• 한국식물병리학회지
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• 제3권4호
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• pp.239-244
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• 1987

감자 걀쪽바이로이드(PSTV) RNA분자를 포함한 저분자량의 식물성 RNA분자에 대해서 0M부터 8M까지의 요소농도구배를 가진 폴리아크릴아마이드겔을 이용하여 1/40, 1/10로 희석한 완충액조건과 $17^{\circ}C,\;37^{\circ}C,\;57^{\circ}C$의 온도조건에서 전기영동을 실시하였다. 바이로이드의 RNA분자가 환상과 선상의 두가지 분자로 나뉘어지는 변성조건에서는 1/40로 희석한 TBE완충액을 포함하는 요소농도구배겔을 $57^{\circ}$에서 전기영동을 실시하는 것이 효과적이었다. 변성된 환상의 바이로이드분자에 대한 전기 영동적 이동성은 주로 요소의 농도에 따라 영향을 받는 것으로 보이는데, 이와 더불어 저 농도의 TBE완충액이 요소농도구배겔에서 환상과 선상바이로이드분자 사이에서 전기영동적 분리를 증가시켜 주었다.

• Toxicological Research
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• 제6권1호
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• pp.41-49
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• 1990

Aconiti Tuber is the root of Aconitum sp (Ranunclaceae) which has been considered as one of the most important medicinal plant having cordiotonic, diuretic and analgesic effect. On the other hand, it has been known that Aconiti Tuber contained toxic agent, aconitine alkaloids so that only processed Aconiti Tubers have been used as herbal drug traditionally. For the safety evaluation of processed Aconiti Tuber, quantitative determination of aconitine and acute, subacute toxicity test were performed on 5 commercial processed Aconiti Tubers. Arapid and precise method using HPLC has been developed for the separation and determination of aconitine. Samples were extracted with hydrochloric acid (pH3) and hot water decoction. In case of d-HCL extracts, the contents of aconitine were from 0.08 mg/g to trace. But in case of hot water decoction extracts, the contents of aconitine were not detected. For the investigation of Aconiti Tuber toxicity in rats, hot water decoction samples and methanol extracts were tested. 1) Acute toxicity test Hot water decoction sample and methanol extracts from Aconiti Tuber did not show any toxic effects in rats by an oral administration. $LD_50values of 2 extracts were above 10.0 g/kg. 2) Subacute toxicity study In the repeated administration study, hot water decoction samples were given orally to Sprague-Dawlay rats for 2 week at daily doses of 5.0 g/kg. The results are as follows; No toxic manifestation, body weight changes and lethality were observed during wxperimental period. There were no significant changes in serum enzyme activities such as GOT, GPT, LDH, ALP between treated and control groups. However CPK values were decreased in the Subuja-treated group. (P<0.01). In addition, no gross and microscopic changes were noted in Aconiti Tuber-treated groups.

• 한국작물학회지
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• 제46권4호
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• pp.309-316
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• 2001

Helianthus tuberosus has been known to inhibit the growth of weeds and other plants sharing its habitat. This study was conducted to identify the allelochemicals of Helianthus tuberosus which were extracted with water and solvents. Aqueous extracts of leaf, stem, root, tuber and tuber peel of Helianthus tuberosus except tuber did not show significant differences in phytotoxicity to alfalfa seedlings. It was considered that Helianthus tuberosus contained fewer or less potential water-soluble substances that were toxic to alfalfa. Methanol extract of leaves of Helianthus tuberosus was sequentially partitioned in increasing polarity with n-hexane, ethylacetate and n-butanol. Each extract had a yield of 148, 12, 15.7 and 9.5g, respectively. Inhibitory effects on germination of alfalfa seeds treated with four fractions were not significantly different. But the significant reductions on hypocotyl length were observed for all the solvent extracts. Among the four fractions, the ethylacetate fraction showed the most significant inhibition effect on bioassay with alfalfa. Further separation of the active ethylacetate fraction by open column chromatography led to the 25 subfractions. In bioassay of each sub-fraction with alfalfa seeds, sub-fraction No. 13 showed the most inhibitory effect on seedling growth. $^1$H NMR and gas chromatography-mass spectrometry analysis revealed that sub-fraction No. 13 was the mixture of straight-chain saturated fatty acids.

• Plant Resources
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• 제6권1호
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• pp.81-88
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• 2003

Higenamine [1-(4-hydroxy-6, 7-dihydroxy-l, 2, 3, 4-tetrahydroisoquinoline) is a cardiotonic constituent of Aconiti tuber, one of the most widely prescribed oriental medicines. S-(-)higenamine was reported to have a stronger cardiotonic activity than R-(+)-higenamine and known as a central intermediate in the biosynthesis of various benzyl isoquionoline alkaloids in plants. The separation of higenamine enantiomers has been accomplished with capillary electrophoresis using cyclodextrins (CDs) as chiral selectors. Good resolution of this enantiomers was obtained using a 50 mM sodium phosphate buffer containing hydroxypropyl $\beta$-CDs using 27 cm fused silica capillary (50${\mu}{\textrm}{m}$ i.d., 20 cm to detector) at 25 $^{\circ}C$. With the electric field of 340 V/cm, the separation time of higenamine enantiomers was less than 6 min. Under this optimum conditions, the relative standard deviations of migration time and peak area were less than 1.6% and 3.2%. A 512-channel diode array detector was confirmed for the higenamine. The detection limits (S/N = 3) of these enantiomers are $1.5mutextrm{m}$/mL. We confirmed the chiral form of higenamine in medicinal plants.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1993년도 Proceedings of International Conference for Agricultural Machinery and Process Engineering
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• pp.734-743
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• 1993

The performances of three same type of vibrating potato diggers were estimated by observing the potato separation and material flow on the bottom plate. Four-bar mechanism were adopted for three diggers and pairs of eccentric cams on both sides of driving shaft were used as driving link of the diggers. Each machine was tested with different amplitudes , frequencies, and travels speeds. Blade performance were observed in three categories : Impossible forward travel , acceptable operation, and unsatisfactory potato digging , but good material flow. Three parameters were used to set marginal values that enable the machines operate for potato digging, and the parameters were compared to select best one. Three parameters are λ, $\rho$, and K.λ is the ratio of vibrating speed to travel speed, $\rho$ is the ratio of blade acceleration to travel speed, and K is the ratio of blade acceleration to gravitational acceleration. K value of 2 or more is suggested to be used as design and evalu tion criterion of the vibrating digger.

• 한국식품과학회지
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• 제5권1호
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• pp.65-69
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• 1973

여러가지 선량(線量)의 방사선조사(放射線照射)가 감자 박편(薄片)$(1\;cm{\times}2\;mm)$ 의 치유조직(治癒組織) 형성에 미치는 효과를 무균 상태하에서 조사(調査)하였다. 세포분열은 선량(線量)이 증가함에 따라 점차 억제되었고 16 krad 처리에서는 완전히 억제되었다. Solanine 함량은 선량(線量)에 따라 유의적(有意的)인 차이를 나타내지 않았다. 방사선조사(放射線照射)에 의하여 감자 괴경(塊莖) 내에 유기(誘起)된 carbonyl 화합물로부터 생성된 2,4-dinitrophenylhydrazone 침전물을 gas chromatograph 로 분리한 결과 formaldehyde 및 acetone 은 고선량(高線量)에서만 증가하는 경향을 보였다.

• 한국작물학회지
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• 제31권1호
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• pp.30-42
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• 1986

반하〔Pinellia ternata(Thunb.) Briet〕의 조직을 기내배양하여 callus 유기, 기관분화 및 자구를 증식시키는데 적합한 배지와 배양부위를 선정코저 Mur-ushige & Skoog 배지에 2,4-D와 kinetin의 첨가량을 달리하여 검토하였고 또한 배양온도 및 배지의 pH 영향을 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. Callus 유기는 배양 5~7일 후에 엽조직 및생장점조직의 절단면에서 관찰할 수 있었으며 2,4-D 2.0mg/$\ell$＋kinetin 0.2mg/$\ell$을 첨가한 구에서 callus의 형성 및 생장이 가장 양호하였다. 2. 자구의 형성은 엽조직 및 생장점조직을 2,4 -D 2.0mg/$\ell$＋kinetin 0.2mg/$\ell$ 또는 2.4-D 2.0g/$\ell$＋kinetin 2.0mg/$\ell$을 첨가한 배지에서 양호하였고 근 및 괴경조직에서는 자구가 전혀 ？성되지 않았다. 3. 엽의 부위별 자구형성능력은 Petiole이 자구형성수, 크기, 엽면적 및 생체중에 있어서 marginal meristem, minor vein area 및 intercostal area 보다 양호하였다. 4. 경의 부위별 자구형성능력은 상부위 조직일수록 자구의 형성수 크기, 엽면적 및 생체중이 양호하였으나 하부위일수록 불량하였다. 5. 배양온도는 $25^{\circ}C$에서 callus 유기, 크기 및 생체중이 가장 양호하였다. 6. 배지의 pH는 4.5~7.5에서 모두 callus가 유기되었으나 그중 pH 6.0 배지에서 가장 양호하였다. 7. 재생되는 식물체 일부가 광엽화되는 경향이었으며 소수의 albino 식물체와 변이체도 관찰할 수 있었다.