• Title/Summary/Keyword: ultrastructure.

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Ultrastructure of Spermatozoa of a Korean Bitterling, Acheilognathus koreensis (Pisces, Cyprinidae) (한국산 잉어과어류 칼납자루(Acheilognathus koreensis) 정자의 미세구조)

  • Kim, Kgu-Hwan;Kim, Jeong-Ki;Hwang, Ki-Ju
    • Korean Journal of Ichthyology
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    • v.19 no.4
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    • pp.286-291
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    • 2007
  • The bitterling, Acheilognathus koreensis spermatozoon has been examined by electron microscopy. The epididymal spermatozoa of A. koreensis are representing typical characteristic of cyprinid spermatozoa including the lateral insertion of flagellum, the organization of centriolar complex in shallow nuclear fossa and the asymmetrical arrangement of mitochondria. The sperm mid-piece contains a large mitochondrion characteristic enclosed by membranous vesicles. The mitochondria aspect is different from that of other cyprinid spermatozoa, which their mitochondria have a conventional aspect and never fuse to form a mitochondrial derivative. In term of sperm evolution, the fused mitochondria are regarded as the apomorphic character in comparison with the separate mitochondria. The single mitochondrion is not found in cyprinid spermatozoon except for Rodeus and Pungtungia.

Effect of Neuromuscular Electrical Stimulation(NMES) on the Ultrastructure of Skeletal Muscle in Rats (신경근전기자극이 흰쥐 골격근의 미세구조에 미치는 영향)

  • Park, Jang-Sung;Park, Chun-Man
    • Journal of the Korean Academy of Clinical Electrophysiology
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    • v.1 no.1
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    • pp.57-72
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    • 2003
  • This study conducts electrical stimulation to male white rat of Spargue-Dawley which is 7 weeks, has the weight of 240 g and is seemingly healthy for one or two weeks by means of neuromuscular electrical stimulator in order to examine the effects of neuromuscular electrical stimulation on its gastrocnemius, measures change of weight of gastrocnemius, serum and enzyme activity and then obtains the following conclusions. There is little difference in AST and CPK of weight and serum of gastrocnemius after one or two weeks of conducting neuromuscular electrical stimulation in all experimental groups. On the one hand, as a result of histochemical observation, NMES I group showed hypertrophy of perimysium and increase of sectional diameter of muscle fiber compared to comparison group, but NMES II group showed a similar result to comparison group. When ultrasubstructure was observed under electron microscope, I-type muscle fiber of NMES I group showed well-arranged mitochondria and it was similar to comparison group. II-type muscle fiber showed a large quantity of glycogen granules within sarcoplasmatic and the extension of luminal of T-tubule. I-type muscle fiber of NMES II group had small mitochondria and showed the vacuolar degeneration of mitochondria and extended T-tubule. II-type muscle fiber showed the extension of agranule cytoplasma reticulum with T-tubule and the reduction of amount of glycogen granule within partial sarcoplasmatic.

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Ultrastructure of the Ampullate Gland in the Orb Web Spider, Nephila clavata L. Koch II. Sac and Tail Portion of the Large Ampullate Gland (무당거미(Nephila clavata L. Koch) 병상선(甁狀腺)의 미세구조(微細構造) II. 대병상선(大甁狀腺)의 분비낭(分泌囊)과 말단분비부(末端分泌部))

  • Moon, Myung-Jin;Kim, Chang-Shik;Kim, Woo-Kap
    • Applied Microscopy
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    • v.18 no.2
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    • pp.91-101
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    • 1988
  • The ultrastructure of the sac and tail portion of the large ampullate gland and production of the silk materials in the orb web spider, Nephila clavata L. Koch, are studied with electron microscope. Large ampullate glands, the largest glands among the seven kinds of silk glands in this species, are composed of three parts which are the excretory duct, the storage sac and the convoluted tail. The wall of the sac is composed of a single layer of columnar epithelial cells. In the cytoplasm of these cells several kinds of the secretory granules, which are commonly associated with the rough endoplasmic reticula and had characteristic crystalloid, are seen. According to the morphology and internal textures of these granules, the sac portion is subdivided into proximal(connected with the convoluted tail) and distal(connected with excretory duct) portion. Between these two portions, the proximal portion is longer than the distal by four times. Silk materials, being synthesized to the shape of secretory granules, within the glandular epithelial cell of the tail portion are released to the inner cavity by the mechanism of the eccrine secretion. These secretory granules are originated from the rough endoplasmic reticula of the glandular epithelial cells, whereas no Golgi complexes has been found in any of the cells which have been examined.

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Ultrastructure of the Ampullate Gland in the Orb Web Spider, Nephila clavata L. Koch IV. Secretory Portion of the Small Ampullate Gland (무당거미(Nephila clavata L. Koch) 병상선(甁狀腺)의 미세구조(微細構造) IV. 소병상선(小甁狀線)의 선분필부(腺分泌部))

  • Moon, Myung-Jin;Kim, Woo-Kap
    • Applied Microscopy
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    • v.19 no.1
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    • pp.59-69
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    • 1989
  • Ultrastructure of the secretory portion of the small ampullate gland in the orb web spider, Nephila clavata, has been investigated using the electron microscope. The secretory portion of this gland comprise two different regions which are a S-shaped storage sac and a long, convoluted tail. By the internal textures of the secretory granules, the sac is subdivided into two regions ; the proximal region near the tail and the distal region near the duct. Commonly single layered connectives cover the basal portion of the sac epithelium, and apical portion of the epithelial cells is occupied by the thick cuticles. Within the epithelial cells of both the proximal and distal region, several types of the secretory granules surrounded by a limiting membrane and had characteristic crystalloid are scattered throughout the cytoplasm. The granular size and its electron densities are not coincide with each other according to the maturation level of the granules. The wall of the tail is composed of single layer of columnar epithelial cells, and their nuclei are found at the basal portion of the cells. Dissimilar to the epithelial cells of the sac, apical cuticles are not found at this portion. In the cytoplasm of these cells, numerous secretory granules, synthesized from the rough endoplasmic reticula commonly and had fine fibrous materials, are found. At the cell surface bordering the lumen, microvilli are seen, arid along the cellular boundaries specialized septate junctions and desmosomes appeared.

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Ultrastructure of the Ampullate Glands in the Orb Web Spider, Nephila clav ata L. Koch III. Excretory Duct of the Small Ampullate Gland (무당거미(Nephila clavata L. Koch) 병상선(甁狀腺)의 미세구조(微細構造) III. 소병상선(小甁狀腺)의 분필관(分泌管))

  • Moon, Myung-Jin;Kim, Woo-Kap
    • Applied Microscopy
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    • v.19 no.1
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    • pp.49-58
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    • 1989
  • Ultrastructure of the excretory duct of the small ampullate gland in the orb web spider, Nephila clavata L. Koch are studied with light and electron microscopes. The small ampullate glands, located near the midline portion of the abdominal cavity, are connected with the spigots(large spinning tubes) on the middle spinnerets and composed of three parts which are the excretory duct, the storage sac and the convoluted tail. The excretory duct of this gland is enclosed by a thin layer of the outer connective tissues. By the morphology of the apical cuticles and internal textures of the epithelial cells, the duct is subdivided into two regions which are proximal duct region near the sac and distal duct region near the spinnerets. At the distal region of the ducts, the subcuticle which had the function of water removal form the progenetive silk material is well developed, whereas at the proximal region this cuticle disappeared and instead of these, endocuticle is developed. Moreover the epithelium of the distal duct region is composed of columnar epithelial cells, but at the proximal region the epithelium is changed to squamous or cuboidal forms. In the cytoplasm of the epithelial cells, rough endoplasmic reticula, Golgi comlexes and large secretory vesicles related to the production of the cuticular materials are well developed. And between the adjacent epithelial cells, specialized septate junction and desmosomes are formed along the plasma membrane.

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