEFC). The silica spheres with diameter size of 100 nm and SBA-15 particle having 200 nm -250 nm diameter and 700 nm -900 nm length were synthesized in this work. The bimodal porous carbons (S100) were prepared by using the silica spheres and SBA-15 as templates and mesophase pitch as a carbon source. The PtRu nanoparticle of ca. 1.9 nm were supported on the bimodal porous carbon support and the resulting PtRu/S100 catalysts was tested by the cyclic voltammetry. The use of bimodal porous carbon showed in comparable electro-catalytic activities with commercial catalyst. Though unclear effects of bimodal porosity of supports could be obtained in the scope of this study, morphological advantage in electrical conductivity can be considered on the electro-catalytic activity.

• Journal of Microbiology and Biotechnology
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• 제25권10호
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• pp.1714-1727
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• 2015

This work describes a new strategy for optimal design of Multiplex-PCR primer sequences. The process is based on the Particle Swarm Optimization-Simplex algorithm (Mult-PSOS). Diverging from previous solutions centered on heuristic tools, the Mult-PSOS is selfconfigured because it does not require the definition of the algorithm's initial search parameters. The successful performance of this method was validated in vitro using Multiplex-PCR assays. For this validation, seven gene sequences of the most prevalent bacteria implicated in urinary tract infections were taken as DNA targets. The in vitro tests confirmed the good performance of the Mult-PSOS, with respect to infectious disease diagnosis, in the rapid and efficient selection of the optimal oligonucleotide sequences for Multiplex-PCRs. The predicted sequences allowed the adequate amplification of all amplicons in a single step (with the correct amount of DNA template and primers), reducing significantly the need for trial and error experiments. In addition, owing to its independence from the initial selection of the heuristic constants, the Mult-PSOS can be employed by non-expert users in computational techniques or in primer design problems.

• Journal of Electrochemical Science and Technology
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• 제2권2호
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• pp.124-129
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• 2011

A simple process of fabricating micropillar structure and its influence upon enhancing electrochemical biosensor response were studied in this work. Conducting polymer PEDOT was used as a base material in formulating a composite with PVA. Micro porous PC membrane filter was used as a template for the micropillar of the composite on ITO electrode. This structure could provide plenty of encapsulating space for enzyme species. After dosing enzyme solution into this space, Nafion film tent was cast over the pillar structure to complete the micropillar cavity structure. In this way, the encapsulation of enzyme could be accomplished without any chemical modification. The amount of enzyme species was easily controllable by varying the concentration of the dosing solution. The more amount of enzyme is stored in the sensor, the higher the electrochemical response is produced. One more reason for the sensitivity improvement comes from the large surface area of the micropillar structure. Application of 0.7 V produced the best current response under the condition of pH 7.4. This biosensor showed linear response to the glucose in 0.1~1 mM range with the average sensitivity of $14.06{\mu}A/mMcm^2$. Detection limit was 0.01 mM based on S/N = 3.

• BMB Reports
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• 제40권3호
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• pp.444-447
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• 2007

Polymerase chain reaction (PCR) is a powerful technique in molecular biology and is widely used in various fields. By amplifying DNA fragments, PCR has facilitated gene cloning procedures, as well as molecular genotyping. However, the extraction of DNA from samples often acts as a limiting step of these reactions. In particular, the extraction of PCR-compatible genomic DNA from higher plants requires complicated processes and tedious work because plant cells have rigid cell walls and contain various endogenous PCR inhibitors, including polyphenolic compounds. We recently developed a novel solution, referred to as AnyDirect, which can amplify target DNA fragments directly from whole blood without the need for DNA extraction. Here, we developed a simple lysis system that could produce an appropriate template for direct PCR with AnyDirect PCR buffer, making possible the direct amplification of DNA fragments from plant leaves. Thus, our experimental procedure provides a simple, convenient, non-hazardous, inexpensive, and rapid process for the amplification of DNA from plant tissue.

• 한국멀티미디어학회논문지
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• 제19권4호
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• pp.747-755
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• 2016

Automated guided vehicles have been used for a long time to increase work efficiency in the logistics field, but it is difficult to apply to a variety of logistics sites due to either the restricted movement mechanism or expensive devices. In this paper, we present a flexible software architecture that is hardware-independent for a mobile power cart of the follow mode and implement it using a ROS software platform. Through the SCV analysis for the system functionalities, we design a package to track a user movement and a package to control a new hardware platform. It has an advantage to use a variety of movement algorithms and hardware platforms by applying the strategy pattern and the template method pattern for the design of a software architecture. Through the performance evaluation, we show that the proposed design is maintainable in terms of a software complexity and it detects a user's movement by obtaining a user skeleton information so that it can control a hardware platform to move at a certain distance.

• 한국정밀공학회지
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• 제16권4호통권97호
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• pp.37-45
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• 1999

Many automated welding equipment are used in the industry. However, there are some problems to get quality welds because of the geometric error, thermal distortion, and incorrect joint fit-up. These factors can make the gap between base plates in case of a thick plate welding. The welding product with the quality welds can not be obtained without consideration of the gap. In this paper, the robot path and welding conditions are modified to get the quality weld by detecting the position and size of the gap. In this work, a low-priced laser range sensor is used. The 3-dimensional information is obtained using the motion of a robot, which holds a laser range sensor. The position and size of the gap is calculated using signal processing of the measured 3-dimensional information of joint profile geometry. The data measured by a laser range sensor is segmented by an iterative end point method. The segmented data is optimized by the least square method. The existence of gap is detected by comparing the data with the segmented shape of template. The effects of robot measuring speed and gap size are also tested. The recognizability fo the gap is verified as good by comparing the real joint profile and the calculated joint profile using the signal processing.

• 대한조선학회 특별논문집
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• 대한조선학회 2007년도 특별논문집
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• pp.57-67
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• 2007

As the competition among commercial shipyards gets more intense and the number of ships to be designed is increasing significantly, the state is that engineering work volume has increased proportionately. Under these conditions, various attempts have been tried to improve performance in design activities and it has come to a vital issue as to how the design process should be managed and conducted in more efficient manner. To achieve this goal, an engineering process management system has been developed, named 'DSME Engineering Wizard System'. It aims to accelerate process performance by managing execution, promoting collaboration and maximizing engineering data reusability based on workflow concept. For the application of this system, Marketing Design which is one of the major processes for commercial ship design was analyzed and established into a unique workflow template consisting of activities, getting design experiences organized into a best practice in which engineering tasks are performed in the way proven most efficient.

• 한국컴퓨터정보학회논문지
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• 제13권3호
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• pp.1-13
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• 2008

Gestwicki 등은 객체 그래프의 더 좋은 그림을 얻기 위하여 클래스 다이어그램으로부터 객체 다이어그램의 구조를 탐지하고 예측하는 기술을 제안하였다. 그들의 방법은 클래스 다이어그램으로부터 두 가지 예측 가능한 구조-되부름 덩어리 (recursive cluster)와 잎 덩어리 (leaf cluster)를 찾는다. 하지만 이 방법은 출발점이 클래스 다이어그램이므로 실제 프로그램에 적용하기에는 실제적이지 않다. 본 연구는 자바 클래스 파일에 적용하기 위하여 그들의 방법을 확장하고 구현한다. 자바 클래스 파일로부터 추출한 클래스들과 덩어리들의 구조와 관련성을 얻는다. 이 정보는 JIVE와 같은 자바 프로그램 실행 시각화 도구에게 제공되어 객체 다이어그램의 틀로 사용될 수 있다.

• 제어로봇시스템학회논문지
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• 제22권11호
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• pp.895-903
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• 2016

In a fire disaster in a tunnel, people should be rescued immediately using the information obtained from cameras or sensors. However, in heavy smoke from a fire, people cannot be clearly identified by a mounted CCTV, which is only effective in a clear environment. A thermal camera can be an alternative to this in smoky situations and is capable of detecting people from their emitted thermal energy. On the other hand, the thermal image camera has a smaller field of view than an ordinary camera due to its lens characteristics and temperature error, etc. In order to cover a relatively wide area, panoramic image construction needs to be implemented. In this work, a template-based similarity matching algorithm for constructing the panorama image is proposed and its performance is verified through experiments. This scheme provides guidelines for coping with difficulty in image construction, which requires an exact correspondence search for two images in cases of heavy smoke.

• 한국정보통신학회논문지
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• 제8권6호
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• pp.1182-1187
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• 2004

본 논문에서는 실시간으로 눈과 눈썹주위의 특징을 추적하는 새로운 알고리즘을 제안하고자 한다. 제안된 알고리즘은 적외선 LED와 적외선카메라로 밝은 동공 효과를 만들어 동공을 추적 한 후, 템플릿은 얼굴 특징을 매개변수화 하기 위해, 동공 좌표는 각각의 프레임에서 눈과 눈썹 영상을 추출하기 위하여 사용한다. 또한, 템플릿 변수는 표본 영상을 가지고 학습하는 과정에서 구성한 PCA기저를 이용하여 추출된 영상을 PCA 분석하여 구한다. 제안된 시스템은 초당 30 프레임의 영상에서 초기 설정 및 교정 작업 없이 머리 움직임이 많거나 폐색이 있는 경우에도 견실하게 동작하였다.