• 한국소음진동공학회논문집
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• 제14권2호
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• pp.119-127
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• 2004

Butterfly valves are popularly used in service in the industrial and water works pipeline systems with large diameter because of its lightweight, simple structure and the rapidity of its manipulation. Sometimes cavitation can occur. resulting in noise, vibration and rapid deterioration of the valve trim, and do not allow further operation. Thus, the monitoring of cavitation is of economic interest and is very importance in industry. This paper proposes a condition monitoring scheme using statistical feature evaluation and support vector machine (SVM) to detect the cavitation conditions of butterfly valve which used as a flow control valve at the pumping stations. The stationary features of vibration signals are extracted from statistical moments. The SVMs are trained, and then classify normal and cavitation conditions of control valves. The SVMs with the reorganized feature vectors can distinguish the class of the untrained and untested data. The classification validity of this method is examined by various signals that are acquired from butterfly valves in the pumping stations and compared the classification success rate with those of self-organizing feature map neural network.

• 한국구조물진단유지관리공학회 논문집
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• 제12권2호
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• pp.181-192
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• 2008

본 논문은 콘크리트 슬래브교량의 반복적인 설계과정을 신속히 처리함과 동시에 모든 성과물을 작성함에 있어 웹을 기반으로 한 설계자동화 시스템 개발을 위한 것이다. 웹 기반 구조도면 처리기법은 웹기반 설계자 동화시스템 핵심 기술 중 하나이다. Active Server pages(ASP)는 설계 요소를 유용하게 활용하며 이를 데이터베이스에 저장하게 된다. eXtensible Markup Language(XML)과 XML기반 벡터그래픽은 구조 도면을 웹 화면으로 효과적으로 신속히 구현할 수 있다. 본 논문에서는 XML과 Scalable Vector Graphics(SVG)를 활용하여 웹 화면에 구조도면 및 설계 성과품을 작성하였다. 구조도면 작성 시 핵심 도면작성 요소기술로서 개발된 XML Data Island는 웹 기반 구조도면 작성 시 매우 효과적임을 증명하였다.

• The Plant Pathology Journal
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• 제25권2호
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• pp.179-183
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• 2009

To facilitate the genetic manipulation of Cladosporium phlei, a causal agent of leaf spot disease in timothy (Phleum pretense), protoplast-mediated transformation of C. phlei has been developed and the resulting transformants were characterized in this study. Hygromycin B resistance was applied as a dominant selection marker due to the sensitivity of C. phlei to this antibiotic. The transformation efficiency ranged from approximately 20-100 transformants per experiment. Southern blot analysis of stable transformants revealed that transformation occurred by way of stable integration of the vector DNA into the fungal chromosome. PCR analysis and plasmid rescuing of randomly selected transformants suggested that integration of tandem repeat copies of vector DNA was common. In addition, multiple integrations of the transforming vector at different chromosomal sites were also observed. The establishment of a transformation method for C. phlei facilitates strain improvement of this fungus and can be applied as an initial step in the molecular analysis of pigment production in this fungus.

• 미생물학회지
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• 제34권4호
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• pp.236-242
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• 1998

박테리오파지 P2-P4 시스템은 바이러스 조립과정 기작 연구를 위한 뛰어난 실험 소재로 연구되어 왔으나, 이 시스템에 유전자 조작상 필수적인 유용한 플라스미드가 없는 관계로 그의 필요성이 대두되고 있다. 본연구에서는 도움파지가 없을 때 플라스미드 상태로 존재하는 P4 ash8 (sid71)을 시작물질로, 항생제 내성 유전자를 도입하여 선택성을 줄 목적으로 P4 파지 증식에 불필요한 P4 DNA 단편을 pUC4-K 플라스미드의 kmr(kanamycin resistance) 유전자로 치환하여 P4 ash8(sid71) kmr을 생성하였다. 이 플라스미드는 박테리오파지 P2로 induction하여 박테리오파지 P4 상태로 증식시킬 수 있게 그 genome 크기를 조정하였으며, 파지 상태로 전환된 것을 burst size 결정 및 CsCl 부양 균등밀도 편차실험을 통해 입증하였다. 생성된 P4 플라스미드 유도체를 유전자 조작하여 P4의 integrase 기능을 잃은 변이체를 쉽게 만들 수 있었으며, 역시 박테리오파지 P4 상태로 증식시킬 수 있었다. 이러한 변이체 플라스미드의 안정성 실험을 수행하여, P4의 integrase 기능이 지속적인 플라스미드 상태 유지를 위해 필요하다는 것을 보여주었다.

• 한국전자파학회논문지
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• 제15권9호
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• pp.848-855
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• 2004

본 논문에서는 긴 코드 DS/CDMA DMB 하향링크 시스템에서의 신호공간에 기초한 새로운 채널추정기법을 제안한다. 신호공간과 잡음공간과의 직교성을 이용하는 종래의 부분공간 방식과는 달리 신호공간만을 이용하여 채널응답을 추정할 수 있다. 신호공간만을 이용하므로 제안된 기법은 종래의 부분공간 방식에서의 연산복잡도 문제를 해결할 수 있고 따라서 실제 구현 가능한 적절한 사이즈의 공분산 행렬로 부분공간 분석을 통한 채널추정기법에 사용될 수 있다. 논문을 통하여 제안된 기법과 관련된 수식을 도출하고 시뮬레이션 및 수치결과를 통해 제안된 기법의 유효성을 보인다.

• Journal of Microbiology and Biotechnology
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• 제31권12호
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• pp.1722-1731
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• 2021

The genus Streptomyces is intensively studied due to its excellent ability to produce secondary metabolites with diverse bioactivities. In particular, adequate precursors of secondary metabolites as well as sophisticated post modification systems make some high-yield industrial strains of Streptomyces the promising chassis for the heterologous production of natural products. However, lack of efficient genetic tools for the manipulation of industrial strains, especially the episomal vector independent tools suitable for large DNA fragment deletion, makes it difficult to remold the metabolic pathways and streamline the genomes in these strains. In this respect, we developed an efficient deletion system independent of the episomal vector for large DNA fragment deletion. Based on this system, four large segments of DNA, ranging in length from 10 kb to 200 kb, were knocked out successfully from three industrial Streptomyces strains without any marker left. Notably, compared to the classical deletion system used in Streptomyces, this deletion system takes about 25% less time in our cases. This work provides a very effective tool for further genetic engineering of the industrial Streptomyces.

• International Journal of Computer Science & Network Security
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• 제22권8호
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• pp.242-248
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• 2022

The article presents the analysis of modern methods of automatic comparison of original and unoriginal text to detect textual plagiarism. The study covers two types of plagiarism - literal, when plagiarists directly make exact copying of the text without changing anything, and intelligent, using more sophisticated techniques, which are harder to detect due to the text manipulation, like words and signs replacement. Standard techniques related to extrinsic detection are string-based, vector space and semantic-based. The first, most common and most successful target models for detecting literal plagiarism - N-gram and Vector Space are analyzed, and their advantages and disadvantages are evaluated. The most effective target models that allow detecting intelligent plagiarism, particularly identifying paraphrases by measuring the semantic similarity of short components of the text, are investigated. Models using neural network architecture and based on natural language sentence matching approaches such as Densely Interactive Inference Network (DIIN), Bilateral Multi-Perspective Matching (BiMPM) and Bidirectional Encoder Representations from Transformers (BERT) and its family of models are considered. The progress in improving plagiarism detection systems, techniques and related models is summarized. Relevant and urgent problems that remain unresolved in detecting intelligent plagiarism - effective recognition of unoriginal ideas and qualitatively paraphrased text - are outlined.

• 한국미생물·생명공학회지
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• 제19권3호
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• pp.215-221
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• 1991

YIp5를 사용하여 Cephalosporium acremonium ATCC 2033으로부터 Saccharomyces cerevisiae SHY3에서 발현되는 자율복제 기점 (ARS)를 분리하였다. 자율복제 기점을 갖는 40종의 vector 가운데 가장 안정성 (stability)이 높은 plasmid를 pCY-2라 명명하였으며 pCY-2는 C.acremonium에서 유래하는 3.7kb의 단편을 갖고 있었다. 또 Southern hybridization과 재형질전환을 통해 pCY-2가 효모내에서 자율적으로 복제되고 있다는 것을 확인하였다. 또한 pCY-2는 형질전환율과 안정성에 있어 효모의 ARS를 갖는 YRp7 vector보다 우수하였다.

• 한국측량학회:학술대회논문집
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• 한국측량학회 2003년도 추계학술발표회 논문집
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• pp.341-345
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• 2003

This study develops modeling processes for alignment optimization considering characteristics of intersections using genetic algorithms and GIS for road alignment optimization. Since existing highway alignment optimization models have neglected the characteristics of intersections, they have shown serious weaknesses for real applications. In this paper, intersection costs include earthwork, right-of-way, pavement, accident, delay and fuel consumption costs that are sensitive and dominating to alignments. Also, local optimization of intersections for saving good alignment alternatives is developed and embedded. A highway alignment is described by parametric representation in space and vector manipulation is used to find the coordinates of intersections and other interesting points. The developed intersection cost estimation model is sufficiently precise for estimating intersection costs and eventually enhancing the performance of highway alignment optimization models. Also, local optimization of intersections can be used for improving search flexibility, thus allowing more effective intersections. It also provides a basis for extending the alignment optimization from single highways to networks. The presented two artificial examples show that the total intersection costs are substantial and sensitive to highway alignments.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
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• pp.157-159
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• 2003

We have cloned and sequenced the cDNA coding fur a cellulase from the mulberry longicorn beetle, Apriona germari, with the polymerase chain reaction. And then we have constructed the recombinant plasmid vector for Bombyx mori transfomation experiment. (omitted)