• KSBB Journal
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• v.15 no.6
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• pp.556-559
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• 2000

Cyclodextrin Glucanotransferase(EC 2.4.1.19 : 1,4-${\alpha}$-glucano) transferase, cyclizing; CGTase) can be separated and recovered in an aqueous two-phase system composed of poly(ethylene glycol)(PEG)/dextran and PEG/salt. In an aqueous two-phase system consisting of PEG 35000 (5%) and dextran T2000 (7%), all cell and debris were collected at the interphase. CGTase partitioned to the denser dextran phase at an yield of 83.4%. On the other hand, in an aqueous two-phase system consisting of PEG 35000 (10%) and sodium phosphate (15%), CGTase partitioned to the denser salt phase at an yield of 95.5%. In order to recover CGTase using an aqueous two-phase system, the PEG/salt system proved to be more efficient than the PEG/dextran system in terms of yield and cost.

• Journal of the Korean Society for Precision Engineering
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• v.15 no.3
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• pp.107-118
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• 1998

Two-phase neuro-system identification method is presented. The 1$^{st}$-phase identification uses conventional neural network mapping for modeling an input-output system. The 2$^{nd}$ -phase modeling is also performed sequentially using the 1$^{st}$-phase modeling errors. In the 2$^{nd}$ a phase modeling, newly generated input signals, which are obtained by summing the 1st-phase modeling error and artificially generated uniform series, are utilized as system's I-O mapping elements. The 1$^{st}$-phase identification is interpreted as a “Real Model” system identification because it uses system's real data(i.e., observations and control inputs) while the 2$^{nd}$ -phase identification as a “Artificial Model” identification because of using artificial data. Experimental results are given to verify that the two-phase neuro-system identification could reduce the overall modeling errors.rrors.

• Microbiology and Biotechnology Letters
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• v.20 no.1
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• pp.61-67
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• 1992

The synthesis of N-benzyloxycarbonyl-L-aspartyl-L-phenylalanine methyl ester(ZAPM), a precursor of aspartame, from N-benzyloxycarbonyl-L-aspartic acid(Z-Asp) and L-phenylalanine methyl ester hydrochloride(L-PM-HCl) was investigated in ethylacetate-MES buffer two-phase system using thermolysin. In organic two-phase system, the degree of spontaneous hydrolysis of L-PM. HCl was significantly reduced with increasing the volume ratio of organic to aqueous phase. Stability of thermolysin in organic two-phase system was found to be higher than that in MES buffer solution. More than 90% of initial enzyme activity was maintained after 10 days of incubation in case that the volume of organic phase was equal to that of buffer phase, while the half life of thermolysin was about 2 days in aqueous buffer solution. The results of partitioning of substrates and product in organic two-phase system showed that the difference in partition coefficients between substrates and product was maximum at pH 5.5. The optimal pH for 2-APM synthesis in organic two-phase system was found to be 5.5-5.8, which is consistent with the value expected from the partition experiments. As the concentration of substrates was increased the conversion yield of Z-APM was increased with concomitant reduction of L-PMqHC1 hydrolysis. In case that the concentration of L-PM-HCl and Z-Asp were 160 mM and 80 mM respectively, the conversion yield of Z-APM reached 90% after 28 hrs of reaction. The yield obtained at different volume ratio of organic phase compares well with the predicted equilibrium constant in biphasic system.

• Nuclear Engineering and Technology
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• v.56 no.1
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• pp.19-33
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• 2024

In nuclear thermal-hydraulic system codes, most correlations used for vertical pipes, under downward two-phase flow, have been developed considering small pipes or pool systems. This suggests that there could be uncertainties in applying the correlations to accident scenarios involving large vertical pipes owing to the difference in the characteristics of two-phase flows, or flow conditions, between large and small pipes. In this study, we modified the Multi-dimensional Analysis of Reactor Safety KINS Standard (MARS-KS) code using correlations, such as the drift-flux model and two-phase multiplier, developed in a plant-scale air-inflow experiment conducted for a pipe of diameter 600 mm under downward two-phase flow. The results were then analyzed and compared with those based on previous correlations developed for small pipes and pool conditions. The modified code indicated a good estimation performance in two plant-scale experiments with large pipes. For the siphon-breaking experiment, the maximum errors in water flow for modified and original codes were 2.2% and 30.3%, respectively. For the air-inflow accident experiment, the original code could not predict the trend of frictional pressure gradient in two-phase flow as / increased, while the modified MARS-KS code showed a good estimation performance of the gradient with maximum error of 3.5%.

• Nuclear Engineering and Technology
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• v.53 no.12
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• pp.3892-3901
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• 2021

The pressure drop of a moisture separator in a steam generator is the important design parameter to ensure the successful performance of a nuclear power plant. The moisture separators have a wide range of operating conditions based on the arrangement of them. The prediction of the pressure drop in a moisture separator is challenging due to the complexity of the multi-dimensional two-phase vortex flow. In this study, the moisture separator test facility using the air/water two-phase flow was used to predict the pressure drop of a moisture separator in a Korean OPR-1000 reactor. The prototypical steam/water two-phase flow conditions in a steam generator were simulated as air/water two-phase flow conditions by preserving the centrifugal force and vapor quality. A series of experiments were carried out to investigate the effect of hydraulic characteristics such as the quality and liquid mass flux on the two-phase pressure drop. A new prediction model based on the scaling law was suggested and validated experimentally using the full and half scale of separators. The suggested prediction model showed good agreement with the steam/water experimental results, and it can be extended to predict the steam/water two-phase pressure drop for moisture separators.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.36 no.3
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• pp.171-178
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• 1987

A study of two phase chopper system with four separate groups of DC motors in powering. A novel two phase chopper system with four separate groups of DC motors which applies the principles of two phase chopper with two separate groups of DC motors is dealt with this article. The main circuit consists of eight sets of chopping parts, four diodes and four separate groups of DC motors. Four groups of DC motors are driven through the series and parallel connections to each other in accordance with the operating conditions of the choppers. Although the proposed chopper circuit requires more circuit elements than the conventional two phase chopper system with combined output or two phase chopper system with two separate groups of DC motors, it has the following advantages` (1). It is possible to drive twice as much motors as conventional system does, using esisting receiving-and equipments and motors. (2). It is possible to control load voltage continuously from 0 to source voltage by changing time-ratio from 0 to 1. (3). Load current division becomes equalized. Therefore it is possible to drive not only series motors but also shunt and separately exited motors. (4). When smoothing reactor L is small, harmonic components of the proposed circuit is not so large. Therefore, the value of L can be determined from viewpoints of allowable value of ripple-ratio and current unbalance factor.

• The Transactions of the Korean Institute of Power Electronics
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• v.12 no.4
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• pp.310-317
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• 2007

In this paper, the system equation for the balanced two-phase induction motor is derived and the characteristics for speed control is also analyzed in the region of constant torque and constant power. The modified vector control theory is applied to two-phase motor drive system. The speed of two-phase motor drive can be controlled precisely by the modified indirect vector control theory. The modified vector control theory is simpler comparing to the conventional vector control because of the simpler axis transformation. The computer simulations and the experimental results presented to confirm the vector control for two-phase inverter fed two phase induction motor system.

• Journal of Microbiology and Biotechnology
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• v.2 no.2
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• pp.135-140
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• 1992

The partitioning of recombinant human interleukin-2(rhII-2) in PEG 8000-dextran 38800 aqueous two-phase system has been investigated using three different sources of rhIL-2. In the case of pure rhIL-2, the solubility in a PEG-dextran two-phase system was low and most of rhIL-2 was partitioned into the bottom phase. For the recovery of rhIL-2 from insoluble protein aggregates, the inclusion bodies of recombinant E. coli were solubilized by the treatment with sodium dodecyl sulfate (SDS). The addition of SDS significantly enhanced not only the solubility of rhIL-2 but also the partitioning of rhIL-2 to the top phase. When the ratio of SDS to rhIL-2 was 2.0, the partition coefficient(K) and the recovery yield(Y) at the top phase were 4.5 and 88%, respectively, at pH 6.8. In order to reduce the recovery steps further, SDS was directly added to the intact recombinant E. coli cells and then partitioned into the PEG/dextran aqueous two-phase system. The observed partition coefficient ($K{\cong{3.0$) and recovery yield ($Y{\geq}80%$ )of this method were comparable to the rhIL-2 recovery from insoluble protein aggregates. The results obtained in this work indicate that PEG-dextran two-phase partitioning might provide a simple way for the recovery and partial purification of recombinant proteins which are produced as inclusion bodies.

• The Transactions of the Korean Institute of Power Electronics
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• v.26 no.4
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• pp.256-262
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• 2021

Six-phase motors can be used in industrial applications, such as an electric vehicle, due to their high reliability and low current magnitude per phase. An asymmetrical PMSM with two sets of three-phase windings is a commonly used structure for six-phase motors, with each winding set demonstrating a phase difference of 30°. Although the asymmetrical PMSM presents low torque ripples, its dynamic torque response deteriorates due to coupled components in the two three-phase windings. The decoupled VSD control is applied to eliminate the coupling effect. Load ratio control of two inverters for the six-phase PMSM is proposed in this study. DQ currents are controlled on the basis of two synchronous reference frames, and the six-phase drive system can be changed to a three-phase drive system when one inverter presents fault conditions. The operation and effectiveness of the proposed algorithm is verified through simulation and experiments. The six-phase drive system is transferred to a three-phase drive system by changing the current reference of the second DQ reference frame. Moreover, control of both torque and speed exhibits satisfactory performance before and after the mode change.

• Microbiology and Biotechnology Letters
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• v.22 no.5
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• pp.531-537
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• 1994

This study was undertaken with objective of optimizing the conditions of fermentation in an aqueous two-phase system which is composed of polyethylene glycol (PEG) 20000 and crude dextran (Dx). The data were obtained and analyzed using the Box-Wilson's experimental design protocol and the response surface methodology. To reach this end a multilinear polynomial regres- sion model was developed, which can be utilized for the purpose of optimizing the extractive fermentation. Optimum conditions for batch fermentation with aqueous two phase system were found to be at 4.2~5.4% PEG/3.2~4.2% Dx range. The composition of the center was 4.8% PEG/ 3.6% Dx. Optimum operating conditions for initial sugar concentration and fermentation time were approximately 160 g/l, and 21~22 hr, respectively. Fermentation in the aqueous two phase system composed of 5% PEG/4% Dx showed increase of 23% in ethanol concentration, of 9.5% in ethanol yield, and of 19% in ethanol productivity as compared to the case of fermentation of neat Jerusalem artichoke juice.