• Korean Journal Plant Pathology
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• v.12 no.4
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• pp.395-401
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• 1996

국내의 고추, 사과, 포도, 스타티스(statice)에서 분리한 Glomerella cingulata 균주들의 병원성과 체세포화 합성군(vegetative compatibility group : VCG)과의 관계를 조사하였다. 사과, 포도, 스타티스에서 분리된 균주들은 전기주에 모드 병원성을 나타내었으나 고추에는 병원성이 없었다. 한편, 고추에서 분리된 균주들은 무상처접종시 병원성을 나타내지 못하였다. Nitrate nonutilizing(nit) mutant는 1.5%의 KClO\ulcorner을 첨가한 최소배지에서 선발하였다. nit mutnat들은 nit1, nit2, nit3, nitM의 4가지 표현형 중 하나에 속하였다. VCG는 표현형이 다른 nit mutan간의 대치배양을 통해 결정하였는데 사과, 포도, 스타티스에서 분리한 균주들간에는 같은 VCG를 나타낸 반면 고추에서 분리된 균주들은 다른 VCG로 나타났다. 따라서 G. cingulata 균주들의 체세포화합성은 병원성과 밀접하게 관련되어 있음을 알수 있었다. 또한 동일한 VCG에 속하며 기주가 다른 균주들의 대치배양시 heterokaryon을 형성했으나 자낭을 형성하지는 않았다.

• Korean Journal Plant Pathology
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• v.12 no.1
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• pp.33-40
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• 1996

우리나라 여러 딸기 재배지에서 전형적인 시들음 증상을 나타내는 이병식물에서 분리한 32개의 Fusarium oxysporum 균주들을 vegetative compatibility와 전기영동에 의한 동위효소상의 차이에 의해 분류하고 이들과 병원성과의 관계를 알아보았다. Nitrate nonutilizing(nit) mutant를 이용하여 vegetative compatibility group으로 분류해 본 결과 크게 A, B, C, D의 4개 그룹으로 나눌 수 있었으며 A그룹에는 15개 균주, B그룹에는 7개 균주, C와 D그룹에는 각각 2개 균주, 그리고 single VCG인 6개 균주가 존재하였다. 전기영동에 의한 esterase, catalase, acid phosphatase, leucin-aminopeptidase(LAP)의 동위효소상을 비교하여 본 결과 I, II, III, IV의 4개 그룹으로 나눌 수 있었으 I 그룹에는 18개 균주, II그룹에는 2개 균주, III그룹에는 6개 균주, IV그룹에는 6개 균주로 분류할 수 있었다. 또한, VCG의 A와 D그룹에 속하는 모든 균주들의 동위효소상의 I 그룹에 속하였으며 VCG B그룹의 7개 균주들 중에서 5개 균주가 동위효소상의 IV그룹, 7개 균주들 중에서 5개 균주가 동위효소상의 IV그룹, 나머지 2개 균주는 I 그룹과 III 그룹에 속하였으며 C그룹의 균주는 III과 IV 그룹에 속하였고, single VCGs들은 III 그룹에 4개 균주, II 그룹에 2개 균주가 속하므로 VCG와 동위효소상 간에는 밀접한 관계가 있음을 알 수 있었다. 4개 딸기 품종에 대해 병원성을 비교하여 본 결과 VCG A에서 선발된 2개 균주들은 보교조생에 대해 가장 병원성이 강하였으며 정보나 여홍에 대해서도 강한 병원성을 가지고 있었다. 반면에 B 그룹에서 선발된 2개 균주들은 4개 품종에 대해 병원성이 약하거나 거의 없었으며 C 그룹에서 선발된 1개 균주는 여홍에 대해 상대적으로 가장 강한 병원성을 가지고 있었다.

• The Plant Pathology Journal
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• v.27 no.2
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• pp.116-119
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• 2011

Pyrenophora graminea, the causal agent of leaf stripe disease, is an economically important pathogen of barley found worldwide. Forty-four isolates of diverse geographical origin within Syria were grouped into vegetative compatibility groups (VCGs) by demonstrating heterokaryosis by complementation tests using nitrate nonutilizing (nit) mutants. All isolates were grouped into three VCGs-1-A, 1-B and 1-C. No self-incompatibility was observed in any of the isolates tested. VCG 1-A was the most common group within growing regions in Syria and proved to be the most virulent of the VCGs identified. These data indicate that the level of virulence in P. graminea is related to VCG.

• Korean Journal Plant Pathology
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• v.11 no.4
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• pp.330-337
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• 1995

Forty-six isolates of Fusarium oxysporum collected from infected tomato plants and soils in greenhouses in Sedo, Chungnam and Angang, Kyeongbuk and 8 isolates of F. oxysporum f. sp. lycopersici from Japan and USA were used to determine vegetative compatibility groups (VCGs). Vegetative comaptibility was assessed on the basis of heterokaryon formation among nitrate nonutilizing mutants. All Korean isolates of F. oxysporum f. sp. lycopersici used in this study belonged to the same type of VCG (003) regardless of their geographic origin, cultivar and race, but they were incompatible with the foreign isolates of VCG 0030, 0031, 0032 and 0033. Based on the results, the Korean isolates of F. oxysporum f. sp. lycopersici were classified as a new VCG 003.

• Asian Journal of Turfgrass Science
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• v.25 no.2
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• pp.171-176
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• 2011

Sclerotinia homoeocarpa, the causal agent of dollar spot, is one of the most common pathogens of cool season turfgrasses in South Korea. The vegetative compatibility group (VCG) assay was carried out using nitrate-nonutilizing (nit) mutants recovered from 13 South Korean isolates with various geographical origins. The mutants were divided into four phenotypic classes based on mutation loci associated with nitrogen assimilation: nit1, nit2, nit3, and NitM. The recovered number of nit mutants greatly varied among the isolates, ranging from 0 to 15 mutants. Of the mutants isolated, nit1 and nit2 mutants were most common (80%) while NitM and nit3 were relatively rare. One dominant and four minor VCGs were determined from 18 mutant isolates tested. To study population structures of Korean S. homoeocarpa isolates and increase our understanding of its ecological and epidemiological aspects for dollar spot management on turfgrass, more generated mutants should be tested with more diverse isolate collections.

• The Plant Pathology Journal
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• v.21 no.3
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• pp.214-220
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• 2005

Vegetative compatibility groups (VCGs) of Fusarium oxysporum f. sp. radicis-lycopersici isolates collected from tomatoes in Korea were analyzed to determine the genetic characteristics and compared to those of foreign isolates. In comparison of VCG specificity with foreign VCG subgroup testers, Korean isolates were revealed to be VCG 0094 and to be similar to those of Israel and Florida, USA having a 'Universal' property. Results of this study will contribute the effective control of disease through precise estimation of fungal damage, the prediction of new pathogenic isolates appearance, and the movement of foreign pathogens.

• The Plant Pathology Journal
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• v.22 no.3
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• pp.222-229
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• 2006

Twenty-two isolates of Fusarium oxysporum f. sp. fragariae were obtained from diseased strawberry plants and their characteristics were investigated by vegetative compatibility group (VCG), random amplified polymorphic DNA (RAPD), and pathogenicity. Three major VCGs (A, B, and C) and one incompatible group were identified by nitrate reductase complementation test. The virulence pattern of the 22 isolates was studied in relation to four cultivars including Dochiodome, Red-pearl, Maehyang and Akihime. RAPD markers were used to determine genetic relationship, and created three major clusters among the 22 isolates of F. oxysporum f. sp. fragariae. Isolates belong to VCG-C were strongly pathogenic, and relatively high correlation was existed among VCG and RAPD, and virulence. In addition, VCG and RAPD pattern between pathogenic and non-pathogenic isolates were distinctly different.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2002.11a
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• pp.90.2-90
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• 2002

• The Korean Journal of Mycology
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• v.41 no.1
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• pp.14-20
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• 2013

We already reported four groups which contains some similar strains based on URP-PCR in the previous paper. The objective of this study was to confirm those strains by the amplified fragment length polymorphism (AFLP) and vegetative compatibility group (VCG). AFLP analysis showed no difference among these strains except ASI 2595 and 2183 in Weonhyeong group and ASI 2829 in Suhan group. They showed specific DNA bands only in the result of P + AG/M + AAG and P + GT/M + ATG primer combinations out of eight different combinations. The AFLP primers produced a total of 330 fragments between 80 and 1000 bp in length for 31 Pleurotus ostreatus strains. At a genetic similarity of 0.96, the UPGMA analysis separated the isolates into four distinct clusters. Each group was classified by similar strains. Confrontation test by vegetative compatibility groups (VCGs) also showed distinct line between strains from different groups, but no line between similar strains within the cluster. Our results indicate that most of similar strains was not distinctness. Thus, similar strains are considered to be very close on the genealogy of their parent or same strain with different name.

• The Plant Pathology Journal
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• v.15 no.1
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• pp.53-56
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• 1999

Fifty-three isolates of Fusarium graminearum were obtained from corn and barley samples in several provinces of Korea. Gas chromatography-mass spectrometric analysis of trichothecenes produced by these isolates revealed that 37 and 16 isolates were nivalenol (NIV)- and deoxynivalenol (DON)-chemotypes, respectively. Two hundred and seventy-five nitrate-nonutilizing (nit) mutants were obtained from the isolates. Of these mutants, 187 were identified as nit1, nit3, or NitM, but 88 could not be identified as one of these classes. The highest frequency of nit mutant was nit1 (65%), followed by nit3 (20%) and NitM (15%). Higher frequency of NitM was observed in DON-chemotypes than in NIV-chemotypes. The mutants were used for vegetative compatibility group (VCG) analysis by examining heterokaryosis using complementary mutant pairs. No heterokaryon formation was observed among all 1,248 pairwise combinations, suggesting that all isolates tested belong to different VCGs. Higher frequency of self-incompatibility was observed in NIV-chemotypes than in DON-chemotypes. These results suggest that the like-lihood of asexual genetic recombination may be very low I F. graminearum under the field condition.