Kim, Seul-Ki;Hwang, Hyun-Jin;Kim, Jae-Deog;Ko, Eun-Hye;Choi, Jung-Sup;Kim, Jin-Seog
Korean Journal of Weed Science
/
v.32
no.2
/
pp.85-97
/
2012
To investigate the usefulness of freshwater alga Water-net (Hydrodictyon reticulatum, HR) as resources for production of fermentable sugars, the easiness of enzymatic saccharification was evaluated at first. When 6 plant materials (HR, Spirulina, Chlorella, Scenedesmus, Cladophora, Corn stover) were enzymatically hydrolyzed with 2% solid loading at the same condition, HR showed the highest ratio of saccharification based on glucose production. No milled HR was also completely saccharified at the amounts of optimal enzyme mixture. Glucose yield was not changed though the citrate buffer strength for saccharification was decreased from 0.1 M to 0.1 mM. Only about 10% yield reduction was observed compared to that of $120^{\circ}C$ treatment when HR was enzymatically hydrolyzed at room temperature. The saccharification was normally occurred at $37^{\circ}C$ and pH 6.5 which is general growth condition of fermentable microrganisms, suggesting that HR have a biomass characteristics applicable for the simultaneous saccharification and fermentation. The saccharification was occurred by more than 70~80% of one of the best condition although the supplied enzyme amounts was reduced to 1/10 volume. And the glucose yield by enzymatic hydrolysis was not decreased by 10% HR solid loading and began to decrease at more than 15% solid contents. Above these results show that HR is an interesting algal biomass which is relatively easy to be saccharified by hydrolyzing enzymes. In addition, HR is a flilamentous alga and very easy to be collected. Therefore, HR seems to be an useful and valuable resources in the economical production of fermentable sugars for manufacture of bio-chemical products.
Inactivation in soil absorption, translocation of 2, 4-D by plants vary depending upon soil environments and herbicide formulations. Experiment was conducted in a glasshouse using rectangular pots($1350cm^2$) to evaluate the growth responses of barnyardgrass (Echinochloa crus-galli) and Indian jointvetch (Aesehyrcomene indica) to two formulations of 2, 4-D. The formulations used were 40% 2, 4-D amin salt (2, 4-D/AS) and 19.7% complex of rice husk lignin and 2, 4-D (2, 4-D/LG) which were applied at 200g ai/ha. Soil environments included fertilizer levels, soil pH, organic matter contents, and soil textures, Each treatment was replicated three times. The herbicidal activity of 2.4-D increased and lasted with increased levels of fertilizer. The activity also increased and lasted with low soil pH and decreased content of organic matter. Generally 2, 4-D/LG showed higher and longer herbicidal activity than 2. 4-D/AS for both test plants under all conditions applied. However, the herbicidal activity was influenced by the formulations more than by soil textures. It was thought that 2, 4-D/AS was released in a short time and inactivated readily while 2, 4-D/LG was slowly released and gave an opportunity of absorption by plants for a long period.
The incividual and combined effects of the chloroacetanilide herbicide pretilachlor and of the safener fenclorim on the growth and selected physiological processes of rice (Oryza sativa L., var 'Lemont')were evaluated under greenhouse and laboratory conditions. Fenclorim applied at rates ranging from 50 to 300 g a.i./ha antagonized the injurious effects caused by 150 to 900 g a.i./ha of pretilachlor on 15-day old wet-sown rice grown under greenhouse conditions. When used rates of 150 g/ha or higher, fenclorim reversed completely the effects of all doses of pretilachlor on rice. When the two compounds were given simultaneously, fenclorim enhanced the uptake of $^{14}C$pretilachlor into rice leaf mesophyll protoplasts measured for 1 hr, indicating that competition for uptake at the protoplast level is not involved in the protective action of this safener. The safener-induced stimulation of pretilachlor uptake was particularly evident when fenclorim was used at concentrations of 10, 20 and $40{\mu}M$. Following 4 hr of incubation, individual treatments with pretilachlor inhibited the in vitro incorporation of radiolabeled precursors into proteins, DNA, and lipids of rice leaf protoplasts only when used at the high concentration of $100{\mu}M$M. Individual treatments with high concentrations (10 or $100{\mu}M$) of the safener fenclorim inhibited the incorporation of radiolabeled precursors into proteins and lipids of rice protoplasts, but had no DNA synthesis. The combined effects of pretilachlor and fenclorim on the incorporation of radiolabeled precursors into these macromolecules of isolated rice mesophyll protoplasts appeared to be additive or slightly synergistic rather than antagonistic. Fenclorim at $1{\mu}M$ antagonized the effects of pretilachlor on total lipids of rice leaf protoplasts. In addition, individual and combined treat-menu with pretilachlor and fenclorim influenced the incoroporation of$^{14}C$acetate into polar lipids, triglycerides and steryl esters of rice leaf protoplas causing a redistribution of carbon in these lipid fractions. However, these effects were not large enough to explain the herbicidal activity of pretilachlor or to account for the protective action of the safener fenclorim. Overall, the uesults of the present study idnicate that the safener fenclorim does not seem to protect rice against pretilachlor injury by antagonizing its effects on protein, DNA, or lipid syntheses.
Weed control is of fundamental importance when planting horticultural crops, particularly during the establishment phase. Weeds compete for nutrients, water and light, and can severely threaten the survival and early growth of newly planted crops. Failure to control weeds represents one of the single most important factors leading to crop loss. Knowledge on the existence of the diversity of weed species in greenhouses is of our main concern in this study in order to develop a most efficient and effective weed control strategies. Sixty-two greenhouses were surveyed in 3 cities and counties of Gyeongnam area in March to October 2009 to investigate the feature of weed occurrence in polyvinyl chloride (PVC) after harvesting of the main crops. Forty-one weed species were identified and classified to 18 families which were composed of 14 annual weeds, 18 summer annual weeds and 9 perennial weeds. On the other hand, broadleaf, grasses and sedges recorded with 30, 7 and 4 weed species, respectively. Asteraceae was the most dominant weed species (9 species) noted followed by Poaceae (7 species), Cyperaceae (4 species), Caryophyllaceae and Brassicaceae (3 species respectively) and other families have 1~2 species. The dominant weed species occurred in the greenhouse based on the summed dominance ratio. These weeds were Digitaria sanguinalis, Cyperus iria, Portulaca oleracea, Rorippa islandica, Mazus japonicas, Cardamine flexousa, and Eclipta prostrata and others. Weed occurrence in the greenhouse after horticultural crops consisted of summer annuals (4 species), winter annuals (3 species), and perennial annuals (1 specie). The dominant species occurred in tilled soil based on summed dominance ratio of weeds were Cardamine flexousa (88.1%), Eclipta prostrate (57.4%) and Portulaca oleracea (55.2%). Comparison of weed occurrence was thoroughly surveyed also in which field without PVC, weed species were Portulaca oleracea (55.2), Eclipta prostrata (57.9%) and Trigonotis peduncularis (25.1%) and field with PVC, the identified weeds were Portulaca oleracea (98.75), Trigonotis peduncularis (49.1%), and Eclipta prostrata (36.8%).
The objective of this research was to find out the weed management techniques in environment-friendly rice paddy fields through the study on herbicidal effects and problems of various organic materials. This experiment was conducted under different conditions of weed species and weed densities in environment-friendly rice paddy fields. There was no difference in weedy efficacy on golden apple snail (GAS), paper mulching (PM), and machine weeding (MW) between low and high weed densities. However, the effect of weed control in rice bran (RB) and effective microorganism (EM) + molasses was higher in high weed density than in low weed density. In general, the effect of weed control as affected by various organic materials was in the order of GAS (97-100%) > PM (93-98%) > RB (15-80%) > EM (7-31%). GAS provided excellent control of all weed species tested except for Persicaria hydropiper. PM gave acceptable control of the weed species except for Echinochloa crus-galli, Ludwigia prostrata, and Eleocharis kuroguwai. However, MW gave fair control (70% biomass reduction) of all weed species tested. BR followed by MW or EM followed by MW treatments had similar effect on weed control compared to each treatment alone. However, BR followed by GAS or EM followed by GAS provided 100% control of weed species tested. The level of rice foliar injury caused by various organic materials was in the order of GAS and MW (10-20%) > RB (10-15%) > PM and EM (5-7%). Typical symptoms of organic materials are wilting, inhibition of growth, missing hill, and reduction of tiller. Cost for weed control of GAS, RB, EM, and PM were 2.1, 3.1, 2.3, and 13.2 times higher than that of the herbicide. These data indicate that GAS was the best method for weed management in environmentfriendly rice paddy fields. Further study is required to elucidate the mechanisms underlying the rice injury as affected by GAS.
To study ecological characteristics of Sagitiaria pygmaea and Sagittaria trifolia occurring in Korea their propagules were collected from 3 locations (Sagittaria pygmaea: Chuncheon, Suweon, Milyang; Sagittaria trifolia: Suweon, Iri, Jeonju) in 1981, cultured and replanted 4 times (May 20, June 5, June 20, July 5) in 1982. Sagitraria pygmaea from Suweon flowered earlier than those from Chuncheon and Milyang in the plants planted on May 20, but this was reversed in another planting dates. Three storied inflorescence was observed newly in Sagittaria pygmaea. Sagittaria pygmaea from Iri and Jeonju had more number of tillers, but less number of tubers per tiller than those from Suweon. Sagittaria rrifolia from Chuncheon flowered earlier than those from Suweon and Milyang. Sagirtaria trifolia from Milyang was narrower in the upper leaf width and less in the number of tubers per plant than those from Chuncheon and Suweon. Each of local collections may be regarded as different ecotype based on the above differences.
KSC-13906 [Erythro N-{(4,6-dimethoxypyrimidin-2-yl)aminocarbonyl} -2-(2-fluoro-1-hydroxy-n-propyl) benzenesulfonamide, US Patent 5,461,025] was investigated how can control phytotoxicity fluctuation and what a good method apply to new rice herbicide. The growth inhibition was observed when the rice plants was transplanted at a shallow depth(0 - 1cm) and leaching was low(0 - 1cm/ day) from the paddy soil. KSC-13906 appeared to move readily down into the paddy soil with water by 3cm depth in the soil column(${\phi}$ 10cm) filled with loamy sand soil under 3cm/day of leaching condition. Artificial control releasing pattern, designed as treated with KSC-13906 of 9 or 18g ai/ha either at a once or daily treated dividing volume of 1/20, 1/25 and 1/30 of the total volume, increased the safety of KSC-13906 to direct seeded and transplanted rice. The safety of KSC-13906 was also enhanced when KSC-13906 was mixed with dymron. For example, the mixture of KSC-13906 and dymron effectively reduced injury of direct seeded rice plants at 18 and 500g ai/ha, respectively, treated 7 days after transplanting. However, combination of KSC-13906 and several herbicides didn't show any synergistic effetct on herbicidal activity and safening effect on rice. However, the combination of KSC-13906+dymron (9~12+250~500g ai/ha) or KSC-13906+mefenacet+dymron(9+250+250g ai/ha) controlled almost all weeds in paddy field without causing any injury to rice and thus the combination would successfully be used as an oneshot herbicide in rice culture.
This experiment was conducted to evaluate the effect of plant growth regulators, dichlorprop and MCPB on the reduced effect of fruit drop and fruit quality before and after storage in apples. Dichlorprop was tested with dilution of 1000 at 30, 40, 50 days before harvesting, and MCPB with dilution of 4000 at 15, 25, 35 days before harvesting. The results are summarized as follows : Percentage of fruit drop was appeared to the notable reduction as compared with the untreated control when regulators was applied with dilution of 1000 at 30 days before harvesting by dichlorprop and with dilution of 4000 at 35 days before harvesting by MCPB. Degree of fruit colour showed to the remarkable promotion at all the treatment of 30, 40, 50 days before harvesting by dichlorprop as compared with the untreated control. Sugar contents in flesh was increased a little at the treatment of 30 days before harvesting by dichlorprop, but acid contents in flesh was reduced at all the treatment of 30, 40, 50 days before harvesting by dichloroprop and at 15, 25, 35 days before harvesting by MCPB. Passed firmness of fruit after storage was maintained at the treatment with dilution of 4000 at 35 days before harvesting. Therefore, it was repressed a softening of fruit, but by dichlorprop treatment at 30, 40, 50 days before harvesting, fruit firmmess was appeared to reduce according to the passage of storage period. Amount of ethylene evolution after storage was showed to reduce at all the treatment by early treated time of dichoroprop and MCPB, but carbon dioxide increased at treatment conditions such as the front. Accordingly, these relationship showed to be contrary each other.
To know the ecological pattern of bulrush (Scirpus juncoides Roxb.) seeds and overwintreed stumps in germination and sprouting responses as affected by different temperature (7 trt.), light intensity (5 trt.), shading intensity (S trt.), light quality (specturm spectrum; 6 trt.), soil acidity (7 trt.), stump sizea (weight base; 5 trt.), and molding depth (6trt.), respectively, this serial studies were conducted by use of growth chamber, incubator, Wagner pot and petri-dish. Most efficient treatment was obtained from 25-$35^{\circ}C$ temperature, higher light intensity in 2-11 klux range, 95% shading intensity, clear and yellow film for seeds/clear and blue film for stumps, soil pH 5.53, 3-4g stump weight, 0-5% wxygen concentration, 1 ㎝ flooding depth for seeds, and 1-1.5cm molding depth for seeds/0.5-1.0 cm molding depth for stumps, respectively, among others.
At 5 DAS/T, leaf primordia of rice stems that were grown under dry condition in transverse sections were strongly stained while those under water condition had many aerenchyma cells well developed. On the other hand, leaf primordia and large air spaces in stem of transplanted rice were well developed. Rice in leaf anatomy had small and fine epidermal cells, chlorophyllous mesophylls, and bulliform cells but had no chlorophyllous vascular bundle sheath cells, while barnyardgrass leaf had large, rough and irregularly arranged epidermal cells, chlorophyllous vascular bundle sheath cells, and non-bulliform cells but had no chlorophyllous mesophylls. Epidermal cells of transplanted rice, however, were well developed, differentiated and sclerified. Cross sections of rice root under dry condition showed cell contents, regularly arranged cells, non-intercellular spaces and non-aerenchyma while under water condition had well-developed intercellular spaces, aerenchyma cells, small and densely arranged epidermis, sclerified exodermis and sclerenchyma cells. But root anatomy of transplanted rice consisted of finely, regularly arranged epidermis, well-developed intercellular spaces and nucleous cells.
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