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Transcriptome Analysis in the Midgut of the Earthworm (Eisenia andrei) using Expressed Sequence Tags

  • Lee, Myung-Sik;Cho, Sung-Jin;Lee, Jong-Ae;Moon, Joo-Sik;Cho, Hyun-Ju;Park, Bum-Joon;Kim, Seong-Ki;Choo, Jong-Kil;Park, Soon-Cheol
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.144-144
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    • 2003
  • In order to understand the expression profile of earthworm midgut, we analyzed 1255 expressed sequence tags (ESTs) derived from earthworm midgut cDNA library. Among 1255 ESTs analyzed, 537 (42.8%) ESTs represented 231 unique genes of which 168 ESTs were singletons and 63 ESTs represented by two or more ESTs. A total of 571 unknown ESTs showed no significant homology. The remaining 147 (11.7%) ESTs whose lengths were less than 150 bp were removed. Among 231 identified unique genes, 168 genes (72.7%) were sequenced only once. (omitted)

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