• Macromolecular Research
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• 제15권5호
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• pp.449-458
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• 2007

The aims of this study were to investigate the intercalation of polymer chains with organoclays and improve the thermo-mechanical properties of poly(butylene terephthalate) (PBT) hybrids by comparing PBT hybrids synthesized using two different organoclays. The organoclays; dodecyltriphenylphosphonium-montmorillonite ($C_{12}PPh-MMT$) and dodecyltriphenylphosphonium-mica ($C_{12}PPh-Mica$), were used to fabricate the PBT hybrid fibers. Variations in the properties of the hybrid fibers with the organoclays within the polymer matrix, as well as the draw ratio (DR), are discussed. The thermo-mechanical properties and morphologies of the PBT hybrid fibers were characterized using differential scanning calorimetry, thermogravimetric analysis, wide-angle X-ray diffraction, electron microscopy and mechanical tensile properties analysis. The nanostructures of the hybrid fibers were determined using both scanning and transmission electron microscopies, which showed some of the clay layers to be well dispersed within the matrix polymer, although some clustered or agglomerated particles were also detected. The thermal properties of the hybrid fibers were found to be better than those of the pure PBT fibers at a DR = 1. The tensile mechanical properties of the $C_{12}PPh-MMT$ hybrid fibers were found to worsen with increasing DR. However, the initial moduli of the $C_{12}PPh-Mica$ hybrid fibers were found to slightly increase on increasing the DR from 1 to 18.

• Industrial Engineering and Management Systems
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• 제14권1호
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• pp.22-31
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• 2015

In this paper, a two-stage hybrid flow shop problem is considered. Specifically, there exist identical parallel machines at stage 1 and two dedicated machines at stage 2, and the objective of the problem is to minimize makespan. After being processed by any machine at stage 1, a job must be processed by a specific machine at stage 2 depending on the job type, and one type of jobs can have different processing times on each machine. First, we introduce the problem and establish complexity of several variations of the problem. For some special cases, we develop optimal polynomial time solution procedures. Then, we establish some simple lower bounds for the problem. In order to solve this NP-hard problem, three heuristics based on simple rules such as the Johnson's rule and the LPT (Longest Processing Time first) rule are developed. For each of the heuristics, we provide some theoretical analysis and find some worst case bound on relative error. Finally, we empirically evaluate the heuristics.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 ITC-CSCC -2
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• pp.1096-1099
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• 2000

A method to analyze the high speed inter-connects that are composed of frequency dependent lossy distributed lines is presented. Network modeling of hybrid systems is implemented by using the modified nodal admittance matrix in the Laplace transformation domain. The network response is computed by different two methods. One method Is the asymptotic waveform evaluation (AWE) method and other is numerical Laplace inversion method. The merits and demerits of two methods are discussed by applying to several concrete illustrative networks.

• BMB Reports
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• 제37권1호
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• pp.45-52
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• 2004

The goal of interaction proteomics that studies the protein-protein interactions of all expressed proteins is to understand biological processes that are strictly regulated by these interactions. The availability of entire genome sequences of many organisms and high-throughput analysis tools has led scientists to study the entire proteome (Pandey and Mann, 2000). There are various high-throughput methods for detecting protein interactions such as yeast two-hybrid approach and mass spectrometry to produce vast amounts of data that can be utilized to decipher protein functions in complicated biological networks. In this review, we discuss recent developments in analytical methods for large-scale protein interactions and the future direction of interaction proteomics.

• BMB Reports
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• 제34권1호
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• pp.15-20
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• 2001

One of the innate immune reactions in invertebrates is the pro-phenoloxidase (pro-PO) activation system that is involved in the generation of superoxide, melanin synthesis, and the subsequent sequestration of foreign matter entering the hemocoel of the invertebrates. However, the molecular mechanism of this biological reaction is still obscure. To expand our understanding of the biological roles of the pro-PO activation system in invertebrates, we performed a yeast two-hybrid screening by using three regions of pro-PO as bait and a yeast two-hybrid cDNA library from Tenebrio molitor larvae as prey We isolated a novel partial cDNA clone that encodes a glycine-rich protein that interacted with the active phenoloxidase (termed phenoloxidase interacting protein, POIP). POIP consists of two domains: One is an N-terminal unique domain and the other is a C-terminal glycine-rich domain. The C-terminal glycine-rich domain showed sequential homology with those of insect antifungal proteins. Also, the yeast two-hybrid screen in a reverse orientation (using POIP as bait) yielded PO, suggesting that the PO-POIP interaction is specific. By using a 315 bP PCR fragment of the N-terminal unique region of POIP, we cloned the full-length cDNA of POIP from the Tenebruo cDNA library constructed by using E. coli injected larvae. The interaction analysis between PO, and a truncated fragment lacking the N-terminal unique region of POIP, indicated that the N-terminal unique region is necessary for interaction between PO and POIP. The expression level of the POIP mRNA is increased by bacterial injection into T. molitor larvae. This suggests that POIP might be engaged in the humoral defense reaction.

• 한국정밀공학회지
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• 제25권12호
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• pp.107-116
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• 2008

The excitation force of a powertrain is one of major sources for the interior noise of a vehicle. This paper presents a novel approach to predict the interior noise caused by the vibration of the power rain by using the hybrid TPA (transfer path analysis) method. Although the traditional transfer path analysis (TPA) is useful for the identification of powertrain noise sources, it is difficult to modify the structure of a powertrain by using the experimental method for the reduction of vibration and noise. In order to solve this problem, the vibration of the power rain in a vehicle is numerically analyzed by using the finite element method (FEM). The vibration of the other parts in a vehicle is investigated by using the experimental method based on vibrato-acoustic transfer function (VATF) analysis. These two methods are combined for the prediction of interior noise caused by a power rain. Throughout this research, two papers are presented. This paper presents a simulation of the excitation force of the power rain exciting the vehicle body based on numerical simulation. The other paper presents a prediction of interior noise based on the hybrid TPA, which uses the VATF of the car body and the excitation force predicted in this paper.

• Wind and Structures
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• 제9권4호
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• pp.331-344
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• 2006

The cable-stayed-suspension hybrid bridge is a cooperative system developed from the traditional cable-stayed and suspension bridges, and takes some advantages of the two bridge systems. It is also becoming a competitive design alternative for some long and super long-span bridges. But due to its great flexibility, the flutter stability plays an important role in the design and construction of this bridge system. Considering the geometric nonlinearity of bridge structures and the effects of nonlinear wind-structure interaction, method and its solution procedure of three-dimensional nonlinear flutter stability analysis are firstly presented. Parametric analyses on the flutter stability of a cable-stayed-suspension hybrid bridge with main span of 1400 meters are then conducted by nonlinear flutter stability analysis, some design parameters that significantly influence the flutter stability are pointed out, and the favorable structural system of the bridge is also discussed based on the wind stability.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.613-619
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• 2005

Indigenous Yakutian cattle' adaptation to the hardest subarctic conditions makes them a valuable genetic resource for cattle breeding in the Siberian area. Since early last century, crossbreeding between native Yakutian cattle and imported Simmental and Kholmogory breeds has been widely adopted. In this study, variations at 22 polymorphic microsatellite loci in 5 populations of Yakutian, Kholmogory, Simmental, Yakutian-Kholmogory and Yakutian-Simmental cattle were analysed to estimate the genetic contribution of Yakutian cattle to the two hybrid populations. Three statistical approaches were used: the weighted least-squares (WLS) method which considers all allele frequencies; a recently developed implementation of a Markov chain Monte Carlo (MCMC) method called likelihood-based estimation of admixture (LEA); and a model-based Bayesian admixture analysis method (STRUCTURE). At population-level admixture analyses, the estimate based on the LEA was consistent with that obtained by the WLS method. Both methods showed that the genetic contribution of the indigenous Yakutian cattle in Yakutian-Kholmogory was small (9.6% by the LEA and 14.2% by the WLS method). In the Yakutian-Simmental population, the genetic contribution of the indigenous Yakutian cattle was considerably higher (62.8% by the LEA and 56.9% by the WLS method). Individual-level admixture analyses using STRUCTURE proved to be more informative than the multidimensional scaling analysis (MDSA) based on individual-based genetic distances. Of the 9 Yakutian-Simmental animals studied, 8 showed admixed origin, whereas of the 14 studied Yakutian-Kholmogory animals only 2 showed Yakutian ancestry (>5%). The mean posterior distributions of individual admixture coefficient (q) varied greatly among the samples in both hybrid populations. This study revealed a minor existing contribution of the Yakutian cattle in the Yakutian-Kholmogory hybrid population, but in the Yakutian-Simmental hybrid population, a major genetic contribution of the Yakutian cattle was seen. The results reflect the different crossbreeding patterns used in the development of the two hybrid populations. Additionally, molecular evidence for differences among individual admixture proportions was seen in both hybrid populations, resulting from the stochastic process in crossing over generations.

• Fisheries and Aquatic Sciences
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• 제21권5호
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• pp.11.1-11.8
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• 2018

Interspecific hybridization experiments were conducted between the common seahorse Hippocampus kuda (male) and the slender seahorse H. reidi (female) during artificial rearing to develop a new aquarium fish with unique polyandrous mating. Molecular analysis via mitochondrial DNA (mtDNA) cytochrome b and nuclear DNA (ncDNA) ribosomal protein S7 gene supported the hybridization between the two species, and the hybrid also showed morphological characteristics of both species. Juveniles of H. kuda have dense melanophores on the whole body or only on the trunk and tail, whereas juveniles of H. reidi have thin melanophores on the whole body or present in stripes only along their prominent trunk and tail rings. However, all the hybrid juveniles had dense melanophores only on the tail, with the striped trunk rings, thus showing an intermediate pattern, and these patterns were limited to the fairly early stage of development (1-10 days old). In contrast, the two eye spines in the hybrid were apparent after 9 days old, which were not inherited from H. kuda (one eye spine), but from H. reidi (two eye spines). According to LOESS (local regression) analysis, the growth rate increased between 20 and 25 days, and the hybrids grew faster than H. kuda when they entered the explosive second phase of growth between 25 and 45 days for all the seahorses. This study highlights the hybridization between H. kuda and H. reidi may contribute to the improved taxonomic information of young seahorses.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2008년도 춘계종합학술대회 A
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• pp.129-132
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• 2008

Six-port 위상 상관기는 한 개의 divider와 세 개의 coupler로 구성되어 진다. 일반적인 wilkinson power divider와 $90^{\circ}$ hybrid coupler를 결합하여 제작시 10% 미만의 대역특성을 갖는다. 본 논문에서는 대역폭이 33%인 2 section power divider와 15%인 external matching $90^{\circ}$ hybrid coupler 세 개를 이용하여 six-port 위상 상관기를 시뮬레이션 후 제작하였다. ADS를 이용한 시뮬레이션 결과 중심 주파수 2.5GHz에서 RF port, LO port 모두 15%의 대역폭을 얻을 수 있었다. 이러한 시뮬레이션 결과를 바탕으로 실제 제작한 six-port 위상 상관기는 손실이 증가 하였으나 비슷한 대역폭(600MHz)을 얻을 수 있었다. 또 이 구간에서의 위상 오차는 약 $5^{\circ}$로 양호한 특성을 얻었다.