• Title/Summary/Keyword: work plate

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Transdifferentiation of bovine epithelial cells towards adipocytes in the presence of myoepithelium

  • Sugathan, Subi;Lee, Sung-Jin;Shiwani, Supriya;Singh, Naresh Kumar
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.2
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    • pp.349-359
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    • 2020
  • Objective: Orchastric changes in the mammary glands are vital, especially during lactation. The secretary epithelial cells together with the supporting myoepithelial and stromal cells function cordially to secrete milk. Increase in the number of luminal epithelial cells and a decrease in adipocytes are visible during lactation, whereas the reverse happens in the involution. However, an early involution occurs if the epithelial cells transdifferentiate towards adipocytes during the lactation period. We aimed to inhibit the adipocyte transdifferentiation of luminal cells by restraining the peroxisomal proliferator-activated receptor γ (PPARγ) pathway. Methods: Linolenic acid (LA) and thiazolidinediones (TZDs) induced adipogenesis in mammary epithelial cells were conducted in monolayer, mixed culture as well as in transwell plate co-culture with mammary myoepithelial cells. Results: Co-culture with myoepithelial cells showed higher adipogenic gene expression in epithelial cells under LA+TZDs treatment. Increase in the expressions of PPARγ, CCAAT/enhancer-binding protein α and vimentin in both mRNA as well as protein levels were observed. Whereas, bisphenol A diglycidyl ether treatment blocked LA+TZDs induced adipogenesis, as it could not show a significant rise in adipose related markers. Although comparative results were found in both mixed culture and monolayer conditions, co-culture technic was found to work better than the others. Conclusion: Antagonizing PPARγ pathway in the presence of myoepithelial cells can significantly reduce the adipogenisis in epithelial cells, suggesting therapeutic inhibition of PPARγ can be considered to counter early involution or excessive adipogenesis in mammary epithelium in animals.

The design of 111m high steel towers with 220kv double circuits crossing 12 km wide Bangladesh River (230KV 2회선승 111M 높이 철탑설계 (I) (강폭 12km인 Bangladesh Jamana강 횡단용))

  • 이재숙
    • Journal of the Korean Professional Engineers Association
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    • v.15 no.4
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    • pp.12-24
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    • 1982
  • East Parts of Bangladesh have been benifited by low cost energy generated by domestic natural gas but West parts where energy generated by imported fuel. Bangladesh Government authority has very much concerned to transmit the low cost electricity to the West from the East for past several years. To solve such concerns, cross-country 230kv double circuits Power transmission line was proposed, however there was a big obstacle for the realization of this line to cross the Jamuna river which has 12 km long width with a deep muddy river bed. A consultant engineering firm named Merz-Mclellan anyway finalized this plan and a world-wide bid was announced on June 31, 1979. Due to the expected difficulty to construct the towers on sea like area, only three construction groups have participated. including a Korean joint venture organization of Samsung-Korean Developement corporation-Kolon Electric Machinery company. After 3 months bid evaluation, contract was awarded to Korean Consosium and KEM Co was in charge of designing steel towers with anchor bolts and base plates beside to electrical engineering field. Then KEM Co have faced and over-comed many unenpected technical difficulties such as forced eccentricity joint on base plate, distorsion issue of 60mm thick plates welding, threading anchor bolts, tad heat treatment of some anchor bolts, disagreement from Consultant Engineer on multiplying factor of leg stresses for 45$^{\circ}$ wind and on reducing O.L.F for wind loads on cables for such 1220km long spans. After spending two years long period for designing and engineering towers, base plates, and anchor bolts, first shipment of tower was finally realized on Nov. 8, 1981 and on the other hand KDD has proceeded concrete caisson work on schedule at Jamuna river site and expected to complete tower erection and stringing of cables within this year of 1982 which was original completion target.

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Cleanliness Test by Spray-Type Cleaning Agent for Electronic and Semiconductor Equipment (전자·반도체용 스프레이 분사형 세정제에 대한 청정도 평가)

  • Heo, Hyo Jung;Row, Kyung Ho
    • Korean Chemical Engineering Research
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    • v.47 no.6
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    • pp.688-694
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    • 2009
  • A spray-type cleaning agent in utilizing dust-remover on PCB was chosen to study the cleanliness test and efficiency. In order to choose alternative environmental-friendly cleaning agents, it is important that the systematic selection procedures should be introduced and applied through the evaluation of their cleaning ability, environmental characteristics, and economical factors, and that the objective and effective evaluation methods of cleanliness should be established for the industry. A novel cleaning evaluation method with scanning electron microscopy/energy-dispersive X-ray analysis of surface observation evaluation method and an infra-red thermography camera(THERMOVISION A20 model) was studied in this work. The sound card(CT-2770 model) cut by $2{\times}2cm$ size was used as a part, and before and after the spray cleaning, the cleanliness was observed by the image analyzer of SEM and further the removal efficiency of dust was quantitatively evaluated by the component analysis of EDX. For the parts of P4TE model motherboard and IPC-A-36 PCB plate, before and after the spray cleaning, temperature differences were measured and compared at room temperature and 50 oven temperature by an infra-red thermography camera in the contaminants of dust and iron powder.

Design of Heliostat Field for 200kW Tower Type Solar Thermal Power Plant (200kW 탑형 태양열발전시스템의 Heliostat Field 설계)

  • Park, Young Chil
    • Journal of the Korean Solar Energy Society
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    • v.32 no.5
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    • pp.41-51
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    • 2012
  • Heliostat field is the most important subsystem in the tower type solar thermal power plant since its optical performance affects the total system efficiency most significantly while the construction cost of it is the major part of total construction cost in such a power plant. Thus a well designed heliostat field to maximize the optical efficiency as well as to minimize the land usage is very important. This work presents methodology, procedures and result of heliostat filed design for 200kW solar thermal power plant built recently in Daegu, Korea. A $2{\times}2(m)$ rectangular shaped receiver located at 43(m) high and tilted $28^{\circ}$ toward heliostat field, 450 of heliostats of which the reflective surface is formed by 4 of $1{\times}1(m)$ flat plate mirror facet, and the land area having about $140{\times}120(m)$ size are used to form the heliostat field. A procedure to deploy 450 heliostats in radial staggered nonblocking formation is developed. Also the procedures to compute the cosine effect, intercept ratio, blocking and shading ratio in the field are developed. Finally the heliostat filed is designed by finding the optimal radial distance and azimuthal spacing in radial staggered nonblocking formation such that the designed heliostat field optical efficiency could be maximized. The designed heliostat field has 77% of annual average optical efficiency, which is obtained by annually averaging the optical efficiencies computed between the time of where sun elevation angle becomes $10^{\circ}$ after sunrise and the time of where sun elevation angle becomes $10^{\circ}$ before sunset in each day.

Study on the Performance of Laser Welded joint of Aluminum alloys for Car Body

  • Kutsuna, Muneharu;Kitamura, Shuhei;Shibata, Kimihiro;Salamoto, Hiroki;Tsushima, Kenji
    • Proceedings of the KWS Conference
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    • 2002.10a
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    • pp.620-625
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    • 2002
  • Considering the fuel consumption of car, a light structure of aluminum alloys is desired for car body nowadays. However, fusion welding of aluminum alloys has some problems of reduction of joint efficiency, porosity formation and hot cracking. ill the present work, investigation to improve the joint performance of laser welded joint has been carried out by addition of Cu, Ni, and Zr to A6N01 alloy welds. Aluminum alloy plate of 2.0mm in thickness with filler metal bar was welded by twin beam Nd:YAG laser facility (total power:5kW). The filler metals were prepared by changing the chemical compositions for adding the elements into the weld metal. Thirteen filler metal bars were prepared and pre-placed into the base metal before welding. Ar gas shielding with a flow rate of 10 l/min was used. The defocusing distance is kept at 0 mm. At travel speeds of 3 to 9 m/min and at laser power of 5kW (front beam 2kW rear beam 3kW), full penetration welds were obtained, whereas at travel speeds of 12 to 18 m/min and same power, partial penetration was observed. The joint efficiency of laser-welded joint was improved by the addition of Cu, Ni, and Zr due to the solid solution hardening, grain refining and precipitation hardening. The type of hardening has been further considered by metallurgical examination.

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[ $CO_2$ ] Laser Weldability and Formability of High Strength Steels for Tailored Blanks Applications (TWB용 고강도 박판 강재의 $CO_2$ 레이저 용접성 및 성형성)

  • Lee, Won-Beom;Park, Seong-Ho
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2004.05a
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    • pp.365-372
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    • 2004
  • The laser welding and its analysis were carried out using high power 6kW $CO_2$ laser for high strength steels such as DP, TRIP and conventional high strength steels. Bead on plate welding of thin sheet was examined to investigate the effect of weld variables of laser welding, and to obtain optimum welding condition. In order to investigate the formability of welded high strength steels, LDH test was added on this work. At high welding speed, the partial penetration was obtained by low heat input. Meanwhile, porosity was formed in the bead at low weld speed because of extremely higher heat input. The optimum welding condition of welding was derived from bead width, penetration and hardness property. It was shown that the DP steels had lower porosity level and smooth bead shape, therefore better laser weldability than TRIP steels and conventional high strength steels. In addition, LDH test shows that the welded DP steels have about $90\%$ formability value of base metal, although TRIP steel and conventional high strength steels have about $80\%$ formability value of its base metal.

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Germination Rate and Microbial Safety during Cultivation of Disinfected Seeds (새싹 종자 소독 여부에 따른 발아율과 재배기간별 미생물 오염도)

  • Park, Eun-Jung;Kwon, Joong-Ho;Lee, Yeon-Kyung
    • Food Science and Preservation
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    • v.16 no.2
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    • pp.292-298
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    • 2009
  • This study investigated the microbial safeties and germination rates of five domestic sprout species(alfalfa, broccoli, clover, red cabbage, and red radish) grown from disinfected seeds. The 48 h germination rates of all seeds were over 90%, regardless of treatment. Seed total plate count(TPC) and coliform levels were reduced significantly(p<0.05) by treatment with 20,000 ppm calcium hypochlorite solution at $25^{\circ}C$ for 15 min, following FDA recommendations. However, after germination, all sprouts regardless of treatment exhibited bacterial counts of $10^7-10^8CFU/g$. Listeria monocytogenes was detected at $10^3-10^4CFU/g$ on germinated non-disinfected clover seeds at days 1, 2, and 5. In conclusion, although sprout germination from disinfected seeds potentially permits the growth of sprouts with lower pathogen counts, there were no significant differences in TPC or coliform levels between sprouts grown from disinfected seeds and control sprouts. Further work is needed to improve the microbial safety of cultivated sprouts and to find optimal conditions for seed germination.

Hydrothermal synthesis of $(Li,Al)MnO_2(OH)_2$:Co compound (수열법에 의한 $(Li,Al)MnO_{2}(OH)_{2}$:Co 화합물의 합성)

  • 최종건;황완인;김판채
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.11 no.4
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    • pp.154-159
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    • 2001
  • (Li,Al)$MnO_2(OH)_2$:Co compound was synthesized by hydrothermal method. $MnO_2$, LiOH.$H_2$O, $Co_3O_4$ and $Al(OH)_3$ were used as starting materials and the optimum conditions for synthesis of monolithic (Li,Al)$MnO_2(OH)_2$:Co compound were as follows : reaction temperature; $200^{\circ}C$, reaction time; 3 days, hydrothermal solvent; 3M-KOH solution, reaction apparatus; seesaw type, atomic ratio of Li:Al:Mn;Co = 1:2.1:2.5~2:0.5~1. Monolithic(Li,Al)$MnO_2(HO)_2$:Co compound synthesized in this work had a god crystallinity and excellent color forming effect as a blue pigment compatible with natural mineral. The particles of the synthesized (Li,Al)$MnO_2(OH)_2$:Co compound have hexagonal plate shape with the size of 0.5~1 $\mu\textrm{m}$.

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Microbiological Hazard Analysis of Cooked Foods Donated to Foodbank (II) (푸드뱅크 기탁 조리식품의 미생물학적 위해분석 (II))

  • Park, Hyeong-Su;Ryu, Gyeong
    • Journal of the Korean Dietetic Association
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    • v.13 no.4
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    • pp.389-406
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    • 2007
  • This study was conducted to estimate the safety level of non-cooking and cooking processed foods to propose the sanitary management of foods donated to foodbanks. The time and temperature were measured and the microbial levels of aerobic plate counts (APC), coliforms, E. coli, Salmonella spp., S. aureus, B. cereus, and E. coli O157:H7 were analyzed on ten food items donated to seven foodbanks. The amount of cooked foods donated to each foodbank was about 10 to 40 servings. All foodbanks hired a supervisor and had at least one refrigerator/freezer and one temperature-controlled vehicle, but only four foodbanks had the separate offices to manage the foodbank operation. The flow of donated foods was gone through the steps; production, meal service and holding at donator, collection by foodbank, transport (or holding after transport) and distribution to recipients. After production, the levels of APC of both non-cooking and cooking processed foods were complied with the standards by Ministry of Education & Human Resources Development, and were not increased till distribution. Only the level of coliforms in dried squid & cucumber salad (1.5×$10^3$ CFU/g) was not met the standards. E. coli and other pathogens were not detected in all tested samples. The microbial levels of delivery vessels and work tables were satisfactory, but the APC levels of two of four tested serving tables (6.9×$10^3$ and 5.3×$10^3$ CFU/100$cm^2$) and the coliforms level of one (1.1×$10^3$ CFU/100$cm^2$) were over the standards. The air-borne microflora level in serving room was estimated as satisfactory. It took about 3.0 to 6.5 hours from after-production to distribution and the temperatures of donated foods were exposed mostly to temperature danger zone, which had a high potential of microbial growth. These results imply that a checklist to monitor time and temperature in each step should be provided and the employees involving foodbank operation should be properly educated to ensure the safety of donated foods.

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Separation of Phospholipids in Step-Gradient Mode (Setp-Gradient Mode를 이용한 인지질의 분리)

  • Lee, Ju Weon;Row, Kyung Ho
    • Applied Chemistry for Engineering
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    • v.8 no.4
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    • pp.694-699
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    • 1997
  • Normal-phase HPLC was used to separate the useful phospholipids, PE, PI and PC in soybean lecithin. The mobile phase used in this experiments were haxane, isopropanol and methanol, the gradient mode was applied as the three components could not be separated by the isocratic mode. To find the optimum separation condition, the concentration profiles of effluents were calculated from the plate theory and the capacity factor in step-gradient mode. From the calculated results, PE was separated with hexane/isopropanol/methanol = 90/5/5vol.% in isocratic mode and PI and PC were resolved in the conditions of 10min gradient time and the second mobile phase of hexane/isopropanol/methanol=50/20/30vol.% in step-gradient mode. The agreement between the calculated concentration profile and experimental data was good, so the methodology developed in this work can be used to obtain the optimum separation condition in gradient mode.

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