• Journal of the Korean Society of Food Science and Nutrition
• /
• v.18 no.2
• /
• pp.175-180
• /
• 1989

Toxins from Vibrio vulnificus cause Vibrio septicemia. Study was carried out for localization, characterization and toxicity of these toxins by injection thorough introspectional route to ICR(Insititude cancer research) mouse using Vibrio vulnificus M -1 isolated from patient and Vibrio vulnificus S-1 from sea water. No significant differences in lethal toxicity were observed between Vibrio vulnificus M-1 and Vibrio vulnificus $S-1.\;LD_{50}$ was $7.80{\times}10^6$ cells when these bacteria were injected to ICR mouse thorough intraperitoneal route. Crude hemolysin from Vibrio vulnificus S-1 did not show lethal toxiity and this lethal toxin were found to be endotoxin. This endotoxin were completely inactivated upon incubation at $80^{\circ}C$ for 20min.

• Journal of Life Science
• /
• v.7 no.2
• /
• pp.79-87
• /
• 1997

To determine the cause of Vibrio septicemia by understanding the characteristics endotoxin from Vibrio vulnificus, lethal dose, heat resistance and vascular permeability enhancing activity were svaluated using vegestative cell and cell homogenate and the result is as follows: 1. Vibrio vulnificus CDC B3547 of patient origin did not exihibit any significant difference in toxicity compared to Vibrio vulnificus B57 of enviroment origin. 2. Strong toxicity was observed when viable cell count of Vibrio vulnificus CDC B3547 was more than 10$^{7}$/ml. 3. Toxicity of cell homogenate was completely inactivited upon geating at 80$^{\circ}$C for 20min. 4. Cell homogenate did not show hemolyic activity but was acknowleged to have cytotoxicity. 5. Major lethal toxin against mouse was existed in Vibrio vulnificus CDC B3547; however, separation of LPS and LPS-protein complex was not successful using the current technique.