• Journal of Veterinary Science
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• v.22 no.6
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• pp.81.1-81.9
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• 2021

Background: Suid gammaherpesvirus 3, 4, and 5 (porcine lymphotropic herpesvirus - PLHV-1, -2, and -3) are viruses that infect domestic and feral pigs. Objectives: This study examined the presence of PLHV DNA in biological samples from free-living wild boars circulating in a Brazilian geographical region with a high density of commercial domestic pigs. Methods: Lung samples of 50 free-living wild boars were collected by exotic wildlife controller agents between 2017 and 2019 in the state of Paraná, southern Brazil. Lung and spleen fragments were obtained from six fetuses collected by hysterectomy post mortem from a pregnant sow. A polymerase chain reaction (PCR) assay using consensus primers (pan-herpesviruses) was performed to detect PLHV DNA. The samples showing positive results for PLHV DNA were submitted to single-round PCR assays with the specific primers for identifying PLHV-1 (213-S/215-As), PLHV-2 (208-S/212-As), and PLHV-3 (886s/886As). The specificity of the species-specific PCR products was assessed by nucleotide sequencing of the amplicons. Results: Forty-eight (96%) of the 50 lung samples analyzed were positive for PLHV by PCR using pan-herpesvirus primers. In 33 (68.75%) of the positive samples, at least two PLHV species were identified simultaneously. The DNA of PLHV-1, -2, and -3 was found in free-living wild boars of all ages, but not in the fetuses, even though they were from a sow that tested positive for all three viruses. Conclusion: These viruses are endemic to the population of feral pigs in the Brazilian region evaluated, as well as in domesticated pigs.

• Fisheries and Aquatic Sciences
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• v.26 no.9
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• pp.569-581
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• 2023

The morphology and genetic identification of Rasbora lateristriata and Rasbora argyrotaenia between cultivated and wild populations has never been reported. This study compares morphology and cytochrome c oxidase (COI) genes between farmed and wild stock Rasbora spp. in Java and Sumatra island, Indonesia. We analyzed the truss network measurement (TNM) characters of 80 fish using discriminant function analysis statistical tests. DNA was extracted from muscle tissue of 24 fish specimens, which was then followed by polymerase chain reaction, sequencing, phylogenetic analysis, fixation index analysis, and statistical analysis of haplotype networks. Basic Local Alignment Search Tool analysis validated the following species: R. lateristriata and R. argyrotaenia from farming (Jogjakarta); Rasbora agryotaenia (Purworejo), R. lateristriata (Purworejo and Malang), Rasbora dusonensis (Palembang), and Rasbora einthovenii (Riau) from natural resources. Based on TNM characters, Rasbora spp. were divided into four groups, referring to four distinct characters in the middle of the body. The phylogenetic tree is divided into five clades. The genetic distance between R. argyrotaenia (Jogjakarta) and R. lateristriata (Malang) populations (0.66) was significantly different (p < 0.05). R. lateristriata (Purworejo) has the highest nucleotide diversity (0.43). R. argyrotaenia from Jogjakarta and Purworejo shared the same haplotype. The pattern of gene flow among them results from the two populations' close geographic proximity and environmental effects. R. argyrotaenia had low genetic diversity, therefore, increasing heterozygosity in cultivated populations is necessary to avoid inbreeding. Otherwise, R. lateristriata (Purworejo) had a greater gene variety that could be used to develop breeding. In conclusion, the middle body parts are a distinguishing morphometric character of Rasbora spp., and the COI gene is more heterozygous in the wild population than in farmed fish, therefore, enrichment of genetic variation is required for sustainable Rasbora fish farming.

• Korean Journal of Plant Taxonomy
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• v.47 no.3
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• pp.196-198
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• 2017

Sciaphila secundiflora occurs on Jeju Island as a second species of the genus Sciaphila in Korea. This is an important finding in relation to the flora of Korea, as species of the genus Sciaphila are concentrated mainly in Indonesia and Malaysia. Few species are found on the mainland of Asia, in Japan, Taiwan, China (Hainan), tropical Africa, Australia, and on the Pacific islands. In this paper, the species S. secundiflora is described and illustrated as a new record from Korea. In addition, we suggest that the species of the genus Sciaphila should be protected with regard to its habitat characteristics and for its phytogeographic and climatic significance.

• Journal of Species Research
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• v.10 no.4
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• pp.344-349
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• 2021

The purpose of this study was to isolate and identify wild yeasts from soil of Gyeongju city, and Haemadipsa rjukjuana of Gageodo Island, characterizing unrecorded yeast strains from Korea. The molecular analysis of the D1/D2 domain of 26S rDNA of yeast was performed using the Basic Local Alignment Search Tool (BLAST). No official report exists describing these three species: one species in the genus Candida, one species in the genus Debaryomyces, and one species in the genus Solicoccozyma. Candida saitoana YL9, Debaryomyces fabryi YL1, and Solicoccozyma terrea 20g9-1 are recorded for the first time from Korea. All three strains were oval shaped and polar binding, while positive for glucose, ᴅ-xylose, and ᴅ-cellobiose. Morphological, physiological, and biochemical properties are described in the species descriptions.

• Animal Systematics, Evolution and Diversity
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• v.39 no.3
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• pp.92-98
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• 2023

Larvae of shoot flies are known to live on decaying plants or to infest the stems of wild and crop plants. Among them, some species are economically very important pests that damage fruits or cereal crops in Old world tropics and subtropics. In Korea, three species, Atherigona orientalis, A. oryzae, and A. soccata, are managed as quarantine pests. To date, a total of five species, A. (Acritochaeta) orientalis Schiner, A. (Atherigona) bifurca Suh and Kwon, A. (A.) biseta Karl, A. (A.) falcata (Thomson) and A. (A.) oryzae Malloch, including two quarantine pests, have been recorded in Korean fauna. During the survey of Korean houseflies, the authors discovered two new unrecorded species, A. (A.) miliaceae Malloch and A. (A.) reversura Villeneuve. The diagnoses and illustrations of these species are provided in addition to the key to the Korean Atherigona species.

• The Korean Journal of Mycology
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• v.48 no.3
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• pp.237-249
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• 2020

The goal of this study was to isolate wild yeasts from waters and soils in Sangjubo of Nakdong river and Daechung dam in Daejeon city, Korea and investigate characteristics of previously unrecorded novel wild yeasts. In total, 94 strains from 39 different species of wild yeasts were isolated from 47 soils and waters samples obtained from Sangjubo in Nakdong river. Among these, 3 strains, including Rhodosporidium azoricum JSL-GRU-009, represented novel yeast strains which were previous recorded. Two hundred nine strains from 105 different species of wild yeasts were isolated from 130 soils and water samples obtained from Daechungdaegyo and Daechung dam of Shintanjin in Daejeon city, Korea. Ten strains including Bullera pseudoalba JSL-GRU-005 also represented newly recorded strains in Korea. All the 13 previously unrecorded yeasts were oval or global in shape, and nine strains, including R. azoricum JSL-GRU-009, formed ascospores. Ten strains grew well in vitamin-free medium. R. azoricum JSL-GRU-009 grew well in 20% glucose-containing yeast extract-peptone-dextrose (YPD) medium and 3 strains, including Rhodotorula oryzicola JSL-GRU-003, grew well in 5% NaCl-containing YPD medium.

• Journal of Nutrition and Health
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• v.44 no.1
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• pp.16-28
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• 2011

Oxidative stress leads to the induction of cellular oxidative damage, which may cause adverse modifications of DNA, proteins, and lipids. The production of reactive species during oxidative stress contributes to the pathogenesis of many diseases. Antioxidant defenses can neutralize reactive oxygen species and protect against oxidative damage. The aim of this study was to assess the antioxidant status and the degree of DNA damage in Korean young adults using glutathione s-transferase (GST) polymorphisms. The GSTM1 and GSTT1 genotypes were characterized in 245 healthy young adults by smoking status, and their oxidative DNA damage in lymphocytes and antioxidant status were assessed by GST genotype. General characteristics were investigated by simple questionnaire. From the blood of the subjects, GST genotypes; degree of DNA damage in lymphocytes; the erythrocyte activities of superoxide dismutase, catalase, and glutathione peroxidase; plasma concentrations of total peroxyl radical-trapping potential (TRAP), vitamin C, ${\alpha}$- and ${\gamma}$-tocopherol, ${\alpha}$- and ${\beta}$-carotene and cryptoxanthin, as well as plasma lipid profiles, conjugated diene (CD), GOT, and GPT were analyzed. Of the 245 subjects studied, 23.2% were GSTM1 wild genotypes and 33.4% were GSTT1 wild genotype. No difference in erythrocyte activities of superoxide dismutase, catalase, or glutathione peroxidase, and the plasma TRAP level, CD, GOT, and GPT levels were observed between smokers and non-smokers categorized by GSTM1 or GSTT1 genotype. Plasma levels of ${\alpha}$- and ${\gamma}$-tocopherol increased significantly in smokers with the GSTT1 wild genotype (p < 0.05); however, plasma level of ${\alpha}$-carotene decreased significantly in non-smokers with the GSTM1 wild genotype (p < 0.05). DNA damage assessed by the Comet assay was significantly higher in non-smokers with the GSTM1 genotype; whereas DNA damage was significantly lower in non-smokers with the GSTT1 genotype. Total cholesterol and LDL cholesterol levels were significantly higher in non-smokers with the GSTT1 genotype than those with the GSTT1 wild genotype (p < 0.05). In conclusion, the GSTM1 genotype or the GSTT1 wild genotype in non-smokers aggravated their antioxidant status through DNA damage of lymphocytes; however, the GSTT1 wild type in non-smokers had normal plasma total cholesterol and LDL-cholesterol levels. This finding confirms that GST polymorphisms could be an important determinant of antioxidant status and plasma lipid profiles in non-smoking young adults. Further study is necessary to clarify the antioxidant status and/or lipid profiles of smokers with the GST polymorphism and to conduct a study with significantly more subjects.

• Korean Journal of Plant Resources
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• v.2 no.2
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• pp.259-278
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• 1989

The plants medicinal resources of Mt. Eb-aon were investifeted 18times from JuLy, 1988 to August, 1989. In orther to analyze the vegeta-tion of Eb-am mountain area, medical wild plants structure and distr-ibution. Medical wild Plants of Eb-am mountain consisted of 100 familis, 337 species in alL. The resources of important herb drugs were, Graminea, Liliaceae, Molaceae, Polygonaceae, Ranunculaceae, Brassicaceae, Rosaceae, Drupaceae, Fabaecae, Apiaceae, Labiatae, Solanaceae, Scrophulariaceae, Rubiaceae, CampanuLaceae, ComposLtae, The herb drufs were Comparatively more than in other mountains in our country.

• The Korean Journal of Mycology
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• v.43 no.1
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• pp.64-67
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• 2015

Several kinds of wild yeasts were isolated and identified from some cereals. A total of twenty six yeast strains were isolated from eleven kinds of cereals. Among twenty six yeast strains, Saccharomyces cerevisiae were five strains and Pseudozyma antarctica were four strains. Five species of Cryptococcus including Cryptococcus magnus were also isolated. Pseudizyma aphidis were isolated from black bean, and Saccharomyces cerevisiae, Cryptococcus flavescens, Cryptococcus magnus and Hannaella zeae were also isolated from glutinous millet.

• The Korean Journal of Soil Zoology
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• v.3 no.2
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• pp.72-77
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• 1998

This study was carried out to investigate the composition of soil microarthropods in pasture at the Unbong Area, to fulfil this purpose, samples were taken from National livestock research institute Namwon branch from 24. June, 1998 to 18. Setember. The results of this study were as follow. Collembola was hight in cattle and goat pasture, and Acarina was hight in wild and gathering pasture. Oribated mites were also hight in wild and gathering pasture more than cattle and goat pasture. Oribatid mites as a vectors of cestodes were identified 7 species of Carabodes peniculatus, Carabodes sp., Scheloribates laevigatus, Scheloribates latipes, Scheloribates rigidisetosus, Galumna cuneata and Galumna sp. The diversity indices showed hight in wild and gathering pasture. According to the MGP analysis I was type GP, and analysis II was type P.