• Journal of Microbiology and Biotechnology
• /
• v.16 no.12
• /
• pp.1984-1989
• /
• 2006

Deacetylated chitosan oligosaccharide (COS) had effective antiprotozoal activity against Trichomonas vaginalis (Minimal Inhibitory Concentration, MIC 0.25%), whereas 80% acetylated cas showed no antiprotozoal activity (MIC > 1 %). an the other hand, 80% acetylated cas showed growth stimulatory activity against the protozoa. When T. vaginalis was treated with 98% deacetylated COS at 0.25% concentration, the viability of the protozoa was rapidly decreased within 15 min, and the protozoa completely died within 40 min. Ultrastructural changes of trichomonads treated with COS included a loss of defined nuclear membrane and endoplasmic reticulum membranes, an increase in the number of free ribosome, vacuolation, and ultimately lysis of the cell membrane. These results indicate that deacetylated COS can be used as an antitrichomonal agent, although its lethal mechanism is not known.

• Korean Journal of Veterinary Research
• /
• v.35 no.4
• /
• pp.809-814
• /
• 1995

The acute and subacute toxicity of fumomsin $B_1$ was evaluted in fingerlings of common carp, Cyprinus carpio. Dipping of fish for acute toxicity was performed for a period of 48 hours, and the $TLm_{48h}$ value(median tolerance limit) was more than 1000 ppm in common carp. Severe damages were observed in various organs and among them, clubbing of gill lamella, lytic degeneration and vacuolation of liver cells, and epithelial edema of renal tubules were relatively prominent. The most significant changes were hyperbasophilic foci of liver cells in subacute toxicity test and these can imply the possibility of hepatocarcinogenecity of fumonisin $B_1$.

• Journal of Nutrition and Health
• /
• v.19 no.3
• /
• pp.161-168
• /
• 1986

In order to investigate the effect of riboflavin on cholesterol and MLHP (methyl linoleate hydroperoxide) -induced fatty liver electron microscopically, riboflavin was given to rate receiving high cholestero and MLHP diet for 4 weeks, 8 week old male Sprague-Dawley rats were administrated diets daily, as following group ; usual diet (control ), riboflavin 910 ug/20gm BW), cholesterol (50mg/gm BW), MLHP(0.1ml/20gm BW), cholesterol plus MLHP, cholesterol plus riboflavin, cholesterol plus MLHP plus riboflavin, by means of intubation into the stomach for 4 weeks. Each group consisted of 10 rats, The liver of the animals were examined ultrstructurally by transmission electronmicroscope. The results wee as follows ; 1) The three group including cholesterol, MLHP, cholesterol plus MLHP feeding resulted in fatty liver. 2) The main finding of the fatty liver were swelling and vacuolation of mitochondria, a lot of lipid droplets, disarrangements and loss of rough endoplamic reticulums. 3) the most striking features in the group of cholesterol plus riboflavin, cholesterol plus MLHP plus riboflavin feeding were decrease of size and number of lipid droplets, mitochondrial swelling and vesiculation, with restortion of rough endoplasmic retriculums. It is postulated with above findings that riboflavin was effective in prevention of cholesterol and MLHP -induced fatty liver.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.1
• /
• pp.167-170
• /
• 2008

A fungal strain BCP, which parasitizes Botrytis cinerea gray mold pathogen, was isolated and identified as Acremonium strictum. BCP strain overgrew the colonies of B. cinerea and caused severe lysis of the host hyphae. Frequent penetration and hyphal growth of A. strictum BCP inside the mycelia of B. cinerea were observed under light microscopy. In addition, some morphological abnormalities such as granulation and vacuolation of the cytoplasm were observed in mycelia and spores of B. cinerea. In dual culture test, A. strictum BCP strongly inhibited the mycelial growth of several plant pathogenic fungi as well as B. cinerea. To our knowledge, this is the first report on mycoparasitism of Acremonium species on B. cinerea.

• Journal of Veterinary Clinics
• /
• v.15 no.2
• /
• pp.291-302
• /
• 1998

Four out of 15 dogs were successfully induced diabetes mellitus with intravenous iqiection of 30 mg of streptozotgin and 50 mg of alloxan monohydrate per kilogram body weight and maintained more than 9 weeks without iqiection of insulin or oral hypoglycemic sgent Histopathologicallyi these four dogs have typical diabetic lesions such as degeneration and vacuolation of pancreatic islet cells, and fatty change of liver at necropsy in the end of study. Serum glucose level increased dramatically at 24 hours post-injection but serum fructosamine level increased gradually and reached plateau at 31-41 days post-injection of streptozotocin and alloxan. Serum fructosamine concert%lion correlated very well with serum glucose concentration of preceding 4-7 weeks in experimentally induced diabetic dogs. Our data suggest that serum fructosamine reflects mean glucose concentration of preceding 4-7 weeks in experimentally induced diabetic dogs.

• Proceedings of the Korean Society of Veterinary Pathology Conference
• /
• 2002.11a
• /
• pp.141-141
• /
• 2002

A patient (Painter+Viszula mixed breed, 4 months old, male), with chief historic sign of acute vomiting, hemorrhagic diarrhea, anorexia and dyspnea during 4 days was admitted ta College of Veterinary Medicine, Kyungpook National University. Necropsy findings were revealed ulcer and hemorrhage contained hemorrhagic diarrhea in gastrointestinal tract, severe emphysema and hemorrhage in the lung and kidney and cardiac hypertropy. Histopathological changes showed emphysema, hyperemia and hemorrhage in the lung, severe hyperemia, hemorrhage, hepatic vacuolation and cellular necrosis in the liver, hyperemia, hemorrhage, necrosis of tubular epithelium in the kidney, hemorrhage in cardiac muscle and hyperemia, necrosis and sloughing of epithelium in the intestine. In this case, we diagnosed as a paraquat poisoning.

• Korean Journal of Veterinary Research
• /
• v.34 no.2
• /
• pp.369-374
• /
• 1994

The acute and subacute toxicity of fungicide folpet was evaluated in fingerings of common carp, Cyprinus carpio and goldfish, Carrassius auratus. Dipping of fishes for acute toxicity was performed for a period of 24h, and the TLm value(median tolerance limit) was 1.52 ppm in common carp and 1.45 ppm in goldfish. Severe damages were observed in various organs and among them, clubbing of gill lamella, lytic degeneration and vacuolation of liver cells, and epithelial edema of renal tubules were relatively prominent. The most significant changes were hyperbasophilic foci of liver cells in subacute toxicity test and these can imply the possibility of hepatocarcinogenecity of folpet.

• The Korean Journal of Cytopathology
• /
• v.5 no.2
• /
• pp.160-166
• /
• 1994

Collecting duct carcinoma of the kidney is an unusual variety of renal carcinoma considered to arise from the epithelium of the collecting ducts. We recently experienced a case of fine needle aspiration cytology of collecting duct carcinoma of the kidney in a 17 year-old girl. The smear revealed many cellular clusters of ordinary papillary pattern, characterized by clumping of cells with nuclear overlapping, in a slightly necrotic background. The tumor cells had abundant delicate granular cytoplasm with some having vacuolation. The nuclei were only slightly pleomorphic with somewhat coarse chromatin and one or more small nucleoli Some nuclei showed irregular nuclear membrane and nuclear groove. A few polymorphs were also present.

• Journal of Plant Biology
• /
• v.38 no.1
• /
• pp.25-32
• /
• 1995

The distribution of RNA in the anther wall of Brassica napus during male gametogenesis was followed by 3H-uridine autoradiography. Silver grain(SG) density was not above background in the anther wall just after microspore was released from tetrad callose wall. Significant accumulation of SGs occurred in tapetum, endothecium, and epidermis before microspore vacuolation. Accumulation of RNA in the tapetal cells was peak before the vaculation occurred in the microspore. With the onset of tapetal senescence at the partially vacuolated microspore stage, SGs decreased and they completely disappeared in the tapetum at the bicelled pollen stage. Accumulation of RNA in the endothecial cells was peak after the microspore mitosis and continued just after the generative cell mitosis. Appreciable SGs also occurred in cells of epidermis from nonvacuolate microspore stage to bicelled pollen stage. During this period, SG density was almost same and was not high as compared with tapetum or endothecium. At tricelled mature pollen stage, no incorporation occurred in anther wall. SGs were found mostly over the nucleouls and chromation of the cell nuclei.

• Journal of Plant Biology
• /
• v.36 no.3
• /
• pp.241-249
• /
• 1993

The pattern of RNA synthesis during male gametogenesis of Brassica napus was studied using 3H-uridine autoradiography. No incorporation of isotope occurred in the newly released microspore and the nonvacuolate, furrowed microspore. Peak incorporation of label during male gametogenesis occurred in the uninucleate, furrowed microspores showing various degrees of vacuolation. In this microspore stage, silver grains were localized in the nucleus and cytoplasm. Moderate incorporation of the isotope occurred in the nulceus of the vacuolated microspore. After the microspore mitosis, isotope incorporation occurred predominantly in the nucleus of the vegetative cell with little or no incorporation in the generative cell. In tricellular pollen, no incorporation of isotope was observed in both the vegetative nucleus and the sperms. Silver grains almost completely disappeared from tricellular mature pollen grains ready to germinate.