• Title/Summary/Keyword: wild plant

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Habitat preference of wild boar (Sus scrofa) for feeding in cool-temperate forests

  • Kim, Youngjin;Cho, Soyeon;Choung, Yeonsook
    • Journal of Ecology and Environment
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    • v.43 no.3
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    • pp.297-304
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    • 2019
  • Background: The growing wild boar population has become a social issue and its feeding characteristics could affect the physical condition and the plant species composition in the South Korean forests. We aimed to reveal the preference of the wild boar on forest type and site condition as feeding grounds in two cool-temperate forested national parks, Odaesan and Seoraksan, in order to provide information to manage the growing population. Results: The 75 plots (53.6%) out of 140 plots were used as feeding grounds by the wild boar, implying a considerably large population. Especially, the observation frequency as feeding ground was the highest in Quercus forests (73.3%), and it was significantly more preferred than deciduous forest type (44.2%) and coniferous forest type (32.4%) (${\chi}^2=17.591$, p < 0.001). Significantly more and deeper pits were found in Quercus forests. Moreover, high elevation and gentle slope ridge were relatively preferred regardless of forest distribution. Conclusions: South Korean forests are growing qualitatively and quantitatively. Particularly, Quercus forest area has increased markedly, while coniferous forest area has decreased. Since the Quercus forest provides rich food sources for the wild boar, the enlargement of this forest type is expected to increase the wild boar population. The forests located at high elevations have high species diversity, and it is expected that these forests will be greatly affected by the increase in the wild boar population as preferred feeding grounds.

A Basic Study on Development of Medical Wild Plant Resouces in Mt. CHU-WOL (추월산 한약자원식물의 분포 및 분류체계에 관한 기초적 연구)

  • EuiSooYoon
    • Korean Journal of Plant Resources
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    • v.3 no.1
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    • pp.41-63
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    • 1990
  • The plants medicinal resources of Mt. Chu-woL were investigated 21times from APril, 1975 to August , 1977 and from JuLy, 1988 to August 1989In orther to analyze the vegetation of Chu-woL mountain area , medical wildplants structure and distribution. MedicaL wild plants of Chu-woL moun-tain consisted of 113 familis, 428 species in alL. The resources of important herb drugs were Polypodiaceae, Graminea, Liliaceae , Polygonaceae,Ranunculaceae, Brassicaceae, Rosaceae , Fabaceae, Apiaceae, tabiatae, so-lanaceae, Scrophulariaceae, Campanulaceae, Compositae. The herb drugswere comparatively more than in other mountains in our country .

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Cloning, Characterization, and Functional Analysis of Maize DEHYDRIN2

  • Paek, Nam-Chon;Jung, Hun-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.2
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    • pp.116-122
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    • 2002
  • Dehydrins (LEA Dll proteins) are one of the typical families of plant proteins that accumulate in response to dehydration, cold stress, abscisic acid, or during seed maturation. A 1.3-kb cDNA was cloned from a cDNA expression library of 5-day-old germinating maize scutellums under drought stress. The deduced protein sequence indicated a dehydrin gene encoding SK$_3$ LEA protein typically expressed during cold acclimation, but not by drought stress in barley and wheat. Thus, it was named maize DEHYDRIN2 (ZmDhn2). It accumulates rapidly and highly in drought-stressed scutellum and leaf tissues at any stage, but not under cold stress. ZmDhn2 gene was transformed into Arabidopsis thaliana for functional analysis under drought condition. From electrolyte leakage test, no significant difference showed between wild type and transformants under normal growth condition, but the leakage level of electrolyte in wild type plants was about 3 times as high as that in the transformed plants under drought stress. It suggests that ZmDHN2 playa role in increasing drought tolerance.

The Resource Plants in Construct-Reserved Site of Ecological Forest, Chungcheongbuk-do, Korea (충청북도 생태숲 조성예정지의 자원식물상)

  • You Ju-Han;Cho Heung-Won;Jin Yeon-Hee;Yun Hee-Bin;Lee Gwi-Yong;Han Ju-Hwan;Lee Cheol-Hee
    • Korean Journal of Plant Resources
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    • v.18 no.3
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    • pp.390-402
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    • 2005
  • This study was carried out systematically to grasp the resource plants in construct-reserved site of ecological forest, Jecheon-si, Chungcheongbuk-do, Korea. This site was located at $37^{\circ}12'$ in latitude and $127^{\circ}57'$ in longitude and the period of survey was from March to October, 2004. The vascular plants were summarized as 371 taxa; 83 families, 238 genera, 324 species, 44 varieties and 3 forma. And in the results of survey on resource plants, we confirmed 178 taxa of ornamental plants$(47.98\%)$, 232 taxa of edible plants$(62.53\%)$, 257 taxa of medicinal plants$(69.27\%)$ and 154 taxa of other useful plants$(41.51\%)$. The rare and endangered plants designated by Korea Forest Service were 4 taxa; Eranthis stellata, Paeonia japonica, Viola albida and Scopolia japonica.

In vitro induction of hairy root from isoflavones-producing Korean wild arrowroot Pueraria lobata

  • Kim, Soo-Jung;Cha, Min-Seok;Lee, Eun-Ji;Kim, In-Hye;Kwon, Jung-Eun;Kang, Se-Chan;Park, Tae-Ho
    • Journal of Plant Biotechnology
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    • v.39 no.3
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    • pp.205-211
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    • 2012
  • Pueraria lobata is a perennial legume plant, widely distributed in the countries of East Asia. It is a medicinally important leguminous plant and produces various isoflavones such as puerarin, daidzein etc which have potential for preventing several chronic diseases including osteoporosis, cardiovascular disease and cancer. In this study, we tried to induce hairy roots in vitro from Korean wild arrowroot P. lobata and investigated the effects of hormones and light conditions. Initially leaf and stem segments were infected with Agrobacterium rhizogenes and incubated in different conditions. Hairy roots were induced from only stem segments and the induction was best at dark condition and the presence of IBA during incubation. Secondary roots were also significantly much more induced at the dark condition than at the 16 hours light condition. Among plant growth regulators of auxin, IBA was best for secondary root formation while 2,4-D, IAA and NAA produced callus or less hairy roots. The presence of the foreign gene rolC transferred by A. rhizogenes that plays a major role in hairy root induction was confirmed by PCR. The accumulation of isoflavones such as puerarin and daidzin was also confirmed. These results will facilitate mass production of hairy root and can be used for the production of functional substances from wild arrowroots.

Constitutive Expression of Small Heat Shock Protein Increases Thermotolerance in Transgenic Plant (저 분자량 Heat Shock Protein의 항상적 발현에 의한 형질전판 식물체의 고온내성 증가)

  • 이병현
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.1
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    • pp.13-18
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    • 2000
  • To investigate the function of chloroplast small HSP, transgenic tobacco plants (Nicotiana tabacum L. cv. Samsun) that constitutively overexpress the chloroplast small HSP (NtHSP21) from N. tabacum cv. Petit Havana SR1 were generated. Five homozygous lines of transformants showing different constitutive expression levels of the NtHSP21 were selected. To determine whether constitutive overexpression of NtHSP21 protein affects thermotolerance, wild-type and transformants were grown in Petri dishes, heat-stressed at 52$^{\circ}C$ for 45 min, and then incubated in normal growth condition. When heat-stressed wild-type plantlets were incubated at $25^{\circ}C$, leaf color gradually became white and all trio plantlets finally died within a week. As for the transformants, however, more than 70% of them remained green and survived under the conditions in which all the wild-type plants were dying. It was also found that the levels of NtHSP21 were correlated with the degree of thermotolerance. These results suggest that the NtHSP21 protein in transformants is responsible for the increase in thermotolerance.

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Total Phenolics Levels and Antioxidant Properties in Methanol Extracts from Several Vietnamese Wild Plants

  • Boo, Hee-Ock;Kim, Tae-Soon;Koshio, Kaihei;Shin, Jeoung-Hwa;Chon, Sang-Uk
    • Korean Journal of Plant Resources
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    • v.24 no.6
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    • pp.659-665
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    • 2011
  • The aim of this study was to determine total phenolics (TP) content, total flavonoids (TF) level, and antioxidant activity of methanol extracts from leaf parts of 15 Vietnamese wild plants. TP content showed the highest amount in methanol extracts from Altenanthera sessilis ($409.7{\pm}1.4$ ppm), and followed by Eclipta prostrata ($183.6{\pm}3.8$ ppm) and Cassia tora ($159.3{\pm}5.7$ ppm). The highest TF level also was found in A. sessilis ($156.3{\pm}1.7$ ppm), followed by E. prostrata, and C. tora, showing similar tendency to TP. Methanol extracts of A. sessilis, E. prostrate and C. tora at 1000 ppm exhibited the highest DPPH radical scavenging activity by 94.5, 92.6 and 92.7%, respectively. The methanol extracts from A. sessilis showed the highest ABTS and nitrite scavenging activities by 97% and 92%, respectively. The highest correlation coefficient ($r^2$) was 0.9522 between TP and TF, and followed by 0.8919 between DPPH and ABTS activities. However, polyphenols and antioxidant activities showed low correlation coefficients, ranging from $r^2=0.4114$ to 0.4826. It was concluded that Vietnamese wild plants contain polyphenol compounds with antioxidant activities depending on plant species.

High-Throughput Development of Polymorphic Simple Sequence Repeat Markers Using Two Whole Genome Sequence Data in Peucedanum japonicum

  • Lee, Junki;Joh, Ho Jun;Kim, Nam-Hoon;Lee, Sang-Choon;Jang, Woojong;Choi, Beom Soon;Yu, Yeisoo;Yang, Tae-Jin
    • Plant Breeding and Biotechnology
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    • v.5 no.2
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    • pp.134-142
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    • 2017
  • Resource plants are important and have strong potential for a variety of utilities as crops or pharmaceutical materials. However, most resource plants remain wild and thus their utility for breeding and biotechnology is limited. Molecular markers are useful to initiate genetic study and molecular breeding for these understudied resource plants. We collected various wild collections of Peucedanum japonicum which is indigenous resource plants utilized as oriental medicine and leafy vegetables in Korea. In this study, we produced two independent whole genome sequences (WGSs) from two collections and identified large scale polymorphic simple sequence repeat (pSSR) based on our pipeline to develop SSR markers based on comparison of two WGSs. We identified a total of 452 candidate pSSR contigs. To confirm the accuracy and utility of pSSR, we designed ten SSR primer pairs and successfully applied those to seven collections of P. japonicum. The WGS and pSSR candidates identified in this study will be useful resource for genetic research and breeding purpose for the valuable resource plant, P. japonicum.

Growth, Nitrogen Metabolism, and Nodulation of Hypernodulating Soybean Mutant Affected by Soil Fertility

  • Ha, Bo-Keun;Lee, Suk-Ha
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.2
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    • pp.145-149
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    • 2001
  • This study was performed to evaluate the growth and nodulation characters of hypernodulating soy-bean mutant, SS2-2, and to know the growth and yield performance of the mutant in infertile soil. Soil fertility was adjusted by mixing the different ratios of soil components including clay, river sand, and horticultural bed, which resulted in fertile and infertile soil. Dry weight, nitrogen concentration, and leaf nitrate reductase of each plant were measured around V6 stage (47 days after planting) and around R3 stage (82 days after planting). There were significant effects of soil fertility and soybean genotype on the total dry weights including root, nodule, stem, leaf, and pod dry weight at V6 and R3 stages. Total dry weight of hypernodulating mutant, SS2-2, was clearly less than that of its wild type, Sinpaldalkong 2. However, nodule development on the roots of SS2-2 was much greater than that of Sinpaldalkong 2, regardless of soil fertility. Though SS2-2 was smaller in plant size than Sinpaldalkong 2, genotypic difference in total nitrogen content was not significant at both V6 and R3 stages because SS2-2 fixed more nitrogen biologically than its wild type in the root nodule. The SS2-2 mutant showed lower plant yield in both infertile and fertile soil. The SS2-2 contained more crude seed protein than Sinpaldalkong 2, and was characterized with reduced top and root growth.

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The Endo-β-1,4-Glucanase of Bacillus amyloliquefaciens Is Required for Optimum Endophytic Colonization of Plants

  • Fan, Xiaojing;Yang, Ruixian;Qiu, Sixin;Cai, Xueqing;Zou, Huasong;Hu, Fangping
    • Journal of Microbiology and Biotechnology
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    • v.26 no.5
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    • pp.946-952
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    • 2016
  • The eglS gene in Bacillus amyloliquefaciens encodes an endo-β-1,4-glucanase that belongs to glycosyl hydrolase family 5. In this study, a disruption mutant of gene eglS was constructed to examine its role in bacterial adaptation in plants. The mutant TB2k, eglS gene inactivated bacterial strain, was remarkably impaired in extracellular cellulase activity. When inoculated on Brassica campestris, the TB2k population was reduced by more than 60% compared with the wild-type strain in the root, stem, and leaf tissues. Overexpression of eglS in the wild-type strain increased the bacteria population in the plant tissues. Further studies revealed that the transcription level of eglS was correlated with bacterial population. These data demonstrate that endo-β-1,4-glucanase of B. amyloliquefaciens is required for its optimal endophytic colonization.