• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.406-411
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• 2005

Fermented materials with enterobacteria isolated from fusiform fish, have strong anti-angiogenesis effect and anti-cell adhesion effect. PLM-f74 got from 74th fraction of size exclusion chromatography from fermented material, showed strong anti-cell adhesion effect between HUVECs and U937 monocytic cell. Adhesion of U937 cell to HUVEC stimulated with IL-1b was clearly inhibited by PLM-f74 in a dose-dependent manner by 12.1, 21.2, 50.9, and 78.2%, when U937 cells treated with each of the PLM-f74 and stimulated with PMA (100 mg/L) was added onto untreated and unstimulated HUVECs, adhesion was observed by 15.8, 31.9, 70.8, and 102%, when both cell types were pretreated with PLM-f74, the adhesion was prominently decreased by 83.7, 99.2, 110, and 120.8%, with 0.74, 3.7, 7.4, and 18.5ug/mL of PLM-f74, respectively. PLM-f74, also, reduced IL-1-stimulated HUVEC expression of adhesion molecules, VCAM-1, ICAM-1, and E-selectin dose-dependently by ELISA method.

• 한국해양바이오학회지
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• 제1권3호
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• pp.156-162
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• 2006

The halophilic enterobacteria, Enterobacteria cancerogenus, was isolated from the intestines of the fusiform fish (Trachurus japonicus) to yield a protein-like material termed PLM-f74. PLM-f74 was characterized by strong inhibition ratios to angiogenesis (82.8% at the concentration of $18.5{\mu}g/mL$) and elevated antioxidative capacities with low toxicity. The PLM-f74 is a glycoprotein comprised of saccharides and amino acids. PLM-f74 inhibited non-activated U937 monocytic cell adhesion to HUVECs activated with IL-$1{\beta}$ by 78.0%, and the adherence of U937 cells treated with the PLM-f74 and stimulated with IL-$1{\beta}$ to unstimulated HUVECs decreased by 102%. When both cell types were pretreated with PLM-f74, the adhesion of U937 cells to IL-$1{\beta}$ stimulated HUVECs was completely suppressed by 121% at a concentration of 18.5 ug/mL. PLM-f74 blocked signal pathways from VEGFR2, PI3K, ${\beta}$-catenin and VE-cadherin to NF-kB based on western bolt analysis. And also inhibited IL-1-stimulated HUVEC expression of the adhesion molecules, ICAM-1 by 40%, VCAM-1 by 60%, and E-selectin by 70% at the same concentration noted above. New anti-angiogenic and anti-cell adhesion materials showing elevated antioxidative capacities and non-toxicity may be expected from these results.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1544-1553
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• 2006

The halophilic enterobacteria, Enterobacteria cancerogenus, was isolated from the intestines of the fusiform fish (Trachurus japonicus) to yield a protein-like material termed PLM-f74. PLM-f74 was characterized by strong inhibition ratios to angiogenesis (82.8% at the concentration of $18.5{\mu}g/ml$) and elevated antioxidative capacities with low toxicity. The PLM-f74 is a glycoprotein comprised of saccharides and amino acids. PLM-f74 inhibited cell adhesion that non-activated U937 monocytic cell adhesion to HUVECs activated with $IL-1{\beta}$ by 78.0%, and the adherence of U937 cells treated with the PLM-f74 and stimulated with $IL-1{\beta}$ to unstimulated HUVECs decreased by 102%. When both cell types were pretreated with PLM-f74, the adhesion of U937 cells to $IL-1{\beta}$-stimulated HUVECs was completely suppressed by 121% at a concentration of $18.5{\mu}g/ml$. PLM-f74 blocked signal pathways from VEGFR2, PI3K, ${\beta}$-catenin, and VE-cadherin to NF-kB, based on western bolt analysis. It also inhibited IL-l-stimulated HUVEC expression of the adhesion molecules, ICAM-l by 40%, VCAM-l by 60%, and E-selectin by 70% at the same concentration noted above. New anti-angiogenic and anti-cell adhesion materials showing elevated antioxidative capacities, and non-toxicity may be expected from these results.

• 대한조선학회지
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• 제43권2호
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• pp.18-22
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• 2006

• 산업공학
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• 제19권4호
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• pp.333-341
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• 2006

RFID (Radio Frequency Identification) is a technology that can input identification information to microchip and make goods, animals, persons recognized, chased, and managed using radio frequency, and is founded on the core technology of ubiquitous environment of the future. In this paper, we propose a RFID integrated system designed to manage the lifecycle of an individual product efficiently. The proposed system can enable traceability and visibility of items through their entire life by integrating distribution and banking information on the basis of EPCglobal Network. It may provide the infra of Digital Manufacturing and RTE (Real Time Enterprise) and effective information sharing structure with existing legacy system (ERP, CRM, SCM) by real time.

• 자원환경지질
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• 제47권5호
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• pp.479-488
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• 2014

최근 석면의 효과적인 관리를 위해 산업적 이용 규제 및 석면포함물질 처리 뿐 아니라 자연발생석면의 이해와 정화 필요성도 제기되고 있다. 그러나 국내에서 암석 내 산출되는 자연발생석면의 특성이나 풍화작용을 거쳐 토양 내 잔류하는 자연발생석면에 대한 기존 연구는 미비한 상황이다. 따라서 이 연구에서는 변성퇴적암류가 기반암인 충남 서산 대로리 일대의 토양 내 자연발생석면의 존재를 확인하고 석면형(asbestiform) 광물의 산출양상 및 광물학적 특성을 통해 석면오염토양의 효과적인 정화 방안에 대해 알아보고자 하였다. 토양의 입도 분리 및 광물학적 분석을 실시한 결과, 토양 내 무게 백분율은 모래가 26~93%, 미사가 4~23%, 점토가 3~70%이며, 토성은 사토(sand), 사양토(sandy loam), 사질 식토(sandy clay), 식토(clay) 등 다양하게 나타났다. 연구 결과 모든 시료에서 석면형 투각섬석과 석면형 양기석이 함께 산출되었고 석면의 함량은 평균 1.5%로 미국 EPA 기준(1% 이상)에 따라 석면 포함물질로 분류되었다. 모래에 존재하는 투각섬석은 벽개가 발달한 주상 형태로 평균 길이 $288{\mu}m$, 폭 $69{\mu}m$, 종횡비 4.1:1의 특징을 보인 반면, 미사에 존재하는 투각섬석은 평균 길이 $49.2{\mu}m$, 폭 $5.8{\mu}m$로 크기는 더 작고 종횡비가 8.5:1인 개별섬유 형태로 나타났다. 이는 투각섬석이 풍화작용을 받으면서 종횡비는 커지고 형태는 비산이 가능한 개별 섬유로 바뀐 것으로 사료된다. $5{\mu}m$ 이상 크기의 석면형 투각섬석과 석면형 양기석의 함량은 토양 중 미사에서 가장 높게 나타났으며 이를 통해 석면오염토양 정화 시 입도 분리를 이용한 선택적인 석면 제거의 가능성을 확인하였다.