• Journal of the korean academy of Pediatric Dentistry
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• v.43 no.3
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• pp.299-305
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• 2016

The aim of this study was to identify the causative genetic mutation in a family with non-syndromic oligodontia. The 7-year-old female proband and her mother underwent oral examination, panoramic radiographs were obtained and blood samples were collected. All exons of the PAX9 gene were amplified by polymerase chain reaction and sequenced. The sequencing results were compared with the standard human gene sequence. The proband lacked 11 permanent teeth, and her mother lacked 19 permanent teeth. No other birth defects were observed. As a result of gene analysis, there was a novel heterozygous nonsense mutation (c.184G>T, $p.Glu62^*$) in exon 2 in both affected subjects. It is suspected that the nonsense mutation leads premature termination of translation, yields a truncated protein 280 amino acids shorter than the wild-type protein. These defects include parts of the paired box domain, a DNA-binding site that plays an essential role in protein function. Otherwise, more likely the mutant transcript would be degraded by nonsense-mediated decay system, resulting haploinsufficiency to cause oligodontia in this family.

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.4
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• pp.587-596
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• 2006

Most of oral streptococci express the Antigen I/II (AgI/II) proteins, cell wall anchored adhesions. AgI/II protein binds to salivary agglutinin glycoprotein, a component of tooth pellicle and to ligands in other bacteria. These associations play important roles in bacterial colonization. Recently, it was reported that diverse host molecules also interact with AgI/II protein and that these interactions induce inflammatory responses from host cells. Among mutans streptococci containing -type hemolytic activity, Streptococcus mutans is a causative agent for dental caries. Compared with many other strains of S. mutans, GS-5 strain is unique in that this bacterium expresses truncated secretory AgI/II protein due to the nonsense mutation in the agI/II gene. This indicates that S. mutans GS-5 has a different clinical role and a recent report supported this idea based on the results from clinically isolated S. mutans strains. Previously, we had cloned agI/II gene from S. mutans GS-5 and generated recombinant N-terminal AgI/II protein. In this study, we further produced a hybridoma line expressing anti-AgI/II monoclonal antibodies named as 1C11A. This antibody showed high sensitivity to AgI/II protein in Western blot and ELISA. This new reagent will provide a basis for investigating the mechanisms of AgI/II-related diseases.

• 한국해양학회지
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• v.29 no.3
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• pp.217-227
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• 1994

From northeast to southwest, discontinuous sand ridges distribute on the western continental shelf of Korean Peninsular. The dimension of sand ridges is 3 to 21 m high, 3.1 to 6.8 km wavelength and 9-64 km long with 0.5 steep slope. they are probably originated and reformed by the intensity of tidal current according to the sea level rise. The characteristics of sand ridges revealed in study area are summarized as follows: (1) The sand ridges line up with the long axes of the tidal current ellipses, indicating a tidal control. (2) these are composed of two sedimentary sequences on the 3.5 kHz seismic profiles and core sediments. The upper sequence characterized by prolonged type is covered with thin veneer of massive fine sand(Mz, 2-3$\phi$) with Olive Gray(5Y 5/2). The lower sequence is characterized by internal reflector type with parallel and discontinuous. It consists of sandy mud or muddy sand(Mz, 5-7$\phi$) with laminar structures. the parallel internal reflectors are truncated on the slope of sand ridges. (3) Asymmetrical sand waves are superimposed on the sand ridges, and facing to the crest. However, symmetrical sand waves lie on the crest. Sand ridges having characteristics above is originated by scouring of tidal current, covered with coarase relict sediments, and modified by sadware.

• Korean journal of food and cookery science
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• v.27 no.1
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• pp.63-73
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• 2011

Cabbage (Baechu) Kimchi in its truncated form was placed in four different packing materials, Ny/PE/LLDP, OPP/AL/PE, PP and PET, and quality changes were observed during storage. Changes in pH and total acidity showed an x-shaped cross-curve as pH decreased and total acidity increased during storage. PP tray showed the slowest change at $5^{\circ}C$ with time. The pH was initially 6.25, but decreased to 4.12~4.16 at 20 days, and total acidity showed a 4 to 4.8-fold increase after 20 days of storage compared to the initial value. During storage at $5^{\circ}C$, total bacterial count and lactic acid bacterial count rapidly increased after 4 days. The total bacterial quantity decreased after a period of time and there were differences according to packaging material; OPP/AL/PE packaging showed the most dramatic decrease. Change in microbial count mostly followed a similar pattern to that of total acidity for all packaging materials. Changes in the color of Kimchi liquid, when examined by color index in $L{\cdot}b$/a form, rapidly decreased over time, similar to pH. Small Ny/PE/PP and OPP/AL/PE packages of Kimchi were examined for changes in free volume inside the packaging. After 13 days of storage at $5^{\circ}C$, the volume was 243 mL, but storage at $20^{\circ}C$ resulted in a volume of 372 mL, a more than 1.5-fold increase in free volume. There were changes in the quality characteristics of small package Kimchi according to storage temperature and packaging material, and large changes in pH, total acidity, and microbial count were evident upon storage at $5^{\circ}C$ for 8 days, which was the optimum palatability period. Mostly, PP treatment showed the slowest quality changes upon storage at $5^{\circ}C$. However, due to small package Kimchi's fast consumption system, the appropriate choice of packaging material must consider the product's turnover ratio. Further, the varieties of small package Kimchi should be diversified according to different consumer preferences by offering Kimchi with different maturity levels. Further, since the leading consumer base ranges in age from the teens to thirties, the development of various products targeting such consumers is necessary.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.19 no.4
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• pp.81-99
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• 2020

Origin-destination data have been collected and utilized for demand analysis and service design in various fields such as public transportation and traffic operation. As the utilization of big data becomes important, there are increasing needs to store raw origin-destination data for big data analysis. However, it is not practical to store and analyze the raw data for a long period of time since the size of the data increases by the power of the number of the collection points. To overcome this storage limitation and long-period pattern analysis, this study proposes a methodology for compression and origin-destination data analysis with the compressed data. The proposed methodology is applied to public transit data of Sejong and Seoul. We first measure the reconstruction error and the data size for each truncated matrix. Then, to determine a range of principal components for removing random data, we measure the level of the regularity based on covariance coefficients of the demand data reconstructed with each range of principal components. Based on the distribution of the covariance coefficients, we found the range of principal components that covers the regular demand. The ranges are determined as 1~60 and 1~80 for Sejong and Seoul respectively.

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.3
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• pp.401-410
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• 2006

Dental caries results from localized demineralization of tooth enamel by acids of bacterial origin produced from the fermentation of dietary sugars. A group of related oral bacteria, collectively known as mutans streptococci, are implicated as the primary etiological agents of human caries. Within this group, Streptococcus mutans has been known as a causative agent for dental caries. As well as acid production yielding the demineralization of tooth enamel, adherence and colonization of S. mutans to the teeth are also important for their virulence Cell-surface fibrillar proteins, which mediate adherence to the salivary pellicle are virulence components of mutans streptococci, and primary candidates for a human caries vaccine. Here we report that the AgI/II gene from S. mutans GS-5 were cloned by PCR amplification of the bacterial chromosomal DNA and the integrity of cloned genes were confirmed by nucleotide sequencing. Sequence analyses showed the sequence alignment of 280 nucleotides between the cloned AgI/II and the reported sequence of S. mutans GS-5 showed the perfect match The cloned genes which signal nucleotide was truncated, were transferred into bacterial expression vector and the recombinant proteins were purified as His-tag fusion proteins In order to generate polyclonal antibodies against the recombinant proteins, AgI/II mr, some $100{\mu}g$ of the proteins was injected into mice three times. It can be used for an effective vaccine production to prevent dental caries caused by pathogenic S. mutans.

• Journal of Communications and Networks
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• v.10 no.1
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• pp.5-9
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• 2008

Researchers have investigated many upper bound techniques applicable to error probabilities on the maximum likelihood (ML) decoding performance of turbo-like codes and low density parity check (LDPC) codes in recent years for a long codeword block size. This is because it is trivial for a short codeword block size. Previous research efforts, such as the simple bound technique [20] recently proposed, developed upper bounds for LDPC codes and turbo-like codes using ensemble codes or the uniformly interleaved assumption. This assumption bounds the performance averaged over all ensemble codes or all interleavers. Another previous research effort [21] obtained the upper bound of turbo-like code with a particular interleaver using a truncated union bound which requires information of the minimum Hamming distance and the number of codewords with the minimum Hamming distance. However, it gives the reliable bound only in the region of the error floor where the minimum Hamming distance is dominant, i.e., in the region of high signal-to-noise ratios. Therefore, currently an upper bound on ML decoding performance for turbo-like code with a particular interleaver and LDPC code with a particular parity check matrix cannot be calculated because of heavy complexity so that only average bounds for ensemble codes can be obtained using a uniform interleaver assumption. In this paper, we propose a new bound technique on ML decoding performance for turbo-like code with a particular interleaver and LDPC code with a particular parity check matrix using ML estimated weight distributions and we also show that the practical iterative decoding performance is approximately suboptimal in ML sense because the simulation performance of iterative decoding is worse than the proposed upper bound and no wonder, even worse than ML decoding performance. In order to show this point, we compare the simulation results with the proposed upper bound and previous bounds. The proposed bound technique is based on the simple bound with an approximate weight distribution including several exact smallest distance terms, not with the ensemble distribution or the uniform interleaver assumption. This technique also shows a tighter upper bound than any other previous bound techniques for turbo-like code with a particular interleaver and LDPC code with a particular parity check matrix.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.4
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• pp.1908-1914
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• 2013

In this paper, we introduce a design method for a broadband planar quasi-Yagi antenna (QYA) for terrestrial digital television (DTV) reception. The coplanar strip line which feeds the driver dipole is connected to a microstrip line and is terminated by short circuit. By appending a wide strip-type rectangular director at a location close to the driver dipole, broadband impedance matching and gain enhancement in a high frequency region are obtained. The gain characteristics in a low frequency region are improved by adding a reflector formed by a truncated ground plane. To reduce the antenna size, the strip-type dipole and reflector are modified to half bow-tie (V)-shaped elements. The effects of various parameters on the antenna characteristics are examined. An antenna, as a design example for the proposed antenna, is designed for the operation in the frequency band of 470-806 MHz for terrestrial DTV. The optimized antenna is fabricated on an FR4 substrate and the experimental results show that the antenna has a good performance such as a frequency band of 450-848 MHz for a VSWR < 2, gain > 4.1 dBi, and front-to-back ratio > 10.4 dB.

• Development and Reproduction
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• v.22 no.2
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• pp.143-153
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• 2018

The large extracellular domain of glycoprotein hormone receptors is a unique feature within the G protein-coupled receptors (GPCRs) family. After interaction with the hormone, the receptor becomes coupled to Gs, which, in turn stimulates adenylyl cyclase and the production of cAMP. Potential phosphorylation sites exist in the C-terminal region of GPCRs. The experiments described herein represent attempts to determine the functions of the eel follicle-stimulating hormone receptor (eelFSHR). We constructed a mutant of eelFSHR, in which the C-terminal cytoplasmic tail was truncated at residue 614 (eelFSHR-t614). The eelFSHR-t614 lacked all potential phosphorylation sites present in the C-terminal region of eelFSHR. In order to obtain the eelFSHR ligand, we produced recombinant follicle-stimulating hormone ($rec-eelFSH{\beta}/{\alpha}$) in the CHO-suspension cells. The expression level was 2-3 times higher than that of the transient expression of eelFSH in attached CHO-K1 cells. The molecular weight of the $rec-eelFSH{\beta}/{\alpha}$ protein was identified to be approximately 34 kDa. The cells expressing eelFSHR-t614 showed an increase in agonist-induced cAMP responsiveness. The maximal cAMP responses of cells expressing eelFSHR-t614 were lower than those of cells expressing eelFSHR-wild type (eelFSHR-WT). The $EC_{50}$ following C-terminal deletion in CHO-K1 cells was approximately 60.4% of that of eelFSHR-WT. The maximal response in eelFSHR-t614 cells was also drastically lower than that of eelFSHR-WT. We also found similar results in PathHunter Parental cells expressing ${\beta}$-arrestin. Thus, these data provide evidence that the truncation of the C-terminal cytoplasmic tail phosphorylation sites in the eelFSHR greatly decreased cAMP responsiveness and maximal response in both CHO-K1 cells and Path-Hunter Parental cells expressing ${\beta}$-arrestin.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.40-45
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• 2001

Raw starch-digesting amylase (BF-2A, M.W. 93, 000 Da) from Bacillus circulans F-2 was converted to two components during digestion with subtilisin. Two components were separated and designated as BF-2A' (63, 000 Da) and BF-2B (30, 000 Da), respectively. BF-2A' exhibited the same hydrolysis curve for soluble starch as the original amylase (BF-2A). Moreover, the catalytic activities of original and modified enzymes were indistinguishable in $K_{m}$, Vmax for, and in their specific activity for soluble starch hydrolysis. However, its adsorbability and digestibility on raw starch was greatly decreased. Furthermore, the enzymatic action pattern on soluble starch was greatly different from that of the BF-2A. A smaller peptide (BF-2B) showed adsorb ability onto raw starch. By these results, it is suggested that the larger peptide (BF-2A') has a region responsible for the expression of the enzyme activity to hydrolyze soluble substrate, and the smaller peptide (BF-2B) plays a role on raw starch adsorption. A similar phenomenon is observed during limited proteinase K, thermolysin, and endopeptidase Glu-C proteolysis of the enzyme. Fragments resulting from proteolysis were characterized by immunoblotting with anti-RSDA. The proteolytic patterns resulting from proteinase K and subtilisin were the same, producing 63- and 30-kDa fragments. Similar patterns were obtained with endopeptidase Glu-C or thermolysin. All proteolytic digests contained a common, major 63-kDa fragment. Inactivation of RSDA activity results from splitting off the C-terminal domain. Hence, it seems probable that the protease sensitive locus is in a hinge region susceptible to cleavage. Extracellular enzymes immunoreactive toward anti-RSDA were detected through whole bacterial cultivation. Proteins of sizes 93-, 75-, 63-, 55-, 38-, and 31-kDa were immunologically identical to RSDA. Of these, the 75-kDa and 63-kDa proteins correspond to the major products of proteolysis with Glu-C and thermolysin. These results postulated that enzyme heterogeneity of the raw starch-hydrolysis system might arise from the endogeneous proteolytic activity of the bacterium. Truncated forms of rsda, in which the gene sequence encoding the conserved domain had been deleted, directed the synthesis of a functional amylase that did not bind to raw starch. This indicates that the conserved region of RSDA constitutes a raw starch-binding domain, which is distinct from the active centre. The possible role of this substrate-binding region is discussed.d.