• Mycobiology
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• 제33권4호
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• pp.240-242
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• 2005

A soft rot of fruits caused by Mucor racemosus occurred on cherry tomato collected in Agricultural Products Wholesale Market in Jinju, Korea. The disease infection usually occurred wounded areas after cracking of fruits. At first, the lesions started with water soaked and rapidly softened and diseased lesion gradually expanded. Colonies were white to brownish to gray in color. Sporangia were $32{\sim}54\;{\mu}m$ in size and globose in shape. Sporangiophores were $8{\sim}14\;{\mu}m$ in width. Sporangiospores were $5{\sim}12\;{\times}\;4{\sim}8\;{\mu}m$ in size, ellipsoidal to subglobose in shape. Columella was $27{\sim}42\;{\mu}m$ in size, obovoid, ellipsoidal, cylindrical-ellipsoidal, slightly pyriform in shape. Chlamydospores were numerous in sporangiophores and barrel-shaped when young, subglobose in old cultures. Optimum growth temperature was about $25^{\circ}C$. The fungus was identified as M. racemosus Fres.. This is the first report of soft rot on cherry tomato caused by M. racemosus in Korea.

• Mycobiology
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• 제40권3호
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• pp.214-216
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• 2012

Soft rot on banana fruit caused by Rhizopus oryzae was identified for the first time in Korea. Colonies were white to light brown and formed numerous sporangiospores. Optimum temperature for mycelial growth was $30^{\circ}C$. Sporangia were globose and $30{\sim}200{\mu}m$. Sporangiophores were usually straight, $8{\sim}20{\mu}m$, and rhizoids usually in groups of 3~5. Columella were globose to sub-globose and $90{\sim}110{\mu}m$. Sporangiospores were sub-globose or oval and $4{\sim}10{\mu}m$. Based on its mycological characteristics, molecular analysis, and pathogenicity to host plants, this fungus was identified as Rhizopus oryzae Went & Prisen Geerligs. This is the first report of soft rot on banana caused by Rhizopus oryzae in Korea.

• Mycobiology
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• 제39권3호
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• pp.230-232
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• 2011

In this study, we characterized sporadically occurring sclerotium rot caused by Sclerotium rolfsii in Chinese chive (Allium tuberosum Roth.) in farm fields in Sacheon, Korea. The initial symptom of the disease was water-soaked, which progressed to rotting, wilting, blighting, and eventually death. Further, mycelial mats spread over the lesions near the soil line, and sclerotia formed on the scaly stem and leaves. The sclerotia were globoid, 1~3 mm, and white to brown. The optimum temperature for growth and sclerotia formation on potato dextrose agar (PDA) was $30^{\circ}C$. The diameter of the hypae ranged from 4 to 8 ${\mu}m$. Clamp connection was observed on PDA medium after 5 days of incubation. Based on the mycological characteristics, internal transcribed spacer sequence analysis, and pathogenicity test, the causal agent was identified as Sclerotium rolfsii Saccardo. This is the first report of sclerotium rot in Chinese chive caused by S. rolfsii in Korea.

• Mycobiology
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• 제38권2호
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• pp.156-158
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• 2010

Stem rot disease was found in garlic (Allium sativum L.) cultivated from 2008 to 2010 in the vegetable gardens of some farmers in Geumsan-myon, Jinju City, Gyeongnam province in Korea. The initial symptoms of the disease were typical water-soaked spots, which progressed to rotting, wilting, blighting, and eventually death. White mycelial mats had spread over the lesions near the soil line, and sclerotia had formed over the mycelial mats on the stem. The sclerotia were globoid in shape, 1~3 mm in size, and tan to brown in color. The optimum temperature for growth and sclerotia formation on potato dextrose agar (PDA) medium was $30^{\circ}C$. The diameter of the hyphae ranged from approximately 4 to $8\;{\mu}m$. Typical clamp connection structures were observed in the hyphae of the fungus, which was grown on PDA medium for 4 days. On the basis of the mycological characteristics and pathogenicity of the fungus on the host plants, the causal agent was identified as Sclerotium rolfsii Saccardo. This is the first report of stem rot disease in garlic caused by S. rolfsii in Korea.

• 한국응용곤충학회지
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• 제25권2호
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• pp.63-70
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• 1986

1981년도 사과 흰빛 썩음병(白腐病)의 발생은 지역에 따라 5%에서 16%에 달하였으며 평균발병 과율은 9%였다. 사과에서 분리한 병원균을 사과, 배, 복숭아, 포도의 잎, 가지, 과실에 상처 접종하였을때 접종 부위에 병반이 형성되었다. 병원균의 영양 생장은 감자설탕 한천배지와 귀리즙 한천배지에서 모두 좋았으며 특히 온도 $25{\sim}30^{\circ}C$, pH 4, 당도 $10{\sim}15%$의 광조사하에서 가장 양호하였다. 사과의 이병과실은 8월 초순에 포장에서 처음 관찰되었으며 이때의 과실 당도는 9.0%였다. 그 이후 과실의 당도가 증가함에 따라 이병과실의 수도 급격히 증가하였다. 과실의 이병정도와 과일즙내 산도와는 아무런 상관이 없었다. 사과 과실의 이병시기를 과실을 일정기간동안 노출하는 방법으로 포장에서 조사한 결과, 과실의 이병은 과실이 형성되기 시작한 6월초순부터 가능하였다. 과실의 숙기별로 포장에서 과실을 채집하여 실내 유상접종 하였을때 접종 과실은 과실의 숙기와 상관없이 모두 병만을 형성하였다. 포장에서 병 발생 정도를 품종별로 조사한 결과, 골덴데리셔스, 육오가 가장 많이 이병되었고 조나단, 레드데리셔스가 가장 적었으며 아오리, 후지, 인도는 중간이었다.

• 한국자원식물학회지
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• 제29권1호
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• pp.39-45
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• 2016

2012년부터 2013년에 걸쳐 식물체 근권과 비근권 토양을 수집하였다. 수집한 토양을 희석평판법을 이용하여 총 782개의 세균을 분리하였고, 분리한 세균을 실험실내에서 Trichothecium roseum과의 대치배양을 통해 균사생장억제율이 80% 내외로 우수한 길항력을 나타내는 균주를 선발하였다. I-plate에서 HK2균주와 T. roseum두 균을 격리하여 밀폐배양 시 모두 균사생장억제 효과는 없기 때문에 휘발 물질은 아닌 것으로 판단되었다. NB배지에 길항균주와 T. roseum을 액체배양 한 결과 88% 이상 균사생장억제 효과를 보였다. HK2균주를 동정하기 위해 16S rDNA 염기서열분석과 API 50 CHB Kit (BioMerieux, France)를 이용하여 생화학적 특성을 분석한 결과 Bacillus subtilis로 동정되었다. HK2 균주가 생산한 항진균물질을 butanol로 추출한 후 flash column chromatography를 이용하여 항진균물질을 정제한 결과 methanol 80%의 조건에서 잘 분리되었으며 향후 분리 순화를 통한 화학구조분석이 필요하다.

• 한국미생물·생명공학회지
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• 제24권3호
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• pp.261-267
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• 1996

To examine the potential utilization of chitosan, the biodegradable natural polymer, as a control agent of apple white rot caused by Botryosphaeria dothidea in a new control measure by coating it on the diseased branches, the various antifungal activities of chitosan was investigated. Chitosan showed significant inhibitory effect on the mycelial growth of B. dothidea, along with the morphological changes including hyphal swelling and ultrastructural changes on solid PDA medium. In liquid PD broth medium, the chitosan showed more significant effect on the growth of B. dothidea also forming cell clusters indicating affection on the hyphal extension. The growth of B. dothidea was inhibited more than 90% at the concentration of 1.0 mg/ml. Chitosan also detained the spore germination and induced the morphological change of germ tubes. Glucosamine, monomer of chitosan, did not affect on the growth of B. dothidea indicating the antifungal activity was caused by chitosan polymer.

• 식물병과 농업
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• 제1권1호
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• pp.37-44
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• 1995

This study was carried out to select effective fungicides against white and violet root rot caused by Rosellinia necatrix and Helicobasidium mompa with nine fungicides including thiophanate-methyl from 1993 to 1994. Through laboratory, greenhouse and field trials on inhibitory effect of mycelial growth and disease incidence against the two fungal pathogens, 5 fungicides have been selected finally. Thiopanate-methyl, benomyl, iminoctadine-triacetate and isoprothiolane were proven to have high control effect against R. necatrix. In addition to thiopanate-methyl and benomyl, tolclofos-methyl has been selected for effective control of H. mompa, since it showed prominent control effect in field trial than in laboratory or green house test.

• Journal of Microbiology
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• 제42권1호
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• pp.37-41
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• 2004

The textile industry wastewater has been decolorized efficiently by the white rot fungus, Irpex lacteus, without adding any chemicals. The degree of the decolorization of the dye effluent by shaking or stationary cultures is 59 and 93%, respectively, on the 8th day. The higher level of manganese-dependent peroxidase (MnP) and non-specific peroxidase (NsP) was detected in stationary cultures than in the cultures shaken. Laccase activities were equivalent in both cultures and its level was not affected significantly by the culture duration. Neither lignin peroxidase (LiP) nor Remazol Brilliant Blue R oxidase (RBBR ox) was detected in both cultures. The absorbance of the dye effluent was significantly decreased by the stationary culture filtrate of 7 days in the absence of Mn (II) and veratryl alcohol. In the stationary culture filtrate, three or more additional peroxidase bands were detected by the zymogram analysis.

• 한국환경보건학회지
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• 제32권4호
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• pp.381-385
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• 2006

For decolorization of synthetic dyes, One fungus(HUE05-1) which was isolated from textile wastewater collected from industrial complex in Korea showed excellent ability of removing synthetic dyes. This fungus was identified as Basidiomycetes species by Internal Transcribed Spacers (ITS) sequence. Isolated fungi. HUE05-1 completely decolorized all dyes in both solid and liquid condition. The decolorization results were Reactive Orange-16, 97.12%; Reactive Blue-19, 92.09%; Reactive Blue-49, 97.04%; Reactive Yellow-145, 95.53%; Acid Orange-10, 99.18%; Acid Violet-43, 98.73%; Acid Blue-350, 94.71% and Disperse Blue-106, 90.07%.