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Optimal Incorporation Level of Dietary Alternative Phosphate (MgHPO4) and Requirement for Phosphorus in Juvenile Far Eastern Catfish (Silurus asotus)

  • Yoon, Tae-Hyun;Lee, Dong-Hoon;Won, Seung-Gun;Ra, Chang-Six;Kim, Jeong-Dae
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.1
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    • pp.111-119
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    • 2015
  • A growth trial was conducted to determine the optimal incorporation level of dietary magnesium hydrogen phosphate (MHP, $MgHPO_4$), which was manufactured from swine manure and phosphorus (P), required by juvenile far eastern catfish (Silurus asotus). Graded MHP of 0.5%, 1.0%, 1.5%, and 2.0%, and 2.0% monocalcium phosphate (MCP) each was added to the basal diet (control) in lieu of cellulose to become the range of available P (AP) from 0.4% to 0.8% of which diets were designated as control, MHP0.5, MHP1.0, MHP1.5, MHP2.0, and MCP, respectively. Control diet contained fish meal (20%), soybean meal (40%), wheat flour (27%), corn gluten meal (5%), fish oil (2%) and soy oil (2%) as main ingredients. Following a 24 h fasting, 540 fish with a mean body weight of 11.8 g were randomly allotted to 6 groups in triplicate, whereby 18 tanks ($0.4{\times}0.6{\times}0.36cm$, water volume of 66 L) were prepared. The feeding experiment lasted for 8 weeks. Fish group fed the control diet showed the lowest weight gain (WG) and feed efficiency (FE) among treatments. The WG was, however, not significantly different (p>0.05) from that of fish group fed MHP0.5. Fish group fed MHP2.0 showed the highest WG and FE of which values were not significantly different from those of fish groups fed diets MHP1.0 and MHP1.5 as well as MCP (p>0.05) except fish groups fed control and MHP0.5. Aspartate aminotransferase was significantly decreased with an increase in available P, while alanine aminotransferase did not show a significant difference among treatment. The highest inorganic P in plasma was observed in fish fed MHP2.0. From the present results, a second-order regression analysis revealed that the optimal dietary MHP level and the AP requirement were found to be 1.62% and 0.7%, respectively.

Effect of condensed tannins from Ficus infectoria and Psidium guajava leaf meal mixture on nutrient metabolism, methane emission and performance of lambs

  • Pathak, A.K.;Dutta, Narayan;Pattanaik, A.K.;Chaturvedi, V.B.;Sharma, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.12
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    • pp.1702-1710
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    • 2017
  • Objective: The study examined the effect of condensed tannins (CT) containing Ficus infectoria and Psidium guajava leaf meal mixture (LMM) supplementation on nutrient metabolism, methane emission and performance of lambs. Methods: Twenty four lambs of ~6 months age (average body weight $10.1{\pm}0.60kg$) were randomly divided into 4 dietary treatments (CT-0, CT-1, CT-1.5, and CT-2 containing 0, 1.0, 1.5, and 2.0 percent CT through LMM, respectively) consisting of 6 lambs each in a completely randomized design. All the lambs were offered a basal diet of wheat straw ad libitum, oat hay (100 g/d) along with required amount of concentrate mixture to meet their nutrient requirements for a period of 6 months. After 3 months of experimental feeding, a metabolism trial of 6 days duration was conducted on all 24 lambs to determine nutrient digestibility and nitrogen balance. Urinary excretion of purine derivatives and microbial protein synthesis were determined using high performance liquid chromatography. Respiration chamber study was started at the mid of 5th month of experimental feeding trial. Whole energy balance trials were conducted on individual lamb one after the other, in an open circuit respiration calorimeter. Results: Intake of dry matter and organic matter (g/d) was significantly (p<0.05) higher in CT-1.5 than control. Digestibility of various nutrients did not differ irrespective of treatments. Nitrogen retention and microbial nitrogen synthesis (g/d) was significantly (p<0.01) higher in CT-1.5 and CT-2 groups relative to CT-0.Total body weight gain (kg) and average daily gain (g) were significantly (linear, p<0.01) higher in CT-1.5 followed by CT-1 and CT-0, respectively. Feed conversion ratio (FCR) by lambs was significantly (linear, p<0.01) better in CT-1.5 followed by CT-2 and CT-0, respectively. Total wool yield (g; g/d) was linearly (p<0.05) higher for CT-1.5 than CT-0. Methane emission was linearly decreased (p<0.05) in CT groups and reduction was highest (p<0.01) in CT-2 followed by CT-1.5 and CT-1. Methane energy (kcal/d) was linearly decreased (p<0.05) in CT groups. Conclusion: The CT supplementation at 1% to 2% of the diet through Ficus infectoria and Psidium guajava LMM significantly improved nitrogen metabolism, growth performance, wool yield, FCR and reduced methane emission by lambs.

Study on Medium Ingredient Composition for Enhancing Biomass Productionand Anti-potato Common Scab Activity of Streptomyces sp. A020645 as a BCA Candidate (생물제제(BCA) 후보균주인 Streptomyces sp. A020645 의 대량 균체생산 및 항더뎅이병 활성증진을 위한 고체배지 조성에 관한 연구)

  • Lee, Hyang-Burm;Roh, Hyo-Young;Park, Dong-Jin;Lee, So-Keum;Ko, Young-wan;Koh, Jeong-Sam;Kim, Chang-Jin
    • Research in Plant Disease
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    • v.11 no.1
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    • pp.66-71
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    • 2005
  • The effect of medium components such as wheat bran, rice bran, oat meal, and soybean meal as basic ingredients and KH2PO4, glucose, and molasses as additives on mass production and anti-potato common scab activ ity of a streptomycete A020645 strain as a biocontrol agent (BCA) candidate was investigated. Of basicingredients, oat meal was the best one for mass poduction and enhancement of anti-potato common scabactivity. The biomass production of the active strain was more enhanced when 0.1-0.01.% glucose or molassesas additive were added into the basic medium. These information may have important implications in applying for effective formulation of BCA.

Silkworm (Bombyx mori) Response to Differently Formulated Artificial Diets

  • Mottaghitlab, M.;Pourali, M.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.2
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    • pp.207-210
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    • 2004
  • Artificial diet (AD) has shown with different advantages over mulberry leaves (ML). Various types of such feed for silkworm have been developed and proposed. The aims of this study were possibility rearing whole instar of silkworm on AD and to find some economic formulation for rearing silkworm, compare to that ML. Eleven ADs (D$_1$ to D$_{11}$) were prepared with different percentage of mulberry leaf powder, and other ingredients such as soybean meal, cellulose, potato starch, agar, wheat flour, rice bran, etc. Five formulated diets (D$_1$ D$_2$, D$_{5}$, D$_{10}$ and D$_{11}$) appeared to have similar response to that ML (D$_{m}$). There were no significant differences for duration period between ADs, all of which recorded with higher duration than mulberry leaves. Compared to other died used in the present study D$_1$ and D$_{10}$ showed better growth development and survival rate. Larvae fed D$_1$, D$_2$, D$_{5}$, D$_{10}$ and D$_{11}$ grew faster and produced heavier and more useful cocoons.coons.coons.coons.

SELECTION OF VISIBLE/NIR WAVELENGTHS FOR CHARACTERIZING FECAL AND INGESTA CONTAMINATION OF POULTRY CARCASSES

  • William R.Windham;Park, Bosoon;Kurt C.Lawarece;Douglas P.Smith
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.3105-3105
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    • 2001
  • Ingests and fecal contamination on a poultry carcass is a food safety hazard due to potential microbiological contamination. A visible/near-infrared (NIR) spectrometer was used to discriminate among pure ingesta and fecal material, breast skin contaminated with ingesta or fecal material and uncontaminated breast skin. Birds were fed isocaloric diets formulated with either maize, mile, or wheat and soybean meal for protein requirements. Following completion of the feeding period (14 days), the birds were humanely processed and eviscerated to obtain ingests from the crop or proventriculus and feces from the duodenum, ceca, and colon portion of the digestive tract. Pure feces and ingesta, breast skin, and contaminated breast skin were scanned from 400 to 2500 nm and analyzed from 400 to 900 nm. Principal component analysis (PCA) of reflectance spectra was used to discriminate between contaminates and uncontaminated breast skin. Results indicate that visible (400 to 760 nm) and NIR 760-900 nm spectra can detect contaminates. From PCA analysis, key wavelengths were identified for discrimination of uncontaminated skin from contaminates based the evaluation of loadings weights.

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Evaluation of Feeding a Fibrolytic Enzyme to Lactating Dairy Cows on Their Lactational Performance during Early Lactation

  • Titi, H.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.5
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    • pp.677-684
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    • 2003
  • Twenty eight multiparous lactating cows were utilized in an experiment to evaluate the response to an exogenous fibrolytic enzyme on their lactational performance during early lactation period (in terms of milk production, milk composition, feed intake, milking efficiency, body weight change) and the exact time of this response. Cows were randomized into two groups (14 each) with similar parities and were fed a concentrate ration of barley, ground corn, soybean meal, and wheat bran and roughage ration of alfalfa hay. One of the two groups was supplemented with the fibrolytic enzyme immediately after parturition up to 100 post partum. The experiment was of two phases with 50 days each. The enzyme, which has a cellulase/hemicellulase activity (derived from Trichoderma group), was added to the concentrate part of the ration in a dry powder form. Milk production, 3.5% fat corrected milk, energy corrected milk were higher (p<0.05) for cows fed treated diet. At the same time, No differences were observed in percentages of milk components, feed intake, body weight, body weight change, or rectal temperature for the whole experimental period or during any of the two phases. Efficiency of milk production was higher (p<0.05) for treatment group cows than for that of the control ones. However, efficiency was better during the second phase than during the first phase. Feeding enzyme treated diets to dairy cows improved lactational performance during early 100 day of the lactation period. However, the first 50 days of lactation looked to be the critical.

Biological performance evaluation of tubular subsurface cage system for sea cucumber, Apostichopus japonicus, grow-out by in-situ tests (해상 실험에 의한 침하식 해삼 양성 기구의 생물학적 성능 평가)

  • Oh, Moo-Hwan;Kwon, Inyeong;Kim, Taeho
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.50 no.2
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    • pp.202-213
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    • 2014
  • The sea cucumber, Apostichopus japonicus, is becoming an important aquaculture species in China, Japan and Korea. The purpose of this study is to evaluate the biological performance of a tubular type of subsurface cage for sea cucumber grow-out by in-situ tests. The cage structure was constructed of PP (polypropylene) frames and PP tubes (${\phi}130mm{\times}24pieces$). A set of tests were conducted at a depth of 15 m near Seosaeng, Ulsan, Korea for 295 days (23 July, 2012 to 13 May, 2013). A total of 155 sea cucumbers were used and fed a mixed diet containing mud, mineral, wheat, fish meal, etc (3% of their body weight). At the end of the experiments, sea cucumber showed a higher survival rate in feed shelter (77.14%) rather than no feed shelter (64.71%). The specific growth rate (SGR) of sea cucumbers in feed condition (0.04% $day^{-1}$) was higher than that of no feed culture animals (-0.49% $day^{-1}$) during total experimental periods. The result was able to find a no significance difference in survival rate but a significance difference in SGR under feed and no feed treatments.

Effect of Breads Containing Trans Fat, Soybean or Rice on Plasma Lipid and Fatty Acid Composition in Healthy Korean Adults (Trans 지방과 쌀, 콩을 첨가한 빵의 섭취가 건강한 성인 남녀의 혈장 지질 및 지방산 조성에 미치는 영향)

  • Noh, Kyung-Hee;Huh, Young;Jang, Ji-Hyun;Kim, Soo-Hee;Shin, Jin-Hyuk;Kim, Do-Hoon;Lee, Seong-Hwan;Lee, Kyung-Sik;Park, Yong-Kyu;Cho, Kyung-Hwan;Song, Young-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.8
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    • pp.1042-1049
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    • 2009
  • It has been reported that trans fat (tFA) may have adverse or beneficial effect depending upon the position and number of double bonds. The presence of tFA in human tissues and fluids is related to dietary intake, intestinal absorption, metabolism and storage, exchanges among compartments. This study investigated the effect of breads containing tFA, soybean or rice on postprandial plasma fatty acid and lipid composition. 33 healthy volunteers were divided into 3 groups and fed soybean bread, rice bread or wheat bread groups containing equivalent amounts of tFA (elaidic acid rich, 3.75 g/day), respectively. Postprandial lipid profiles at 0, 1, 2, 3 and 4 hours after a respective meal were studied. Plasma fatty acid was extracted by the method of Folch and methyl ester of fatty and prepared by acid transmethylation and analyzed by Gas Chromatography. Peaks were identified using pure reference compounds and quantified. Postprandial data indicated that consumption of soybean and rice breads with 3.75 g tFA retarded the appearance of C18:1 and C18:2 tFA in plasma lipid compared to that of wheat bread. Futhermore, soybean and rice bread groups showed lower plasma saturated fatty acid levels than wheat bread group. Postprandial TG level was significantly lowered in soybean bread group compared to that of rice and wheat bread groups. These results imply that soybean bread with high dietary fiber content and biologically active substances may inhibit or delay lipid absorption.

Effects of L-carnitine, Selenium-enriched Yeast, Jujube Fruit and Hwangto (Red Clay) Supplementation on Performance and Carcass Measurements of Finishing Pigs

  • Han, Yung-Keun;Thacker, P.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.2
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    • pp.217-223
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    • 2006
  • Fifty castrated crossbred ($Landrace{\times}Yorkshire$) pigs, weighing an average of $60.6{\pm}3.1kg$ were allotted to one of five treatments in a randomized block design to examine the effects of dietary inclusion of 0.1% L-carnitine (50 ppm carnitine), 0.1% selenium-enriched yeast (0.3 ppm selenium), 0.1% Jujube fruit or 0.1% Hwangto (Red clay) on pig performance and carcass quality. All diets were based on corn, wheat, soybean meal and wheat bran and were formulated to supply 13.8 MJ DE/kg. Dietary supplementation did not influence daily gain (p = 0.57), feed intake (p = 0.52), or feed conversion (p = 0.32). Digestibility of dry matter (p = 0.60), organic matter (p = 0.74), crude protein (p = 0.76), crude fibre (p = 0.70) and energy (p = 0.75) were also unaffected by inclusion of any of the additives. Tissue samples taken from the longissimus muscle showed that the levels of carnitine (p = 0.0001) and selenium (p = 0.0001) were significantly higher with dietary inclusion of carnitine or selenium-enriched yeast. Dietary treatment did not affect dressing percentage (p = 0.33), carcass lean yield (p = 0.99) or first, $10^{th}$ and last rib midline backfat depth (p = 0.45, 0.82 and 0.47, respectively). Dietary treatment also did not affect the percentages of tenderloin (p = 0.37), bacon (p = 0.36), fat and bone (p = 0.56), picnic shoulder (p = 0.25), skirt (p = 0.80), fresh ham (p = 0.31) or ribs (p = 0.79). However, pigs fed the diet containing Jujube fruit had a higher percentage of Boston butt than pigs fed the carnitine or selenium supplemented diets (p = 0.01). Pigs fed added Hwangto had a higher (p = 0.04) percentage of loin compared with pigs fed supplementary selenium or Jujube fruit. Loin muscle from pigs fed carnitine had a significantly lower Hunter colour value for L (whiteness, p = 0.004) and a higher value for $a^*$ (redness; p = 0.069). The overall results indicate that supplementation with L-carnitine and selenium-enriched yeast can produce pork containing higher levels of carnitine and selenium, which could provide health benefits for consumers of pork without detrimental effects on pig performance.

Production and Characterization of Ethanol- and Protease-Tolerant and Xylooligosaccharides-Producing Endoxylanase from Humicola sp. Ly01

  • Zhou, Junpei;Wu, Qian;Zhang, Rui;Yang, Yuying;Tang, Xianghua;Li, Junjun;Ding, Junmei;Dong, Yanyan;Huang, Zunxi
    • Journal of Microbiology and Biotechnology
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    • v.23 no.6
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    • pp.794-801
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    • 2013
  • This paper reports the production and characterization of crude xylanase from the newly isolated Humicola sp. Ly01. The highest (41.8 U/ml) production of the crude xylanase was obtained under the optimized conditions (w/v): 0.5% wheat bran, 0.2% $KH_2PO_4$, and 0.5% peptone; initial pH 7.0; incubation time 72 h; $30^{\circ}C$; and 150 rpm. A considerable amount of the crude xylanase was induced using hulless barley bran or soybean meal as the carbon source, but a small amount of the enzyme was produced when supplementary urea was used as the nitrogen source to wheat bran. The crude xylanase showed apparent optimal cellulase-free xylanase activity at $60^{\circ}C$ and pH 6.0, more than 71.8% of the maximum xylanase activity in 3.0-30.0% (v/v) ethanol and more than 82.3% of the initial xylanase activity after incubation in 3.0-30.0% (v/v) ethanol at $30^{\circ}C$ for 2 h. The crude xylanase was moderately resistant to both acid and neutral protease digestion, and released 7.9 and 10.9 ${\mu}mol/ml$ reducing sugar from xylan in the simulated gastric and intestinal fluids, respectively. The xylooligosaccharides were the main products of the hydrolysis of xylan by the crude xylanase. These properties suggested the potential of the crude enzyme for being applied in the animal feed industry, xylooligosaccharides production, and high-alcohol conditions such as ethanol production and brewing.