• International Journal of Oral Biology
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• 제47권1호
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• pp.9-15
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• 2022

Humulus japonicus (HJ) is a widely used herbal medicine for pulmonary tuberculosis, hypertension, leprosy, and venomous wounds in Asia, particularly in China. Although HJ has certain physiological activities, such as longitudinal bone growth, antioxidation and alleviation of rheumatism, its anticancer activities, other than in colorectal and ovarian cancer, are yet to be studied. In this study, we investigated the anti-cancer activity and mechanism of methanol extracts of HJ (MeHJ) against human FaDu hypopharyngeal squamous carcinoma cells. MeHJ suppressed FaDu cell viability without affecting normal cells (L929), which was demonstrated using the MTT and Live & Dead assays. Furthermore, MeHJ effectively inhibited colony formation of FaDu cells, even at non-cytotoxic concentrations, and significantly induced apoptosis through the proteolytic cleavage of caspase-9, -3, -7, poly (ADP-ribose) polymerase and through the downregulation of BCL-2 and upregulation of BAX in FaDu cells, as determined by DAPI staining, flow cytometry, and western blot analyses. Collectively, these findings suggest that the inhibitory effects of MeHJ on the growth and colony formation of oral cancer cells may be mediated by caspase- and mitochondrial-dependent apoptotic pathways in human FaDu hypopharyngeal squamous carcinoma cells. Therefore, MeHJ has the potential to be used as a natural chemotherapeutic drug against human oral cancer.

• International Journal of Oral Biology
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• 제46권4호
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• pp.160-167
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• 2021

Nypa fruticans Wurmb (NFW) contains a large amount of phenolic acid and flavonoids, and is popular as a superfood in Myanmar. NFW has various biological activities, such as anti-inflammatory, anti-oxidant, and neuroprotective properties; however, the anti-cancer effect of NFW have not been reported. In this study, we investigated the anticancer activity of water extracts of NFW (WeNFW) and the underlying mechanism in human FaDu hypopharyngeal squamous carcinoma cells. The WeNFW inhibited FaDu cell growth in a dose-dependent manner without affecting normal cells (L929), as determined by an MTT assay and Live and Dead assay. In addition, the concentrations of WeNFW without cytotoxicity (0.025, 0.05, and 0.1 mg/mL) inhibited wound healing and colony formation. Furthermore, WeNFW significantly induced apoptosis through the proteolytic cleavage of caspase-3 and -9, poly (ADP-ribose) polymerase, and downregulation of Bcl-2 and upregulation of Bax in FaDu cells, as determined by DAPI staining, FACS analysis, and western blot analysis. Taken together, these results suggest that WeNFW exhibits potent anti-cancer effects by suppressing the growth of oral cancer cells, wound healing and colony formation activity. Via mitrochondrial-dependent apoptotic pathways in human FaDu hypopharyngeal squamous carcinoma cells. Therefore, WeNFW can provide a natural chemotherapeutic drug for oral cancer in humans.

• 한국해양바이오학회지
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• 제15권2호
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• pp.33-40
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• 2023

This study aimed to investigate the immunomodulatory function of Pyropia yezoensis hydrothermal (water) extract (PYWE) in comparison to the group treated only with lipopolysaccharides (LPS) in RAW264.7 cells. LPS is known to be an inflammatory mediator that activates macrophages, leading to the secretion of nitric oxide (NO), inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) as defense responses. Through enzyme-linked immunoassay and western blot analyses, it was observed that PYWE increased the expression levels of NO, iNOS, TNF-α, and IL-6 in RAW264.7 cells in a dose-dependent manner, although to a lesser extent compared with the group treated with LPS alone. In addition, the study examined the mitogen-activated protein kinases (MAPKs) pathway, which regulates various cellular activities, including gene expression, mitosis, cell differentiation, transformation, survival, and death. The western blot analysis confirmed that PYWE also regulated the MAPKs pathway. Furthermore, the expression levels of immunomodulatory-related factors increased in the group treated with PYWE compared with the control group. Even though the effects of PYWE were usually less strong than those of LPS, the effects of PYWE increased with increasing doses compared to the control group. This suggests that PYWE could be used to control the immune system.

• 한국컴퓨터정보학회논문지
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• 제26권5호
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• pp.127-132
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• 2021

세계보건기구가 2007년도에 고령친화도시 조성에 대한 가이드라인을 제시한 이후에 전 세계적으로 고령화 사회에 대비하기 위해서 각 도시마다 고령친화도시 조성을 위한 노력들이 진행되고 있다. 이러한 시점에서 본 연구는 현재 고령친화도시로 인증 받은 자치단체와 인증을 준비하는 자치단체에 대해서 노인 1인 가구의 복지수준의 증진방안을 위한 정책적 시사점 제시를 본 연구의 목적으로 하였다. 이를 위해서 고령친화도시와 노인 1인 가구에 대한 이론적 논의를 토대로 정책적 시사점은 고령친화도시 조성의 8대 영역을 반영하는 1인 가구 노인의 주거사항, 경제사항, 건강사항, 여가 및 사회참여 사항, 등 네 가지 차원에서 정책적 반영을 제시하였다. 그리고 1인 가구 노인들의 미실태조사에 대한 한계점과 설문조사를 통한 향후 연구방향에 대해서 제시하였다.

• 한국컴퓨터정보학회논문지
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• 제27권11호
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• pp.199-207
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• 2022

본 연구는 근로능력이 노인의 우울에 미치는 영향을 도시와 농촌노인을 중심으로 비교 분석하여 노인근로정책과 정신건강정책 마련을 위한 자료제시에 연구목적을 두고 수행하였다. 연구를 위한 분석자료는 한국복지패널 16차년도 자료(2021년)를 이용하였고, 연구대상은 65세 이상 노인 4,838명의 자료를 발췌하여 분석에 투입하였다. 분석방법은 SPSS 22.0을 이용하여 기술통계, 회귀분석을 실시하였다. 연구결과 전체 노인은 근로능력이 있는 경우 우울이 감소하는 것으로 나타났다. 도시노인은 건강상태와 가구형태가 영향을 주는 것으로 나타났고 농촌노인은 건강상태와 교육수준이 영향을 주는 것으로 나타났다. 따라서 도시와 농촌지역의 자원과 환경에 적절한 지역 특색에 맞은 지역사회복지서비스가 제공이 요구되고 된다.

• 한국컴퓨터정보학회논문지
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• 제16권5호
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• pp.173-183
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• 2011

이 연구는 사회복지시설에서 클라이언트 폭력에 따른 시설종사자의 부정적 반응이 직무소진에 미치는 영향을 실증적으로 분석해 봄으로써 정책적 이론적 시사점을 도출한 연구이다. 특히 선행연구에서 매우 미흡하였던 인구통계적 변수와 시설 대응체계의 조절효과를 분석해 봄으로써 사회복지시설 종사자의 직무소진관리를 위한 시사점을 제시하는데 연구목적을 두었다. 이러한 연구목적을 달성하기 위해 G광역시와 J도에 소재하고 있는 사회복지시설 종사자들을 대상으로 표본추출하여 설문조사를 실시하였다. 이 연구의 분석결과 다음과 같은 시사점이 도출되었다. 첫째, 사회복지시설 종사자의 직무소진을 줄이기 위해서는 클라이언트 폭력에 대한 부정적 반응을 줄여야 하는 것으로 나타났다. 둘째, 클라이언트 폭력에 따른 종사자의 부정적 반응이 직무소진에 미치는 영향은 인구통계적 특성과 시설의 대응체계에 따라 다른 것으로 나타났다. 이 연구는 이러한 분석결과를 토대로 정책적 시사점을 제시하였다.

• 한국컴퓨터정보학회논문지
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• 제23권9호
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• pp.157-162
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• 2018

The purpose of this study is to investigate the relationship between resilience, burnout, and self-efficacy of Public Social Workers and to draw implications for effective management of Public Social Workers. In order to accomplish this study purpose, the resilience of Public Social Workers as an independent variable, burnout as a dependent variable of occupational identity, and self-efficacy as a moderating variable were selected. The causal relationship between resilience and burnout and the moderating effect of self-efficacy were analyzed. As a result of the analysis, the resilience of Public Social Workers showed a significant effect on burnout, and the effect of resilience on burnout varied according to self-efficacy, so there was a moderating effect of self-efficacy. Based on the results of this analysis, the theoretical implications and policy implications of this study are suggested.

• 한국컴퓨터정보학회논문지
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• 제22권9호
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• pp.179-185
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• 2017

The purpose of this study was to investigate the relationships between occupational identity, occupation satisfaction, and self-efficacy of care workers in care hospitals and to draw implications for improving the quality of care services. In order to accomplish the purpose of this study, the occupational identity of care workers in care hospitals as an independent variable, occupation satisfaction as a dependent variable of occupational identity, and self-efficacy as a moderating variable were selected. The causal relationships between occupational identity and occupation satisfaction and the moderating effect of self-efficacy were analyzed. As a result of the analysis, the occupational identity of care workers in care hospitals showed significant effects on occupation satisfaction and job commitment. Also, the effects of occupational identity on occupation satisfaction and job commitment differ depending on self-efficacy, which means that moderating effects of self-efficacy are significant. Based on this analytical results, the theoretical and policy implications of this study were suggested, and the directions and limitations of this study were suggested.

• 한국컴퓨터정보학회논문지
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• 제23권7호
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• pp.113-120
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• 2018

The purpose of this study is to derive implications for effective management of the public social worker by investigating the relationship between social support, job attitude and self-esteem of the public social worker. In order to accomplish this research purpose, social support of the public social worker as independent variables, job attitude as a dependent variable, and self-esteem as a parameter were analyzed and the relationship between these variables was analyzed. As a result, emotional, evaluative, material, and informational support, which constitute the social support of the public social worker, have a significant effect on job attitude, and self-esteem has a mediating effect on the relationship between these variables. Based on the results of this analysis, the importance of social support of the public social worker was suggested.

• 한국컴퓨터정보학회논문지
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• 제20권7호
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• pp.109-114
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• 2015

With the realization that the police's personal information gathering activities can violate the authority to decide one's information guaranteed by the Constitution, many people are interested in the legal terms of the police's information gathering and handling. The police's personal information gathering activities imply both the purpose of public welfare and order and the risk of violation of basic rights of citizens, their effective balance is critical. In this respect, this study reviews the principle of proportion as a principle of control of personal information gathering and handling (police intelligence activities) by state to discuss its implications on legislation.