• Title/Summary/Keyword: white salt

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Isolation of Plasmids from the Moderately Halophilic Bacteria (Moderate 호염성 세균의 Plasmid 유전자 분리)

  • HONG Yong-Ki
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.18 no.6
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    • pp.557-562
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    • 1985
  • Moderately halophilic bacteria were collected from solar salt with Larsen medium containing $10\%$ NaCl. A total of 56 strains were isolated and tested for the presence of plasmid DNA by agarose gel electrophoresis. Twelve isolates ($21\%$) carried at least one kind of plasmid. Six different isolates among them were selected to study the molecular weight of plasmids and the morphological and physiological characters. Vibrio sp. 14, Alcaligenes sp. 63, Pseudomonas sp. 11, Flavobacterium sp. 38, Bacillus sp. 16, and Alcaligenes sp. 52 carried at least one plasmid of about 7.2 kbp, 6.4 kbp, 6.85 kbp, 8.5 kbp, 8.75 kbp, and 6.8 kbp respectively.

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A study on the cooking and processing methods presented in CHE MIN YO SUL ("제민요술"에 수록된 식품조리가공법 연구보고(6) -병.종.열.자명.예.로-)

  • 윤서석;윤숙경;조후종;이효지;안명수;안숙자;서혜경;윤덕인;임희수
    • Korean journal of food and cookery science
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    • v.7 no.1
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    • pp.81-85
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    • 1991
  • This study was carried out to understand and analyze the cooking and processing methods presented in CHE MIN YO SUL (chapter 82~86). The result from the study are as follows; 1. Byung was made from dough of wheat flour, glutinous rice flour, small green peas flour an rice of millet etc. by steaming, roasting, deep fat frying or sauted, or it had eaten Byung which was made from egg or seagull's egg without cereal was simillar to Korea's fried egg cake. 2. Jong and Youl were steamed product made from mixture of millet and rice which were wrapped with leaf of Julpool or bamboo's leaf. It's shape was simillar to Korea's Song-Pyun but different from materials and cooking method. 3. Jamyong's cooking method was boild the mixture of white rice soup, Myong-Jup, and salt. and then pour the rised Bal. 4. Ye and Lo were boiling product made from new oat and appricot seed flour. It was simillar to sweet drink made from rice, and put them in the jar for storage.

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Correlation Between Skin Irritation and Cytotoxicity of Anti-wrinkle Agents (화장품 원료의 피부자극성과 세포독성의 관련성)

  • 이은희;이종권;김용규;박기숙;안광수
    • YAKHAK HOEJI
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    • v.45 no.3
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    • pp.310-319
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    • 2001
  • To compare skin irritation and cytotoxicity of anti-wrinkle agents, we examined skin irritation of six anti-wrinkle agents (ascorbic acid, glycolic acid, all trans-retinoic acid, ginseng extract, retinol, EB) in New Zealand white rabbit. Cytotoxicity of these agents was determined by MTT [tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] at multi-time points in cultured HaCaT cell, a human immortalized keratinocyte cell. We then analyzed correlation between skin irritation and cytotoxicity by spearman's rank correlation analysis. All trans-retinoic acid showed the highest primary irritation index (0.92) in skin irritation test. Being all the six agents not irritant, retinal showed the most cytotoxic agents. The correlation between skin irritation and cytotoxicity ($IC_{50}$/ at different time point was 0.814, 0.757, 0.814 and 0.7 at 3, 24, 48 and 72 h, respectively. We also fecund that IC$_{20}$ and IC$_{80}$ of these agents showed similar correlation with skin irritation. These results therefore demonstrated that there is close correlation between skin irritation and cytotoxicity $IC_{50}$/ value by MTT in HaCaT cell at early time points by anti-wrinkle agents or IC$_{20}$ value. $IC_{50}$/ at earily time point or IC$_{20}$ values may be reliable alternative determinant of skin irritation.n.

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Effects of Hydrocolloids on the Quality of Protein and Transglutaminase Added Gluten-free Rice Bread (단백질과 트란스글루타미나제 첨가 글루텐 프리 쌀빵의 품질에 대한 친수콜로이드의 효과)

  • Hwang, Sun Ok;Kim, Ji Myoung;Shin, Malshick
    • Korean journal of food and cookery science
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    • v.33 no.2
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    • pp.198-208
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    • 2017
  • Purpose: To improve the quality of basic gluten-free rice bread composed of white rice flour, salt, sugar, yeast, skim milk powder, olive oil, and water, the effects of transglutaminase (TGase), whey protein (WP), propylene glycol alginate (PGA), and hydroxypropylmethylcelluose (HPMC) were investigated. Methods: TGase, WP, PGA, and HPMC were added to rice flour cumulatively. The pasting properties of rice flour blends as well as volume, shape, color value, textural properties and sensory evaluation of basic rice bread (RB1) RB1+TGase (RB2), RB1+TGase+WP (RB3), RB1+TGase+WP+PGA (RB4), and RB1+TGase+WP+PGA+HPMC (RB5) were compared. Results: Consistency of rice batter increased upon addition of TGase, WP and PGA, and RB3 and RB4 had higher specific volumes than others. PGA improved volume, crumb air cell uniformity, and resilience but lowered elasticity and moistness of RB. HPMC increased, hardness, moistness and softness, and slightly reduced volume. Conclusion: Therefore, it is suggested that hydrocolloids, PGA and HPMC may be necessary to improve volume, crumb structure, textural properties and overall eating quality of gluten-free rice bread.

Clinical Effects of an Improved Pump Reaction Rate and Automatic Occlusion Sensing System in Phacoemulsification (수정체유화장치의 초음파 출력속도 및 자동막힘감지 기능 향상의 술 후 임상결과 비교)

  • Kim, You Na;Lee, Jin Ah;Kim, Jae Yong;Kim, Myoung Joon;Tchah, Hung Won
    • Journal of The Korean Ophthalmological Society
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    • v.59 no.11
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    • pp.1017-1023
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    • 2018
  • Purpose: A recently introduced phacoemulsification system, the WhiteStar $Signature^{(R)}$ PRO, has demonstrated improved nucleus followability and cutting efficiency via an improved pump regulator with a higher reaction response and an automatic occlusion sensing system. In this study, we compared various phacoemulsification parameters between the new system and an older version of the device. Methods: A total of 80 eyes of 68 patients with cataracts who had undergone phacoemulsification by a single surgeon were included in this study. Forty eyes of patients underwent phacoemulsification using the older $Signature^{(R)}$ system (WhiteStar); these patients were classified as the control group. Another 40 eyes of patients underwent phacoemulsification with the newer enhanced system, the WhiteStar $Signature^{(R)}$ PRO; these patients were assigned to the experimental group. During the operation, operative parameters, including the effective phaco time (parameter of effective phaco time with a specific coefficient for the transversal movement expressed in seconds, EFX), ultrasound time (seconds [s]), effective phacoemulsification time (EPT, s), average phacoemulsification power (AVG, %), and balanced salt solution usage, were measured to determine the performance enhancement offered by the updated system. Central corneal thickness was measured before and after surgery to compare corneal edema. The relationships between the two groups were analyzed using an independent t-test. Results: The Signature $PRO^{(R)}$ system showed a lower EFX (p < 0.001), a shorter EPT (p < 0.001), and a smaller AVG (p < 0.001). Postoperative corneal thickness did not differ significantly between the two groups. Conclusions: Comparing the efficacy of the improved reaction speed of the device and automatic occlusion sensing system in performing phacoemulsification, the updated Signature $PRO^{(R)}$ system demonstrated superior followability and cutting efficiency regardless of nuclear cataract hardness.

A Study on Synthesis of Ca and Mg Compounds from Dolomite with Salt Additional React (MgCl2·6H2O) (염 첨가 반응(MgCl2·6H2O)을 이용하여 백운석으로부터 Ca 화합물과 Mg 화합물 합성에 관한 연구)

  • Hwang, Dae Ju;Yu, Young Hwan;Cho, Kye Hong;Lee, Jong Dae
    • Korean Chemical Engineering Research
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    • v.59 no.3
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    • pp.399-409
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    • 2021
  • In order to utilize dolomite as a calcium/magnesium compound material, it was prepared highly reactive calcined dolomite(CaO·MgO) using a microwave kiln (950 ℃, 60 min). The experiment was performed according to the standard of the hydration test (ASTM C 110) and hydration reactivity was analyzed as medium reactivity (max 74.1 ℃, 5 min). Experiments were performed with calcined dolomite and salt (MgCl2·6H2O) (a) 1:1, (b) 1:1.5, and (c) 1:2 wt% based on the hydration reaction of calcined dolomite. The result of X-ray diffraction analysis confirmed that MgO of calcined dolomite increased to Mg(OH)2 as the salt addition ratio increased. After the separating reaction, calcium was stirred at 80 ℃, 24 hr that produced CaCl2 of white crystal. XRD results, it was confirmed calcium chloride hydrate (CaCl2·(H2O)x) and CaO of calcined dolomite and salt additional reaction was separated into CaCl2. And it was synthesized with Ca(OH)2 99 wt% by NaOH adding reaction to the CaCl2 solution, and the synthesized Ca(OH)2 was manufactured CaO through the heat treatment process. In order to prepare calcium carbonate, CaCO3 was synthesized by adding Na2CO3 to CaCl2 solution, and the shape was analyzed in cubic form with a purity of 99 wt%.

Nutrients and Dish Intake by Fasting Blood Glucose Level (혈당 수준에 따른 영양섭취 및 음식섭취 상태)

  • Choi, Ji-Hyun;Moon, Hyun-Kyung
    • Journal of Nutrition and Health
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    • v.43 no.5
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    • pp.463-474
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    • 2010
  • The nutrient intake and association between dish group intake and blood glucose and serum lipid level (TG, cholesterol, LDL and HDL) was analyzed among 3 groups: 452 subjects in normal blood glucose group (NG: fasting blood glucose < 100 mg/dL and 2 hours postprandial blood glucose < 140 mg/dL), 258 subjects in impaired fasting glucose group (IFG: fasting blood glucose 100~125 mg/dL and 2 hours postprandial blood glucose ${\geq}$ 140 mg/dL) and 101 subjects in diabetic group (DG: fasting blood glucose $\geq$ 126 mg/dL and 2 hours postprandial blood glucose ${\geq}$ 140 mg/dL). The data were obtained from the 2005 National Health and Nutrition Survey of Korea. The 811 subjects were adults aged 40~64 without dietary treatment. In nutrients intake, IFG was the highest and DG the lowest in both quantity and quality. DG, especially, had the lowest intake in carbohydrates, fiber, proteins, Ca, P, K, vitamins B1 and C, and consumed the highest amount of alcohol. In macronutrients distribution ratio, the DG diet showed a lower energy intake from carbohydrates but higher from fat than the NG diet, while IFG showed a higher energy intake from carbohydrates and lower intake from fat in supper out of 3 meals and snacks. IFG preferred salt-fermented foods and DG preferred soups, braised foods and kimchi compared to other groups. NG preferred multi-grain cooked rice and both IFG and DG preferred plain white cooked rice. Regarding the association between dish group intake and blood glucose, cooked rice, soups, salt-fermented foods and kimchi were significantly related to blood glucose. In blood lipids, steamed-foods, beverages and fruits were inversely related to the risk of developing type 2 diabetes, whereas cooked rice, stews, saltfer-mented foods, seasoned-fermented foods and seasoned vegetables were directly proportional to the risk of developing type 2 diabetes and related diseases. Therefore, it is beneficial to avoid rich, salty and fatty foods and heavy alcohol consumption for controlling blood glucose and blood lipids, while steamed foods, foods rich in fiber (like multigrain rice) as a staple, and fruits and teas are recommended for preventing or managing type 2 diabetes risks.

Studies on the Salt Movement of Upland Soil in Reclaimed Tidal Land (간척지(干拓地) 밭토양(土壤)의 염류이동(鹽類移動)에 관(關)한 연구)

  • Lee, Jong-Sik;Kim, Jong-Gu;Yoo, Chul-Hyun;Kang, Jong-Gook;Kim, Ho-Jung
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.1
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    • pp.28-34
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    • 1991
  • This study was carried out to investigate the salt movement in upland soils in reclaimed region (Kye-Hwa Do). The results obtained were summarized as follows : 1. The cation content in ground water increased during time course. but in the case of land-surface water the content was variable, and $K^+$ was lower than that of $Na^+$ and $M^{+2}$. At the L. S. P(Low salinity plot) under rainproof condition, the salinity was directly proportional to soil water content, but at the H. S. P (High salinity plot) the tendency was no reversed. 3. In condition of rainproof, the amount of available phosphorous was higher at the H. S. P than at the L. S. P. 4. Positive correlation was obtained between the soil water content and available phosphorous content at the rainfall plot, but there was no significance at the surface soil of the rainproof plot. 5. SAR (Sodium adsorption ratio) and anion ($Cl^-$, $SO_4{^{2-}}$) contents in soil were repressed in the order of black vinyl>white vinyl>rice straw>control.

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Studies on the Interaction of Biocides and Ethylsilicate Consolidants for Stone Monument (석조문화재 살생물제와 에틸실리게이트 강화제의 상호작용에 관한 연구)

  • Do, Jin-Young;Yun, Yun-Kyung;Lee, Tae-Jong;Kyung, Hye-Sun
    • Journal of Conservation Science
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    • v.21
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    • pp.73-88
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    • 2007
  • In this paper, the interaction between five biocides(commercial) and two ethylsilicate consolidants for stone monuments, reacted in different sequence, has been studied. Through the structures, weight and gelation time of mixture of biocides and consolidants have been evaluated the reactivity of biocide alone, the reactivity of consolidants and biocides, the reactivity of consolidants and dried biocide, and the reactivity of ethylsilicate gel and biocides. The tested biocides show quite different properties from those of consolidants; after evaporation, some biocides are remained white salt crystals, another need the long time for evaporation and one biocide shows pale brown color. The results have shown an interaction of the tested products each other in some application sequences of the products. When the application of liquid state biocides with consolidants, it was noted that some biocide seem to interfere with the formation of gel due to reaction of consolidants and water and salts in biocides. In the reaction of ethylsilicate with dried biocides have shown a heterogenous gel(transparent layer with ethylsilicate alone and white layer which is mixed biocide and ethylsilicate) and many cracks in product due to the different shrinkage, thus the products don't play a role as consolidants. There is no change in structures and color in reaction of the gas state biocide and ethylsilicate gel.

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Stabilizing and Optimizing Properties of Crude Protease Extracted from Korean Figs (국내산 무화과에서 추출한 protease 조효소액의 안정성과 최적화에 관한 연구)

  • Kim, Mi-Hyun;Rho, Jeong-Hae;Kim, Mee-Jeong
    • Korean journal of food and cookery science
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    • v.27 no.3
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    • pp.29-37
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    • 2011
  • Protease activity of fig (Ficus carica L.), cultivated in Korea was estimated. In particular, the proteolytic effect on myofibrilar protein was studied. A crude protease extract of fig was prepared in two ways; fig was homogenized in buffer followed by centrifugation, and the supernatant was precipitated by saturated ammonium sulfate followed by dialysis. The former method resulted in 41.15 mM/g fig protease activity, whereas the latter method resulted in 17.65 mM/g fig protease activity. The crude fig protease extract showed high specificity for casein as a substrate followed by egg white, bovine serum albumin, myofibrilar protein, collagen, and elastin. The extract had stable proteolytic activity in a pH range of 6.5~9.0 (optimal at pH 7-8) but lost activity, at pH 2-3. Proteolytic activity for myofibrilar protein was sensitive to pH. The proteolytic activity of the fig extract was steady up to $60^{\circ}C$ but declined at higher temperature. It also began to lose stability in salt concentrations >0.7 M NaCl. Fig has been used as a meat tenderizer for cooking, and these results support the tenderizing effectiveness of fig, particularly for Korean style meat marinating.