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Classification of behavioral signs of the mares for prediction of the pre-foaling period

  • Jung, Youngwook;Jung, Heejun;Jang, Yongseok;Yoon, Duhak;Yoon, Minjung
    • Journal of Animal Reproduction and Biotechnology
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    • v.36 no.2
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    • pp.99-105
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    • 2021
  • In horse management, the alarm system with sensors in the foaling period enables the breeder can appropriately prepare the time of the parturition. It is important to prevent losses by unpredictable parturition because there are several high risks such as dystocia and the death of foals and mares during foaling. However, unlike analysis in the alarm system that detects specific motions has been widely performed, analysis of classification following specific behavior patterns or number needs to be more organized. Thus, the objective of this study is to classify signs of the specific behaviors of the mares for the prediction of pre-foaling behaviors. Five Thoroughbred mares (9-20 yrs) were randomly selected for observation of the pre-foaling behaviors. The behaviors were monitored for 90 min that was divided into three different periods as 1) from -90 to -60 min, 2) from -60 to -30 min, 3) from -30 min to the time for the discharge of the amniotic fluid, respectively. The behaviors were divided into two different categories as state and frequent behaviors and each specific behavioral pattern for classification was individually described. In the state behaviors, the number of mares in the standing of the foaling group (3.17 ± 0.18b) at period 3 was significantly higher than the control group (1.67 ± 0.46a). In contrast, the number of the mares in the eating of the foaling group (1.17 ± 0.34b) at period 3 was significantly lower than the control group (3.33 ± 0.46a). In the frequent behaviors, the weaving of the foaling group was significantly higher than the control group, and looking at the belly of the foaling group was significantly lower than the control group. In period 2, defecation, weaving, and lowering the head of the foaling group were significantly higher than the control group, respectively. In period 3, sitting down and standing up, pawing, weaving, and lowering the head in the foaling group were also significantly higher than the control group. In conclusion, the behavior is significantly different in foaling periods, and the prediction of foaling may be feasible by the detection of the pre-foaling behaviors in the mares.

Evaluation of liquid and powdered forms of polyclonal antibody preparation against Streptococcus bovis and Fusobacterium necrophorum in cattle adapted or not adapted to highly fermentable carbohydrate diets

  • Cassiano, Eduardo Cuellar Orlandi;Perna, Flavio Junior;Barros, Tarley Araujo;Marino, Carolina Tobias;Pacheco, Rodrigo Dias Lauritano;Ferreira, Fernanda Altieri;Millen, Danilo Domingues;Martins, Mauricio Furlan;Pugine, Silvana Marina Piccoli;de Melo, Mariza Pires;Beauchemin, Karen Ann;Meyer, Paula Marques;Arrigoni, Mario de Beni;Rodrigues, Paulo Henrique Mazza
    • Animal Bioscience
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    • v.34 no.1
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    • pp.74-84
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    • 2021
  • Objective: Feed additives that modify rumen fermentation can be used to prevent metabolic disturbances such as acidosis and optimize beef cattle production. The study evaluated the effects of liquid and powdered forms of polyclonal antibody preparation (PAP) against Streptococcus bovis and Fusobacterium necrophorum on rumen fermentation parameters in ruminally cannulated non-lactating dairy cows that were adapted or unadapted to a high concentrate diet. Methods: A double 3×3 Latin square design was used with three PAP treatments (control, powdered, and liquid PAP) and two adaptation protocols (adapted, unadapted; applied to the square). Adapted animals were transitioned for 2 weeks from an all-forage to an 80% concentrate diet, while unadapted animals were switched abruptly. Results: Interactions between sampling time and adaptation were observed; 12 h after feeding, the adapted group had lower ruminal pH and greater total short chain fatty acid concentrations than the unadapted group, while the opposite was observed after 24 h. Acetate:propionate ratio, molar proportion of butyrate and ammonia nitrogen concentration were generally greater in adapted than unadapted cattle up to 36 h after feeding. Adaptation promoted 3.5 times the number of Entodinium protozoa but copy numbers of Streptococcus bovis and Fibrobacter succinogens genes in rumen fluid were not affected. However, neither liquid nor powdered forms of PAP altered rumen acidosis variables in adapted or unadapted animals. Conclusion: Adaptation of cattle to highly fermentable carbohydrate diets promoted a more stable ruminal environment, but PAP was not effective in this study in which no animal experienced acute or sub-acute rumen acidosis.

Understanding and Applicability of Identity-in-practice Theory (실행 과정에서의 정체성 이론의 이해와 적용 방안)

  • Kim, Jong-Uk;Kim, Chan-Jong
    • Journal of The Korean Association For Science Education
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    • v.41 no.3
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    • pp.267-281
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    • 2021
  • The shift in the perspective on learning as participation draws attention to learners' identity work. This manuscript foregrounds the ongoing and fluid processes of identity, and focuses on the structure surrounding learners where identity work occurs and the expression of individual agency. From the perspective of learning as participation, we introduce Holland et al. (1998) identity-in-practice theory, as the theoretical lens that dialectically describes structure, agency and identity. The concepts representing this theory are 'figured world', 'positionality' and 'authoring self'. The figured world is a web of meaning encompassing the continuous construct/reconstruct through human actions and also exerts as the social force that shapes human actions. 'Positionality' on the other hand, means an understanding of one's social position in the world. The notion of 'authoring self', is a dimension that synthesizes the above two concepts, which describes the improvisation of one's self through the orchestration and collaboration of various social voices. Next, we analyzed and described various cases of this theory that we found within the scope of science education. However, due to the nature of this theory, which takes a sociocultural approach, international research studies cannot be simply applied to the Korean context. Therefore, we sought to expand the applicability of this theory as a methodological tool, by discussing the directions in which this theory can be used in a domestic context. It is intended that the introduction of this theory, which emphasizes multi-layered, continuous and cumulative identity work, will be used as a resource to further expand the horizon of science education reform that is required for the times.

Ruminal pH pattern, fermentation characteristics and related bacteria in response to dietary live yeast (Saccharomyces cerevisiae) supplementation in beef cattle

  • Zhang, Xiangfei;Dong, Xianwen;Wanapat, Metha;Shah, Ali Mujtaba;Luo, Xiaolin;Peng, Quanhui;Kang, Kun;Hu, Rui;Guan, Jiuqiang;Wang, Zhisheng
    • Animal Bioscience
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    • v.35 no.2
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    • pp.184-195
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    • 2022
  • Objective: In this study we aimed to evaluate the effect of dietary live yeast supplementation on ruminal pH pattern, fermentation characteristics and associated bacteria in beef cattle. Methods: This work comprised of in vitro and in vivo experiments. In vitro fermentation was conducted by incubating 0%, 0.05%, 0.075%, 0.1%, 0.125%, and 0.15% active dried yeast (Saccharomyces cerevisiae, ADY) with total mixed ration substrate to determine its dose effect. According to in vitro results, 0.1% ADY inclusion level was assigned in in vivo study for continuously monitoring ruminal fermentation characteristics and microbes. Six ruminally cannulated steers were randomly assigned to 2 treatments (Control and ADY supplementation) as two-period crossover design (30-day). Blood samples were harvested before-feeding and rumen fluid was sampled at 0, 3, 6, 9, and 12 h post-feeding on 30 d. Results: After 24 h in vitro fermentation, pH and gas production were increased at 0.1% ADY where ammonia nitrogen and microbial crude protein also displayed lowest and peak values, respectively. Acetate, butyrate and total volatile fatty acids concentrations heightened with increasing ADY doses and plateaued at high levels, while acetate to propionate ratio was decreased accordingly. In in vivo study, ruminal pH was increased with ADY supplementation that also elevated acetate and propionate. Conversely, ADY reduced lactate level by dampening Streptococcus bovis and inducing greater Selenomonas ruminantium and Megasphaera elsdenii populations involved in lactate utilization. The serum urea nitrogen decreased, whereas glucose, albumin and total protein concentrations were increased with ADY supplementation. Conclusion: The results demonstrated dietary ADY improved ruminal fermentation dose-dependently. The ruminal lactate reduction through modification of lactate metabolic bacteria could be an important reason for rumen pH stabilization induced by ADY. ADY supplementation offered a complementary probiotics strategy in improving gluconeogenesis and nitrogen metabolism of beef cattle, potentially resulted from optimized rumen pH and fermentation.

Effects of Additional Levels of Phyllostachys bambusoides on Ruminal Fermentation Characteristics and Methane Emission in in vitro (왕대의 첨가수준이 반추위 in vitro 발효성상과 메탄 발생량에 미치는 영향)

  • Jo, Seong-Uk;Lee, Shin-Ja;Lee, Ye-Jun;Kim, Hyun-Sang;Eom, Jun-Sik;Choi, You-Young;Bae, Eun-Ji;Lee, Sung-Sill
    • Korean Journal of Organic Agriculture
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    • v.29 no.2
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    • pp.241-256
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    • 2021
  • The current study was to evaluate the antioxidant activity of Phyllostachys bambusoides (PHB) as a feed additives and investigate whether its antioxidant activity could be helpful for increasing rumen fermentation characteristics and methane reduction. The antioxidant activity results showed that total polyphenols and flavonoids contents were 43.54 ± 8.68 mg CE/g and 17.13 ± 0.45 mg QE/g, respectively, and the IC50 values for 1,1-diphenyl-2-prcrylhydrazyl (DPPH) and 2,2'-azino-bis (3- ethylbenzthiazoline-6- sulphonic acid) (ABTS) radical scavenging activity were 163.13 ± 19.25 ㎍/mL and 97.07 ± 4.46 ㎍/mL, respectively. Two heads of cannulated Hanwoo (450 ± 30 kg), consuming timothy hay and a commercial concentrate (60:40, w/w) twice daily (at 09:00 and 17:30) at 2% of body weight, with free access to water and a mineral block, were used as rumen fluid donors. An in vitro incubation experiment was performed after 6, 12, 24, 48, and 72 hr with PHB added at concentration of 2, 4, and 6% of timothy hay basis. Total gas emission decreased as the amount of PHB addition increased at 6 and 24 hr of incubation. However, PHB addition did not affect total volatile fatty acid production, and methane and carbon dioxide emission also decreased as the amount of addition increased at 48 hr of incubation. Therefore, PHB was expected to be used as methane reducing additives in the ruminants.

Multi-fidelity uncertainty quantification of high Reynolds number turbulent flow around a rectangular 5:1 Cylinder

  • Sakuma, Mayu;Pepper, Nick;Warnakulasuriya, Suneth;Montomoli, Francesco;Wuch-ner, Roland;Bletzinger, Kai-Uwe
    • Wind and Structures
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    • v.34 no.1
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    • pp.127-136
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    • 2022
  • In this work a multi-fidelity non-intrusive polynomial chaos (MF-NIPC) has been applied to a structural wind engineering problem in architectural design for the first time. In architectural design it is important to design structures that are safe in a range of wind directions and speeds. For this reason, the computational models used to design buildings and bridges must account for the uncertainties associated with the interaction between the structure and wind. In order to use the numerical simulations for the design, the numerical models must be validated by experi-mental data, and uncertainties contained in the experiments should also be taken into account. Uncertainty Quantifi-cation has been increasingly used for CFD simulations to consider such uncertainties. Typically, CFD simulations are computationally expensive, motivating the increased interest in multi-fidelity methods due to their ability to lev-erage limited data sets of high-fidelity data with evaluations of more computationally inexpensive models. Previous-ly, the multi-fidelity framework has been applied to CFD simulations for the purposes of optimization, rather than for the statistical assessment of candidate design. In this paper MF-NIPC method is applied to flow around a rectan-gular 5:1 cylinder, which has been thoroughly investigated for architectural design. The purpose of UQ is validation of numerical simulation results with experimental data, therefore the radius of curvature of the rectangular cylinder corners and the angle of attack are considered to be random variables, which are known to contain uncertainties when wind tunnel tests are carried out. Computational Fluid Dynamics (CFD) simulations are solved by a solver that employs the Finite Element Method (FEM) for two turbulence modeling approaches of the incompressible Navier-Stokes equations: Unsteady Reynolds Averaged Navier Stokes (URANS) and the Large Eddy simulation (LES). The results of the uncertainty analysis with CFD are compared to experimental data in terms of time-averaged pressure coefficients and bulk parameters. In addition, the accuracy and efficiency of the multi-fidelity framework is demonstrated through a comparison with the results of the high-fidelity model.

RANS simulation of secondary flows in a low pressure turbine cascade: Influence of inlet boundary layer profile

  • Michele, Errante;Andrea, Ferrero;Francesco, Larocca
    • Advances in aircraft and spacecraft science
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    • v.9 no.5
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    • pp.415-431
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    • 2022
  • Secondary flows have a huge impact on losses generation in modern low pressure gas turbines (LPTs). At design point, the interaction of the blade profile with the end-wall boundary layer is responsible for up to 40% of total losses. Therefore, predicting accurately the end-wall flow field in a LPT is extremely important in the industrial design phase. Since the inlet boundary layer profile is one of the factors which most affects the evolution of secondary flows, the first main objective of the present work is to investigate the impact of two different inlet conditions on the end-wall flow field of the T106A, a well known LPT cascade. The first condition, labeled in the paper as C1, is represented by uniform conditions at the inlet plane and the second, C2, by a flow characterized by a defined inlet boundary layer profile. The code used for the simulations is based on the Discontinuous Galerkin (DG) formulation and solves the Reynolds-averaged Navier-Stokes (RANS) equations coupled with the Spalart Allmaras turbulence model. Secondly, this work aims at estimating the influence of viscosity and turbulence on the T106A end-wall flow field. In order to do so, RANS results are compared with those obtained from an inviscid simulation with a prescribed inlet total pressure profile, which mimics a boundary layer. A comparison between C1 and C2 results highlights an influence of secondary flows on the flow field up to a significant distance from the end-wall. In particular, the C2 end-wall flow field appears to be characterized by greater over turning and under turning angles and higher total pressure losses. Furthermore, the C2 simulated flow field shows good agreement with experimental and numerical data available in literature. The C2 and inviscid Euler computed flow fields, although globally comparable, present evident differences. The cascade passage simulated with inviscid flow is mainly dominated by a single large and homogeneous vortex structure, less stretched in the spanwise direction and closer to the end-wall than vortical structures computed by compressible flow simulation. It is reasonable, then, asserting that for the chosen test case a great part of the secondary flows details is strongly dependent on viscous phenomena and turbulence.

Analysis of activated colloidal crud in advanced and modular reactor under pump coastdown with kinetic corrosion

  • Khurram Mehboob;Yahya A. Al-Zahrani
    • Nuclear Engineering and Technology
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    • v.54 no.12
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    • pp.4571-4584
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    • 2022
  • The analysis of rapid flow transients in Reactor Coolant Pumps (RCP) is essential for a reactor safety study. An accurate and precise analysis of the RCP coastdown is necessary for the reactor design. The coastdown of RCP affects the coolant temperature and the colloidal crud in the primary coolant. A realistic and kinetic model has been used to investigate the behavior of activated colloidal crud in the primary coolant and steam generator that solves the pump speed analytically. The analytic solution of the non-dimensional flow rate has been determined by the energy ratio β. The kinetic energy of the coolant fluid and the kinetic energy stored in the rotating parts of a pump are two essential parameters in the form of β. Under normal operation, the pump's speed and moment of inertia are constant. However, in a coastdown situation, kinetic damping in the interval has been implemented. A dynamic model ACCP-SMART has been developed for System Integrated Modular and Advanced Reactor (SMART) to investigate the corrosion due to activated colloidal crud. The Fickian diffusion model has been implemented as the reference corrosion model for the constituent component of the primary loop of the SMART reactor. The activated colloidal crud activity in the primary coolant and steam generator of the SMART reactor has been studied for different equilibrium corrosion rates, linear increase in corrosion rate, and dynamic RCP coastdown situation energy ratio b. The coolant specific activity of SMART reactor equilibrium corrosion (4.0 mg s-1) has been found 9.63×10-3 µCi cm-3, 3.53×10-3 µC cm-3, 2.39×10-2 µC cm-3, 8.10×10-3 µC cm-3, 6.77× 10-3 µC cm-3, 4.95×10-4 µC cm-3, 1.19×10-3 µC cm-3, and 7.87×10-4 µC cm-3 for 24Na, 54Mn, 56Mn, 59Fe, 58Co, 60Co, 99Mo, and 51Cr which are 14.95%, 5.48%, 37.08%, 12.57%, 10.51%, 0.77%, 18.50%, and 0.12% respectively. For linear and exponential coastdown with a constant corrosion rate, the total coolant and steam generator activity approaches a higher saturation value than the normal values. The coolant and steam generator activity changes considerably with kinetic corrosion rate, equilibrium corrosion, growth of corrosion rate (ΔC/Δt), and RCP coastdown situations. The effect of the RCP coastdown on the specific activity of the steam generators is smeared by linearly rising corrosion rates, equilibrium corrosion, and rapid coasting down of the RCP. However, the time taken to reach the saturation activity is also influenced by the slope of corrosion rate, coastdown situation, equilibrium corrosion rate, and energy ratio β.

Effect of Viscosity and Clogging on Grout Penetration Characteristics (점도 변화와 폐색 현상을 고려한 그라우트재의 침투 특성)

  • Kim, Jong-Sun;Choi, Yong-Ki;Park, Jong-Ho;Woo, Sang-Baik;Lee, In-Mo
    • Journal of the Korean Geotechnical Society
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    • v.23 no.4
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    • pp.5-13
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    • 2007
  • Many construction projects adopt grouting technology to prevent the leakage of groundwater or to improve the shear strength of the ground. Recognition as a feasible field procedure dates back to 1925. Since then, developments and field use have increased rapidly. According to improvement of grout materials, theoretical study on grout penetration characteristics is demanded. Fluid of grout always tends to flow from higher hydraulic potential to lower one and the motion of grout is also a function of formation permeability. Viscosity of pout is changed by chemical action while grout moves through pores. Due to the increment of viscosity, permeability is decreased. Permeability is also reduced by grout particle deposits to the soil aggregates. In this paper, characteristics of new cement grout material that has been developed recently are studied: injectable volume of new grout material is tested in two different grain sizes of sands; and the method to calculate injectable volume of grout Is suggested with consideration of change in viscosity and clogging phenomena. The calculated values are compared with injection test results. Viscosity of new grout material is found to increase as an exponential function of time. And lumped parameter $\delta$ of new grout material to be used for assessing deposition characteristics is estimated by comparing deposit theory with injection test results considering different soil types and different injection pressures. Injection test results show that grout penetration rate is decreased by the increase of grout viscosity and clogging phenomena.

Comparison of 1-g and Centrifuge Model Tests for Similitude Laws (상사법칙 검증을 위한 1-g 모형실험과 원심모형실험의 비교 연구)

  • Kim Sung-Ryul;Hwang Jae-Ik;Kim Myoung-Mo;Ko Hon-Yim
    • Journal of the Korean Geotechnical Society
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    • v.22 no.5
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    • pp.59-67
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    • 2006
  • The centrifuge and 1-g shaking table tests were performed simultaneously to compare the dynamic behaviors of loose sands of the same geotechnical properties. The prototype soils were 10 m thick liquefiable loose sands. The geometric scaling factors were 20 for 1-g and 40 for centrifuge tests. The excess pore pressure, surface settlement, and acceleration in the soil were measured at the same locations in the 1-g and centrifuge tests. The total excess pore pressure from development to dissipation was measured. In the centrifuge test, viscous fluid was used as the pore water to eliminate the time scaling difference between dynamic time and dissipation time. In the 1-g tests, the steady state concept was applied to determine the unit weight of the model soil, and two different time scaling factors were applied for the dynamic time and the dissipation time. It is concluded that the 1-g tests can simulate the excess pore pressure of the prototype soil if the permeability of the model soil is small enough to prevent dissipation of excess pore pressure during shaking and the dissipation time scaling factor is properly determined.