• Clinical and Experimental Reproductive Medicine
• /
• 제38권1호
• /
• pp.24-30
• /
• 2011

Objective: The objectives of this study were to analyze efficacy of immature and mature mouse oocytes after vitrification and warming by applying various combinations of cryoprotectants (CPAs) and/or super-rapid cooling using slush nitrogen ($SN_2$). Methods: Four-week old ICR female mice were superovulated for GV- and MII-stage oocytes. Experimental groups were divided into two groups. Ethylene glycol (EG) only group: pre-equilibrated with 1.5 M EG for 2.5 minutes and then equilibrated with 5.5 M EG and 1.0 M sucrose for 20 seconds. EG+dimethylsulfoxide (DMSO) group: pre-equilibrated with 1.3 M EG+1.1 M DMSO for 2.5 minutes and equilibrated with 2.7 M EG+2.1 M DMSO+0.5 M sucrose for 20 seconds. The oocytes were loaded onto grids and plunged into $SN_2$or liquid nitrogen ($LN_2$). Stored oocytes were warmed by a five-step method, and then their survival, maturation, cleavage, and developmental rates were observed. Results: The EG only and EG+DMSO groups showed no significant difference in survival of immature oocytes vitrified after warming. However, maturation and cleavage rates after conventional insemination were greater in the EG only group than in the EG+DMSO group. In mature oocytes, survival, cleavage, and blastocyst formation rates after warming showed no significant difference when EG only or EG+DMSO was applied. Furthermore, cleavage and blastocyst formation rates of MII oocytes vitrified using $SN_2$ were increased in both the EG only and EG+DMSO groups. Conclusion: A combination of CPAs in oocyte cryopreservation could be formulated according to the oocyte stage. In addition, $SN_2$ may improve the efficiency of vitrification by reducing cryoinjury.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2000년도 추계학술대회논문집B
• /
• pp.192-198
• /
• 2000

Three dimensional thermal cycle analysis of the plunger is carried out in repeated forming process of the TV glass, which is continued work of two dimensional analysis where an efficient method has been proposed. The plunger undergoes temperature fluctuation during a cycle due to the repeated contact and separation from the glass, which attains a cyclic steady state having same temperature history at every cycle. Straightforward analysis of this problem brings about more than 90 cycles to get reasonable solution. An exponential function fitting method is proposed, which finds exponential function to best approximate temperature values of 3 consecutive cycles, and new cycle is restarted with the fitted value at infinite time. Number of cases are analyzed using the proposed method and compared to the result of straightforward repetition, from which one finds that the method always reaches nearly convergent solution within $9{\sim}12$ cycles, but turns around afterwards without further convergence. Two step use is found most efficient, in which the exponential fitting is carried out fer the first 12 cycles, followed by simple repetition, which shows fast convergence expending only 6 additional cycles to get the accuracy within 2 error. This reduces the computation cycle remarkably from 90 to 18, which is 80% reduction. From the parametric studies, one reveals that the overall thermal behavior of the plunger in terms of cooling parameters and time is similar to that of 2 dimensional analysis.

• 한국기계가공학회지
• /
• 제21권3호
• /
• pp.1-11
• /
• 2022

In this study, an electric resistance dual-spot welding process using a copper electrode inserted in a heating electrode is suggested for the spot welding of AZ31 magnesium sheets. This spot-welding process involves two heating methods for welding at the interfacial zone between the magnesium sheets, one of which is the heating method by thermal conduction from the heating electrode heated by the welding current induced to the steel electrode, and the other heating method uses the electric resistance between the contacted surfaces of the two sheets by the welding current induced to the copper electrode. This welding process includes the welding variables, such as the current induced in the heating electrode and the copper electrode, and the outer diameters of the heating electrode. This is because the heat conducted from the heating electrode can be maintained at a higher temperature in the welding zone, which has a slow cooling effect on the nugget of the melted metal after the welding step. The pressure exerted during the pressing of the magnesium sheets by the heating electrode can be increased around the nugget zone at the spot-welding zone. Thus, it not only reduces the warping effect of the elastoplastic deformation of sheets, but also the corona bond can make it less prone to cracking at the welded zone, thereby reducing the number of nuggets expelled out of the corona bond. In conclusion, it was known that an electric resistance dual spot welding process using the copper electrode inserted in the heating electrode can improve the welding properties in the electric resistance spot welding process of AZ31 magnesium sheets.

• 생물환경조절학회지
• /
• 제23권3호
• /
• pp.181-191
• /
• 2014

Modern commercial greenhouse requires the use of advanced climate control system to improve crop production and to reduce energy consumption. As an alternative to classical sensor-based control method, this paper introduces a model-based control method that consists of two models: the predictive model and the evaluation model. As a first step, this paper presents straightforward models to predict the effect of natural ventilation in a greenhouse according to meteorological factors, such as outdoor air temperature, soil temperature, solar radiation and mean wind speed, and structural factor, opening rate of roof ventilators. A multiple regression analysis was conducted to develop the predictive models on the basis of data obtained by computational fluid dynamics (CFD) simulations. The output of the models are air temperature drops due to ventilation at 9 sub-volumes in the greenhouse and individual volumetric ventilation rate through 6 roof ventilators, and showed a good agreement with the CFD-computed results. The resulting predictive models have an advantage of ensuring quick and reasonable predictions and thereby can be used as a part of a real-time model-based control system for a naturally ventilated greenhouse to predict the implications of alternative control operation.

• 한국동물번식학회:학술대회논문집
• /
• 한국동물번식학회 2001년도 춘계학술발표대회
• /
• pp.5-5
• /
• 2001

This study was to test whether Hanwoo in vitro matured oocytes can be successfully cryopreserved by a new vitrification procedure using MVC method. For the vitrification, oocytes were pretreated in 10% ethylene glycol (EG10) for 5-10 min, exposed in EG30 for 30 sec, each oocytes were individually put on the inner wall of 0.25 $m\ell$ straw, and then straws were directly plunged into L$N_2$. Thawing was taken by 4-step procedures [1.0 Msucrose (MS), 0.5 MS, 0.25 MS, and 0.125 MS] at 37$^{\circ}C$. In vitro developmental capacity (survival, cleavage ($\geq$2-cell) and blastocyst rates) in vitrified group was no significant difference compared to that in other treatment groups (exposed; 100.0, 74.4, 32.3% and control; 100.0, 78.3, 36.3%): high mean percentage of oocytes (91.2%) was survived, 69.4% of them were cleaved and 27.9% of cleaved embryos were developed to blastocyst. Especially, after transfer of in vitro developed embryos in vitrified group, four of six recipient animals were found to pregnant and three of them were ongoing pregnant by manual palpation at 250 days after transfer. However, among them, two healthy female calves (23 and 25kg) were born. This result demonstrates that MVC method is very appropriate freezing method for the Hanwoo in vitro matured oocytes and that ovum bank can be maintained efficiently by MVC cryopreservation method.

• 한국추진공학회:학술대회논문집
• /
• 한국추진공학회 2004년도 제22회 춘계학술대회논문집
• /
• pp.165-172
• /
• 2004

LE-7A is the main engine of the H-IIA launch vehicle. Under its development, the nozzle suffered from two troubles during startup and shutdown transients of the engine. One is a large side load, which damages the actuator of the nozzle, and the other is damage on regenerative cooling tubes due to high heat load. It has been considered that these problems are caused by a peculiar separation pattern called Restricted Shock Separation (RSS). RSS is observed in several rocket nozzles, for example, LE-7A nozzle, Vulcain nozzle and so on. Their contours are not conventional truncated perfect (TP) nozzle - LE-7A nozzle is a compressed truncated perfect (CTP) nozzle and Vulcain nozzle is a thrust optimized (TO) nozzle. Although it is believed that the occurrence of RSS is affected by the nozzle contour, the mechanisms are not clarified sufficiently yet. In the present paper, a parametric numerical study is carried out to investigate the mechanisms of the occurrence of RSS in CTP nozzles during startup transient. The results show that RSS is caused by the adverse pressure gradient downstream of the Mach disk. The adverse pressure gradient is caused by the interaction of the pressure wave and Mach disk. The method to avoid the occurrence of RSS is also examined. A small step inside the nozzle affects the position of the separation point and prevents RSS. The result shows that the possibility that RSS can be suppressed by controlling the position of the separation point.

• 천문학회보
• /
• 제36권1호
• /
• pp.64.1-64.1
• /
• 2011

MIRIS (Multipurpose Infra-Red Imaging System), is a small infrared space telescope which is being developed by KASI, as the main payload of Science and Technology Satellite 3 (STSAT-3). Two wideband filters (I and H) of the MIRIS enables us to study the cosmic infrared background by detecting the absolute background brightness. The narrow band filter for Paschen ${\alpha}$ emission line observation will be employed to survey the Galactic plane for the study of warm ionized medium and interstellar turbulence. The opto-mechanical design of the MIRIS is optimized to operate around 200K for the telescope, and the cryogenic temperature around 90K for the sensor in the orbit, by using passive and active cooling technique, respectively. The engineering and qualification model of the MIRIS has been fabricated and successfully passed various environmental tests, including thermal, vacuum, vibration and shock tests. The flight model was also assembled and is in the process of system optimization to be launched in 2012 by a Russian rocket. The mission operation scenario and the data reduction software is now being developed. After the successful mission of FIMS (the main payload of STSAT-1), MIRIS is the second Korean space telescope, and will be an important step towards the future of Korean space astronomy.

• Journal of Advanced Marine Engineering and Technology
• /
• 제8권1호
• /
• pp.17-36
• /
• 1984

Since 1973, the competition on the development of fuel saving type internal combustion engines has become severe by the two times oil shock, and new type engines are reported every several months. Whenever these new type engines are developed, new designs are required and they will be offered in the market after performing the endurance test for a long time. But the engine market is faced with a heavy burden of finance, as the developing of a new engine requires tremendous expenses. For this reason, the computer simulation method has been lately developed to cope with it. The computer simulation method can be available to perform the reasonable research works by the theoretical analysis before carrying out practical experiments. With these processes, the developing expenses are cut down and the period of development is curtailed. The object of this study is the development of simulation computer program for the small naturally aspirated four-stroke diesel engine which is intended to product by the original design of our country. The process of simulation is firstly investigated for the ideal engine cycle, and secondly for the real engine cycle. In the ideal engine cycle, each step of the cycle is simulated by the energy balance according to the first law of thermodynamics, and then the engine performance is calculated. In the real cycle imulation program, the injection rate, the preparation rate and the combustion rate of fuel and the heat transfer through the wall of combustion chamber are considered. In this case, the injection rate is supposed as constant through the crank angle interval of injection and the combustion rate is calculated by the Whitehouse-Way equation and the heat transfer is calculated by the Annand's equation. The simulated values are compared with measured values of the YANMAR NS90(C) engine and Mitsubishi 4D30 engine, and the following conclusions are drawn. 1. The heat loss by the exhaust gas is well agree with each other in the lower load, but the measured value is greater than the calculated value in the higher load. The maximum error rate is about 15% in the full load. 2. The calculated quantity of heat transfer to the cooling water is greater than the measured value. The maximum error rate is about 11.8%. 3. The mean effective pressure, the fuel consumption, the power and the torque are well agree with each other. The maximum error is occurred in the fuel consumption, and its error rate is about 7%. From the above remarks, it may be concluded that the prediction of the engine performance is possibly by using the developed program, although the program needs to reform by adding the simulation of intake and exhaust process and assumping more reliable mechanical efficiency, volumetric efficiency, preparation rate and combustion rate.

• 터널과지하공간
• /
• 제12권3호
• /
• pp.142-151
• /
• 2002

대전 식품냉동저장창고 파일럿 공동주위의 거리에 따른 비정상상태의 온도 분포를 산정하기 위해 Claesson(2001)의 해석해 및 Dirichlet과 Neuman 내부 경계조건을 갖는 수치모델들을 검토하였다. 온도 강하 단계동안 일정 표면 온도 경계조건에 기초하고 있는 Claesson의 해석해를 활용한 결과, 실제 암반에서의 온도 계측결과를 오차 평균 0.89$^{\circ}C$ 수준으로서 비교적 정확히 예측할 수 있었는데, $0^{\circ}C$근처의 실험실 암석 열물성을 입력하였고 현지 암반 조건을 표현하기 위한 특별한 물성 보정을 하지 않았다. 내부 공동 암반 벽면을 통한 열유속을 갖는 수치해석의 경우, 대류 열전달계수와 공동 내부 온도가 냉각시간에 따라 변화하기 때문에 경계조건을 가하기 어려운 단점을 극복하기 위해 새로운 경계조건 설정 기법을 제안하였다. 그 결과 오차 평균 1.58$^{\circ}C$의 수준으로서 온도 계측치와 부합하였다. 또한 공동 벽면에서 고정 온도 조건을 갖는 수치해와 비교하였다. 마지막으로 Claesson의 해석해 및 다양한 내부 경계조건을 갖는 수치모델을 활용하여, 공동 주변의 온도 분포를 정확히 예측할 수 있는 일련의 해석 단계 프로그램을 제안하였다.

• 대한환경공학회지
• /
• 제31권10호
• /
• pp.933-940
• /
• 2009

본 연구에서는 분무화염의 기초적인 물리현상을 해명하기 위하여 층류 대향류장에 형성된 분무 화염에 2차원 직접 수치계산(Direct numerical simulation, DNS)을 적용하여, 당량비 및 연료종이 분무화염 구조에 미치는 영향에 대하여 관찰하였다. 기상에 대해서는 질량 보존식, 운동량 보존식, 에너지 보존식을 오일리안(Eulerian) 법으로 계산하였으며, 액적에 대해서는 화염중의 모든 개개의 유적을 라그란지안(Lagrangian) 법으로 추적하였다. 액체 연료로는 n-데칸 ($C_{10}H_{22}$)과 n-헵탄($C_7H_{16}$)을 이용하였으며, 연소반응 모델에는 총괄반응식을 이용하였다. 당량비가 증가함에 따라 착화가 빠르며, 고온영역도 넓게 분포하고 있다. 그러나, 최대 온도치는 당량비가 증가함에 따라 한번 증가한 후 감소하는 경향을 나타내고 있다. 당량비가 클수록 최대 온도가 감소하는 것은 분무화염 내부의 군연소 거동에 의한 냉각효과 때문이라고 생각된다. 또한, n-헵탄은 n-데칸과 비교하여 증발속도가 빠르기 때문에 넓은 고온 영역을 형성하지만 최대 온도는 거의 같은 값을 나타내었다.