• Analytical Science and Technology
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• v.13 no.3
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• pp.385-393
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• 2000

A gas chromatography-mass spectrometric (GC/MS) procedure for the determination of amineptine (dihydro-10, 11-dibenzo[a, d] cycloheptenyl-5-amino-7-heptanoic acid) and its main metabolites in human urine was described. Amineptine has been known to be extensively metabolized by the ${\beta}$-oxidation of the heptanoic side chain with formation of pentanoic side chain metabolite ($C_5$-metabolite), and lactamizarion by internal dehydration of (${\beta}$-oxidized metabolite (${\delta}$-lactam). The detection of these compounds was based on acid hydrolysis, liquid-liquid extraction and trimethylsilylated derivatization of the carboxylic acid group. For the determination of amineptine and its metabolites in biological fluids, selected ions at the m/ 192, molecular ion and one of the characteristic ions were monitored by GC/MS. On the excretion study of amineptine in human urine, 70-90% of amineptine, ${\delta}$-lactam, and $C_5$-metabolite were found to be excreted within 4 hours and their excretion completed within 20 hours.

• Journal of the East Asian Society of Dietary Life
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• v.15 no.5
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• pp.558-565
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• 2005

In this study, Schizandriae fructus which has been used in oriental medicine and folks remedy, was studied to apply to functional foods and oriental medicinal cuisine. The aim of this experiments was to investigate the effects of Schizandriae fructus water extract(SFE) on the renal function, plasma renin activity, plasma levels of aldosterone and arterial natriuretic peptide(ANP) in rats. Spargue-Dawley rats weigh 200g, were randomly assigned to 3 groups such as basal diet only(BDG), basal diet with $0.5{\mu}L/g$ SFE(LAG) and basal diet with $1.0{\mu}L/g$ SFE(HAG). The results were as follows. Water balance decreased significantly after administration for 2 weeks compared with the control period in HAG. Urine volume increased significantly after administration for 1 week compared with the control period in LAG and HAG. Urinary excretion of sodium increased significantly after administration for 1 week and for 2 weeks compared with the control period in LAG and HAG. Urinary excretion of creatinine increased significantly after administration for 2 weeks compared with the control period in HAG. Plama levels of ANL decreased significantly after administration of $SFE(0.5{\mu}L/g)$. Plasma levels of aldosterone decreased significantly after administration of $SFE(1.0{\mu}L/g)$. There results indicated that Schizandriae fructus can improve the renal function through increased urine volume and sodium excretion. These results imply that SFE could be used as a potent food resource for diet therapy or clinical nutrition.

• The Korean Journal of Pharmacology
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• v.11 no.2
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• pp.1-8
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• 1975

This study was carried out to observe the effect of testosterone on carbonic anhydrase inhibiting action of acetazolamide. Carbonic anhydrase activities in the kidneys of mice were measured by Philpot and Philpot method(1936) at 30, 90 and 150 minutes after intravenous administration of saline(0.5 ml/10 g) or acetazolamide (0.25 mg/10 g) in mice pretreated with testosterone (0.1 mg/10 g). The changes in volume and pH of urine as well as those in urinary electrolytes, such as $Na^+,\;K^+\;and\;Cl^-$ were measured at 15 minutes interval for 150 minutes in the rabbit pretreated with double administrations of testosterone(10 mg/kg), 1 hour and 18 hours, prior to the administration of acetazolamide (10 mg/kg). The results were as follows: 1. Carbonic anhydrase activities in the kidneys of mice of testosterone-pretreated groups were significantly higher than those of acetazolamide-treated group at 30 minutes. No significant changes of carbonic anhydrase activities were observed in testosterone-pretreated groups compared with saline-treated groups. 2. Combined administrations of acetazolamide and testosterone exhibited higher carbonic anhydrase activity than those group of acetazolamide alone in the kidney of mice through observed period of 150 minutes. 3. There were no significant changes in the excretion rate of urine and urinary electrolytes in the group of rabbits with testosterone administerone alone. Urine volume as well as $Na^+\;and\;Cl^-$ excretion rates in the combined treated group of acetazolamide and testosterone were significantly lower than that of acetazolamide group throughout experimental period except 15 minutes after drug administration at the time transient increase was shown. 4. Generally lower $K^+$ excretion rate was observed in the combined treated group of acetazolamide and testosterone compared with the single acetazolamide-treated group and the testosterone-pretreated group shows lowest excretion rate of potassium.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.4
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• pp.573-578
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• 1999

An experiment was conducted to investigate the effect of diet for growing-finishing pigs with high level of non-starch polysaccharides (NSP) from by-products on nitrogen excretion and nitrogen losses from slurry during storage. Sixteen commercial crossbred barrows of about 68 kg BW were randomly allotted to one of four diets. The control diet was formulated using tapioca and rice as basal energy sources. In the other diets, tapioca was replaced by either coconut expellar, rice bran or beer by-product. The diets differed mainly in the amount and compostition of NSP. After a 12-day adaptation period, urine and faeces were collected separately in metabolism cages for 9 days. Urine and faeces from the first four days were used to analyse the nitrogen partitioning. Urine and faeces from the last 5 days were mixed as slurry. The slurry was sampled at the end of the collection period and again after 30 days storage, to analyse for nitrogen to calculate the losses. Increasing dietary NSP reduced urinary nitrogen and nitrogen losses from the slurry during storage. The pigs fed the diet based on beer by-product excreted the most nitrogen via faeces and the least nitrogen via urine. Nitrogen losses from slurry of pigs fed the beer by-product were from 34 to 65% lower than from the other three diets. It is concluded that including NSP-rich by-products in the diet of growing-finishing pigs reduces urinary nitrogen excretion and nitrogen losses from slurry during storage.

• Biomolecules & Therapeutics
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• v.4 no.4
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• pp.349-353
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• 1996

The present study examined pharmacokinetic profiles of KBP31705-Cl27 and KBP30603-901, new platinum coordination complexes synthesized as anticancer candidates, in comparison with two well-known platinum-containing anticancer agents, cisplatin and carboplatin in rats. Under sodium pentobarbital anesthesia of male Sprague-Dawley rats, urinary bladder, and femoral artery and vein were catheterized for urine collection, blood sampling and drug injection, respectively Following i.v. administration of cisplatin (2 mg/kg), KBP31705-C127 (2 mg/kg), carboplatin (20 mg/kg) or KBP30603-901 (20 mg/kg), blood samples were collected at 2, 4, 6, 8, 10, 15, 20, 30, 45, 60 and 120 minutes. Urine samples were collected at 1-hr interval for 4 hr. Platinum concentrations in plasma and urine were measured using an inductively coupled plasmamass spectrometer. The plasma concentration-time curves were biphasic for all drugs during the time period studied. Compared with cisplatin, KBP31705-C127 showed similar decay patters in the alpha- and betaphases with slightly lower plasma concentrations. Urinary platinum excretion for cisplatin and KBP31705-C 127 was 56 and 52% of the administered dose in 4 hr, respectively. With regard to carboplatin and KBP 30603-901, a similar decay pattern was also observed in the alpha-phase. The half life of KBP30603-901 in the beta-phase, however, was much longer than that of carboplatin, which was consistent with the urinary excretion results that 46 and 59% of the administered dose were excreted in the urine in 4hr, respectively. The results suggest that platinum coordination complexes are primarily excreted via the renal route and KBP30603-901 can elicit longer duration of action due to slower renal excretion compared to carboplatin.

• Journal of the Korean Society of Food Science and Nutrition
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• v.4 no.1
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• pp.1-6
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• 1975

After ablactation, wistar strain white male rats, weighing 270g and 340g, were fed with a diet of CLEA for three months. The whole daily excretion of each feces and urine were collected, and extracted with water($80^{\circ}C$ hot water). The combined extraction were filtered and the $B_2$ was determined with the parts of the filterates by the lumiflavin fluorometric method, and the FMN, FAD and FR with the rest of the filterates by paper chromatography. The following results were obtained; 1. $B_2$ contents in the feces were $27.52{\gamma}$ per 100 grams per body weight, and $83.93{\gamma}$ per each rat per day. 2. $B_2$ contents in the urine were $18.47{\gamma}$ per 100 grams per body weight, and $56.33{\gamma}$ per each rat per day. The total daily excretion of $B_2$ contents in the feces were 1. 5 times as much as in the urine. 3. Among the total daily $B_2$ excretion of one white wistar strain rat in the feces were the following ; FAD, 81.0% ; FMN, 14.9% ; FR, 3.3%. Therefore the order of the contents were FAD>FMN>FR.

• Journal of Pharmacopuncture
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• v.22 no.1
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• pp.35-40
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• 2019

Objective: Adriamycin (ADR) is an important anti-cancer drug which can cause renal toxicity. Given the known anti-inflammatory and antioxidant effects of Plantago major (P. major), the aim of this study was to determine the effects of hydroalcoholic extract of P. major on ADR- induced nephropathy in rats. Methods: Fifty male Wistar albino rats were randomly divided into 5 groups including: control, ADR (5 mg/kg), ADR + P. major (600 and 1200 mg/kg) and P. major (1200 mg/kg). The animals were treated with P. major extract for 5 consecutive weeks and ADR was intravenously injected on the 7th day of the study. Urine and serum samples were collected on days 0, 14, 21, 28, and 35 for the measurement of serum cholesterol and albumin levels and urine protein excretion rate. At the end of the study, the left kidneys were removed for apoptosis assessment. Results: Administration of ADR significantly decreased serum albumin level and increased serum cholesterol and urine protein excretion rate as well as, apoptotic cell numbers compared to the control group (P < 0.001) while had no effect on glomerular filtration rate (P > 0.05). Treatment with P. major, in both 600 and 1200 mg/kg doses, increased serum albumin level and decreased serum cholesterol concentration, urine protein excretion rate and as well as the number of apoptotic cell compared to the ADR group (P < 0.001). Conclusion: Our results showed that the P. major extract effectively protects against ADR- induced nephropathy by reducing kidney apoptosis and improving renal functioning in rats.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.1 no.1
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• pp.18-22
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• 2001

Renal colic, hematuria, dysuria and stone passage were developed in younger brother (4 year 6 month old boy). But the elder sister (6 year old girl)had no specific symptoms and signs. The identification of the disease was proved by cyanide nitroprusside test and amino acid analysis of urine. In our patients the chromatographic amino acid patterns of urine showed remarkably increased excretion of cystine, ornithine, lysine, and arginine. They are managed by adequate hydration with Shohl solution for rendering the urine more alkaline, and alpha-mercaptopropionylglycine (Thiola).

• The Korean Journal of Physiology
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• v.9 no.2
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• pp.33-39
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• 1975

Changes of urinary aldosterone excretion, concurrent sodium and potassium excretion following furosemide administration were studied in normotensive young Korean with high sodium intake, moderate sodium restriction and marked sodium depletion. After intravenous injection of furosemd 40mg, plasma and urine samples were collected at every thirty minutes for two hours. Plasma-and urinary aldosterone, electrolyte concentration and urine flow rate were measured by means of radioimmunoassay or flamephotometry. Relations of urinary aldosterone to concurrent sodium or potassium/sodium ratio, and of urinary aldosterone to concurrent plasma aldosterone activity were studied. Following were the results: 1. Furosemide administration resulted in a increased urinary aldosterone concentration and unchanged or somewhat decreased sodium concentration in course of time after the injection. 2. Urinary potassium concentration showed initial decrease and subsequent increase in course of time after furosemide administration and it resulted in a gradual increase in urinary potassium/sodium ratio. 3. Studying the relations between urinary aldosterone excretion and potassium/sodium excretion ratio, or sodium excretion were meaningless because of the urinary flow rate after the injection was decreased with time course. 4. Furosemide administration showed a good relationship of urinary aldosterone concentration to concurrent potassium/sodium ratio rather than concurrent sodium concentration in subjects with sodium restriction, but no meaningful relationship was detected in subjects with high sodium intake because increasing rate of the ratio was not so wide. 5. Furosemide also resulted a reasonable relation of plasma aldosterone concentration to concurrent urinary aldosterone concentration especially during low sodium intake. 6. Above results suggested that relation of urinary aldosterone concentration to K/Na ratio following furosemide administration during sodium restriction is significant and has a benefit to reduce the variation induced by kalemic change showing in the diragram for daily aldosterone to sodium excretion.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.12 no.2
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• pp.53-66
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• 1999

This study has been carried out to investigate the effects of Dojuksan on the renal functions and internal secretion system, as water balance, urine volume, urinary excretion of sodium and potassium, free water clearance, urinary excretion of creatinine, plasma levels of artrial natriuretic peptide (ANP), plasma levels of aldosterone and plasma renin activity, comparing experimental group which Dojuksan water extract were administrated with control group. Sprague-Dawley rats, about 200-250 g, were used for this experiment. The results of this study were as follows: 1. Water balance decreased significantly after the administration of Dojuksan water extract. 2. Urine volume increased significantly after the administration of $100{\mu}l$ Dojuksan water extract per 100g rat. 3. Urinary excretion of sodium increased significantly but urinary excretion of potassium did not change after the administration of Dojuksan water extract. 4. Free water clearance decreased significantly after the administration of Dojuksan water extract 5. Urinary excretion of creatinine increased significantly after the administration of Dojuksan water extract 6. Plasma renin activity did not change after the administration of Dojuksan water extract 7. Plasma levels of artrial natriuretic peptide (ANP) did not change after the administration of Dojuksan water extract 8. Plasma levels of aldosterone decreased significantly after the administration of 200 ${\mu}Dojuksan water extract per l00g rat The results suggest that Dojuksan increase the urinary excretion of sodium. and thus reduce the water balance, which resulted from suppression of sodium reabsorption into renal tubule by increasing glomerular filtration rate and decreasing aldosterone.