• Journal of the Korean Wood Science and Technology
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• v.33 no.4 s.132
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• pp.23-29
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• 2005

The fancy veneer flooring board is high value-added wooden material. The classification of the flooring boards by the observation with the naked eye causes color difference among fancy veneers. It turned out that this inaccuracy of color difference among fancy veneers increased in case of the identification by metamerism or the flooring construction. Therefore, this study was performed to classify fancy veneers produced from 3 species such as Tilia sp., Betula sp., and Acer sp. which were identified with the naked eye by Light and Dark grade, by using CIELAB. In addition, each specie's threshold on CIERGB was investigated and a range of hue, saturation and intensity by an application of HSI color model were studied. Intensity of the HIS color model could be the best value to be used when color, saturation and intensity of the HSI color model were used for the classification of the flooring board's color. In addition, it seemed that color range of all three criteria lied between $45^{\circ}$ and $55^{\circ}$. In the case of identification by threshold of RGB element, considering only specific color element value is unlikely to lead to accurate classification of fancy veneers of flooring board.

• Journal of the Korean Wood Science and Technology
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• v.52 no.4
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• pp.343-362
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• 2024

Eurycoma longifolia (pasak bumi) is a popular medicinal plant in Indonesia and is widely used in various products. Its high economic value has caused illegal harvesting and product falsification. Using molecular techniques, the authentication and traceability of E. longifolia derivatives can be controlled to ensure consumer safety. Therefore, this study aimed to authenticate the products and derivatives of E. longifolia (pasak bumi) produced, marketed, and consumed in Indonesia using molecular identification techniques. Genomic DNA from 37 leaf samples collected from the Sumatran mainland and the Riau Islands and six E. longifolia products were amplified and sequenced using trnL-trnF and internal transcribed spacer (ITS) regions. The results revealed that all leaf samples were indeed E. longifolia based on the markers used, with the six products, only the herbal tea product (sample code TCPB) was most likely derived from E. longifolia based on the two regions, suggesting that not all products labelled as E. longifolia in the market are authentic. The results also indicated that several other plants species are used as substitutes or adulterants, including Simaba spp., Simarouba spp., Homalolepis spp., Vernonia gigantea, Elephantopus scaber, Gymnanthemum amygdalinum, Cyanthillium spp., Potentilla lineata, Ailanthus altissima, Geijera paniculata, Hannoa chlorantha, and Dalbergia spp. Klebsiella pneumoniae bacteria were also identified in this study on the outer wooden cup of E. longifolia products. Therefore, this molecular approach is effective in identifying the authenticity of E. longifolia products, with trnL-trnF and ITS as the recommended DNA markers.

• Journal of Conservation Science
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• v.34 no.5
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• pp.359-368
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• 2018

The purpose of this study was to analyze the wood species of the stern plank and persimmon seed from Daebudo shipwreck No.2 excavated from Daebudo, Danwon-gu, Ansan-si, and Gyeonggi-do by wiggle-matching of radiocarbon measurements. Results of the analysis showed that all the wooden boards of the hull were made from Pinus Hard Pine Group. The other parts of hull and wooden pegs were made from Pinus Hard Pine Group, Castanea spp., Quercus spp. sect. Lepidobalanus, Alnus spp., and Zelkova serrata Makino. Excavated wide-tooth wooden comb and fine-tooth bamboo comb were made from Acer spp. and Bambusoideae. Excavated rope was made from Pueraria thunbergiana Benth. The stern plank, wooden support, and persimmon stone showed ${\pm}2{\sigma}$ radiocarbon date(95.4% confidence interval) of AD 1151-1224. This indicated that the Daebudo shipwreck No.2 occurred during the early or middle 12-13th century. Our radiocarbon dating results confirmed the date speculated by archeologists based on the ceramic style.

• Journal of Conservation Science
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• v.2 no.2 s.2
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• pp.3-14
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• 1993

This study was carried out to identify the species of wooden coffins excavated from ancient kings' tombs of Nakrang and Pro-Three-Kingdoms to the beginning period of the Three Kingdoms, and the private tombs of the middle stage at the Yi dynasty. The species of wooden coffins were identified as three softwoods(Thxus cuspidata, Sciodopitys verticillata, Pinus densiflora) and six hardswoods(Quercus acutissima, Quercus serrata, Zelkova serrata, Platycarya strobilarea, Alnus japonica and Morns spp.). Thxus cuspidata, wooden coffins of Nakrang kings' tombs, is distributed through Korea. However in previous examination it was known as Cunninghania lanceolata, native species in south China. Sciodopitys verticillata used as a wooden coffin of King Muryung's tomb was native to Japan. It was a material evidence proving the cultural exchange between Paekche Kingdoms and Japan in those days. Pinus densiflora was the species of wooden coffins excavated from private tombs in Yi dynasty, which was not found out from Pro-Three-Kingdoms and Three-Kingdoms. Quercus accutissima was used for making a trough shape wooden coffins at Daho-ri ancient tombs and Quercus serrata was used a outer wooden coffin of Nakrang kings' tombs. An adamant iron mongery might be used to manufacturing a trough shape wooden coffin. This indicates that a developed iron-manufacturing technics existed in Korea at that time. Zelkova serrata was used for making wooden coffins of Imdang and Kaya ancient tombs, Chunma and Hwangnam king tombs. Platycarya strobizacea was used for making wooden coffin of Daegok-ri, Alnus japonica for Daho-ri coffins and Morus spp. for Imdang coffins.

• Mycobiology
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• v.48 no.2
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• pp.95-103
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• 2020

The genus Termitomyces (Lyophyllaceae, Basidiomycota) is often associated with fungus-feeding termites (Macrotermitinae) due to their strong symbiotic relationships. The genus is widely found exclusively in certain regions of Africa and Asia. They are recognized as edible mushroom within Southeast Asia as well. But it is often misidentified based on morphology by the local communities especially in Malaysia for Chlorophyllum molybdites which is a highly poisonous mushroom. Thus, it is necessary to study the genus for Malaysia with the synergy of using both morphological and molecular identification. In this study, we aim to describe another new species as an addition to the genus Termitomyces found within Sabah, Malaysia. We generated two new sequences (nrLSU and mtSSU) for the new species and a total of 28 nrLSU and mtSSU sequences were retrieved from GenBank for the phylogenetic analysis using maximum likelihood and Bayesian inferences. We identified that the new collection from Sabah province is a new species and named as Termitomyces gilvus based on the termites found in the mound. A phylogeny tree made from the concatenated genes of LSU and mtSSU suggests that T. gilvus is closely related to T. bulborhizus from China. According to our results, the combination of molecular and morphology proved to be a robust approach to re-evaluate the taxonomic status of Termitomyces species in Malaysia. Additional surveys are needed to verify the species diversity and clarify their geographic distribution.

• Research in Plant Disease
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• v.30 no.1
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• pp.66-77
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• 2024

Black wood ear mushroom (Auricularia auricula-judae) is one of the most economically important mushrooms in China, Japan, and Korea. The cultivation of wood ear mushrooms on artificial substrates is more efficient in terms of time and cost compared with their natural growth on trees. However, if the substrate cultivation is infected by fast-growing fungi, the relatively slow-growing ear mushroom will be outcompeted, leading to economic losses. In this study, we investigated the competitive fungal isolates from substrates infected with fast-growing fungi for the cultivation of ear mushrooms in Jangheung and Sunchon, Korea. We collected 54 isolates and identified them by sequencing their internal transcribed spacer region with morphological identification. Among the isolates, the dominant isolates were Trichoderma spp. (92.6%), Penicillium spp. (5.6%), and Talaromyces sp. (1.8%). To find an appropriate eco-friendly biocontrol agent, we used five Streptomyces spp. and Benomyl, as controls against Trichoderma spp. and Penicillium spp. Among the six Streptomyces spp., Streptomyces sp. JC203-3 effectively controlled the fungi Trichoderma spp. and Penicillium spp., which pose a significant problem for the substrates of black wood ear mushrooms. This result indicated that this Streptomyces sp. JC203-3 can be used as biocontrol agents to protect against Trichoderma and Penicillium spp.

• Microbiology and Biotechnology Letters
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• v.46 no.3
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• pp.300-312
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• 2018

Whole-genome sequencing of the wood-rotting fungus, Flammulina fennae, was carried out to identify carbohydrate-active enzymes (CAZymes). De novo genome assembly (31 kmer) of short reads by next-generation sequencing revealed a total genome length of 32,423,623 base pairs (39% GC). A total of 11,591 gene models in the assembled genome sequence of F. fennae were predicted by ab initio gene prediction using the AUGUSTUS tool. In a genome-wide comparison, 6,715 orthologous groups shared at least one gene with F. fennae and 10,667 (92%) of 11,591 genes for F. fennae proteins had orthologs among the Dikarya. Additionally, F. fennae contained 23 species-specific genes, of which 16 were paralogous. CAZyme identification and annotation revealed 513 CAZymes, including 82 auxiliary activities, 220 glycoside hydrolases, 85 glycosyltransferases, 20 polysaccharide lyases, 57 carbohydrate esterases, and 45 carbohydrate binding-modules in the F. fennae genome. The genome information of F. fennae increases the understanding of this basidiomycete fungus. CAZyme gene information will be useful for detailed studies of lignocellulosic biomass degradation for biotechnological and industrial applications.

• The Korean Journal of Mycology
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• v.27 no.1 s.88
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• pp.68-72
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• 1999

Wood-rotting fungi of the Aphyllophorales were collected through field trips to mountain areas of the country from January to December of 1997. Through the observation and identification of specimens, two genera, Meruliopsis (type species=M. taxicola) and Pseudomerulius (type species=P. aureus), and four species, Phanerochaete calotricha, Phanerochaete chrysorhiza, Meruliopsis corium, and Pseudomerulius aureus were confirmed as new wood-rotting fungi to Korea and are registered here with descriptions.

• Mycobiology
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• v.41 no.4
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• pp.248-251
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• 2013

Ganoderma is a cosmopolitan wood-rot basidiomycete that has been extensively studied for its pathogencity and medicinal properties. Identification of Ganoderma based on macro-microscopic features led to large number of synonyms which resulted in 250 taxonomic names. A Ganoderma species collected from Courtallam, Tamil Nadu was identified as G. cupreum. Phylogenetic analysis inferred from internal transcribed spacer rDNA region resolved the Indian isolate MYC1 as Ganoderma cupreum which clustered with Australian and Asian "cupreum" clade with 85% bootstrap support BS and shared 99% and 98% nucleotide similarity with Malaysian and Australian 'cupreum' respectively. This study represents the first molecular evidence of G. cupreum from Asian origin.

• Journal of Conservation Science
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• v.33 no.1
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• pp.17-24
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• 2017

Studies were conducted on manufacturing techniques by applying microscopy and conservation treatments on the annals of Joseon dynasty storage box at the National Museum of Korea. The results revealed that lime tree wood(Tilla spp.) was used to make the annals of Joseon dynasty storage box. Lacquering techniques were used to coat the box with a layer of lacquer and bone ash and then cover it with traditional Korean paper. After being covered with traditional Korean paper, more layers were applied in the following sequence: mud ashes, black lacquer, pure lacquer, and black lacquer. Before conservation treatments, some components and lacquer layers were missing in addition wood joint were loose. Therefore, conservation and restoration should be conducted based on the identified wood and by observing the shape of the remaining components.