• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.42-42
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• 2018

Two unrecognized species of Sparganium of Korea were found during the our field expeditions and phylogenetic analyses of specimens deposited in the Herbarium of Korea National Arboretum (KH). S. coreanum H. $L{\acute{e}}v$. was first reported as a new species based on the specimen (Taquet, 2150) collected from Jeju Island. It has been recognized as synonym or infraspecific taxa of S. stoloniferum and S. eurycarpum or even never recognized recently. However, phylogenetic tree showed that S. coreanum is monophyletic and has sister relationship with S. eurycarpum. Furthermore, additional distribution localities were also found by herbarium survey. Morphological characteristics and distribution information of S. coreanum will be discussed. Another Sparganium species found from Mt. Daeam is occurring either as floating or emergent. Although we could not identify this species since lack of any flowers or fruits for two year surveys, phylogenetic analyses results showed that this species belong to the clade of S. glomeratum, which is distributed in high elevation lakes and marshes of Europe, Asia, and North America. Additional survey of morphology and report will be needed.

• Animal Systematics, Evolution and Diversity
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• 제28권4호
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• pp.297-303
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• 2012

New Korean lumbricids are described: Eisenia gaga Blakemore sp. nov. from remote Gageodo Island and E. sindo Blakemore, sp. nov. from an island at Incheon. Both are comparable to Eisenia koreana (Zicsi, 1972) from near Pyongyang, North Korea. A remarkable yet previously unrecognized similarity to American earthworm Eisenoides carolinensis (Michaelsen, 1910) is discussed, but synonymy is rejected on tenuous morphological grounds (form of nephridial vesicle bladders) with taxonomy supported by objective molecular data (mtDNA cytochrome c oxidase subunit 1 barcodes).

• 식물분류학회지
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• 제49권3호
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• pp.203-208
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• 2019

Sparganium coreanum, a barely recognized species in Korea, was rediscovered during a field survey by the authors, who conducted a re-examination of specimens deposited in the Herbarium of the Korea National Arboretum (KH). This species was described initially by H. $L{\acute{e}}veill{\acute{e}}$ from a specimen collected by F. Taquet from Jeju-do (Taquet 2150). Subsequently, however, it was overlooked and unrecognized among South Korean flora. Several populations of S. coreanum were found in the southern part of the Korean Peninsula and on Jeju-do, although it has long been recognized as S. erectum owing to certain vegetative morphological characteristics shared between the two species, such as robust stems, a similar plant height, and globose rhizomes. However, it is distinct from S. erectum by the number of female heads on the lowest inflorescence branch and the size and shape of the fruit. In this study, we provide a detailed description, illustrations, and photographs with a revised taxonomic key for identification of Sparganium species in Korea.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 추계학술대회 및 정기총회
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• pp.41-41
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• 2015

Oomycetes belong to the kingdom Straminipila, a remarkably diverse group which includes brown algae and planktonic diatoms, although they have previously been classified under the kingdom Fungi. These organisms have evolved both saprophytic and pathogenic lifestyles, and more than 60% of the known species are pathogens on plants, the majority of which are classified into the order Peronosporales (includes downy mildews, Phytophthora, and Pythium). Recent phylogenetic investigations based on DNA sequences have revealed that the diversity of oomycetes has been largely underestimated. Although morphology is the most valuable criterion for their identification and diversity, morphological species identification is time-consuming and in some groups very difficult, especially for non-taxonomists. DNA barcoding is a fast and reliable tool for identification of species, enabling us to unravel the diversity and distribution of oomycetes. Accurate species determination of plant pathogens is a prerequisite for their control and quarantine, and further for assessing their potential threat to crops. The mitochondrial cox2 gene has been widely used for identification, taxonomy and phylogeny of various oomycete groups. However, recently the cox1 gene was proposed as a DNA barcode marker instead, together with ITS rDNA. To determine which out of cox1 or cox2 is best suited as universal oomycete barcode, we compared these two genes in terms of (1) PCR efficiency for 31 representative genera, as well as for historic herbarium specimens, and (2) in terms of sequence polymorphism, intra- and interspecific divergence. The primer sets for cox2 successfully amplified all oomycete genera tested, while cox1 failed to amplify three genera. In addition, cox2 exhibited higher PCR efficiency for historic herbarium specimens, providing easier access to barcoding type material. In addition, cox2 yielded higher species identification success, with higher interspecific and lower intraspecific divergences than cox1. Therefore, cox2 is suggested as a partner DNA barcode along with ITS rDNA instead of cox1. Including the two barcoding markers, ITS rDNA and cox2 mtDNA, the multi-locus phylogenetic analyses were performed to resolve two complex clades, Bremia lactucae (lettuce downy mildew) and Peronospora effuse (spinach downy mildew) at the species level and to infer evolutionary relationships within them. The approaches discriminated all currently accepted species and revealed several previously unrecognized lineages, which are specific to a host genus or species. The sequence polymorphisms were useful to develop a real-time quantitative PCR (qPCR) assay for detection of airborne inoculum of B. lactucae and P. effusa. Specificity tests revealed that the qPCR assay is specific for detection of each species. This assay is sensitive, enabling detection of very low levels of inoculum that may be present in the field. Early detection of the pathogen, coupled with knowledge of other factors that favor downy mildew outbreaks, may enable disease forecasting for judicious timing of fungicide applications.

• 한국동물위생학회지
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• 제23권4호
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• pp.341-348
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• 2000

Nowadays we continue to face challenges to the safety of our foods. It seems clear that contaminated meats provide the major route of listeria monocytogenes transmission from the environment to humans. L monocytogenes is the most important species associated with disease in humans among the listeria sp. The current incidence of symptomatic listeriosis caused by L monocytogenes is uncertain. Although the number of reports in the literature on listeriosis are increasing, it is likely that they are actually unrecognized or underreported because of a lack of awareness on the part of some laboratory workers who fail to isolate or identify these organisms. To get the information of sanitary development, we survey various meats (beef, pork, etc) in Kyongbuk area. Samples were collected from local meat shop at Kyongbuk area. Total sixty six case were isolated and identified from one hundreds and seven samples. L innocua was the most abundant in listeria sp. Among U isolates, the number of isolated L innocua was 43 (65.2%). The numbers of isolated L murrayi, L welshimeri, L monocytogenes and L seeligeri were 12(18.2%), 7(10.6%), 3(4.5%) and 1(1.5%), respectively, but L grayi, L iuanouii were not Isolated.

• 식물분류학회지
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• 제46권4호
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• pp.348-355
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• 2016

2015-2016년 사이 라오스 식물상을 조사하는 과정에서 발견한 미기록 현화식물을 보고한다. 라오스 푸카오쿠오아이 국가생물다양성보전지구에서 야목단과(Melastomataceae)의 Phyllagathis tuberosa (C. Hansen) Cellinese & S. S. Renner와 꼬리겨우살이과(Loranthaceae)의 Tolypanthus pustulatus Barlow를 발견하였으며, 이들은 태국의 기준표본 채집지 외에는 아직까지 채집된 바 없는 종들로, 라오스에 분포하는 것을 처음으로 확인하였다. 한편, 인동과(Caprifoliaceae)의 Lonicera bournei Hemsl.는 20세기초 라오스에 분포하는 것으로 보고된 바 있으나 이후 채집 기록이 없다가, 라오스 시앙쾅주 포사보국가보호지구에서 다시 발견하였다. 본 연구의 결과는 라오스 및 동남아시아 국가의 식물상 및 그 특성을 규명하는 데 도움이 될 것으로 기대된다.

• 한국전통조경학회지
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• 제38권4호
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• pp.1-11
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• 2020

녹산은 경복궁 내부에 위치한 구릉 형태의 녹지로서 문화재공간으로 주목받지 못한 장소이다. 이에 본 연구는 녹산이 성립된 배경과 변화 양상을 고찰하여 경관적 의의를 도출하고자 실록, 지도류, 사진에 대한 문헌 분석과 현장조사를 실시하였다. 연구 결과 녹산의 정체성은 풍수적 내맥(來脈), 소나무림, 사슴과 관련되며, 경관적 의의는 다음과 같다. 첫째, 「경복궁전도」 외 다수의 고지도에는 백악으로부터 경복궁 내부까지 이어지는 산줄기들이 묘사되었는데, 그중에서 녹산은 강녕전과 문소전으로 이어지는 내맥에 위치함으로써 형성된 숲이다. 북궐도상에서 녹산에는 육우정(六隅亭), 남여고(藍與庫), 녹직처소(鹿直處所), 그리고 남북 방향의 어구와 담장이 표현됨으로써 최소한의 시설과 숲으로 구성된 원형경관으로 이해할 수 있다. 둘째, 세종대에 경복궁의 북쪽 궁장이 건설되었으며, 경복궁 창건 시기에 연조 뒤쪽부터 신무문 안쪽을 후원으로 인식했던 반면에 경복궁 중건 시기는 신무문 밖에 새로운 후원 영역을 마련하였다. 이러한 배경에서 녹산만 내맥의 산줄기를 유지하였다. 그러나 일제강점기에 신무문 밖 후원에 대규모 관사가 건립되고 경무대 부지에 총독관저와 도로가 개설되면서 지형이 크게 변화되었다. 여기에 1967년 청와대와 녹산 사이의 청와대로가 신설되면서 녹산은 백악산록과 단절되었다. 셋째, 내청룡에 해당하는 경복궁 북동쪽의 풍수적 형세가 부족하여 소나무를 심어 지맥을 배양하였던 점과 임진왜란 이전 경복궁 배치도에 '소나무밭(松田)'이라고 표현된 사실에서 녹산의 원형이 된 숲의 주요 성상을 파악하였다. 발굴조사로 확인한 상수리나무, 벚나무, 느릅나무, 밤나무가 어우러진 소나무림을 원형 식생경관으로 이해할 수 있다. 녹산에는 성토로 인하여 어구가 사라지는 등 지형이 바뀌고 아까시 등 외래수종과 관상용 향나무 등이 식재되었다. 현재는 소나무림의 비중이 줄고 상수리나무림, 낙엽활엽수혼효림, 일부 가죽나무림과 버드나무림으로 구성된 숲을 이루고 있다. 넷째, '녹산(鹿山)'이라는 명칭이 신령, 장수, 영생, 왕권을 상징하는 사슴으로부터 유래된 사실을 '경복궁 녹원에서 기르던 사슴 일곱 마리 중에 한 마리가 굶어죽었다'는 대한매일신보 기사를 통해서 확인하였다.