• 한국전자현미경학회:학술대회논문집
• /
• 2002.05a
• /
• pp.25-26
• /
• 2002

• Journal of Ginseng Research
• /
• v.26 no.4
• /
• pp.213-218
• /
• 2002

Crystalline calcium oxalate occur throughout near)y all plants species in five major forms; styloids, druses, raphids, prisms and sands. These crystals are known to be distributed in specific tissue such as cortex, xylem, phloem, cambium and epidermis. This research was undertaken to identify the occurrence, type, location and ultrastructure of druse crystals in Panax ginseng. In situ visualization, conventional light microscopy, histochemistry and scanning electron microscopy were applied for these purposes. Druse crystals in ginseng were identified as calcium oxalate by silver nitraterubeanic acid histochemistry. Calcium oxalate crystals are observed in nearly all plant organs such as leaf, petiole, peduncle, stem, rhizome, tap root and lateral root except fine root. Most frequent observation of crystals in the leaf and rhizomes were noticed. Three different types of calcium of oxalate druse crystals were identified by scanning electron microscopy.

• Korean Journal of Ichthyology
• /
• v.17 no.2
• /
• pp.98-104
• /
• 2005

Integumentary structures of the chub mackerel (Scomber japonicus) were examined by light and transmission electron microscopy. The integument of the fish was composed of epidermal and dermal layers. The epidermal thickness is about $20{\mu}m$ in TL 35~40 cm fish. The epidermal layer could be classified into superficial, intermediate, and basal layers by morphology and position of the supporting cells. The epidermal layer also possessed mucous cells, club cells and chloride cells. The area of secretory cells, including mucous and club cells, is about 23% of the epidermal layer. The mucous materials were identified as sulfated glycoprotein, neutral and acid in nature. Club cells had a large central vacuole and rough endoplasmic reticula in the cytoplasm. Chloride cells had numerous tubular mitochondria in the cytoplasm. The dermal layer consists mainly of collagenous fiber, and it contains fibrocytes, pigment cells and cycloid scales.

• The Korean Journal of Malacology
• /
• v.13 no.2
• /
• pp.175-184
• /
• 1997

A histochemical and ultrastructural study on the stomach epithelium of a land snail Nesiohelix samarangae was carried out during the period of June 1996 to May 1997. The stomach epithelium is simple columnar dpithelium and is consisted of three types of columnar cells, Type 1 cell which is majority in number has a brush border with microvilli on the free surface of the cell and contains numerous secretory granules supposed to be neutral mucopolysaccharide. Type 2 cell, elongated conical in shape. is rarely found in the dpithelium. This cell also has a brush border with microvilli on its free surface and contains well developed rough surfaced endoplasmic reticulum, Golgi bodies, and secretory granules in various electron densities. This cell seems to produce both of acid and neutral mucopolysaccharides. Type 3 cell, which is morphologically similar to the Type 1 cell, has microvili and cilia on the free surface and exists in group only in the limited regions of the stomach.

• The Korean Journal of Malacology
• /
• v.20 no.1
• /
• pp.7-16
• /
• 2004

Histochemical and ultrastructural studies on the salivary gland and salivary duct of a land snail Nesiohelix samarangae were conducted to observe structural characteristics and function. The salivary gland consisted of one type of epithelial cell, one type of supporting cell, and six types of gland cells. Four out of six gland cell types were histochemically identified on these secretions. The one secreted acid mucopolysaccharide and the other three secreted neutral mucopolysaccharide. The salivary duct epithelium had only one type of columnar cell with microvilli on its luminal surface. The basal protoplasmic membranes of the epithelial cells were deeply infolded so many times all along the cell bases.

• Applied Microscopy
• /
• v.31 no.4
• /
• pp.325-331
• /
• 2001

Integumentary structures of the stone flounder, Karefus bicoloratus were examined by means of the light and transmission electron microscopy. Stratified epidermal layer consists of supporting cells, unicellular glands and granular cells. The epidermal layer could be classified into superficial, intermediated and basal layer by morphology and structure of the supporting cells . The cytoplasm of supporting cells is divided into cortex and medullar part. In the cortex microfilaments are well developed. Mucous cells of unicellular gland were observed in the superficial and intermediated layer of the epidermis. The mucous materials were identified as glycoprotein of neutral and carboxylated mucosubstance by histochemical methods. Club cell has well developed smooth endoplasmic reticula and Golgi complex in the cytoplasm. Granular cells were observed in the intermediated and basal layer, and the cytoplasm is occupied with membrane-bounded granules of electron dense. Three types of pigment cells could be distinguished with electron density of cytoplasmic inclusions. Nerve myelins were observed near the pigment cells.

• Journal of Plant Biology
• /
• v.38 no.1
• /
• pp.115-119
• /
• 1995

In order to clarify the relationship between the activity and age of vacular cambium in the rhizome of Botrychium ternatum, the cambia of the 6th and 10th internodes from its shoot apex were compared ultrastructurally and histochemically. The cambium in the 6th internode had two to four radially arranged cells in each row, and the cells are radially flattened and had thiner tangential walls than radial. The cambial cells were ultrastructurally characterlized by the presence of a large vacuole, few storage materials such as starch grains and lipid droplets, and various cell organelles such as mitochondria, endoplasmic reticulums and dictyosomes in cytoplasm. On the other hand, the cells of cambium in the 10th internode showed irregular arangement and somewhat enlargement in radial plane. The cells were filled with numerous storage materials and small vacuoles and had few cell organelles except for mitochondria. In addition, the activity of acid phosphatase appeared in the cambium of the 6th internode but of the 10th. From the above results, it is clear that the vascular cambium of the 6th internode was active and that of the 10th was dormant. Thus we reached a conclusion that the rhizome of '||'&'||'trychium ternatum has determinate cambium.

• Applied Microscopy
• /
• v.34 no.2
• /
• pp.83-93
• /
• 2004

Integumentary structures of the rockfish, Sebastiscus tertius were examined by means of the light and transmission electron microscopy. Stratified epidermal layer consists of supporting cells, unicellular glands, granular cells and mitochondria-rich cells. The epidermal layer could be classified into superficial, intermediate and basal layer by morphology and structure of the supporting cells. Mucous cells of unicellular gland were observed in the superficial and intermediate layer of the epidermis. The mucous materials were identified as acidic and carboxylated mucosubstance by histochemical methods. Club cell has well-developed central vacuole, rough endoplasmic reticula and Golgi complex in the cytoplasm. Granular cells were observed in the superficial layer and contained numerous granules of high electron density. Mitochondria-rich cells are characterized by well-developed microfilaments in cortex and numerous tubular mitochondria in medullar cytoplasm. Three types of pigment cells in the dermal layer could be distinguished with electron density of cytoplasmic inclusions.

• The korean journal of orthodontics
• /
• v.24 no.3 s.46
• /
• pp.569-586
• /
• 1994

The purpose of this study is to investigate the effect of loss of incisal function on the thickness, growth activities, ultrastructure of the condylar cartilage and on the muscle fibers of masseter superlicialis, anterior belly of digastric muscle in the growing rats. 37 day-old-rats of which incisors had been trimmed every day received soft diet from weaning and were studied by the autoradiography, electron microscopy and muscle histochemistry. The results obtained were as follows : The thickness of the fibrous, proliferative layer in superior, posterosuperior portion of the condylar cartilage was significantly(p<0.01) reduced in experimental groups and the decrease rate of fibrous layer thickness was greater in posterosuperior portion than in superior portion of cartilage and was greater than in proliferative layer. In normal group, more cells of posterosuperior portion moved more rapidly towards the medullary cavity. In experimental group, the labelling index of posterosuperior portion was decreased in proliferative layer at 2 hours, in transitional layer at 1, 2 days, in hypertrophic layer at 4 days after injection relative to posterosuperior portion of control group. But labelling index of superior portion was not different from that of control group at all time course after injection. From the muscle histochemistry, the diameter of type IIB fibers in masseter superficialis muscle, type IIA, type IIB fibers in anterior belly of digastric muscle decreased significantly(p<0.01) relative to controls in experimental group. From electron microscopic study, in the fibrous layer of the posterosuperior portion of condylar cartilage in normal group, many fibroblast like cells near the joint cavity showed extensive remodelling activities in ultrastructure. There was no morphological changes between experimental and control group in all cartilage cell layers of superior portion but cells near the joint cavity in fibrous layer of posterosuperior portion of experimental group showed morphologically inactive state relative to control group.

• Applied Microscopy
• /
• v.18 no.2
• /
• pp.205-217
• /
• 1988

An ultrastructural study of hepatocyte proliferation in the regenerating rat liver has been made by means of the partial hepatectomy. And electron microscopic histochemistry of hepatocyte in the regenerating rat liver is studied through alkaline phosphatase reaction. The results are as follows: 1. When the regeneration of rat liver is induced by the partial hepatectomy, the prominent ultrastructural characteristics of hepatocyte are changes of the distribution of chromatin in nucleus, increase of the number of mitochondria and decrease of the size of them, development of rough endoplasmic reticulum, and transient decrease of glycogen granules in cytoplasm. 2. Alkaline phosphatase reaction products are appeared in the nucleus or rough endoplasmic reticulum of hepatocyte during the initial regeneration of liver as 24, 48 and 72 hour groups after partial hepatectomy. And these positive reaction are mainly increased in cytoplasm and plasma membrane of hepatocytes during 1, 2 and 3 week groups after partial hepatectomy. As 4 weeks passed after partial hepatectomy, these positive reaction is located in the sinusoidal epithelial cells or erythrocytes. With above results, we concluded that alkaline phosphatase was synthesized in the rough endoplasmic reticulum bounded ribosomes of regenerating hepatocyte, was transported to the plasma membrane of them, and then was transported in blood by the way sinusoidel epithelial cells.