• 한국세라믹학회지
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• 제35권11호
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• pp.1162-1170
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• 1998

PbO-TiO2-B2O3-BaO 계 유리상에서 PbTiO3의 결정화 메커니즘에 대한 이론적 고찰 및 전자현미경 관찰 그리고 결정화 기구의 열시차 분석에 대한 응용성을 조사하였다. 열시차 분석에 대한 Kissinger 식의 응용은 DTA 분석시 시료내 핵밀도가 변하지 않을 때 적용할 수 있으며, 표면결정화를 유도하기 위해 분말시료를 사용하는 경우도 활성화 에너지를 시료의 결정화 메커니즘에 크게 영향을 받는다. Ozawa 식에 의한 Avrami parameter n은 전자현미경 관찰에 의해 파악된 결정화메커니즘과 잘 일치하고 있었으며, 수정 Kissinger 식은 시료의 결정화 메커니즘을 반영하고 있어 시료의 전처리와 관계없이 참값의 활성화에너지를 구할 수 있다.

• Smart Structures and Systems
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• 제16권1호
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• pp.47-65
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• 2015

A novel finite element (FE) model updating method based on multi-resolution analysis (MRA) is proposed. The true stiffness of the FE model is considered as the superposition of two pieces of stiffness information of different resolutions: the pre-defined stiffness information and updating stiffness information. While the resolution of former is solely decided by the meshing density of the FE model, the resolution of latter is decided by the limited information obtained from the experiment. The latter resolution is considerably lower than the former. Second generation wavelet is adopted to describe the updating stiffness information in the framework of MRA. This updating stiffness in MRA is realized at low level of resolution, therefore, needs less number of updating parameters. The efficiency of the optimization process is thus enhanced. The proposed method is suitable for the identification of multiple irregular cracks and performs well in capturing the global features of the structural damage. After the global features are identified, a refinement process proposed in the paper can be carried out to improve the performance of the MRA of the updating information. The effectiveness of the method is verified by numerical simulations of a box girder and the experiment of a three-span continues pre-stressed concrete bridge. It is shown that the proposed method corresponds well to the global features of the structural damage and is stable against the perturbation of modal parameters and small variations of the damage.

• KDI Journal of Economic Policy
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• 제40권4호
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• pp.1-22
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• 2018

We estimate three continuous-time stochastic volatility models following the approach by Aït-Sahalia and Kimmel (2007) to compare the Korean and US stock markets. To do this, the Heston, GARCH, and CEV models are applied to the KOSPI 200 and S&P 500 Index. For the latent volatility variable, we generate and use the integrated volatility proxy using the implied volatility of short-dated at-the-money option prices. We conduct MLE in order to estimate the parameters of the stochastic volatility models. To do this we need the transition probability density function (TPDF), but the true TPDF is not available for any of the models in this paper. Therefore, the TPDFs are approximated using the irreducible method introduced in Aït-Sahalia (2008). Among three stochastic volatility models, the Heston model and the CEV model are found to be best for the Korean and US stock markets, respectively. There exist relatively strong leverage effects in both countries. Despite the fact that the long-run mean level of the integrated volatility proxy (IV) was not statistically significant in either market, the speeds of the mean reversion parameters are statistically significant and meaningful in both markets. The IV is found to return to its long-run mean value more rapidly in Korea than in the US. All parameters related to the volatility function of the IV are statistically significant. Although the volatility of the IV is more elastic in the US stock market, the volatility itself is greater in Korea than in the US over the range of the observed IV.

• 농업과학연구
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• 제49권4호
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• pp.795-807
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• 2022

The development and commercialization of industrial genetically modified (GM) organisms is actively progressing worldwide, highlighting an increased need for improved safety management protocols. We sought to establish an environmental monitoring method, using real-time polymerase chain reaction (PCR) and propidium monoazide (PMA) treatment to develop a quantitative detection protocol for living GM microorganisms. We developed a duplex TaqMan quantitative PCR (qPCR) assay to simultaneously detect the selectable antibiotic gene, ampicillin (AmpR), and the single-copy Escherichia coli taxon-specific gene, D-1-deoxyxylulose 5-phosphate synthase (dxs), using a direct cell suspension culture. We identified viable engineered E. coli cells by performing qPCR on PMA-treated cells. The theoretical cell density (true copy numbers) calculated from mean quantification cycle (Cq) values of PMA-qPCR showed a bias of 7.71% from the colony-forming unit (CFU), which was within ±25% of the acceptance criteria of the European Network of GMO Laboratories (ENGL). PMA-qPCR to detect AmpR and dxs was highly sensitive and was able to detect target genes from a 10,000-fold (10^-4) diluted cell suspension, with a limit of detection at 95% confidence (LOD_95%) of 134 viable E. coli cells. Compared to DNA-based qPCR methods, the cell suspension direct PMA-qPCR analysis provides reliable results and is a quick and accurate method to monitor living GM E. coli cells that can potentially be released into the environment.

• 한국어병학회지
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• 제37권1호
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• pp.9-15
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• 2024

An enzyme-linked immunosorbent assay (ELISA) with two Novirhabdovirus antigens (viral hemorrhagic septicemia virus, VHSV and infectious hematopoietic necrosis virus, IHNV) was used to detect specific antibodies against VHSV from olive flounder (Paralichthys olivaceus) sera. In ELISA plates with VHSV culture supernatants (VHSV-Ag plate), optical density (OD) values for sera from olive flounder with VHS history (VHS sera) ranged from 0.64±0.36, and those of sera from fish without VHS history (non-VHS sera) ranged from 0.26±0.26. In IHNV-Ag plate, the OD values (0.43±0.28) for VHS sera were quite low compared to those in VHSV-Ag plates, while the OD values for non-VHS sera were almost similar. When the OD values for each serum were calculated by subtracting the OD values in the IHNV-Ag plate from those in the VHSV-Ag plate, the corrected OD values were significantly different between VHS sera and non-VHS sera. The results were completely in line with fish histories of VHS epizootics. It was considered that the corrected OD values may represent the true values recognized by VHSV-specific antibodies.

• Journal of Periodontal and Implant Science
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• 제43권1호
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• pp.30-36
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• 2013

Purpose: Implant stability at the time of surgery is crucial for the long-term success of dental implants. Primary stability is considered of paramount importance to achieve osseointegration. The purpose of the present study was to investigate the correlation between the insertion torque and primary stability of dental implants using artificial bone blocks with different bone densities and compositions to mimic different circumstances that are encountered in routine daily clinical settings. Methods: In order to validate the objectives, various sized holes were made in bone blocks with different bone densities (#10, #20, #30, #40, and #50) using a surgical drill and insertion torque together with implant stability quotient (ISQ) values that were measured using the Osstell Mentor. The experimental groups under evaluation were subdivided into 5 subgroups according to the circumstances. Results: In group 1, the mean insertion torque and ISQ values increased as the density of the bone blocks increased. For group 2, the mean insertion torque values decreased as the final drill size expanded, but this was not the case for the ISQ values. The mean insertion torque values in group 3 increased with the thickness of the cortical bone, and the same was true for the ISQ values. For group 4, the mean insertion torque values increased as the cancellous bone density increased, but the correlation with the ISQ values was weak. Finally, in group 5, the mean insertion torque decreased as the final drill size increased, but the correlation with the ISQ value was weak. Conclusions: Within the limitations of the study, it was concluded that primary stability does not simply depend on the insertion torque, but also on the bone quality.