• 미생물학회지
• /
• 제32권1호
• /
• pp.60-64
• /
• 1994

자연계로부터 식물병원균인 Pseudomonas syringae pv. Tabaci에 감염하는 bacteriophage 를 분리하였다. 이 phage의 안정성을 조사한 결과 중성 부근 pH에서 가장 안정하였고 50${\circ}C$ 이상에서는 안정성이 급격히 감소하였으며 흡착시간 별로는 10분까지는 빠른 흡착율을 보이다가 그 후부터는 서서히 감소하였다. 또한 흡착에는 금속이온을 필요로 하였으며 흡착온도는 20${\circ}C$에서 가장 높게 나타났고 20~40분에서 가장 높게 나타났다. 또한 배양온도에 따라 plaque 양상이 달랐는데 10${\circ}C$에서는 clear plaque를, 20, 30${\circ}C$에서는 turbid plaque를 형성하였다. 20${\circ}C$에서 잠복기는 약 3시간이었고 평균 방출수는 200PFU/cell이었다. 유전물질로는 ds DNA를 가지고 있었고 크기는 30kb 정도이었다.

• 미생물학회지
• /
• 제17권4호
• /
• pp.165-178
• /
• 1979

Phages of Lactobaciilus casei (PLC) isolated from plant drainage were classified and characterized. The results are as follows : 1. On the basis of host range pattern, phages could be divided into 2 groups (PLC-B and PLC-C). PLC-B group phages could be further divided into 5 sub-groups $(B_1, \;B_2, \;B_3, \;B_4, \;and\;B_5)$. Although PLC-C group phages had the same host range, they could be also divided into 2 sub-groups $(C_1\;and\;C_2)$ by morphlogical type. 2. It was $B_3$ group phages that represented a major proportion (44.4%) of phages tested. However, $B_1$ group phages were shown to have the widest host range. 3. Electron micrographs revealed that the phages fell into three different morphological types. $(B_1, \;B_2, \;and\;B_3)$ group phages hd a hexagonal head (52nm in diameter) and a sheathless noncontractile (245 nm in length). $B_4\;and\;C_2$ group phages had a hexagonal head (56 nm) and a short flexible tail (169nm) having no sheath. $B_5\;and\;C_1$ group phages were shown to have a hexagonal head (81 nm) and a contractile tail (140 nm) having a sheath, a base plate and tail fibers. 4. The inactivation of the phages by antisera indicated that serological relationships correlated completely with morphological types. 5. $B_1, \;C_1\;and\;C_2$ group phages produced a large (1, 2 mm in diameter) plaque with a clear ring. The morphology of plaques of $B_3\;and\;B_5$ group phages was the same as those produced by the above, but the average plaque sizes for $B_3\;and\;B_5$ were 0.8 mm abd 0.5 mm, respectively. $B_2\;and\;B_4$ group phages produced a small (0.5 mm) turbid plaque with an irregular edge. 6. The latent period and the average burst size of $B_1\;and\;B_3$ group phages were 90 min and 100, respectively. These phages reuqired calcium ions for their miltiplication. 7. $B_3$ group phages could not be absrobed to R-variant $KC_1$. 8. The order of resistance of phages to heat was $B_2\;>\;B_1, B_4\;and\;B_5\;>\;B_3\;and\;C_2, \;B_5$ group phages were more stable than $B_3$ in various pH values. $C_2$ group phages were more sensitive to UV irradiation than $B_1\;and\;B_3$ group phages. 9. Strains YIT9018 and IAM 1043 were induced by mitomycin C treatment. Phage particles detected in the lysates had a hexagonal head (38 and 49 nm, respectively), but no tail. Any sensitive indicator strain could not be isolated in spite of repaeated trials.