• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.42-42
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• 2018

Two unrecognized species of Sparganium of Korea were found during the our field expeditions and phylogenetic analyses of specimens deposited in the Herbarium of Korea National Arboretum (KH). S. coreanum H. $L{\acute{e}}v$. was first reported as a new species based on the specimen (Taquet, 2150) collected from Jeju Island. It has been recognized as synonym or infraspecific taxa of S. stoloniferum and S. eurycarpum or even never recognized recently. However, phylogenetic tree showed that S. coreanum is monophyletic and has sister relationship with S. eurycarpum. Furthermore, additional distribution localities were also found by herbarium survey. Morphological characteristics and distribution information of S. coreanum will be discussed. Another Sparganium species found from Mt. Daeam is occurring either as floating or emergent. Although we could not identify this species since lack of any flowers or fruits for two year surveys, phylogenetic analyses results showed that this species belong to the clade of S. glomeratum, which is distributed in high elevation lakes and marshes of Europe, Asia, and North America. Additional survey of morphology and report will be needed.

• Animal Systematics, Evolution and Diversity
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• v.28 no.4
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• pp.297-303
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• 2012

New Korean lumbricids are described: Eisenia gaga Blakemore sp. nov. from remote Gageodo Island and E. sindo Blakemore, sp. nov. from an island at Incheon. Both are comparable to Eisenia koreana (Zicsi, 1972) from near Pyongyang, North Korea. A remarkable yet previously unrecognized similarity to American earthworm Eisenoides carolinensis (Michaelsen, 1910) is discussed, but synonymy is rejected on tenuous morphological grounds (form of nephridial vesicle bladders) with taxonomy supported by objective molecular data (mtDNA cytochrome c oxidase subunit 1 barcodes).

• Korean Journal of Plant Taxonomy
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• v.49 no.3
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• pp.203-208
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• 2019

Sparganium coreanum, a barely recognized species in Korea, was rediscovered during a field survey by the authors, who conducted a re-examination of specimens deposited in the Herbarium of the Korea National Arboretum (KH). This species was described initially by H. $L{\acute{e}}veill{\acute{e}}$ from a specimen collected by F. Taquet from Jeju-do (Taquet 2150). Subsequently, however, it was overlooked and unrecognized among South Korean flora. Several populations of S. coreanum were found in the southern part of the Korean Peninsula and on Jeju-do, although it has long been recognized as S. erectum owing to certain vegetative morphological characteristics shared between the two species, such as robust stems, a similar plant height, and globose rhizomes. However, it is distinct from S. erectum by the number of female heads on the lowest inflorescence branch and the size and shape of the fruit. In this study, we provide a detailed description, illustrations, and photographs with a revised taxonomic key for identification of Sparganium species in Korea.

• 한국균학회소식:학술대회논문집
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• 2015.11a
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• pp.41-41
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• 2015

Oomycetes belong to the kingdom Straminipila, a remarkably diverse group which includes brown algae and planktonic diatoms, although they have previously been classified under the kingdom Fungi. These organisms have evolved both saprophytic and pathogenic lifestyles, and more than 60% of the known species are pathogens on plants, the majority of which are classified into the order Peronosporales (includes downy mildews, Phytophthora, and Pythium). Recent phylogenetic investigations based on DNA sequences have revealed that the diversity of oomycetes has been largely underestimated. Although morphology is the most valuable criterion for their identification and diversity, morphological species identification is time-consuming and in some groups very difficult, especially for non-taxonomists. DNA barcoding is a fast and reliable tool for identification of species, enabling us to unravel the diversity and distribution of oomycetes. Accurate species determination of plant pathogens is a prerequisite for their control and quarantine, and further for assessing their potential threat to crops. The mitochondrial cox2 gene has been widely used for identification, taxonomy and phylogeny of various oomycete groups. However, recently the cox1 gene was proposed as a DNA barcode marker instead, together with ITS rDNA. To determine which out of cox1 or cox2 is best suited as universal oomycete barcode, we compared these two genes in terms of (1) PCR efficiency for 31 representative genera, as well as for historic herbarium specimens, and (2) in terms of sequence polymorphism, intra- and interspecific divergence. The primer sets for cox2 successfully amplified all oomycete genera tested, while cox1 failed to amplify three genera. In addition, cox2 exhibited higher PCR efficiency for historic herbarium specimens, providing easier access to barcoding type material. In addition, cox2 yielded higher species identification success, with higher interspecific and lower intraspecific divergences than cox1. Therefore, cox2 is suggested as a partner DNA barcode along with ITS rDNA instead of cox1. Including the two barcoding markers, ITS rDNA and cox2 mtDNA, the multi-locus phylogenetic analyses were performed to resolve two complex clades, Bremia lactucae (lettuce downy mildew) and Peronospora effuse (spinach downy mildew) at the species level and to infer evolutionary relationships within them. The approaches discriminated all currently accepted species and revealed several previously unrecognized lineages, which are specific to a host genus or species. The sequence polymorphisms were useful to develop a real-time quantitative PCR (qPCR) assay for detection of airborne inoculum of B. lactucae and P. effusa. Specificity tests revealed that the qPCR assay is specific for detection of each species. This assay is sensitive, enabling detection of very low levels of inoculum that may be present in the field. Early detection of the pathogen, coupled with knowledge of other factors that favor downy mildew outbreaks, may enable disease forecasting for judicious timing of fungicide applications.

• Korean Journal of Veterinary Service
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• v.23 no.4
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• pp.341-348
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• 2000

Nowadays we continue to face challenges to the safety of our foods. It seems clear that contaminated meats provide the major route of listeria monocytogenes transmission from the environment to humans. L monocytogenes is the most important species associated with disease in humans among the listeria sp. The current incidence of symptomatic listeriosis caused by L monocytogenes is uncertain. Although the number of reports in the literature on listeriosis are increasing, it is likely that they are actually unrecognized or underreported because of a lack of awareness on the part of some laboratory workers who fail to isolate or identify these organisms. To get the information of sanitary development, we survey various meats (beef, pork, etc) in Kyongbuk area. Samples were collected from local meat shop at Kyongbuk area. Total sixty six case were isolated and identified from one hundreds and seven samples. L innocua was the most abundant in listeria sp. Among U isolates, the number of isolated L innocua was 43 (65.2%). The numbers of isolated L murrayi, L welshimeri, L monocytogenes and L seeligeri were 12(18.2%), 7(10.6%), 3(4.5%) and 1(1.5%), respectively, but L grayi, L iuanouii were not Isolated.

• Korean Journal of Plant Taxonomy
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• v.46 no.4
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• pp.348-355
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• 2016

We report previously unrecognized flowering plants of Lao PDR found during a botanical survey of Lao PDR in 2015-2016. We found Phyllagathis tuberosa (C. Hansen) Cellinese & S. S. Renner (Melastomataceae) and Tolypanthus pustulatus Barlow (Loranthaceae) in the Phou Khao Khouay National Biodiversity Conservation Area (PKKNBCA), previously only known from type localities in Thailand. This is the first report of Phyllagathis tuberosa and the genus Tolypanthus in the Lao PDR. We also found Lonicera bournei Hemsl. (Caprifoliaceae) in the proposed Phosabous National Protected Area (PNPA) of Xiangkhoang, neglected with regard to the flora of Laos since its initial report in early 20th century. The current report will augment our understanding of the floristic relationships among SE Asian countries and will help elucidate the biodiversity of Lao PDR.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.38 no.4
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• pp.1-11
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• 2020

Noksan is a green area in the form of a hill located inside Gyeongbokgung Palace, unrecognized as a cultural heritage space. This study analyzed the literature and the actual site to derive its landscape meaning by examining the background for the formation of Noksan and how it changed. As a result, the identity of Noksan was related to the geomagnetic vein, pine forest, and deers, and the following are its landscape meaning. First, several ancient maps, including the 「Map of Gyeongbokgung Palace」 depicted the mountain range continuing from Baegaksan(Bugaksan) Mountain to areas inside Gyeongbokgung Palace, and Noksan is a forest located on the geomantic vein, which continues to Gangnyeongjeon Hall and Munsojeon Hall. On Bukgwoldo(Map of Gyeongbokgung Palace), Noksan is depicted with Yugujeong Pavilion, Namyeogo Storage, office for the manager of Noksan, the brook on north and south, and the wall. It can be understood as a prototypical landscape composed of minimal facilities and the forest. Second, the northern palace walls of Gyeongbokgung Palace were constructed in King Sejong's reign. The area behind Yeonjo(king's resting place) up to Sinmumun Gate(north gate of the palace) was regarded as the rear garden when Gyeongbokgung Palace was constructed. However, a new rear garden was built outside the Sinmumun Gate when the palace was rebuilt. Only Noksan maintained the geomantic vein under the circumstance. However, the geographical features changed enormously during the Japanese colonial era when they constructed a huge official residence in the rear garden outside the Sinmumun Gate and the residence of the governor-general and road in the site of the Blue House. Moreover, Noksan was severed from the foothill of Baegaksan Mountain when 'Cheongwadae-ro(road)' was constructed between the Blue House and Noksan in 1967. Third, the significant characteristics and conditions of the forest, which became the origin of Noksan, were identified based on the fact that the geomatic state of the northeastern side of Gyeongbokgung Palace, the naecheongnyong area in geomantic terms(the innermost 'dragon vein' among the veins that stretched out from the central mountain toward the left side), and they planted pine trees to reinforce the 'ground vein' and the fact that it was expressed as the 'Pine Field' before the Japanese Invasion of Korea in 1592. The pine forest, mixed with oaks, cherries, elms, and chestnuts, identified through the excavation investigation, can be understood as the original vegetation landscape. Noksan's topography changed; a brook disappeared due to mounding, and foreign species such as acacia and ornamental juniper were planted. Currently, pine trees' ratio decreased while the forest is composed of oaks, mixed deciduous trees, some ailanthus, and willow. Fourth, the fact the name, 'Noksan,' came from the deer, which symbolized spirit, longevity, eternal life, and royal authority, was confirmed through an article of The Korea Daily News titled 'One of the seven deers in Nokwon(deer garden) in Gyeongbokgung Palace starved to death.'