• Journal of Embryo Transfer
• /
• v.33 no.4
• /
• pp.245-254
• /
• 2018

Pigs are considered as optimal donor animal for the successful xenotransplantation. To increase the possibility of clinical application, genetic modification to increase compatibility with human is an important and essential process. Genetic modification technique has been developed and improved to produce genetically modified pigs rapidly. CRISPR/Cas9 system is widely used in various fields including the production of transgenic animals and also can be enable multiple gene modifications. In this study, we developed new gene targeting vector and enrichment system for the rapid and efficient selection of genetically modified cells. We conducted co-transfection with two targeting vectors for simultaneous inactivation of two genes and enrichment of the genetically modified cells using MACS. After this efficient enrichment, genotypic analysis of each colony showed that colonies which have genetic modifications on both genes were confirmed with high efficiency. Somatic cell nuclear transfer was conducted with established donor cells and genetically modified pigs were successfully produced. Genotypic and phenotypic analysis of generated pigs showed identical genotypes with donor cells and no surface expression of ${\alpha}$-Gal and HD antigens. Furthermore, functional analysis using pooled human serum revealed dramatically reduction of human natural antibody (IgG and IgM) binding level and natural antibody-mediated cytotoxicity. In conclusion, the constructed vector and enrichment system using MACS used in this study is efficient and useful to generate genetically modified donor cells with multiple genetic alterations and lead to an efficient production of genetically modified pigs.

• Journal of Broadcast Engineering
• /
• v.26 no.2
• /
• pp.175-183
• /
• 2021

The study of the fine grained classification of images continues to develop, but the study of object recognition for animals with polymorphic properties is proceeding slowly. Using only pet images corresponding to dogs and cats, this paper aims to compare methods using image processing and methods using deep learning among methods of classifying species of animals, which are fine grained classifications. In this paper, Grab-cut algorithm is used for object segmentation by method using image processing, and method using Fisher Vector for image encoding is proposed. Other methods used deep learning, which has achieved good results in various fields through machine learning, and among them, Convolutional Neural Network (CNN), which showed outstanding performance in image recognition, and Tensorflow, an open-source-based deep learning framework provided by Google. For each method proposed, 37 kinds of pet images, a total of 7,390 pages, were tested to verify and compare their effects.

• The Journal of the Convergence on Culture Technology
• /
• v.9 no.5
• /
• pp.843-847
• /
• 2023

In this paper, we propose a model for detecting efficient fire identification to prevent fires that can lead to various industrial accidents, farmland and large forest fires, with the widespread use of various chemicals and flammable substances as modern technology advances. This paper presents an algorithm that can detect fire smoke in a high-efficiency and short time using images, and an algorithm based on SVM(Support Vector Machine) and K fold cross-verification technologies. By analyzing images, fire and smoke detection algorithms have relatively superior detection performance compared to existing algorithms, and the analysis of fire and smoke characteristics detected in this paper is analyzed stably and efficiently and is expected to be used in various fields that may be exposed to fire risks in the future.

• Asia-Pacific Journal of Business
• /
• v.14 no.4
• /
• pp.305-318
• /
• 2023

Purpose - This study analyzed the decoupling phenomenon between energy consumption and economic growth in Korea from 1990 to 2021. The main purpose of this study is to suggest policy implications for achieving a low-carbon society and decoupling that Korea must move forward in the face of the climate change crisis. Design/methodology/approach - This study investigated the relationship between energy consumption and economic growth by energy source and sector using the energy-EKC (EEKC) hypothesis which included the energy consumption on the traditional Environmental Kuznets Curve (EKC), and the impulse response function (IRF) model based on Bayesian vector auto-regression (BVAR). Findings - During the analysis period, the trend of decoupling of energy consumption and economic growth in Korea is confirmed starting from 1996. However, the decoupling tendency appeared differently depending on the differences in energy consumption by sources and fields. The results of the IRF model using data on energy consumption by source showed that the impact of GDP and renewable energy consumption resulted in an increase in energy consumption of bio and waste, but a decrease in energy consumption by sources, and the impact of trade dependence was found to increase the consumption of petroleum products. Research implications or Originality - According to the main results, efficient distribution by existing energy source is required through expansion of development of not only renewable energy but also alternative energy. Additionally, in order to increase the effectiveness of existing energy policies to achieve carbon neutrality, more detailed strategies by source and sector of energy consumption are needed.

• Korean journal of applied entomology
• /
• v.7
• /
• pp.5-13
• /
• 1969

Present system of seed potato production in Korea has several weak points and consequently has difficulties in covering annual shortage of 60,000 tons of seed potatoes. The author has an opinion that this so called 'High land system' of seed potato production adopted by the Government should be replaced by the 'Coastal area system' which is proposed by the author and has many advantages over present 'High land system'(2). In coastal areas where enormous acreage of rice paddies are spread, mostly around the villages. the primary host plants of the vectors are found. Therefore, the only source of aphid vectors are limited to the villages. The farmer's houses scattered more sparsely also have minor importance. In the previous paper(2), the author reported that the aphid vector populations were lower in the coastalareas than at Taegwanryong where the Alpine Experiment Station for the production. of seed potatoes is located. However, the number to vectors at Okku showed rather high density, where the trap was placed at the distance of 200 m from a village where peach and Hibiscus trees, the primary hosts of Myzus pesrsicae and Aphis gossypii were grown. To clarify the flight distance from the source of the aphid vectors, a trial was carried out in the Kwanghwal area, Kimje-gun, Cholla-pukto. on the western coast. 13 traps were placed at four directions and the distances between the traps were 250 m. (Fig. I) The traps 'Were operated from June 21 to October 31. The results are shown in Table 1. A total of some 70 species of aphids were found, including 5 speceis of potato virus vectors. The vectors are as follows: I. Myzus persicae (Sulzer) 2. Aphis gossypii Glover 3. Aulacorthum solani (Kaltenbach) 4. Lipaphis erysimi (Kaltenbach) 5. Macrosiphoniella sanborni (Gillette) Out of a total of 12,797 aphids, 5,187$(48\%)$ vectors were found. The trap catches at the 13 locations are shown in Fig. 2 and the numbers of the vectors at each location for each vector, except Macro-siphoniella sanborni. of which only a single individual was caught, are shown in Fig. 3-6. Number of vectors at C (3,279) (Centre of the village) is considerably higher than that at Suwon (763); however, EI. SI. WI and NI. where the distanecs from Care 250 m, showed lower numbers of vectors than that at Taegwanryong (347). The number of vectors at NI was rather than at the other 3 locations at the distance of 250m from the village. This was because C was in the southern part of the village. Consequently NI was much closer to the village than the other 3 locations of the same distance from C. Numbers of catches of the most important vector. Myzus persicae, are shown in Fig.3. The distribution pattern is typical except $S-2\;and\;W_3$, where several farmer's houses were found. If only the rice paddies were found in these locations. the numbers of the vectors would be small as the distances increase. Numbers of catches of the other 3 vectors are shown in Fig. 4-6. From these results. the author has drawn the following conclusions: 1. The aphid vector sources at the rice paddy belt in the western coast are the villages. 2. The vector densities at the locations where the distances are 250 m from the centre of the village are lower than that at Taegwanryong. 3. The vector densities become gradually lower as the distances from the centre of village increase. However. depending on the host plant situation at each location, the vector densities are variable. These minor sources of aphid vectors may be eliminated so that seed potatoes can be grown. 4. Thus. under the direction of specialists, fields suitable for seed potato production can be found in the coastal areas.

• Publications of The Korean Astronomical Society
• /
• v.15 no.spc2
• /
• pp.57-64
• /
• 2000

It is realized that the extraterrestrial matter is in ionized state, plasma, so the matter of this kind behaves as not expected because of its sensitiveness to electric and magnetic fields and its ability to carry electric currents. This kind of subtle change can be observed by an instrument for the magnetic field measurement, the magnetometer usually mounted on the rocket and the satellite, and based on the ground observatory. The magnetometer is a useful instrument for the spacecraft attitude control and the Earth's magnetic field measurements for the scientific purpose. In this paper, we present the preliminary design and the test results of the two onboard magnetometers of KARl's (Korea Aerospace Research Institute) sounding rocket, KSR­III, which will be launched during the period of 2001-02. The KSR-III magnetometers consist of the fluxgate magnetometer, MAG/AIM (Attitude Information Magnetometer) for acquiring the rocket flight attitude information, and of the search-coil magnetometer, MAG/SIM (Scientific Investigation Magnetometer) for the observation of the Earth's magnetic field fluctuations. With the MAG/AIM, the 3-axis attitude information can be acquired by the comparison of the resulting dc magnetic vector fields with the IGRF (International Geomagnetic Reference Field). The Earth's magnetic field fluctuations ranging from 10 to 1,000 Hz can also be observed with the MAG/SIM measurement.

• Journal of the Korean Institute of Telematics and Electronics C
• /
• v.36C no.4
• /
• pp.87-94
• /
• 1999

This paper presents a study on the off-line handwritten Hangul (Korean) character recognition using the partially connected neural network (PCNN), which is based on partial connections between the input receptive fields and the hidden nodes. The hidden nodes of three PCNNs have ten receptive fields and different input feature sets. And we introduce modular partially connected neural network (MPCNN), The MPCNN combines three PCNNs with a merging network. The learning scheme of the proposed networks is composed of two steps: PCNN learning step and the merging step of combining three PCNN s. In the merging step, another merging PCNN network is introduced and trained by regarding the hidden output of each PCNN as a new input feature vector. The performance of the proposed classifier is verified on the recognition of 18 off-line handwritten Hangul characters widely used in business cards in Korea.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.13 no.4
• /
• pp.283-292
• /
• 2020

Artificial intelligence, which is one of the representative images of the 4th industrial revolution, has been highly recognized since 2016. This paper analyzed domestic paper trends for 'Artificial Intelligence', 'Machine Learning', and 'Deep Learning' among the domestic papers provided by the Korea Academic Education and Information Service. There are approximately 10,000 searched papers, and word count analysis, topic modeling and semantic network is used to analyze paper's trends. As a result of analyzing the extracted papers, compared to 2015, in 2016, it increased 600% in the field of artificial intelligence, 176% in machine learning, and 316% in the field of deep learning. In machine learning, a support vector machine model has been studied, and in deep learning, convolutional neural networks using TensorFlow are widely used in deep learning. This paper can provide help in setting future research directions in the fields of 'artificial intelligence', 'machine learning', and 'deep learning'.

• Proceedings of the Korean Society of Propulsion Engineers Conference
• /
• 2008.03a
• /
• pp.790-798
• /
• 2008

Counter-rotating axial flow fan(CRF) consists of two counter-rotating rotors without stator blades. CRF shows the complex flow characteristics of the three-dimensional, viscous, and unsteady flow fields. For the understanding of the entire core flow in CRF, it is necessary to investigate the three-dimensional unsteady flow field between the rotors. This information is also essential to improve the aerodynamic characteristics and to reduce the aerodynamic noise level and vibration characteristics of the CRF. In this paper, experimental study on the three-dimensional unsteady flow of the CRF is performed at the design point(operating point). Flow fields in the CRF are measured at the cross-sectional planes of the upstream and downstream of each rotor using the $45^{\circ}$ inclined hot-wire. The phase-locked averaged hot-wire technique utilizes the inclined hot-wire, which rotates successively with 120 degree increments about its own axis. Three-dimensional unsteady flow characteristics such as tip vortex, secondary flow and tip leakage flow in the CRF are shown in the form of the axial, radial and tangential velocity vector plot and velocity contour. The phase-locked averaged velocity profiles of the CRF are analyzed by means of the stationary unsteady measurement technique. At the mean radius of the front rotor inlet and the outlet, the phase-locked averaged velocity profiles show more the periodical flow characteristics than those of the hub region. At the tip region of the CRF, the axial velocity is decreased due to the boundary layer effect of the fan casing and the tip vortex flow. The radial and the tangential velocity profiles show the most unstable and unsteady flow characteristics compared with other position of rotors. But, the phase-locked averaged velocity profiles of the downstream of the rear rotor show the aperiodic flow pattern due to the mixture of the front rotor wake period and the rear rotor rotational period.

• Research in Plant Disease
• /
• v.19 no.4
• /
• pp.273-280
• /
• 2013

Tomato spotted wilt virus (TSWV) was occurred at 8 areas including Naju, Suncheon, Younggwang, Youngam, and Shinan in Jeonnam province and the crops of Younggwang were severely damaged by TSWV. The hot pepper (Capsicum annuum), bell pepper (Capsicum annuum v ar. angulosum) and tomato (Solanum lycopersicon) in greenhouse and hot pepper in open field were infected by TSWV. Especially, hot pepper was severely damaged by TSWV infection. The survey data indicated that 1.1-30% in the nursery field at Naju, Suncheon, and Jangheung were infected by TSWV. Plants were infected by TSWV from early June to August. However, TSWV-infected seedlings from nursery fields showed the disease symptoms from May after transplanting. In pepper greenhouses, Frankliniella occidentalis was more dominant insect vector than Frankliniella intonsa. But in open field, the population of insect vector was opposed to greenhouse. In addition, the removal of weeds was able to delay the incidence of TSWV via side-window of greenhouse in Winter. Taken together, the control of weed and insect vector nearby side-window of greenhouse is important to prevent TSWV infection of plants.