• Journal of Acupuncture Research
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• v.24 no.5
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• pp.23-32
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• 2007

Objectives : Angiogenesis consists of the proliferation, migration, and differentiation of endothelial cells, and angiogenic factors and matrix protein interactions modulate this process. The aim of this study was to determine whether herbs medicine(KHBJs) could induce angiogenic activity in human umbilical vein endothelial cells(HUVECs). Methods : The angiogenic activity of KHBJs were evaluated by proliferation using BrdU assay, chemotactic migration assay, tube formation assay, and measurement of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor(VEGF) in HUVECs. Also, In order to identify enhance angiogenic activity by activity guided fractionation, the angiogenic activity of fractions of KHBJs such as KHBJB or KHBJR were evaluated in vitro and in vivo Matrigel plug angiogenesis asaay. Results : About 9 KHBJs significantly increased HUVECs proliferation in a dose-dependent manner. In addition, 9 herbs medicine(KHBJs) increased migration and tube-like formation in HUVECs. Interestingly the expression of bFGF and VEGF, an angiogenesis-inducing growth factor, were dose-dependently increased by KHBJs. However, angiogenic activity of fractionated KHBJs(KHBJB or KHBJR) not enhanced more than KHBJs in HUVECs and Matrigel plug in vivo angiogenesis assay. Conclusions : 9 KHBJs significantly induces angiogenesis in in vitro and in vivo. These results suggest that 9 KHBJs potent angiogenic agents and promising drug for the induction of neovascularization.

• The Plant Pathology Journal
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• v.39 no.1
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• pp.123-135
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• 2023

Previously, Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 from a sequential screening procedure were proven to effectively control Phytophthora blight caused by Phytophthora capsici. In this study, we further investigated the anti-oomycete activities of these strains against mycelial growth, zoospore germination, and germ tube elongation of P. capsici. We also investigated root colonization ability of the bacterial strains in square dishes, including cell motility (swimming and swarming motilities) and biofilm formation. Both strains significantly inhibited mycelial growth in liquid and solid V8 juice media and M9 minimal media, zoospore germination, and germ tube elongation compared with Bacillus vallismortis EXTN-1 (positive biocontrol strain), Sphingomonas aquatilis KU408 (negative biocontrol strain), and MgSO₄ solution (untreated control). In diluted (nutrient-deficient) V₈ juice broth, the tested strain populations were maintained at >10⁸ cells/ml, simultaneously providing mycelial inhibitory activity. Additionally, these strains colonized pepper roots at a 10⁶ cells/ml concentration for 7 days. The root colonization of the strains was supported by strong swimming and swarming activities, biofilm formation, and chemotactic activity towards exudate components (amino acids, organic acids, and sugars) of pepper roots. Collectively, these results suggest that strains YJR13 and YJR92 can effectively suppress Phytophthora blight of pepper through direct anti-oomycete activities against mycelial growth, zoospore germination and germ tube elongation. Bacterial colonization of pepper roots may be mediated by cell motility and biofilm formation together with chemotaxis to root exudates.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.4
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• pp.323-328
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• 2017

Angiogenesis is a process including members of the angiogenic factors. In particular, fibroblast growth factor 2 (FGF2) is considered the most potent angiogenic factor because it promotes cell proliferation and tube formation. A recent study reported that fucoidan derived from marine plant potentiated FGF-2 induced tube formation in human endothelial cells. On the other hand, the molecular mechanisms involved in the angiogenic activity of fucoidan and FGF2 are unknown. In this study, a fucoidan treatment promoted angiogenesis induced by FGF2. The effects of fucoidan on FGF2-induced angiogenesis were confirmed by a proliferation assay using a CellTiter96 Aqueous One solution after a treatment with fucoidan and FGF2. The tube formation and wound healing assay for the angiogenic activity were also confirmed. Reverse transcription PCR showed a change in the mRNA of vascular endothelial growth factor-A (VEGF-A), intercellular adhesion molecule-1 (ICAM-1), matrix metallopeptidase9 (MMP9), and the signal transducer and activator of transcription3 (STAT3). In summary, the Fucoidan/FGF2 treatment induced an increase in cell proliferation, improved the tube formation and wound healing activity, and altered the STAT3, VEGF-A, ICAM-1, and MMP9 mRNA expression levels. Further research will be needed to provide a scientific explanation in terms of cell-signaling and confirm the present findings.

• Journal of the Korean institute of surface engineering
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• v.55 no.1
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• pp.1-8
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• 2022

Titanium dioxide (TiO₂) is one of the most intensively investigated materials in materials science. Mostly, TiO₂ has been used in the form of nanoparticles, but recently new highly ordered TiO₂ nanotubes (U-tube) has been introduced and applied to various applications due to their one-dimensional charge path way. In the present paper, we described the formation process and physical properties of U-tube then, gave examples of applications in sequence. Firstly, in photocatalysis, U-tube was used with Au/Pt co-catalysts and showed enhanced photogenerated H₂ efficiency compared to bare TiO₂. Secondly, photoelectrochemical performance of U-tube was evaluated with different heat-treatment temperatures. As a further application, two different types of electrical cell (Ti-TiO₂-Pt and Ti-TiO₂-Pt_NP) was configurated to observe memristive behavior of U-tube. Both cells behaved as switching electrodes and follow a memristive movement in the high and low resistance state extremely well with high reproducibility.

• Steel and Composite Structures
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• v.50 no.2
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• pp.217-235
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• 2024

Concrete-filled steel tubes (CFSTs) nowadays are widely used as the main parts of momentous structures, and its connection has gained increasing attention as the complexity in configuration and load transfer mechanism. This paper proposes a novel CFST pier-to-footing incorporating tube-confined RC encasement. Such an innovative approach offers several benefits, including expedited on-site assembly, effective confinement, and collision resistance and corrosion resistance. The seismic behavior of such CFST pier-to-footing connection was studied by testing eight specimens under quasi-static cyclic lateral load. In the experimental research, the influences on the seismic behavior and the order of plastic hinge formation were discussed in detail by changing the footing height, axial compression ratio, number and length of anchored bars, and type of confining tube. All the specimens showed sufficient ductility and energy dissipation, without significant strength degradation. There is no obvious failure in the confined footing, while local buckling can be found in the critical section of the pier. It suggests that the footing provides satisfactory strength protection for the connection.

• Journal of Ginseng Research
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• v.47 no.2
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• pp.246-254
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• 2023

Background: Here, we aimed to assess the inhibitory effect of a new compound from Panax ginseng on the migration of human ovarian cancer cells and tube formation of human umbilical vein endothelial cells (HUVECs). Methods: A new compound, ginsenglactone A (1), was isolated from ginseng roots, together with seven known compounds (2-8). Spectroscopic data were used to elucidate the chemical structure of 1. The tubular structure formation in HUVECs was assessed by Mayer's hematoxylin staining. The migration of A2780 cells was evaluated using the scratch wound healing assay. Results: HUVECs treated with 1 had the statistically significant decrease in tubular structure formation compared to the HUVECs treated with compounds 2-8. This effect was enhanced by co-treatment with inhibitors for phosphatidylinositol 3-kinase (PI3K) (LY294002) and extracellular signal-regulated kinase (ERK) (U0126). Treatment with 1 decreased the expression of phosphorylation of ERK, PI3K, vascular endothelial growth factor receptor2 (VEGFR2), Akt, and mammalian target of rapamycin (mTOR). In addition, the ability of A2780 cells to cover the scratched area were also decreased. This effect was enhanced by co-treatment with U0126. Lastly, treatment with 1 decreased the phosphorylation of ERK, matrix metalloproteinase-9 (MMP-9), and MMP-2. Conclusion: These results suggest that ginsenglactone A is a potential inhibitor of HUVEC tubular structure formation and A2780 cellular migration, which may be helpful for understanding its anticancer mechanism.

• Resources Recycling
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• v.24 no.2
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• pp.3-12
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• 2015

We researched characteristics of heat recovery rate and fouling according to structures and materials in heat exchangers like water preheater and air preheater. Economizer and air preheater have used in thermal electric power plant. we made small incinerator and heat exchangers to carry out simulated experiment. We observed fouling formation and change of heat recovery rate, combusting powdered coal for 24 hr. In economizer, fin tube type had the largest amount of fouling formation, followed by tube line type > pipe type > auto washing type according to structures. As heat recovery rate, fin tube showed highest recovery rate, followed by auto washing type > pipe type > tube line type. In air preheater, fin tube type had the largest amount of fouling formation, followed by fin plate type > pipe type > pipe type coated by teflon > pipe type coated by ceramic according to structures. And then, heat recovery rate showed the same oder.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.413-417
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• 1998

Magnaporthe grisea, the casual agent of rice blast, requires formation of an appressorium, a dome-shaped and well melanized infection structure, to penetrate its host. Environmental cues that induce appressorium formation include hydrophobicity and hardness of contact surface and chemicals from its host. Artificial surfaces are widely used to induce appressorium formation, but frequencies of appressorium induction are not always consistent. To understand variable induction of appressorium formation in M. grisea, several factors were tested on GelBond. High levels of appressorium formation were induced over a wide range of temperature (20~3$0^{\circ}C$) and pH (4~7). spore age up to 3-week-old did not significantly affect appressorium formation, but only a few apressoria on GelBond. However, adenosine specifically inhibited appressorium formation. Adenosine inhibition of appressorium formation was restored by exogenous addition of cAMP. Germ tube tips of M. grisea maintained the ability to differentiate appressoria by chemical inducers on GelBond at least up to 16 h after conidia germination. These results suggest that environmental factors have little effect on the variable induction of appressorium formation on the artificial surface in M. grisea.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.2
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• pp.47.2-47.2
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• 2021

The present study is focused on origins of the flow and magnetic structure involved in the formation and eruption of a solar prominence. To clarify them, we performed an MHD simulation based on the 3-dimensional emerging flux tube (3DEFT) model, in which self-consistent evolution of a flow and magnetic field passing freely through the solar surface was obtained by seamlessly connecting subsurface dynamics with surface dynamics. By analyzing Lagrangian displacements of magnetized plasma elements, we demonstrate the flow structure which is naturally incorporated to the magnetic structure of the prominence formed via dynamic interaction between the flow and magnetic field.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.1
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• pp.85-96
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• 2016

A series of tests were conducted to examine the filled tube shape with respect to the filling module type used and to investigate cone resistance properties of a dredged-soil-filled geotextile tube under water and drained conditions. Results based on the filling observation showed that the distribution of the accumulated fills inside the acrylic cell and vinyl tubes differs with respect to the type of filling modules. A crater formation around the inlet area was found during the test using I-type filling module and a horizontal sediment distribution was found during the test using inverse T-Type filling module. The dredged fill material was obtained from the Saemangeum area. The geotextile tube deformation of each filling stage was almost converged when the tube was fully drained. The cone resistance of the dredged fill in the geotextile tube under drained condition is large and is approximately 2~6 times that of the tube under water condition.