• Reproductive and Developmental Biology
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• 제31권2호
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• pp.103-108
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• 2007

This study was carried out to investigate the effects of cryoprotectants, warming solution and removal of lipid on open pulled straw vitrification (OPS) method of porcine embryos produced by nuclear transfer (NT) of fetal fibroblasts. All solutions used during vitrification were prepared with holding medium consisting of 25 mM Hepes buffered TCM199 medium containing 20% fetal bovine serum (FBS) at $38.5^{\circ}C$. The blastocysts derived from NT with or without lipid were vitrified in each medium of different concentrations of dimethyl sulfoxide (DMSO) and ethylene glycol (EG). Also, blastocysts after cryopreservation were warmed into different concentrations of sucrose in warming solution. The optimal concentrations of cryoprotectants in vitrification solution were 10% DMSO + 10% EG in vitrification solution 1 (VS1) and 20% DMSO + 20% EG in vitrification solution 2 (VS2). The optimal concentrations of sucrose were 0.3 M sucrose in warming solution 1 (WS1) and 0.15 M sucrose in warming solution 2 (WS2). lipid removal from oocytes before NT enhanced the viability of NT embryos after vitrification. Our results show that use of the OPS method in conjunction with lipid removal provides effective cryopreservation of porcine nuclear transfer embryos.

• 대한간호학회지
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• 제47권4호
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• pp.456-466
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• 2017

Purpose: This study was designed to determine the effects of pre-warming on core body temperature (CBT) and hemodynamics from the induction of spinal anesthesia until 30 min postoperatively in surgical patients who undergo total hip replacement under spinal anesthesia. Our goal was to assess postoperative shivering and inflammatory response. Methods: Sixty-two surgical patients were recruited by informed notice. Data for this study were collected at a 1,300-bed university hospital in Incheon, South Korea from January 15 through November 15, 2013. Data on CBT, systemic blood pressure (SBP), and heart rate were measured from arrival in the pre-anesthesia room to 3 hours after the induction of spinal anesthesia. Shivering was measured for 30 minutes post-operatively. C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were measured pre-operatively, and 1 and 2 days postoperatively. The 62 patients were randomly allocated to an experimental group (EG), which underwent pre-warming for 30 minutes, or a control group (CG), which did not undergo pre-warming. Results: Analysis of CBT from induction of spinal anesthesia to 3 hours after induction revealed significant interaction between group and time (F=3.85, p=.008). In addition, the incidence of shivering in the EG was lower than that in the CG ($x^2=6.15$, p=.013). However, analyses of SBP, heart rate, CRP, and ESR did not reveal significant interaction between time and group. Conclusion: Pre-warming for 30 minutes is effective in increasing CBT 2 and 3 hours after induction of spinal anesthesia. In addition, pre-warming is effective in decreasing post-operative shivering.

• Ocean and Polar Research
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• 제25권4호
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• pp.503-518
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• 2003

This study examines the relationship among temperature, wind, and sea level pressure to understand recent warming in the vicinity of the Antarctic Peninsula. To do this, the surface air temperature, NCEP/NCAR reanalysis wind data and sea level pressure data for the period of 40 years are analyzed. The 40-year surface air temperature data in the Antarctic Peninsula reveals relatively the larger warming trends for autumn and winter than other seasons. The variability of the surface air temperature in this region is compared with that of the regional atmospheric circulation. The surface air temperature is positively correlated with frequency of northwesterlies and negatively correlated with frequency of southeasterlies. This relation is more evident in the northern tip of the Antarctic Peninsula for autumn and winter. The trend analysis of wind frequency in the study area shows increasing and decreasing trends in the frequency of northwesterlies and southeasterlies, respectively, in the northwestern part of the Weddell Sea for autumn and winter. And also it is found that these winds are closely related with decreasing of sea level pressure in the southeastern region of the Antarctic Peninsula. Furthermore from the seasonal variation of sea level pressure in this area, it may be presumed that decreasing of sea level pressure in the southeastern region of the Antarctic Peninsula is related with warming in the vicinity of the Antarctic Peninsula for autumn and winter. Therefore it can be explained that recent warming in the vicinity of the Antarctic Peninsula is caused by positive feedback mechanism, that is, the process that warming in the vicinity of the Antarctic Peninsula can lead to the decrease of sea level pressure in the southeastern region of the Antarctic Peninsula and these pressure decrease in turn lead to the variation of wind direction in northwestern part of Weddell Sea, again the variation of wind direction enhances the warming in the Antarctic Peninsula.

• 한국기후변화학회지
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• 제7권1호
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• pp.77-84
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• 2016

Seedling stage is particularly important for tree survival and is easily influenced by warming. Therefore, air temperature being increased due to climate change may affect physiological traits and growth of seedlings. This study was conducted to investigate the physiological and growth responses of Larix kaempferi seedlings to open-field experimental warming. 1-year-old and 2-year-old L. kaempferi seedlings were warmed with infrared lamps since April 2015 and April 2014, respectively. The seedlings in the warmed plots were warmed to maintain the air temperature to be $3^{\circ}C$ higher than that of the control plots. Physiological responses (stomatal conductance, transpiration rate, net photosynthetic rate and total chlorophyll content) and growth responses (root collar diameter (RCD), height and biomass) to experimental warming were measured. Physiological and growth responses varied with the seedling ages. For 2-year-old L. kaempferi seedlings, stomatal conductance, transpiration rate and net photosynthetic rate decreased following the warming treatment, whereas there were no changes for 1-year-old L. kaempferi seedlings. Meanwhile, total chlorophyll content was higher in warmed plots regardless of the seedling ages. Net photosynthetic rate linked with stomatal conductance also decreased due to the drought stress and decrease of photosynthetic efficiency. In response to warming, RCD, height and biomass did not show significant differences between the treatments. It seems that the growth responses were not affected as much as physiological responses were, since the physiological responses were not consistent, nor the warming treatment period was enough to have significant results. In addition, multifactorial experiments considering the impact of decreased soil moisture resulting from elevated temperatures is needed to explicate the impacts of a wide range of possible climate change scenarios.

• 한국주조공학회지
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• 제23권3호
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• pp.107-113
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• 2003

• 한국산림과학회지
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• 제103권2호
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• pp.203-210
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• 2014

본 연구는 지구 온난화와 관련한 대응 대책 마련을 위해서 대기 온도 상승에 따른 주요 침엽수의 종자 발아 및 발아 후 유묘의 생존율 변화를 알아보고자 수행하였다. 소나무와 전나무를 대상으로 적외선등을 이용하여 대조구 보다 온난화 처리구의 대기 온도를 $3^{\circ}C$ 높게 유지하는 실외 실험적 온난화 처리를 실시하였다. 온난화 처리에 따른 대기 온도 상승으로 두 수종 모두 발아율이 높아지는 경향을 보였지만, 전나무에서만 유의적 차이가 나타났다. 소나무와 전나무 두 수종 모두 온난화 처리구가 대조구보다 평균발아일수는 유의적으로 감소하였으며, 온난화 처리구에서 높은 발아속도와 발아세를 보였다. 온도 상승과 토양 수분 감소에 따라 전나무 유묘의 고사율은 증가하였지만, 소나무는 온난화 처리에 따른 차이를 보이지 않았다. 향후, 지구 온난화에 따른 대기와 토양 온도 상승 및 토양 수분감소로 양묘과정에서 종자 발아 및 유묘 생존율의 변화가 예상되기 때문에 지구 온난화에 대응할 수 있는 양묘기술 개선이 필요할 것으로 판단된다.

• Journal of Dental Anesthesia and Pain Medicine
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• 제15권4호
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• pp.193-200
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• 2015

Background: During head and neck surgery including orthognathic surgery, mild intraoperative hypothermia occurs frequently. Hypothermia is associated with postanesthetic shivering, which may increase the risk of other postoperative complications. To improve intraoperative thermoregulation, devices such as forced-air warming blankets can be applied. This study aimed to evaluate the effect of supplemental forced-air warming blankets in preventing postanesthetic shivering. Methods: This retrospective study included 113 patients who underwent orthognathic surgery between March and September 2015. According to the active warming method utilized during surgery, patients were divided into two groups: Group W (n = 55), circulating-water mattress; and Group F (n = 58), circulating-water mattress and forced-air warming blanket. Surgical notes and anesthesia and recovery room records were evaluated. Results: Initial axillary temperatures did not significantly differ between groups (Group $W=35.9{\pm}0.7^{\circ}C$, Group $F=35.8{\pm}0.6^{\circ}C$). However, at the end of surgery, the temperatures in Group W were significantly lower than those in Group F ($35.2{\pm}0.5^{\circ}C$ and $36.2{\pm}0.5^{\circ}C$, respectively, P = 0.04). The average body temperatures in Groups W and F were, respectively, $35.9{\pm}0.5^{\circ}C$ and $36.2{\pm}0.5^{\circ}C$ (P = 0.0001). In Group W, 24 patients (43.6%) experienced postanesthetic shivering, while in Group F, only 12 (20.7%) patients required treatment for postanesthetic shivering (P = 0.009, odds ratio = 0.333, 95% confidence interval: 0.147-0.772). Conclusions: Additional use of forced-air warming blankets in orthognathic surgery was superior in maintaining normothermia and reduced the incidence of postanesthetic shivering.

• 한국수자원학회논문집
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• 제32권6호
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• pp.617-625
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• 1999

지구 온난화는 산업혁명이후 이미 시작되었으며 최근에 와서 그 정도가 심해지고 있다. CO2와 같은 온실기체의 증가를 가장 큰 원인으로 하는 지구 온난화의 영향이 아직 정량적으로 밝혀지고 있지만 대기순환모형(General Circulation Model: GCM)을 이용한 연구에서 이러한 온실기체의 증가가 지구의 평균온도를 상승시킨다고 밝히고 있다. 지구 온난화는 전지구적 물의 순환에도 영향을 미쳐 지구 곳곳의 강수패턴에 변화를 가져오는데 근래에 자주 발생하는 대규모 가뭄이나 홍수화 같은 극치기상이 이러한 지구온난화의 간접증거로 자주 언급되곤 한다. 국내의 경우도 예외는 아니어서 근래에 들어 빈번한 홍수 및 가뭄을 경험하고 있다. 본 연구에서는 지구온난화에 따른 홍수 및 가뭄의 빈도변화 분석을 목적으로 한다. 이러한 연구목적을 달성하기 위해 먼저 현재상태가 지구온난화 과정의 한 시점이라는 가정 아래 현재까지 관측된 일강수량 자료를 분석하여 과거 연강수량의 증가가 일강수량의 분포에 어떤 식으로 영향을 미치고 있는지를 분석하여 보았다. 대상자료는 금강유역의 17개 지점을 선정하여 이용하였고, 분석결과로서 연강수량과 무강수일수는 상대적으로 작은 상관을 보이고 있음을 밝힐 수 있었으며 오히려 10mm이하 또는 50mm이상의 강수일수에 0.8-0.9의 높은 상관을 보임을 파악할 수 있었다.

• Clinical and Experimental Reproductive Medicine
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• 제47권4호
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• pp.312-318
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• 2020

Objective: The objective of the study was to compare the effects of long-term and short-term embryo culture to assess whether there is a correlation between culture duration and clinical outcomes. Methods: Embryos were divided into two study groups depending on whether their post-warming culture period was long-term (20-24 hours) or short-term (2-4 hours). Embryo morphology was analyzed with a time-lapse monitoring device to estimate the appropriate timing and parameters for evaluating embryos with high implantation potency in both groups. Propensity score matching was performed to adjust the confounding factors across groups. The grades of embryos and blastocoels, morphokinetic parameters, implantation rate, and ongoing pregnancy rate were compared. Results: No significant differences were observed in the implantation rate or ongoing pregnancy rate between the two groups (long-term culture group vs. short-term culture group: 56.3% vs. 67.9%, p=0.182; 47.3% vs. 53.6%, p=0.513). After warming, there were more expanded and hatching/hatched blastocysts in the long-term culture group than in the short-term culture group, but there was no significant between-group difference in embryo grade. Regarding pregnancy outcomes, the time to complete blastocyst re-expansion after warming is shorter in women who became pregnant than in those who did not in both culture groups (long-term: 2.19±0.63 vs. 4.11±0.81 hours, p=0.003; short-term: 1.17±0.29 vs. 1.94±0.76 hours, p=0.018, respectively). Conclusion: The outcomes of short-term culture and long-term culture were not significantly different in vitrified-warmed blastocyst transfer. Regardless of the post-warming culture time, the degree of blastocyst re-expansion 3-4 hours after warming is an important marker for embryo selection.

• 환경영향평가
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• 제17권1호
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• pp.67-79
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• 2008

Global warming, which is one of the most serious challenges, has been the subject of intense debate and concern for many scientists, policy-makers, and citizens for at least the past decade. To protect the health and economic well-being of current and future generations, we must reduce our emissions like carbon dioxide. Alternatives to achieve an energy future without serious global warming are to change to clean and renewable sources of energy like the wind, the sun lights, rivers, the biomass, hydrogen, and oceans. To identify some of the key and new environmental impacts associated with renewable energy and hydrogen energy, we set up the new conceptual methodology. Specifically, new identified environmental and health impacts are related with the usage of hydrogen energy. When comparing with fossil fuel, the renewable energies can reduce the release of carbon dioxide when they are used except hydrogen produced from fossil fuel. However, all renewable energy technologies are not appropriate to all applications or locations. Our results suggest that all of alternatives to replace fossil fuel can release the several global and local impacts although they seems to be smaller than the impacts from fossil fuel. Therefore, the quantitative and detail analysis to assess environmental impacts of the alternative energies might be useful to make our decision for the future energy against the global warming.