• Journal of Biomedical Engineering Research
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• v.37 no.5
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• pp.168-177
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• 2016

In this paper we present an implantable pressure sensor to measure real-time blood pressure by monitoring mechanical movement of artery. Sensor is composed of inductors (L) and capacitors (C) which are formed by microfabrication and direct bonding on two biocompatible substrates (quartz). When electrical potential is applied to the sensor, the inductors and capacitors generates a LC resonance circuit and produce characteristic resonant frequencies. Real-time variation of the resonant frequency is monitored by an external measurement system using inductive coupling. Structural and electrical simulation was performed by Computer Aided Engineering (CAE) programs, ANSYS and HFSS, to optimize geometry of sensor. Ultrafast laser (femto-second) cutting and MEMS process were executed as sensor fabrication methods with consideration of brittleness of the substrate and small radial artery size. After whole fabrication processes, we got sensors of $3mm{\times}15mm{\times}0.5mm$. Resonant frequency of the sensor was around 90 MHz at atmosphere (760 mmHg), and the sensor has good linearity without any hysteresis. Longterm (5 years) stability of the sensor was verified by thermal acceleration testing with Arrhenius model. Moreover, in-vitro cytotoxicity test was done to show biocompatiblity of the sensor and validation of real-time blood pressure measurement was verified with animal test by implant of the sensor. By integration with development of external interrogation system, the proposed sensor system will be a promising method to measure real-time blood pressure.

• Analytical Science and Technology
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• v.5 no.4
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• pp.349-358
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• 1992

The direct determination of lead in the whole-blood by graphite furnace atomic absorption spectrometric analysis was carried out by using the sample which was diluted five-fold with 1% Triton X-100. Matrix modification was tried to remove the interferences of blood matrix and also to get the optimum analytical condition. Good agreement with certificated values in reference materials(bovine blood) supplied by comparison program in Japan was obstained when 1% $(NH_4)_2HPO_4$ as matrix modifier and ashing temperature, $700^{\circ}C$ were used or 1% $(NH_4)_2HPO_4$ and 0.1% $PdCl_2$ as matrix modifier and ashed at $700^{\circ}C$. Standard deviations were appeared as 2.2~6.3% for 1% $(NH_4)_2HPO_4$ and 3.1~9.1% for 1% $(NH_4)_2HPO_4$ and 0.1% $PdCl_2$ in the range of $31{\sim}624{\mu}gPb/l$.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.1
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• pp.80-85
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• 2018

Objective: This study was conducted to investigate the effect of corn grain particle size on ruminant fermentation and blood metabolites in Holstein steers fed total mixed ration (TMR) as a basal diet to explain fundamental data of corn grain for cattle in Korea. Methods: Four ruminally cannulated Holstein steers (body weight $592{\pm}29.9kg$) fed TMR as a basal diet were housed individually in an auto temperature and humidity modulated chamber ($24^{\circ}C$ and 60% for 22 h/d). Treatments in a $4{\times}4$ Latin square design were TMR only (control), TMR with whole corn grain (WC), coarsely ground corn grain (CC), and finely ground corn grain (FC), respectively. The corn feeds substituted for 20% energy intake of TMR intake. To measure the ruminal pH, ammonia N, and volatile fatty acids (VFA), ruminal digesta was sampled through ruminal cannula at 1 h intervals after the morning feeding to determine ruminal fermentation characteristics. Blood was sampled via the jugular vein after the ruminal digesta sampling. Results: There was no difference in dry matter (DM) intake between different corn particle size because the DM intake was restricted to 1.66% of body weight. Different corn particle size did not change mean ammonia N and total VFA concentrations whereas lower (p<0.05) ruminal pH and a ratio of acetate to propionate, and higher (p<0.05) propionate concentration were noted when the steers consumed CC compared with WC and FC. Concentration of blood metabolites were not affected by different particle size of corn grain except for blood triglyceride concentration, which was significantly (p<0.05) increased by FC. Conclusion: Results indicate that feeding CC may increase feed digestion in the rumen, whereas the FC group seemed to obtain inadequate corn retention time for microbial degradation in the rumen.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.1
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• pp.1-9
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• 2017

This research was conducted to investigate the effects of feeding high and low forage diets with different forage sources on rumen fermentation characteristics and blood parameters of Holstein cows during the dry period. Eight Holstein cows were completely randomized assigned to two groups and repeated measurement was utilized in the analysis. Cows in two treatments were fed with diets with high (F:C = 70:30, 70F; forage source: mixed-sowing whole crop barley and Italian ryegrass silage, BIRG) and low (F:C = 55:45, 55F; forage source: tall fescue hay, TF) forage level. Rumen fluid pH was higher in 70F group. Levels of acetic acid, propionic acid, and butyric acid showed a similar pattern: from the lowest value at 07:30 h to the highest at 10:30 h and then decreased in both groups. The ratio of acetic acid to propionic acid was significantly higher (p < 0.05) in 55F group at 09:30 and 10:30 h. Rumen fluid $NH_3-N$ concentrations were significantly higher (p < 0.05) in 70F group at 09:30 and 10:30 h. Blood urea nitrogen was significantly higher (p < 0.05) in 70F group. It was concluded that BIRG based diet with a high forage level had no adverse effects on rumen fermentation, some blood chemical parameters, and immune system in dry Holstein cows and could be used as a forage source instead of imported TF.

• Korean Journal of Community Nutrition
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• v.5 no.2
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• pp.172-184
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• 2000

To establish a basic cohort which can survey the nutrient and food intake related to the health status of the middle and old-aged vertically, a longitudinal survey of 110 adults over forty years old was conducted three times in Chon-ju city over 5 years (1994-1999), and the influences of health status by changes in their nutrient and food intake were examined in this study. The results were as follows ; The number of subjects was 110(43 man, 67 women), 10.0% in their forties, 28.2% in their fifties, 45.4% in their sixties 6.4% over seventy, and their average age was 61.4. There were decrease of height about 1cm , increase of body fat % and few changes of BMI during the testing period. As the subjects were aging, the percentage of anemia increased, but averages of blood pressure and blood glucose(men : 91.8mg/㎗, women : 90.3mg/㎗) were constant. Total cholesterol increased from 196.7mg/㎗ to 212.6mg/㎗(P 0.05) over five years, and the numerical values for women were higher than that of men. In many cases, lack of nutrient intake was shown, as compared with the Recommended Dietary allowance(RDA) especially calcium and vitamin A(71.7%, 64.9% of RDA). Grains intake showed a tendency to decrease(P 0.01). On the contrary, fruit intake showed a tendency to increase(P 0.001). On whole meal, plant foods were 85.1% and animal foods were 14.9%. As dietary factors influencing health status were investigated, blood glucose was influenced by vitamin B$_2$, potatoes and triglyceride, DBP and SBP were affected by vitamin B$_1$, vitamin C, legumes, fishes, triglyceride and age. Total cholesterol was under the influence of triglyceride, milk, seeds and fat. BMI was influenced by iron, SBP, animal food, and age.

• Journal of English Language & Literature
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• v.57 no.1
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• pp.147-170
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• 2011

This paper elucidates the characteristics of transnational adoption, estimates the possibility of beyond-borders identity of transnational adoptees, and tries to analyze Jane Jeong Trenka's The Language of Blood in its context. Though it has been regarded as one of the most humanitarian ways of helping orphans and poor children of the world, transnational adoption, a one-way flow of children from poor Asian countries to rich white countries, has been operated under the market logic between countries. Transnational adoptees, who had been abandoned and forced to be taken away from their birth mother, and later, to fulfill the desire of white parents for a perfect family, perform an ideological labor, serving to make the heterogeneous nuclear family complete. Korean transnational adoptees, forced to transcend the borders of nation, culture, and ethnicity, experience racial conflict and alienation in white adoptive family and society. Their diaspora experience of violent dislocation creates frustration and confusion in establishing their identity as a whole being. When they return to Korea to find their birth mother and their true identity, Korean adoptees, however, are faced with other obstructing issues, such as language problem, culture conflict, and maternal nationalism. Finally, Korean transnational adoptees reject Korean nationalism discourse based on blood, and try to redefine themselves as beyond-borders subjectivities with new and fluid identities. Jane Jeong Trenka's The Language of Blood, an autobiographical novel based on her experiences as a transnational adoptee, represents a Korean adopted girl's personal, cultural, and racial conflict within her white adoptive family, and questions the image of benevolent white mother and the myth of multiculturalism. The novel further represents Jane's return to Korea to find out her true identity, and shows Jane's disappointment and alienation in her birth country due to her ignorance of language and culture. Returning to USA again, and trying to be reconciled with her American mother, Jane shows the promise of accepting her new identity capable of transcending the borders, and thus, the possibility of enlarging the category of belonging.

• BMB Reports
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• v.56 no.3
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• pp.190-195
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• 2023

We propose a novel blood biomarker detection method that uses miRNA super-resolution imaging to enable the early diagnosis of Alzheimer's disease (AD). Here, we report a single-molecule detection method for visualizing disease-specific miRNA in tissue from an AD mice model, and peripheral blood mononuclear cells (PBMCs) from AD patients. Using optimized Magnified Analysis of Proteome (MAPs), we confirmed that five miRNAs contribute to neurodegenerative disease in the brain hippocampi of 5XFAD and wild-type mice. We also assessed PBMCs isolated from the whole blood of AD patients and a healthy control group, and subsequently analyzed those samples using miRNA super-resolution imaging. We detected more miR-200a-3p expression in the cornu ammonis 1 and dentate gyrus regions of 3 month-old 5XFAD mice than in wild-type mice. Additionally, miRNA super-resolution imaging of blood provides AD diagnosis platform for studying miRNA regulation inside cells at the single molecule level. Our results present a potential liquid biopsy method that could improve the diagnosis of early stage AD and other diseases.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.271.3-272
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• 2003

Rhus verniciflua Stokes (RVS) is a widely used herbal plant with various biological properties. Our previous study using in vitro platelet aggregation in whole blood showed that the fractions of RVS had strong anti-aggregatory activity. In this study, using in vitro platelet aggregation in PRP and coagulation parameters, to investigate the anti-platelet activity and anticagulant effects of RVS ethyl acetate layer, the layer was subsequently fractionated by ODS columm chromatograph (50％ MeOH). (omitted)

• Korean Journal of Veterinary Research
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• v.18 no.1
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• pp.19-22
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• 1978

In order to isolate and identify Anaplasma bodies, two dairy cattle were inoculated with the whole blood of Korean cattle reacted to capillary tube agglutination test (Ana-test). The results obtained are as follows: 1. In the infected dairy cattle, Anaplasma bodies were first detected between 30 to 40 days post infection. 2. The isolates were identified as Anaplasma centrale on the basis of morphology of infected anaplasma bodies in erythocytes.

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.171.2-172
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• 2001