• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.235-241
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• 2002

Sophorolipid, a biosurfactant produced from Candida bombicola ATCC 22214, showed antimicrobial activity against Bacillus subtilis, Staphylococcus xylosus, Streptococcus mutans, and Propionibacterium acne at 4, 1, 1, 0.5 ppm, respectively. Also, 100 ppm of sophorolipid inhibited $50\%$ of cell growth of plant pathogenic fungus, Botrytis cineria. However, sophorolipid showed no effect on Escherichia coli, indicating that its selective antimicrobial activity depended on the cell wall structure. Treatment of B. subtilis with sophorolipid increased leakage of intracellular enzyme, malate dehydrogenase, indicating a possible interaction of sophorolipid with a cellular membrane. Comparing lactone-type and acid-type sophorolipids, the former showed a higher antimicrobial activity. Supplementing other surfactants showed no significant effects on the antimicrobial activity. Animal study showed that 5 g of sophorolipid per kg body weight by oral administration caused no toxicity, and sophorolipid induced no irritation on the skin. These results show potential use of sophorolipid as an active ingredient in healthcare products.

• Computers and Concrete
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• 제20권4호
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• pp.469-481
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• 2017

This paper aims to analytically investigate the effect of shear stress at the concrete-steel interface on the mechanical behavior of the circular steel tube-confined concrete (STCC) stub columns with active and passive confinement subjected to axial compression. Nonlinear 3D finite element models divided into the four groups, i.e. circumferential-grooved, talc-coated, lubricated, and normal groups, with active and passive confinement were developed. An innovative method was used to simulate the actively-confined specimens, and then, the results of the finite element models were compared with those of the experiments previously conducted by the authors. It was revealed that both the predicted peak compressive strength and stress-strain curves have good agreement with the corresponding values measured for the confined columns. Then, the mechanical properties of the active and passive specimens such as the concrete-steel interaction, longitudinal and hoop stresses of the steel tube, confining pressure applied to the concrete core, and compressive stress-strain curves were analyzed. Furthermore, a parametric study was performed to explore the effects of the concrete compressive strength, steel tube diameter-to-wall thickness ratio, and prestressing level on the compressive behavior of the STCC columns. The results indicate that reducing or removing the interfacial shear stress in the active and passive specimens leads to an increase in the hoop stress and confining pressure, while the longitudinal stress along the steel tube height experiences a decrease. Moreover, prestressing via the presented method is capable of improving the compressive behavior of STCC columns.

• 한국포장학회지
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• 제11권1호
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• pp.11-16
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• 2005

Fruits are subjected to complex dynamic stresses in the transportation environment. During a long journey form the production area to markets, there is always some degree of vibration present. Vibration inputs are transmitted from the vehicle through the packaging to the fruit. Inside, these cause sustained bouncing of fruits against each other and container wall. These steady state vibration input may cause serious fruit injury, and this damage is particularly severe whenever the fruit inside the package is free to bounce, and is vibrated at its resonance frequency. The determination of the resonant frequencies of the fruit may help the packaging designer to determine the proper packaging system providing adequate protection for the fruit, and to understand the complex interaction between the components of fruit when they relate to expected transportation vibration inputs. The first frequency of the pear in packaged freight be stacked in resonance frequency band of the pear packaged freight was increased from the bottom to the top of the stack but the second frequency of that in resonance frequency band of the pear was decreased. This indicated that the high damage score of the pear in bottom tier in vibration test was due to higher acceleration level in resonance frequency band of the pear.

• 한국가시화정보학회지
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• 제6권1호
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• pp.41-46
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• 2008

In this paper, the recirculation flow motion and mixing characteristics driven by air bubble stream in a rectangular water tank is studied. The time-resolved PIV technique is adopted for the quantitative visualization and analysis. 488 nm Ar-ion CW laser is used for illumination and orange fluorescent ($\lambda_{ex}=540nm,\;\lambda_{em}=560nm$) particle images are acquired by a PCO 10bit high-speed CCD camera (1280$\times$1024). To obtain clean particle images, 545 nm long pass optical filter and an image intensifier are employed and the flow rates of compressed air is 3 l/min at 0.5 MPa. The recirculation and mixing flow field is further investigated by time-resolved POD analysis technique. It is observed that the large scale recirculation resulting from the interaction between rising bubble stream and side wall is the most dominant flow structure and there are small scale vortex structures moving along with large scale recirculation flow. It is also verified that the sum of 20 modes of velocity field has about 67.4% of total turbulent energy.

• 대한기계학회논문집
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• 제11권2호
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• pp.331-344
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• 1987

본 연구에서는 직사각형 밀폐공간내에서 자연대류와 복사의 상호 영향에 대해 서 P-1 근사를 이용하여 수치적으로 해석하였다.밀폐공간내에서의 온도분포, 속도 분포 및 열전달계수를 구하였으며 열경계층내에서의 전도와 복사의 상호 영향에 대하 여 고찰하였다. 표면 복사만이 존재하는 경우에 대해서도 고찰함으로써 P-1 근사의 적용한계를 규명하였다. 벽면을 산광 방사 및 반사체(diffuse emitter and reflec- tor)로, 기체는 회색체(gray body)로 가정하였다. 이는 복사 물성치의 파장에 따른 변화를 고려할 때의 대단히 복잡한 계산 과정을 피할 수 있고, 현재의 이론적 수준에 비추어 복사 열전달의 열향을 정성적으로 규명하는데 타당한 가정이다.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2008년 영문 학술대회
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• pp.160-167
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• 2008

The effects of squealer rim height on three-dimensional flows and aerodynamic losses downstream of a high-turning turbine rotor blade have been investigated for a typical tip gap-to-chord ratio of h/c=2.0%. The squealer rim height-to-chord ratio is changed to be $h_{st}/c$=0.00(plane tip), 1.37, 2.75, 5.51, and 8.26%. Results show that as $h_{st}/c$ increases, the tip leakage vortex tends to be weakened and the interaction between the tip leakage vortex and the passage vortex becomes less severe. The squealer rim height plays an important role in the reduction of aerodynamic loss when $h_{st}/c{\leq}2.75%$. In the case of $h_{st}/c{\geq}5.51%$, higher squealer rim cannot provide an effective reduction in aerodynamic loss. The aerodynamic loss reduction by increasing $h_{st}/c$ is limited only to the near-tip region within a quarter of the span from the casing wall.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2012년도 제38회 춘계학술대회논문집
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• pp.304-308
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• 2012

본 연구는 에틸렌-공기 혼합물로 채워져 있는 변형 가능한 구리 관에서의 초음속 화염 전파를 수치적으로 살펴보았다. 탄화수소의 화염 전파를 해석하기 위하여 지배방정식으로 Navier-Stokes 방정식과 Arrhenius 형태의 1단계 화학 반응식을 활용하였으며 변형 가능한 관을 해석하기 위하여 Inviscid Euler 방정식을 활용하였다. 또한, 두 물질 간 경계면 추적을 위하여 Level-set 기법을, 경계값 결정을 위하여 ghost fluid 기법을 사용하였다. 이러한 수치적 기법을 바탕으로 관의 변화에 따른 초음속 화염 내 팽창파의 전파 및 그에 따른 간섭 현상을 밀도 및 속도 변화를 통해 확인하였으며 초음속 화염 전파에도 안전성이 확보되는 최소 관 두께를 예측할 수 있는 수치적 기반을 마련하였다.

• Structural Engineering and Mechanics
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• 제78권5호
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• pp.637-649
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• 2021

Great efforts have been conducted to investigate the seismic performances of the arch and rectangular underground structures, however, the differences between seismic responses of these two types of underground structures, especially the vault radian influencing the seismic responses of arch structures are not clarified. This paper presents a detailed numerical investigation on the seismic responses of arch underground structures with different vault radians, and aims to illustrate the rule that vault radian affects the seismic responses of underground structures. Five arch underground structures are built for nonlinear soil-structure interaction analysis. The internal forces of the structural components of the underground structures only under gravity are discussed detailedly, and an optimum vault radian for perfect load-carrying functionality of arch underground structures is suggested. Then the structures are analyzed under seven scaled ground motions, amounting to a total of 35 dynamic calculations. The numerical results show that the vault radian can have beneficial effects on the seismic response of the arch structure, compared to the rectangular underground structures, causing the central columns to suffer smaller axial force and horizontal deformation. The conclusions provide some directive suggestions for the seismic design of the arch underground structures.

• 한국추진공학회지
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• 제24권3호
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• pp.31-40
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• 2020

다수의 충격파에 대한 구조와 거동특성은 노즐 출구 마하수가 1.75인 모델에 대하여 유한체적기법을 사용하여 수치해석적으로 조사하였다. 먼저 이미지를 기반으로 한 충격파 진동특성을 Matlab 프로그램을 사용하여 분석한 후 특정 위치에서 벽면 정압변화를 분석하였다. 또한 다수 충격파들의 진폭 및 주파수도 조사하였다. 충격파 위치들 사이의 상호상관은 첫번째 충격파와 두번째 충격파는 서로 관련이 있는 반면에 다른 충격파들은 진동특성에서 위상 지연을 나타내었다. 벽면 압력변동의 RMS값은 충격파 위치에서 최대이며 유동의 다른 부분에서는 낮은 OS 표준편차값을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1284-1291
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• 2022

The rise of methicillin-resistant Staphylococcus aureus (MRSA) has resulted in significant morbidity and mortality, and clinical treatment of MRSA infections has become extremely difficult. Sortase A (SrtA), a virulence determinant that anchors numerous virulence-related proteins to the cell wall, is a prime druggable target against S. aureus infection due to its crucial role in the pathogenicity of S. aureus. Here, we demonstrate that isovitexin, an active ingredient derived from a variety of traditional Chinese medicines, can reversibly inhibit SrtA activity in vitrowith a low dose (IC₅₀=24.72 ㎍/ml). Fluorescence quenching and molecular simulations proved the interaction between isovitexin and SrtA. Subsequent point mutation experiments further confirmed that the critical amino acid positions for SrtA binding to isovitexin were Ala-92, Ile-182, and Trp-197. In addition, isovitexin treatment dramatically reduced S. aureus invasion of A549 cells. This study shows that treatment with isovitexin could alleviate pathological injury and prolong the life span of mice in an S. aureus pneumonia model. According to our research, isovitexin represents a promising lead molecule for the creation of anti-S. aureus medicines or adjuncts.