• Korean Journal of Poultry Science
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• v.25 no.3
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• pp.113-118
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• 1998

These experiments were conducted to develope the artificial insemination methods of wild duct (Anas platyhychis platylyachos) . The characteristics of erect phallus and semen collected by the abdominal massage method were investigated in wild duck. The erection and withdrawal time of phallus were 50.70${\pm}$18.66 and 92.58${\pm}$51.95 sec. , respectively, in wild duck. The length, long and short diameter of erect phallus were 3.98${\pm}$0.49crn, 1.53${\pm}$0.15cm and 1.05${\pm}$0.04 cm, respectively, and the spiral grooves of erect phallus were 4 in wild duck. The volume of semen, concentration of spermatozoa and total sperm of an ejaculate were 0.18${\pm}$0.06 ml, 2.84${\pm}$0.03 x 10 9 /ml and 0.52${\pm}$0.21 x 10 cells, respectively, in wild duck. The motility of sperm and pH of semen were 49.06${\pm}$14. 35 and 7.6${\pm}$0.14 in wild duck.

• Parasites, Hosts and Diseases
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• v.56 no.4
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• pp.375-378
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• 2018

A total of 44 adult or juvenile nutrias were necropsied for disease survey. A large nodule was found in the liver of a nutria. The histopathological specimen of the hepatic nodule was microscopically examined, and sectional worms were found in the bile duct. The worms showed a tegument with spines, highly branches of vitelline glands and intestine. Finally, we histopathologically confirmed fascioliasis in a wild nutria. In the present study, a case of fascioliasis in a wild nutria is first confirmed in Korea.

• Journal of Veterinary Clinics
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• v.31 no.6
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• pp.531-534
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• 2014

A 3-year-old intact mixed hunting dog was referred to Gyeongsang Animal Medical Center with the clinical signs of vomiting, dyspnea, anorexia. The dogs had traumatic history that wounded at abdominal wall during the wild boar hunting 2 week ago. The dogs was diagnosed as extrahepatic biliary tract obstruction (EHBO) secondary to traumatic hernia. Diagnosis was based on radiographic features of a diaphragmatic hernia on survey radiography, dilated bile duct in abdominal ultrasonography and complete recovery due to the return of displaced organs into the peritoneal cavity and repair of the diaphragmatic rent. Diagnostic imaging and surgical intervention may result in a favorable outcome in dog with post hepatic obstruction secondary to diaphragmatic hernia. This case emphasizes the strangulation of bile duct by diaphragmatic rupture could be the cause of EHBO.

• Journal of Veterinary Science
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• v.21 no.2
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• pp.26.1-26.14
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• 2020

Pancreatic ductal adenocarcinoma is a lethal cancer type that is associated with multiple gene mutations in somatic cells. Genetically engineered mouse is hardly applicable for developing a pancreatic cancer model, and the xenograft model poses a limitation in the reflection of early stage pancreatic cancer. Thus, in vivo somatic cell gene engineering with clustered regularly interspaced short palindromic repeats is drawing increasing attention for generating an animal model of pancreatic cancer. In this study, we selected Kras, Trp53, Ink4a, Smad4, and Brca2 as target genes, and applied Campylobacter jejuni Cas9 (CjCas9) and Streptococcus pyogens Cas9 (SpCas9) for developing pancreatic cancer using adeno associated virus (AAV) transduction. After confirming multifocal and diffuse transduction of AAV2, we generated SpCas9 overexpression mice, which exhibited high double-strand DNA breakage (DSB) in target genes and pancreatic intraepithelial neoplasia (PanIN) lesions with two AAV transductions; however, wild-type (WT) mice with three AAV transductions did not develop PanIN. Furthermore, small-sized Cjcas9 was applied to WT mice with two AAV system, which, in addition, developed high extensive DSB and PanIN lesions. Histological changes and expression of cancer markers such as Ki67, cytokeratin, Mucin5a, alpha smooth muscle actin in duct and islet cells were observed. In addition, the study revealed several findings such as 1) multiple DSB potential of AAV-CjCas9, 2) peri-ductal lymphocyte infiltration, 3) multi-focal cancer marker expression, and 4) requirement of > 12 months for initiation of PanIN in AAV mediated targeting. In this study, we present a useful tool for in vivo cancer modeling that would be applicable for other disease models as well.

• Journal of Environmental Impact Assessment
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• v.7 no.2
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• pp.15-26
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• 1998

In order to assess the present status of the fragmentation of ecosystem and the building of ecological corridor, 79 EISs(Environment Impact Statements) which were conducted in Kyunggido and Kangwondo have been reviewed using 36 analysing factor. The results obtained from this study were as follows: a. The most frequently appearing type of ecosystem fragmentation was 'mountain vs. mountain type in topography' and '2 vs. 2 grades in the degree of green naturality.' b. The most frequently listed fauna in EISs included squirrels and wild rabbits for mammals, grass snake and pit viper for reptiles, and tree frog and true tree frog for amphibians. c. Among the EISs for 50 projects, the construction of ecological corridor mentioned in only 4 projects and other structures such as drainage duct and closed conduit in 14 projects were suggested as ecological corridor. There were no corridors suggested in 32 projects. Thus, in case of using other structures for wildlife animals but ecological corridors, it should be accompanied by incidental facilities for including animal movement.

• Parasites, Hosts and Diseases
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• v.57 no.3
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• pp.299-302
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• 2019

A clonorchiasis case in a captive leopard cat, Prionailurus bengalensis euptilurus, was confirmed by ultrasonographic findings and egg morphologies found in the bile juice sample in the Korea. The leopard cat was introduced from the wild habitat of Gyeongsangnam-do, to Cheongju Zoo in Cheongju-si, Chungcheongbuk-do, Korea in August 2014. Physical examinations were basically performed for quarantine and check-up health. The cat was comparatively good in health except anorexia. The cyst-like bile duct dilation and the increased echogenicity of gall bladder wall and hepatic parenchyma were observed by ultrasonography. Ultrasound-guided needle biopsy was conducted for collecting bile juice and the specimens were observed under light microscope. The numerous small trematode eggs were detected in the bile juice sample of the light microscopy. The eggs were $25-33(28{\pm}3){\mu}m$ by $18-22(20{\pm}1){\mu}m$ in size and showed typical characteristics of Clonorchis sinensis egg, i.e., a dominantly developed operculum, shoulder rim and dust-like wrinkles in surface. To treat the liver fluke infection, 20 mg/kg of praziquantel was orally administered only once to the case. Follow-up studies including fecal examinations were conducted during 2 years after treatment. But no more eggs were detected from the case. In the present study, we described the first clonorchiasis case of leopard cat, which was confirmed by ultrasonographic findings and egg morphologies from the bile juice sample in Korea.

• Journal of Veterinary Clinics
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• v.26 no.4
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• pp.379-385
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• 2009

Tetragomphius procyonis Baylis & Daubney, 1923 were obtained from the pancreatic duct of a naturally infected Eurasian badger, Meles meles, which was submitted to animal hospital for parasitic diagnosis from Gyeryongsan National Park in Korea. The hookworms were examined by light and scanning electron microscope. The length of body measured male 15.0-18.8 mm, female 21.5-25.5 mm, respectively. In both sex, the ventral cutting plates of oral margin are much reduced and elongated latero-dorsally, the dorsal cutting plate is located long follow doral margin of the oral opening. The buccal capsule is cup-shaped and thicken with four cusped tooth at its base. The copulatory bursa has elongated ventral lobes and their large rays are parallel, while the dorsal lobe with its supporting rays is slightly split in two. The slender spicules are filariform and very long (8.7-9.3 mm), and their tips are fused together. The hookworm has following characters: dorsal cone on the both sex, gubernaculum on the male and terminal spine on the female tail absent; vulva is opening in the juction of the fourths and fifths of the body; dorsal ray with two long stems. The eggs from the uterus are 16 cell stages and those from the rectal feces, 32 cell stages. The size of eggs is 77.48-83.45 ${\times}$ 50.75-63.38 ${\mu}m$.

• Korean Journal of Ichthyology
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• v.20 no.4
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• pp.255-262
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• 2008

The endemic Korean stumpy bullhead Pseudobagrus brevicorpus is a first-grade endangered wild fish as designated by the Ministry of Environment of Korea. As part of its restoration and proliferation effort, a histological study of this fish was carried out to investigate sex differentiation and gonadal development based on F1 generation individuals obtained by artificial breeding. On days 4~5 after hatching, a pair of genital ridges including clusters of primordial germ cells was observed between the gut and the mesonephric duct. On days 20 after hatching, the ovary began to initially differentiate and contained early oocytes with chromatin-nucleolus and peri-nucleolus stages on days 30~40 after hatching. As yolk material accumulated after day 80 from hatching, the oocytes grew increasingly large and were surrounded by a distinct follicular layer. On days 306 after hatching, the oocytes grew toward a mature ovum. In the males, the testis was distinguished by emergence of spermatogonium cells on 25 days after hatching, and day 40 after hatching it contained a small number of seminal lobes forming cysts. From 173 days after hatching, the testis consisted of numerous enlarged seminal lobes including spermatocytes and spermatids. Over 14 months after hatching, some seminal lumens were filled with spermatozoa.

• Applied Microscopy
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• v.41 no.3
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• pp.205-213
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• 2011

Numerous anisakids were parasitic near the digestive tract of the black rockfish, Sebastes schlegeli and some anisakids observed on the liver of the host. Anisakids in the host were identified three species (Hysterothylacium sp., Anisakis simplex, A. pegreffii) and Hysterothylacium sp. was the high occurrence of anisakid worms in the host. Hysterothylacium sp. was shorter and thinner than A. simplex. Both of anisakids observed lip, mouth, nerve ring, excretory pore and excretory duct in the anterior portion. In the mid portion, anisakids had esophagus, ventriculus and intestine and especially, Hysterothylacium sp. had intestinal ceacum and ventricular appendage. There was conical nodulose apex at the end of the posterior portion in Hysterothylacium sp. and spine in A. simplex. SEM examination revealed that there was three lip near the mouth of Hysterothylacium sp. Dorsal lip was approximately 65 ${\mu}m$ and paried lateroventral lip were approximately 60 ${\mu}m$ in the width. All lips were found double papilla (approximately 8 ${\mu}m$ in the width). The body width of the Hysterothylacium sp. and A. simplex was approximately 480 ${\mu}m$ and 900 ${\mu}m$ respectively. The hight of the lateral alae was about 7 ${\mu}m$ and width of papilla on the cornical nodulose apex was about 3.3 ${\mu}m$ in Hysterothylacium sp. The hight of spine was approximately 20 ${\mu}m$ in A. simplex. There was mainly Hysterothylacium sp. in the intestinal lumen of the host. The nematod worms were parasitic near the mucosal fold and in the submucosal. In the mucosal epidermal layer, it was increased mucous cells by the infection of the parasites.