• International Journal of Industrial Entomology and Biomaterials
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• 제33권1호
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• pp.15-23
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• 2016

One major constraint in the development and implementation of a successful and cost-effective area-wide integrated pest management (AW-IPM) programme with a SIT component for Bactrocera oleae (Diptera: Tephritidae) is the ability to produce a large number of high quality mass-reared individuals. The aim of this study was to develop a more efficient and practical egg collection system in an attempt to improve the mass-rearing of this species. The following basic parameters were examined: egg production per female, egg hatch, pupal recovery, pupal weight, adult emergence and percentage of fliers. Three different strains (Israel wild-type, France wild-type, and Greece laboratory) were tested and each strain was evaluated for six generations. Female flies of the Israel strain produced significantly more eggs per female than the other two strains, but egg hatch was significantly lower. Egg hatch of the France wild type and the Greece laboratory strain was similar. For all other parameters, there was no significant difference between strains; however, there was a significant generational effect for all parameters observed. As a result of this study, a protocol was developed for the mass-rearing of this species that included the use of large adult holding cages that could house up to 96,000 flies per cage. The newly developed method of egg collection using a flat wax panel as one of the sides of an adult holding cage proved to be cost-effective, efficient, making colony growth easier for industrial mass-rearing.

• The Korean Journal of Physiology
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• 제5권1호
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• pp.43-49
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• 1971

In an attempt to better understand the effect of whole body X-irradiation on the spontaneous motility of the isolated mouse duodenum and to clarify the possible radioprotective notion of reduced glutathione (GSH), a whole body X-irradiation of 1,000r was given to albino mouse either singularly or immediately after injecting GSH intraperitoneally to mouse in the dose of 1mg per gm of body weight. The total length of contraction of the isolated duodenum was recorded on kymograph every five minutes for 60 minutes, and the comparison was made with the control (i.e., normal). The results thus obtained are summarized as follows: 1. The spontaneous motility of the isolated duodenum in the X·irradiated groups showed a significantly elevated pattern for the first 15 minutes comparing with the control. The motility, however, decreased after 15 minutes and remained so in the X-irradiated groups to the level of the non-irradiated control, but 12 hours post-irradiation group showed a significantly increased motility throughout the experiment comparing with the control. 2. When GSH was injected intraperitoneally Prior to the whole body X·irradiation with 1,000r, the spontaneous motility of the isolated duodenum of mouse showed a significantly decreased pattern for the first 10 and 15 minutes comparing with the X·irradiated group followed by the similar motility thereafter comparing with the control and X-irradiated groups. 3. The above results suggest that GSH is effective as a radioprotector in terms of the motility of the isolated mouse duodenum.

• Asian-Australasian Journal of Animal Sciences
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• 제18권11호
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• pp.1557-1563
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• 2005

Ovarian follicular fluid contains both stimulatory and inhibitory agents that influence the growth and maturation of oocyte. In the present study, an attempt was made to isolate and study the biological properties of ovarian follicular fluid peptide(s) in buffaloes. Bubaline ovarian follicular was made steroid- and cell-free. A protein fraction was obtained by saturation (30-35% level) of the follicular fluid with ammonium sulfate. The protein fraction was purified with Sephadex-G 50 gel filtration chromatography and a single peak was obtained in the eluant volume, which was lyophilized. SDS-PAGE of the lyophilized fraction revealed a single band and the molecular weight of the peptide was 26.6 kDa. The peptide stimulated the cumulus cell expansion and in vitro maturation rate of oocytes in buffaloes in a dose dependent manner when it was incorporated at different dose levels (0, 10, 25, 50, 100 and 1,000 ng $ml^{-1}$ of maturation medium). The basic culture medium consisted of TCM 199 with Bovine serum albumin (0.3%). The in vitro maturation rates were comparable to those obtained with a positive control medium (TCM 199+20 ng EGF $ml^{-1}$+steer serum (20%)). Further purification and biological assays may throw more light on the nature and functions of this peptide.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.223-229
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• 2006

In this study, we have conducted kinetics and modelling studies of Centella asiatica cell growth and substrate uptake, in an attempt to evaluate cell growth for a better understanding and control of the process. In our bioreactor cultivation experiment, we observed a growth rate of 0.18/day, a value only 20% higher than was seen in the shake flask cultivation trial. However, the observed maximum cell dry weight in the shake flask, 10.5g/L, was 14% higher than was achieved in the bioreactor. Ninety seven percentage confidence was achieved via the fitting of three unstructured growth models; the Monod, Logistic, and Gompertz equations, to the cell growth data. The Monod equation adequately described cell growth in both cultures. The specific growth rate, however, was not effectively predicted with the Logistic and Gompertz equations, which resulted in deviations of up to 73 and 393%, respectively. These deviations in the Logistic and Gompertz models may be attributable to the fact that these models were developed for substrate-independent growth and fungi growth, respectively.

• Clinical and Experimental Pediatrics
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• 제48권9호
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• pp.953-959
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• 2005

목 적 : 극소 저출생 체중아에서 경구 수유가 불가능한 기간 동안 총정맥영양을 시행할 목적으로 경피 중심정맥 도관(percutaneous central venous catheter, PCVC)을 시술하는데 이의 가장 흔한 합병증인 패혈증은 신생아 이환율과 사망률을 높일 수 있다. 본 연구는 1,500 g 미만의 극소 저출생 체중아에서 시행한 PCVC의 유용성 및 도관과 관련된 합병증 발생을 알아보고자 하였다. 방 법 : 1998년 1월부터 2003년 12월까지 신생아 집중치료실에 입원한 1,500 g 미만의 저출생 체중아 중 PCVC를 시행했던 32명을 연구군(1,000 g 미만 17명, 1000-15,00 g 15명)으로 하였고, PCVC를 하지 않은 24명(1,000 g 미만 9명, 1,000-1,500 g 15명)을 대조군으로 하였다. 연구군과 대조군 간에 체중증가 양상과 2,000 g이 되는 시기를 비교하였고, 입원기간 동안 체중증가에 영향을 줄 수 있는 이환 질환 및 도관 관련 합병증을 조사하였다. 결 과 : 연구군과 대조군의 특징을 보면 출생체중, 재태주령, Apgar 점수, 호흡곤란 증후군, 인공환기 기간, 튜브영양을 처음 시작하는 시기, 경구수유 시작 시기 및 시작 시의 체중, 튜브영양에서 경구수유까지 걸린 기간, 총 정맥영양을 시작한 생후 일수와 기간, 종료시 체중 및 2,000 g에 도달하는 시기에 의미 있는 차이는 없었고, 총 정맥영양 기간만 연구군에서 $25.5{\pm}10.78$일로 대조군 $39.9{\pm}9.87$일에 비해 의의있게 길었다(P<0.001). 이환 질환 및 합병증에 있어서 호흡곤란 증후군, 만성 폐질환, 동맥관 개존, 뇌실내 출혈, 괴사성 장염, 패혈증, 골연화증 및 담즙 정체 발생빈도는 두 군간에 차이가 없었고 뇌실내 출혈만 연구군에서 87.5%로 대조군(58.3%)에 비해 의의있게 높았다. 연구군과 대조군을 1,000 g 미만과 1,000-1,500 g으로 나누어 비교해 본 결과, 연구군에서 대조군에 비해 1,000 g 미만에서는 패혈증 빈도가 의의있게 높았으며(35.3% vs 0%, P<0.05), 1,000-1,500 g에서는 뇌실내 출혈 빈도가 의의있게 높았고( 86.7% vs 40%, P<0.05), 2,000 g에 도달하는 시기도 오히려 늦었다. 결 론 : 경피 정맥도관술을 실시한 연구군에서 총 정맥영양기간이 길었지만 체중 증가에는 효과가 없었고 오히려 1,000 g 미만에서는 패혈증 빈도가 그리고 1,000-1,500 g에서는 뇌실내 출혈의 빈도가 높았기에 극소 저출생 체중아에서 경피 정맥 도관을 통한 총 정맥영양요법 시에는 무균적 조작과 수액 공급 속도 등에 더 주의를 기울여야 될 것으로 사료되었다.

• 대한수의학회지
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• 제39권4호
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• pp.786-796
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• 1999

As an attempt to develop an effective control method against theileriosis, recombinant antigen protein was produced. Thirty-two kDa membrane protein(MP) gene of T sergenti was amplified through RT-PCR from extracted total RNA of T sergenti isolated in Chonbuk, Korea. The amplified 869 bp of Korean T sergenti membrane gene was cloned and the base sequences were analyzed. The amplified gene was cloned into E coli expression vector, pQE32 plasmid vector, and the vector was introduced into E coli strain M15 to produce the recombinant membrane protein. For the induction of T sergenti membrane protein(KTs-MP), the plasmid harboring E coli strain M15 were cultured in the presence of IPTG, and the recombinant protein were purified by $Ni^+$-NTA agarose. Then, to confirm the authenticity of the produced membrane protein, molecular weight of expressed recombinant KTs-MP was analyzed by SDS-PAGE and Western blotting. The molecular weight of expressed recombinant protein was 32 kDa as expected. The recombinant KTs-MP was successfully recognized by anti-His Tag antibody, antisera of T sergenti infected cattle and monoclonal antibody of T sergenti membrane protein. Therefore, we concluded that the authentic 32 kDa membrane protein of T sergenti was produced as immunologically recognizable form.

• 한국자동차공학회논문집
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• 제13권4호
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• pp.74-80
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• 2005

This paper presents a misfire monitoring method by using the weight factor. According to OBD II(On-Board Diagnostics) regulations of the CARB (California Air Resources Board), an ECU (Electronic Control Unit) should detect misfires which occur in the internal combustion engine. A misfire is 1311owe4 by post-oscillations for short duration. Sometimes, the amplitude of oscillations may be as high as misfire and can be falsely detected as another misfire. To prevent this, the software designers do not attempt to detect another misfire for this short duration, during which the post oscillations exist. Because of this, ECU does not detect all the misfires and hence, the unstable state of automobile cannot be detected. If this happens for a long time, automobile may get damaged. To solve these problems, this paper suggests a new algorithm to detect misfire by using weighting factor Weighting factor is a concept to distinguish the misfire with the post oscillation and to improve the detection rate. This value of weighting factor is used for counting the misfire. This paper also shows the result of experiment done on a automobile using this software. The software is implemented using ASCET-SD which is preferred in the design of engine control. This paper's result show the possibility of improving the misfire detection by implementing this algorithm.

• 한국식품영양학회지
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• 제9권4호
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• pp.424-429
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• 1996

전분의 첨가가 냉동면의 조직감과 관능적 특성에 미치는 영향을 밝히기 위하여 감자초산변성전분, 옥수수초산변성전분, 찰옥수수전분, 옥수수전분, 감자전분, 밀전분 등 6종의 전분을 함량별로 첨가하여 복합분을 제조하였다. 이들의 아밀로그래프, 제면시의 조리시험, 냉동, 해동후 면의 최대절단강도를 측정하고 관능평가하였다. 감자초산변성전분과 감자전분을 첨가한 복합분의 호화개시온도는 대조구인 소맥분 100%보다 다소 낮아졌다. 최고점수는 초산변성전분류, 찰옥수수, 감자전분을 첨가한 경우 다소 높아졌다. 조리시의 체적과 중량은 다소 증가하고 고형분 용출량은 변성전분과 찰옥수수전분을 참가한 경우 다소 감소하였다. 냉동, 해동 조리 후 면의 최대절단강도는 감자초산변성전분 15%와 20%, 옥수수초산변성전분 15%를 첨가한 경우에 대조구보다 커졌다. 감자전분을 제외한 나머지 5종의 전분을 첨가시에 냉동면의 조직감과 색깔이 다소 향상되는 것으로 나타났다. 그 중에서 감자초산변성전분의 효과가 가장 높았다.

• The Korean Journal of Physiology
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• 제4권2호
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• pp.61-67
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• 1970

In view of the importance of sulfhydryl groups in producing a chemical protection against ionizing radiation, an attempt was made to evaluate the changes of intrinsic non-protein sulfhydryl (NP-SH) and non-Protein disulfide(NP-SS) of the lung and liver tissues of rabbits following the whole body X-irradiation with 900 r either in single or fractionated $(300\;r{\times}3)$ dose. NP-SH was measured by Ellman's method, and NP-SS was measured by the electrolytic reduction method described by Dohan. Experiment was performed at 1,3,5,24 and 48 hours post·irradiation, and the results were compared with the control. The results thus obtained are summarized as follows; 1) Intrinsic levels of NP-SH and NP-SS of normal rabbits were $0.77{\pm}0.10$ and $0.61{\pm}0.07\;{\mu}mol/gm$ wet weight in liver, and $0.28{\pm}0.03$ and $0.54{\pm}0.03$ in lung tissues respectively. 2) NP-SH of liver after single X-irradiation showed no significant change in general, but at 48 hours post·irradiation, it was elevated comparing with the normal value. 3) Levels of NP-SS in liver was decreased than the normal value in the irradiated groups, and the lowest level was observed at 3 hours after single X-irradiation and at 5 hours after fractionated irradiation. 4) In lung tissues, levels of NP-SS showed no significant change from the control at earlier experimental hours, but a great decrease was observed at later Part of the experiment.

• 한국식품조리과학회지
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• 제17권4호
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• pp.373-379
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• 2001

In an attempt to introduce paprika in wet noodles for improved organoleptic and other quality characteristics, the juices of orange paprika was added as a raw ingredient for wet noodle at 0(control), 15, 30, 45, and 60%(v/w). Overall, the cooked wet noodles containing 15 to 60% paprika juices showed higher sensory scores than control(noodle without Paprika juice), and the acceptabilities for color and overall preference of the products were significantly(p＜0.01) higher at the addition levels of 45% and 30 and 45%, respectively. The intensity analysis for the organoleptic quality characteristics of wet noodles with orange paprika juice showed that as the addition levels increased, there were significant(p＜0.001) increase in the values of acceptances for color and flavor of the products, but not in the tenderness and chewiness. Other quality characteristics like water absorption ratio, weight, volume, and turbidity of the broth after cooking of wet noodles increased in values as affected by the addition of paprika juices. In the Hunter's colorimetric characteristics of the products, the decreased lightness(“L”) along with the increased redness(“a”), yellowness(“b”), and ΔE were caused as the amounts of orange paprika juice increased in wet noodles. For toughness, no significant differences(p＜0.001) were noticed among the samples as measured by Waner-Bratzler blade attached to Texture Analyzer. The results of this study suggested that adding orange paprika juice to wet noodles, most preferably at 45%, increases the acceptance and organoleptic qualities of noodle by affecting the color and flavor of the final products.