• 한국정밀공학회지
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• 제21권4호
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• pp.57-63
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• 2004

This paper describes a method for digitizing the compound surfaces which are comprised of several unknown feature shapes such as base surface, and draft wall. From the reverse engineering's point of view, the main step is to digitize or gather three-dimensional points on an object rapidly and precisely. As well known, the non-contact digitizing apparatus using a laser or structured light can rapidly obtain a great bulk of digitized points, while the touch or scanning probe gives higher accuracy by directly contacting its stylus onto the part surface. By combining those two methods, unknown features can be digitized efficiently. The paper proposes a digitizing methodology using the approximated surface model obtained from laser-scanned data, followed by the use of a scanning probe. Each surface boundary curve and the confining area is investigated to select the most suitable digitizing path topology, which is similar to generating NC tool-paths. The methodology was tested with a simple physical model whose shape is comprised of a base surface, draft walls and cavity volumes.

• 오토저널
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• 제10권3호
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• pp.51-59
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• 1988

The wake structure behind a van type vehicle was studied experimentally with a 5-hole yawhead probe. Through an effective calibration method of the 5-hole yawhead probe, the flow properties such as velocity vector, total pressure and static pressure were obtained on two cross sections within the wake. These results combined with the surface flow visualization performed in the previous study, yielded some information about the wake structure. When the model was placed in a stream with zero yaw angle, two counter rotating vortices were observed behind the model which pull down the surface flow on each side of the model. With increasing the yaw angle, the surface flow on the windward side changed to divide the flow in two directions, one flows upward on the upper part and the other flows downward on the lower part of the windward side. Hence a new weak vortex was created on the upper windward side, which resulted 3 vortices within the wake. The size and the strength of the vortices increased with yaw angle.

• 한국기계가공학회지
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• 제6권2호
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• pp.22-27
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• 2007

In this study, the two methods of stiffness measurement(Spring constant) of cantilever were proposed for quantitative measurement in Atomic Force Microscopy(AFM). As the 1st method for the measurement of stiffness, the probe method, which is used in the measurement of the semiconductor mechanical and electrical properties, was applied to the measurement of the cantilever. Experiments by the probe method were performed finding the resistance value of cantilever. As the results, the resistance was measured differently along with the dimension and the thickness of cantilever that determined the stiffness(spring constant) of the lever. As the 2nd method, the vibration characteristics(Dunkerley expression) is used to obtain the stiffness of the complex structure which is combined by AFM cantilever and the standard cantilever. We measured the resonant frequency from the complex structure using the micro stages and stereo microscope. As the results, we confirmed that the vibration characteristics(Dunkerley expression) is effected the micro complex structure of AFM cantilever.

• Journal of Genetic Medicine
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• 제1권1호
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• pp.45-50
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• 1997

Neuroblastoma, a pediatric malignant neoplasm of neural crest origin, has a wide range of clinical virulence. The mechanisms contributing to the development of neuroblastomas are largely unclear, but non-random chromosomal changes identified over the past years suggest the involvement of genetic alterations. Amplification of the human N-myc proto-oncogene is frequently seen either in extrachromosomal double minutes or in homogeneously staining regions (HSRs) of aggressively growing neuroblastomas. N-myc maps to chromosome 2 band 24, but HSR have never been observed at this band, suggesting transposition of N-myc during amplification. We have constructed and analyzed the region-specific painting probe for HSR in neuroblastoma IMR-32 to determine the derivative chromosomes. Microdissection was performed on HSR using an inverted microscope with the help of microglass needles and an micromanipulator. We pretreated the microdissected fragments with Topoisomerase I which catalyzes the relaxation of supercolled DNA, and performed two initial rounds of DNA synthesis with T7 DNA polymerase followed by conventional PCR to enable the reliable preparation of Fluorescent in situ hybridization probe from a single microdissected chromosome. With this method, it was possible to construct the region-specific painting probe for HSR. The probe hybridized specifically to the HSRs of IMR-32, and to 2p24, 2p13 of normal chromosome. Our results suggest there was coamplification of N-myc together with DNA of the chromosome 2p24 and 2p13. Moreover, the fluorescent signals for the amplified chromosomal regions in IMR-32 cells were also easily recognized at a Thus this painting probe can be applied to detect the similar amplification of N-myc in neuroblastoma tissue, and the probe pool for HSR may be used to identify the cancer-relevant genes.

• 한국ITS학회 논문지
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• 제18권5호
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• pp.183-193
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• 2019

본 연구에서는 전/후방 차량 검지가 가능한 차량센서를 탑재한 프로브 차량으로 수집한 주행 궤적을 이용하여 연속웨이블릿변환 기반 충격파 검지 및 소멸 시점 예측 방법을 제안하였다. 제안된 방법의 효과성 분석을 위하여 충격파 소멸점 간 거리오차와 충격파 소멸점 시간-위치 오차를 평가 지표로 제시하였고, 교통혼잡 수준, 속도 감소 현상 지속시간, 프로브 차량의 비율 등을 실험요인으로 하는 미시적 시뮬레이션 기반의 실험을 통하여 제안된 방법의 개념을 검증하였다. 그 결과, 두 가지 평가 지표 모두 교통혼잡 수준 및 속도 감소 지속시간에 크게 민감하지 않음을 보임으로서, 본 연구에서 제안하는 방법이 임의의 위치와 시간 동안 발생하는 속도 감소 현상으로 인한 충격파를 검지하고 그 소멸시점을 예측하는데 효과가 있음을 보였다. 그리고 그 정확도는 전체 차량 중 프로브 차량의 비율에 많은 영향을 받는 것으로 나타났다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3657-3661
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• 2012

HBV infection is contagious and may be transmitted vertically or horizontally by blood products and body secretions. Over 50% of Iranian carriers have contracted the infection prenatally, making this the most likely route of transmission of HBV in Iran. This study assesses the resistance to Lamivudine in patients with chronic hepatitis B infection using a new ZNA probe Real Time PCR method. To evaluate the effectiveness of Lamivudine therapy for chronic hepatitis B infection, a study was conducted on 70 patients (63 men and 7women), who had received the drug first line. All patients were tested for the presence of HBsAg and HBeAg, the serum ALT level and the HBV DNA load before and after treatment. In all samples resistance to Lamivudine was tested with the ZNA Probe. Our results showed that ZNA Probe Real Time PCR method could detect wild type,YMDD, and its mutants, tyrosine-isoleucine-aspartate-aspartate and tyrosine-valine-aspartate-Aspartate. Among an estimated seventy patients with chronic hepatitis B infection, 18 (25.7%) were resistant to lamivudine. Only one patient was negative for presence of HBS-Ag (5.6%) and two patients were negative for HBe-Ag (11.1%). Real-time PCR with Zip nucleic acid probes is a sensitive, specific and rapid detection method for mutations in the YMDD motif, which will be essential for monitoring patients undergoing Lamivudine antiviral therapy.

• ETRI Journal
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• 제17권2호
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• pp.11-19
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• 1995

The plasma densities generated from a semiconductor bridge (SCB) device employing a capacitor discharge firing set have been measured by a novel diagnostic technique employing a microwave resonator probe. The spatial resolution of the probe is comparable to the separation between the two wires of the transmission lines (${\approx}$3 mm). This method is superior to Langmuir probes in this application because Langmuir probe measurements are affected by sheath effects, small bridge area, and unknown fraction of multiple ions. Measured electron densities are related to the land material and input energy. Although electron densities in the plasma generated by aluminum or tungsten-land SCB devices show a general tendency to increase steadily with power, at the higher energies, the electron densities generated from tungsten-land SCB devices are found to remain constant.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2002년도 춘계학술대회 논문집
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• pp.546-550
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• 2002

This paper proposes an error compensation method that improves accuracy with geometry information of injection molding parts. Geometric information can give an improved accuracy in reverse engineering. Measuring data can not lead to get accurate geometric model, including errors of physical parts and measuring machines. Measuring data include errors which can be classified into two types. One is molding error in product, the other is measuring error. Measuring error includes optical error of laser scanner, deformation by probe forces of CMM and machine error. It is important to compensate these in reverse engineering. Least square method(LSM) provides the cloud data with a geometry compensation, improving accuracy of geometry. Also, the functional shape of a part and design concept can be reconstructed by error compensation using geometry information.

• 한국공작기계학회논문집
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• 제11권1호
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• pp.83-90
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• 2002

As high-precision parts are needed with manufacturing techniques improved. the demand of high-precision machine tools has been increasing They are made to developed the precision measuring skill to maintenance the accuracy of themselves as a matter or course We one paper measured straightness and orthogonality of the square to verify that it is possible for CT(circular test) method by 2-dimensional probe and the square to measure orthogonality Furthemore we compared the result of the study with the computer simulation's to prove its possibility and made an improvement of measuring method.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2000년도 춘계학술대회논문집B
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• pp.541-547
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• 2000

Experiments were done for the comparison of performance and flow characteristics between a two stage axial flow fan and a counter-rotating axial flow fm. The fan performance curves were obtained by the Korean Standard Testing Methods for Turbo Fans and Blowers (KS B 6311). The fan flow characteristics were measured using a five-hole probe by the non-nulling method. Each stage of the two stage axial flow fan used for the present study has an eight bladed rotor and thirteen stator blades. The front and the rear rotor of the counter-rotating axial flow fan have eight blades each and are driven by coaxial counter rotating shafts through a gear box located between the rear rotor and the electric motor. Both of the two axial fan configurations use identical rotor blades and the same operating conditions for the one-to-one comparison of the two. Performance characteristics of the two configurations were obtained and compared by varying the blade setting angles and axial gaps between the blade rows. The passage flow fields between the hub and tip of the fans were measured and analyzed for the particular operating conditions of peak efficiency, minimum and maximum pressure coefficients.