• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2491-2494
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• 2014

Objective: To explore the mechanism and significance of tumor angiogenesis by observing changes of microvessel density (MVD) and expression of vascular endothelial growth factor (VEGF) in residual tumor tissues after cryoablation. Materials and Methods: A total of 18 nude mice xenograft models with transplanted lung adenocarcinoma cell line A549 were established and randomly divided into 3 groups when the maximum diameter of tumor reached 1 cm: control, cisplatin (DDP) and cryoablation. The nude mice were sacrificed after 21-d cryoablation to obtain the tumor tissues. Then immunohistochemistry was applied to determine MVD and the expression of VEGF in tumor tissues. Results: The tumor volumes of control group, DDP group and cryoablation group were $1.48{\pm}0.14cm^3$, $1.03{\pm}0.12cm^3$ and $0.99{\pm}0.06cm^3$ respectively and the differences were significant (P<0.01), whereas MVD values were $21.1{\pm}0.86$, $24.7{\pm}0.72$ and $29.2{\pm}0.96$ (P<0.01) and the positive expression rates of VEGF were $36.2{\pm}1.72%$, $39.0{\pm}1.79%$ and $50.8{\pm}2.14%$ (P<0.01), respectively, showing that MVD was proportional to the positive expression of VEGF (r=0.928, P<0.01). Conclusions: Cryoablation can effectively inhibit tumor growth, but tumor angiogenesis significantly increases in residual tumors, with high expression of VEGF playing an important role in the residual tumor angiogenesis.

• Journal of the Korean Wood Science and Technology
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• 제15권3호
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• pp.14-23
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• 1987

This study was carried out to investigate the anatomical characteristics, the radial and axial variation of the cell dimension and bulk density for four bamboo species used as the major material of bamboo products in Korea; Phyllostachys(P.bambusoides, P.edulis, P.nigra var.henonis, and Pnigra). The results obtained were summarized as follows; 1) In the outer part of culm, the mophological difference of cell diameter and distribution were not acknowledged. However, gum-like substance was occurred In the epidermis, hypodermis, cortex and metaxylem vessel of P.nigra, while it was occured only in the metaxylem vessel of P.edulis. 2) The protoxylem vessel in the central zone of culm-wall could be in the two types; the first type consisted of 4-5 small cell pieces occurred in P.edulis and P.nigra var. henonis, the second type of 1-2 large cell pieces in P.bambusoides and P.nigra. And transition of cell diameter from the vascular bundle sheath to the ground tissue was gradually enlarged in P.nigra and P.edulis but abruptly in P.bambusoides and P.nigra var. henonis. 3) Thin-wall tyloses were well developed in the outer and central zone of culm-wall in four species. 4) Compared with the central part of culm-wall, the number of parenchyma cells and the amount of the vascular bundle sheaths per unit area were a few in the inner part. The inner part nearest to the pith cavity was consisted of thick-well parenchyma cell. 5) The dimensional variations of metaxylem vessel and parenchyma cell in the radial direction were decreased from the inner part to the epidermis in all species observed. 6) The fiber length was the maximum in the central zone and its diameter was increased from the inner part to the outer part. In the axial variation. fiber length was slightly increased from the base and then decresed slowly toward the top, and its diameter was reverse. 7) The axial variation of the bulk density was continuously increased from the base toward the top and its radial variation was rapidly increased from the inner part to the epidermis.

• Journal of Chest Surgery
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• 제17권3호
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• pp.339-345
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• 1984

Thirty four patients with a ventricular septal defect, aged 2 months to 24 years [median 2.33 years], had lung biopsies during open heart procedures to assess the degree of pulmonary vascular disease. The patients were divided into two groups according to their age; group 1 - under 2 years of age [15 patients], and group 2- over 2 years of age [19 patients]. The, pathologic change of the pulmonary arterioles was correlated with pre-operative hemodynamic data in 34 patients. There was no significant correlation between the severity of pulmonary vascular disease according to Heath-Edwards classification and the patients age [X2=1.8381, P=0.1751 ]. There was a significant correlation between degree of medial wall thickness and arteriolar diameter and the level of preoperative peak pulmonary artery pressure and peak systemic artery pressure [Pp/Ps]. Also, there was a good correlation between the preoperative pulmonary vascular resistance and systemic vascular resistance [Rp/Rs]. There was no significant correlation between wall thickness and Pp/Ps, and Rp/Rs below the age of 2 years. We conclude that, in patients over 2 years of age, there was a significant correlation between the medial wall thickness of the pulmonary arteriole and elevation of Pp/Ps and Rp/Rs. This is not true in patients under 2 years of age.

• Journal of Chest Surgery
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• 제28권6호
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• pp.557-564
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• 1995

Microvel knitted double velour vascular grafts coated with biodegradable algin were evaluated in the canine experimental model as a new biologically coated Dacron graft. Three series of implantations were conducted involving the insertion of 6 mm diameter grafts in the abdominal aortae of mongrel dogs. The first series used the regular Microvel vascular grafts coated with algin,whereas the second and third series used Hemashield [collagen-coated grafts and the regular Microvel grafts with preclotting,respectively as control groups. Each series involved the implantation of one prosthesis for each of 2 preselected periods,namely 3 months and 6 months. In addition,algin-impregnated grafts were implanted for 4 hours,72 hours,2 weeks,and 4 weeks. All grafts were patent when the animals were sacrificed at intervals ranging from 4 hours to 6 months. Histological examinations revealed no obvious or significant differences in the healing characteristics of the algin-coated grafts and the control grafts after 3 months and 6 months of implantation. Endothelial cell-like cells were present on the midsegments of all grafts explanted from animals sacrificed after 3 months and 6 months,except a suspicious finding in the 3 month-implantation animal of a preclotted graft. With special stains,the algin became invisible between the polyester filaments during the first 3 months of implantation. This study has demonstrated that the use of a biodegradable algin coating is a feasible approach as biological sealants for textile arterial prostheses.

• 한국음향학회지
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• 제22권4호
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• pp.329-336
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• 2003

본 연구에서는 혈관 내부 조영용으로 사용될 수 있는 원형 배열 초음파 트랜스듀서를 설계하고 제작하였다. 트랜스듀서는 알루미나 튜브 위에 부착된 32개의 압전 소자와 전면 절연층, 고분자 후면층으로 구성된다. 우선 트랜스듀서의 방사특성 해석을 통해 원주방향으로 무지향성을 가지는 원형 배열 구조를 설계하였다. 압전소자로는 2-2형 압전복합재료를 채택하였다. 설계 결과에 따라 직경 3㎜의 원형 배열 트랜스듀서를 제작하고 그 음향특성을 측정하였으며, 측정 결과는 설계치와 잘 일치하였다. 실험적으로 확인된 무지향성은 본트랜스듀서의 IVUS (Intra-Vascular Ultra-Sound)센서로의 적용가능성을 확인시켜 주는 것이었다.

• Journal of Chest Surgery
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• 제26권2호
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• pp.80-85
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• 1993

Synthetic and biosynthetic vascular grafts of small diameter have long been considered to be prone to thrombosis, ultimately leading to the complete graft occlusion. Endothelial cell seeding onto synthetic blood-contacting surfaces has been suggested to be an ideal means to solve this problem. This study described a culture method of bovine endothelial cells and evaluated blood-compatibility and seeding efficiency of cultured endothelial cells. Bovine pulmonary artery endothelial cells were harvested enzymatically and grown to confluence on polystyrene culture flask surfaces using established techniques. The identification of endothelial cells was made through the demonstration of expression of factor VIII R:Ag by immunofluorescent technique. To quantitate the effect of improvement in blood-compatibility of viable endothelial cells, endothelial monolayers were exposed to blood containing $\^$111/In-oxine labeled platelets. Viable endothelial monolayers retained less labeled platelets than control surfaces. The Indium-labeled endothelial cells were seeded onto three different blood-contacting surfaces of Dacron vascular graft immobilized in specially equipped wells and incubated for specific time intervals (t=15, 30, 60, 120 minutes). Longer incubation times showed improved cell adherence in collagen-coated and fibrin-coated Dacron vascular graft groups. However in untreated Dacron grafts, no direct relationship was observed between incubation time and endothelial cell seeding efficiency. This may be due to leakage of endothelial cells through porosity of Dacron grafts in this in-vitro experimental condition.

• 한국임상수의학회지
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• 제18권1호
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• pp.78-81
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• 2001

A 12-year old female Pointer was referred to the Veterinary Teaching Hospital, College of Veterinary Medicine, Kyungpook National University for evaluation of emaciation. The owner had noted a dysuria and dyschesia 2 months before admitting to the hospital. The patient showed anorexia, depression and mild anemia. Radiographic findings indicated the mass below the urinary bladder, which pushed the intestine up to the spine. The patient was necropsied and grossly, two large masses were found on the heart and cranial thorax. Congestive nodules of 0.5-1.5 cm in diameter were scattered on both sides of the lungs. The left ventricular wall was hypertrophied and adult heartworms were found in right atrium. A large mass, filled with blood, 9-10 cm in diameter, originated from the rectum, compressed urethra. The gall bladder was filled with bile juice and the spleen was mild atrophied. The cortex of the kidney and the intestine were congested. On microscopic observation, there were neoplastic cells forming irregular vascular channels in the lung, kidney and heart. The vascular channels contain variable amount of blood. In the lung, the cells were almost elongated and the nuclei of neoplastic cells were round or ovoid and hyperchromatized. Based on the observation, the case was diagnosed as a hemangiosarcoma.

• Journal of Trauma and Injury
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• 제18권2호
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• pp.172-174
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• 2005

Trauma of the vascular structure is not poplular event. In obstructive atherosclerotic vascular disease, we sometimes have needed bypass surgery. The long length subcutaneous prosthetic vascular graft are vulnerable to injury. But prosthetic vessel rupture after trauma has been rare report. A 68-year-old man was referred to Department of Emergency of the Gyeongsang National University Hospital. After he had had a blunt trauma, he found a newly appearing pulsating mass of 10 cm diameter on his right chest wall. The lesion had a turbulent blood flow in the cavity of the mass by ultrasonographic finding. The lesion was a rupture of superficial prosthetic vascular graft under the skin.

• Archives of Plastic Surgery
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• 제36권5호
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• pp.597-604
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• 2009

Purpose: Compound tissue defects remain a challenge to reconstructive surgeons. The objective of this study was to introduce examples of successful reconstruction of compound defects of the head and neck and upper and lower limbs, using chimeric flaps based on the subscapular vascular system. Methods: We report 19 reconstruction cases using chimeric flaps based on the subscapular vascular system. The scapular flap, scapular fascia, scapular bone, parascapular flap, latissimus dorsi, latissimus dorsi perforator flap, latissimus dorsi myocutaneous perforator flap, serratus anterior, serratus anterior fascia, and rib bone were used as components for chimeric flaps. 12 cases had defects of the upper limb, three in the lower limb, three in the head and neck area, and one case had a defect of the thoracoabdominal wall. Results: Defect sizes ranged from $6{\times}8cm$ to $20{\times}22cm$. The component used most often for skin coverage was the latissimus dorsi perforator flap; for soft tissue bulk, the latissimus dorsi; for fascia coverage, the serratus anterior fascia flap; and for bone reconstruction, the scapular bone flap respectively. All cases were successfully reconstructed without additional operative procedures or flap necrosis. Conclusion: Because it is fairly easy to employ vascular pedicles of sufficient length and diameter, enabling the use of diverse types of tissue with various shapes and sizes, the use of chimeric flaps based on the subscapular vascular system allows one - stage reconstruction tailored to the characteristics of the defect area.

• Archives of Plastic Surgery
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• 제50권2호
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• pp.182-187
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• 2023

We aim to provide our surgical techniques, and outcomes of functional scrotal reduction procedures with complete preservation of the genitourinary original anatomy in a simple way without using complicated skin grafting or skin advancement flaps in Patients with huge and long-standing scrotal lymphedema 18 patients ages ranged from 14-65 with a median of 30 years. Functional scrotal and penoscrotal reduction was attained in all cases, without distortion of the genitourinary anatomy and without the need for advancement, rotational or free flaps, maximal scrotal diameter was reduced from median of 61[48-92] cms to a median of 25[21-29] cms (P<0.0001) and remained almost unchanged at the end of the follow up period 26[22-34] cms (P<0.0001). Sexual performance and voiding capacity were improved in all patients, testicular vascularity was unaffected and the Glasgow Benefit Inventory (GBI) for the quality of life showed marked enhancement in the total 55.5[50-72], general 55.5[50-72], social 100[50-100] and physical 16.6[16-33] points subscales. According to our experience, surgery remains the gold standard treatment for management of huge scrotal lymphedema, successful preservation of the genitourinary functions can be attained despite the size in most cases with excellent cosmoses.