• Title/Summary/Keyword: vesicle

Search Result 738, Processing Time 0.024 seconds

NMR Studies of Lipid-Protein Interaction in Apolipoprotein B / Phosphatidylcholine Recombinants

  • Byong-Seok Choi;Cheal O Joe;Ke Won Kang
    • Bulletin of the Korean Chemical Society
    • /
    • v.11 no.3
    • /
    • pp.238-240
    • /
    • 1990
  • $^{31}P${$^1H$} nuclear Overhauser effects (NOEs) have been obtained for complexes formed between apolipoprotein B (apo B) and dipalmytoylphosphatidylcholine (DPPC) vesicles. NOE measurements have been conducted with broad-band irradiation of the entire $^1H$ spectrum in order to identify the proton source of the NOE. In a unilamellar vesicle formed spontaneously upon mixing aqueous suspensions of long-chain phospholipid with small amount of short-chain lecithin, the maximum NOE occurs at the N-methyl proton resonance position of the choline moiety. With addition of cholesterol to vesicles, the position of the NOE maximum shifts further away from the choline methyl frequency. For the ternary apo B-vesicle-cholesterol complex, the position of the maximum NOE lies halfway between those in vesicles with and without cholesterol.

A Permeability Measurement of Small Unilamellar Vesicles by 6-Carboxyfluorescein$^*$

  • Lee, Choong-Hee;Choi, Myung-Un
    • Bulletin of the Korean Chemical Society
    • /
    • v.5 no.4
    • /
    • pp.154-158
    • /
    • 1984
  • In order to characterize the permeability of small unilamellar vesicles (SUV), efflux of 6-carboxyfluorescein (6-CF) from the vesicles was monitored spectrophotofluorometrically. Since the entrapped highly quenched 6-CF (200 mM) became fluorescent upon release from the vesicles, the 6-CF could be used as an efflux probe. SUV containing entrapped 6-CF was prepared from egg phosphatidylcholine and separated by gel filtration on Sepharose 4B. Observed change of relative fluorescent intensity with time was sigmoidal. From this curve, the parameter of permeability was determined either by half-time or a released amount per unit time from the initial slope. Half-time of efflux of prepared SUV having 302 ng phospholipid/ml in 10 mM Tris-HCl buffer pH 7.4 was 21.0 min at $37{\circ}C$. Various factors which could affect the half-time were examined including temperature, pH, salt, and vesicle concentration. In particular the effect of vesicle concentration on the efflux revealed that the permeability can be a function of the concentration.

A Study on the Preparation of 5(6)-Carboxyflurescein-supported Phospholipid Liposomes and Evaluation of Bacterial Sensing Ability (5(6)-Carboxyflurescein을 담지한 인지질 리포좀의 제조 및 박테리아 센싱 능력 평가에 대한 연구)

  • Han, Minho;Jeon, Jaewoo;Lee, Junyoung;Shin, Eunsuk;Kim, Woojin;Kim, Samsoo
    • Textile Coloration and Finishing
    • /
    • v.33 no.4
    • /
    • pp.338-347
    • /
    • 2021
  • In the case of occlusive dressings currently used in dressings for burn treatment, it is impossible to confirm the replacement time, so replacement is delayed, resulting in additional infection. To solve this problem, liposomes capable of bacterial sensing were prepared using 5(6)-Carboxyfluorescein, Phosphatidylcholine, 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine, Cholesterol, and 10,12-Tricosadiynoic acid. In this study, evaluation of changes in drug encapsulation rate in liposomes according to changes in three types of phosphatidylcholine phospholipids during liposome production, high-performance phosphatidylcholine phospholipids selected through vesicle size analysis, low and high temperature stability evaluation, bacterial sensitization ability evaluation, animals cell responses were assessed.